Combined cDNA array/RT‐PCR analysis of gene expression profile in rat gastrocnemius muscle: relation to its adaptive function in energy metabolism during fasting

2003 ◽  
Vol 18 (2) ◽  
pp. 1-22 ◽  
Author(s):  
Pieter Lange ◽  
Maurizio Ragni ◽  
Elena Silvestri ◽  
Maria Moreno ◽  
Luigi Schiavo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Energy Metabolism ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Gastrocnemius Muscle ◽  
Cdna Array ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Adaptive Function

scholarly journals Gene Expression Profile of Colon Mucosa after Cytotoxic Insult in wt and Apc-Mutated Pirc Rats: Possible Relation to Resistance to Apoptosis during Carcinogenesis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Angelo Pietro Femia ◽  
Cristina Luceri ◽  
Maura Lodovici ◽  
Stefania Crucitta ◽  
Giovanna Caderni
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Antioxidant Status ◽  
Genotoxic Stress ◽  
Rt Pcr ◽  
Induced Genes ◽  
Stress Related Genes ◽  
Microarray Technique ◽  
Unsupervised Cluster Analysis

Apc-mutated Pirc rats, spontaneously developing intestinal tumours, are resistant to 1,2-dimethylhydrazine- (DMH-) induced colon apoptosis. To understand this phenomenon, we analyzed the expression of genotoxic stress-related genes Mgmt, Gsta1, and Gstp1 in the colon of wt and Pirc rats in basal conditions and 24 h after DMH; plasmatic oxidant/antioxidant status was also evaluated. After DMH, Mgmt expression was increased in both genotypes but significantly only in wt rats; Gsta1 expression was significantly increased in both genotypes. Gstp1 expression did not vary after DMH but was lower in Pirc rats. Moreover, for each genotype, we studied by microarray technique whole gene expression profile after DMH. By unsupervised cluster analysis, 28 genes were differentially modulated between the two genotypes. Among them were interferon-induced genes Irf7, Oas1a, Oasl2, and Isg15 and the transcription factor Taf6l, overexpressed in DMH-treated wt rats and unchanged in Pirc rats. RT-PCR confirmed their overexpression in DMH-treated wt rats and showed a slighter variation in DMH-treated Pirc rats. Taken together, despite a blunted induction of Irf7, Oas1a, and Mgmt, defective apoptosis in Pirc rats 24 h after DMH is not mirrored by major differences in gene expression compared with wt rats.

scholarly journals Application of cDNA array for studying the gene expression profile of mature appressoria of Magnaporthe grisea

2007 ◽  
Vol 8 (2) ◽  
pp. 88-97 ◽  
Author(s):  
Qing-chao Jin ◽  
Hai-tao Dong ◽  
You-liang Peng ◽  
Bao-shan Chen ◽  
Jing Shao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Magnaporthe Grisea ◽  
Cdna Array

Gene expression profile in mouse myocardium after ischemia

2000 ◽  
Vol 2 (3) ◽  
pp. 93-100 ◽  
Author(s):  
DEBORAH LYN ◽  
XIAOWEI LIU ◽  
NICOLE A. BENNETT ◽  
NERIMIAH L. EMMETT
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Stress Responses ◽  
Muscle Development ◽  
Cardiac Injury ◽  
Cdna Array ◽  
Polymerase Chain Reaction Analysis ◽  
Molecular Profile ◽  
Mouse Heart

Lyn, Deborah, Xiaowei Liu, Nicole A. Bennett, and Nerimiah L. Emmett. Gene expression profile in mouse myocardium after ischemia. Physiol Genomics 2: 93–100, 2000.—This study was designed to elaborate a molecular profile of expressed genes during ischemic injury to the mouse heart after surgical constriction of the left coronary artery without reperfusion. A mouse cDNA array containing 588 known genes was used to compare gene expression in heart RNA after 24-h ischemia with control tissue. Alterations in gene expression on the array were supported by relative reverse transcription-polymerase chain reaction analysis after timed periods of ischemia. Decreased levels of the cell cycle regulator p18ink4 and the oxidative responsive gene glutathione S-transferase were accompanied by an upregulation of the genes associated with cardiac muscle development, α-myosin heavy chain and fetal myosin alkali light chain. Other stress responses elicited by cardiac injury included an induction of Egr-1 and Egr-3 transcription factors, as well as the apoptotic regulator Bax. Altogether, these findings indicate that expression of genes associated with a fetal transcription program may be involved with the post ischemic remodeling process in heart ventricles.

Gene Expression Profile Predicts Patient Survival of Gastric Cancer After Surgical Resection

2005 ◽  
Vol 23 (29) ◽  
pp. 7286-7295 ◽  
Author(s):  
Chiung-Nien Chen ◽  
Jen-Jen Lin ◽  
Jeremy J. W. Chen ◽  
Po-Huang Lee ◽  
Ching-Yao Yang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Prediction Model ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Good Survival ◽  
Survival Prediction ◽  
Rt Pcr ◽  
Poor Survival ◽  
Gastric Cancer Patients

