Mass Spectrometry in Structural Biology: Surface‐induced Dissociation/Ion Mobility of Protein Complexes

Surface collisions generate subcomplexes, which are then separated by ion mobility and dissociated into their individual subunitsviaa second stage of surface collisions to elucidate protein complex architecture and assembly.

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Surface induced dissociation as a tool to study membrane protein complexes

Chemical Communications ◽

10.1039/c6cc09606a ◽

2017 ◽

Vol 53 (21) ◽

pp. 3106-3109 ◽

Cited By ~ 21

Author(s):

Sophie R. Harvey ◽

Yang Liu ◽

Wen Liu ◽

Vicki H. Wysocki ◽

Arthur Laganowsky

Keyword(s):

Mass Spectrometry ◽

Membrane Protein ◽

Ion Mobility ◽

Protein Complexes ◽

Integral Membrane Protein ◽

Ion Mobility Mass Spectrometry ◽

Surface Induced Dissociation ◽

Membrane Protein Complexes

Native ion mobility mass spectrometry (MS) and surface induced dissociation (SID) are applied to study the integral membrane protein complexes AmtB and AqpZ.

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Uncovering the Stoichiometry ofPyrococcus furiosusRNase P, a Multi-Subunit Catalytic Ribonucleoprotein Complex, by Surface-Induced Dissociation and Ion Mobility Mass Spectrometry

Angewandte Chemie ◽

10.1002/ange.201405362 ◽

2014 ◽

Vol 126 (43) ◽

pp. 11667-11671 ◽

Cited By ~ 1

Author(s):

Xin Ma ◽

Lien B. Lai ◽

Stella M. Lai ◽

Akiko Tanimoto ◽

Mark P. Foster ◽

...

Keyword(s):

Mass Spectrometry ◽

Ion Mobility ◽

Ion Mobility Mass Spectrometry ◽

Ribonucleoprotein Complex ◽

Surface Induced Dissociation

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Structural insights into Escherichia coli phosphopantothenoylcysteine synthetase by native ion mobility–mass spectrometry

Biochemical Journal ◽

10.1042/bcj20190318 ◽

2019 ◽

Vol 476 (21) ◽

pp. 3125-3139 ◽

Cited By ~ 2

Author(s):

Daniel Shiu-Hin Chan ◽

Jeannine Hess ◽

Elen Shaw ◽

Christina Spry ◽

Robert Starley ◽

...

Keyword(s):

Mass Spectrometry ◽

Escherichia Coli ◽

Structural Biology ◽

Ion Mobility ◽

Biosynthetic Pathway ◽

Screening Tool ◽

Native Mass Spectrometry ◽

Ion Mobility Mass Spectrometry ◽

E Coli ◽

Structural Insights

Abstract CoaBC, part of the vital coenzyme A biosynthetic pathway in bacteria, has recently been validated as a promising antimicrobial target. In this work, we employed native ion mobility–mass spectrometry to gain structural insights into the phosphopantothenoylcysteine synthetase domain of E. coli CoaBC. Moreover, native mass spectrometry was validated as a screening tool to identify novel inhibitors of this enzyme, highlighting the utility and versatility of this technique both for structural biology and for drug discovery.

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Ion Mobility–Mass Spectrometry Reveals Long-Lived, Unfolded Intermediates in the Dissociation of Protein Complexes

Angewandte Chemie International Edition ◽

10.1002/anie.200702161 ◽

2007 ◽

Vol 46 (42) ◽

pp. 8001-8004 ◽

Cited By ~ 176

Author(s):

Brandon T. Ruotolo ◽

Suk-Joon Hyung ◽

Paula M. Robinson ◽

Kevin Giles ◽

Robert H. Bateman ◽

...

Keyword(s):

Mass Spectrometry ◽

Ion Mobility ◽

Protein Complexes ◽

Ion Mobility Mass Spectrometry

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EM∩IM: software for relating ion mobility mass spectrometry and electron microscopy data

The Analyst ◽

10.1039/c5an01636c ◽

2016 ◽

Vol 141 (1) ◽

pp. 70-75 ◽

Cited By ~ 11

Author(s):

Matteo T. Degiacomi ◽

Justin L. P. Benesch

Keyword(s):

Electron Microscopy ◽

Mass Spectrometry ◽

Structural Biology ◽

Cross Sections ◽

Ion Mobility ◽

Ion Mobility Mass Spectrometry ◽

Density Maps ◽

Transmission Electron ◽

Microscopy Data ◽

Means Of Transmission

EM∩IM enables the calculation of collision cross-sections from electron density maps obtained, for example, by means of transmission electron microscopy. This capability will further aid the integration of ion mobility mass spectrometry with modern structural biology.

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