Purpose This study was conducted to characterize gene expression profile of survival in patients with surgically curable gastric cancer by using an in-house membrane microarray and developing a survival prediction model. Materials and Methods Data of cDNA microarrays were obtained from 18 pairs of cancerous and noncancerous gastric tissues. Nine patients who survived > 30 months were identified as good survival, and the other nine, who survived < 12 months, were identified as poor survival. Supervised analysis was performed to identify a gene expression profile by good and poor survival. Semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to confirm the microarray data in 10 patients with sufficient RNA. Using these 10 patients and another 10 patients selected randomly from 40 newly enrolled patients as the training group, the RT-PCR status of the confirmed genes was used for predicting good versus poor survival. Finally, the prediction model was tested in the remaining 30 newly enrolled gastric cancer patients. Results A survival prediction model consisting of three genes (CD36, SLAM, PIM-1) was developed. This model could correctly predict poor or good survival in 23 (76.7%) of 30 newly enrolled patients, and yielded a specificity of 80% and a sensitivity of 73.3%. The survival rate of the patients predicted to have good survival was significantly higher than that of those predicted to have poor survival in the test group as a whole (N = 30; P = .00531) and in stage III patients (n = 16; P = .04467). Conclusion The semiquantitative RT-PCR gene expression profiling of three genes extracted from microarray study can accurately predict surgery-related outcome in gastric cancer patients.

scholarly journals Comprehensive gene expression profile of the adult human renal cortex: Analysis by cDNA array hybridization

2000 ◽  
Vol 57 (4) ◽  
pp. 1452-1459 ◽  
Author(s):  
Naohiro Yano ◽  
Masayuki Endoh ◽  
Kimberly Fadden ◽  
Hiroshi Yamashita ◽  
Agnes Kane ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Renal Cortex ◽  
Cdna Array ◽  
Adult Human ◽  
Array Hybridization

Gene Expression Profile in Human Late Radiation Enteritis Obtained by High-Density cDNA Array Hybridization

2004 ◽  
Vol 161 (3) ◽  
pp. 299-311 ◽  
Author(s):  
Marie-Catherine Vozenin-Brotons ◽  
Fabien Milliat ◽  
Christine Linard ◽  
Carine Strup ◽  
Agnès François ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Cdna Array ◽  
High Density ◽  
Radiation Enteritis ◽  
Array Hybridization

scholarly journals Anaplasma phagocytophilumandAnaplasma marginaleElicit Different Gene Expression Responses in Cultured Tick Cells

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Zorica Zivkovic ◽  
Edmour F. Blouin ◽  
Raúl Manzano-Roman ◽  
Consuelo Almazán ◽  
Victoria Naranjo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Life Cycle ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Anaplasma Phagocytophilum ◽  
Ixodes Scapularis ◽  
Host Cells ◽  
Rt Pcr ◽  
Infected Tick ◽  
Tick Survival

The genusAnaplasma(Rickettsiales: Anaplasmataceae) includes obligate tick-transmitted intracellular organisms,Anaplasma phagocytophilumandAnaplasma marginalethat multiply in both vertebrate and tick host cells. Recently, we showed thatA. marginaleaffects the expression of tick genes that are involved in tick survival and pathogen infection and multiplication. However, the gene expression profile inA. phagocytophilum-infected tick cells is currently poorly characterized. The objectives of this study were to characterize tick gene expression profile inIxodes scapularisticks and cultured ISE6 cells in response to infection withA. phagocypthilumand to compare tick gene expression responses inA. phagocytophilum- andA. marginale-infected tick cells by microarray and real-time RT-PCR analyses. The results of these studies demonstrated modulation of tick gene expression byA. phagocytophilumand provided evidence of different gene expression responses in tick cells infected withA. phagocytophilumandA. marginale. These differences inAnaplasma-tick interactions may reflect differences in pathogen life cycle in the tick cells.

scholarly journals Mulberry Extracts Alleviate Aβ25–35-Induced Injury and Change the Gene Expression Profile in PC12 Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Nan Song ◽  
Hongpeng Yang ◽  
Wei Pang ◽  
Zhiwei Qie ◽  
Hao Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pc12 Cells ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Binding Activity ◽  
Peptidase Activity ◽  
Mrna Levels ◽  
Rt Pcr

Mulberry, which contained high amounts of anthocyanins, has been used in traditional Chinese medicine. Mulberry fruit extracts (ME) have demonstrated the antioxidant activity and neuroprotection. The study was to investigate the neuroprotective efficacy of ME againstβ-amyloid 25–35- (Aβ25–35-) induced PC12 cells injury. Cells preincubated with or without ME (200 μg/mL) for 24 h were treated with Aβ25–35(20 μmol/L) for another 24 h. Cell viability was assessed by MTT, gene expression profiles were examined by cDNA microarrays, and RT-PCR were used to confirm the results of microarray assays. ME pretreatment was found to neutralize the cytotoxicity and prevent Aβ25–35-induced cells injury. Analyses of gene expression profile revealed that genes involving cell adhesion, peptidase activity, cytokine activity, ion binding activity, and angiogenesis regulation were significantly modulated by ME pretreatment. Among those genes, Apaf1, Bace2, and Plcb4 were enriched in the “Alzheimer’s disease-reference pathway” and downregulated after ME intervention. RT-PCR results showed that ME preincubation could significantly inhibit Aβ25–35increased mRNA levels of these three genes. Overall, ME pretreatment could substantially alleviate PC12 cells injury and downregulate expression of AD-related genes, such as Apaf1, Bace2, and Plcb4. This study has a great nutrigenomics interest and brings new and important light in the field of AD intervention.

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