Processing of the betaamyloid precursor protein in ex vivo human brain cells

Neuroreport ◽  
1999 ◽  
Vol 10 (18) ◽  
pp. 3875-3879 ◽  
Author(s):  
Sonia S. Jung ◽  
Neil R. Cashman
Keyword(s):  
Human Brain ◽  
Ex Vivo ◽  
Precursor Protein ◽  
Brain Cells

FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells

2017 ◽  
pp. 27-41 ◽  
Author(s):  
Yuri B. Yurov ◽  
Svetlana G. Vorsanova ◽  
Ilia V. Soloviev ◽  
Alexei M. Ratnikov ◽  
Ivan Y. Iourov
Keyword(s):  
Human Brain ◽  
Chromosomal Mosaicism ◽  
Brain Cells

scholarly journals Zika virus impairs growth in human neurospheres and brain organoids

2016 ◽  
Author(s):  
Patricia P Garcez ◽  
Erick C Loiola ◽  
Rodrigo F Madeiro da Costa ◽  
Luiza Higa ◽  
Pablo Trindade ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Brain ◽  
Brain Development ◽  
Zika Virus ◽  
Brain Cells ◽  
Zikv Infection ◽  
Human Neural Stem Cells ◽  
Human Brain Development

Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased dramatically in Brazil; however, causality between the widespread epidemic and malformations in fetal brains has not been confirmed. Here, we examine the effects of ZIKV infection in human neural stem cells growing as neurospheres and cerebral organoids. Using immunocytochemistry and electron microscopy, we show that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and cerebral organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.

scholarly journals Multicellular ‘hotspots’ harbour high-grade potential in lower-grade gliomas

2021 ◽  
Author(s):  
Alastair J Kirby ◽  
José P Lavrador ◽  
Istvan Bodi ◽  
Francesco Vergani ◽  
Ranjeev Bhangoo ◽  
...  
Keyword(s):  
Human Brain ◽  
Brain Tissue ◽  
Aminolevulinic Acid ◽  
Ex Vivo ◽  
Brain Slices ◽  
Glioma Cells ◽  
Malignant Progression ◽  
Lower Grade ◽  
High Grade ◽  
Human Brain Tissue

Abstract Background Lower-grade gliomas may be indolent for many years before developing malignant behaviour. The reasons mechanisms underlying malignant progression remain unclear. Methods We collected blocks of live human brain tissue donated by people undergoing glioma resection. The tissue blocks extended through the peritumoral cortex and into the glioma. The living human brain tissue was cut into ex vivo brain slices and bathed in 5-aminolevulinic acid (5-ALA). High-grade glioma cells avidly take up 5-aminolevulinic acid (5-ALA) and accumulate high levels of the fluorescent metabolite, Protoporphyrin IX (PpIX). We exploited the PpIX fluorescence emitted by higher-grade glioma cells to investigate the earliest stages of malignant progression in lower-grade gliomas. Results We found sparsely-distributed ‘hot-spots’ of PpIX-positive cells in living lower-grade glioma tissue. Glioma cells and endothelial cells formed part of the PpIX hotspots. Glioma cells in PpIX hotspots were IDH1 mutant and expressed nestin suggesting they had acquired stem-like properties. Spatial analysis with 5-ALA conjugated quantum dots indicated that these glioma cells replicated adjacent to blood vessels. PpIX hotspots formed in the absence of angiogenesis. Conclusion Our data show that PpIX hotspots represent microdomains of cells with high-grade potential within lower-grade gliomas and identify locations where malignant progression could start.

scholarly journals Alzheimer beta/A4 amyloid precursor protein in human brain: aging-associated increases in holoprotein and in a proteolytic fragment.

1991 ◽  
Vol 88 (20) ◽  
pp. 8910-8914 ◽  
Author(s):  
C. Nordstedt ◽  
S. E. Gandy ◽  
I. Alafuzoff ◽  
G. L. Caporaso ◽  
K. Iverfeldt ◽  
...  
Keyword(s):  
Amyloid Precursor Protein ◽  
Human Brain ◽  
Brain Aging ◽  
Precursor Protein ◽  
Proteolytic Fragment

Real-Time Ex-Vivo Magnetic Resonance Image—Guided Dissection of Human Brain White Matter: A Proof-of-Principle Study

2019 ◽  
Vol 125 ◽  
pp. 198-206 ◽  
Author(s):  
Giacomo Bertolini ◽  
Emanuele La Corte ◽  
Domenico Aquino ◽  
Elena Greco ◽  
Zefferino Rossini ◽  
...  
Keyword(s):  
White Matter ◽  
Magnetic Resonance ◽  
Human Brain ◽  
Real Time ◽  
Magnetic Resonance Image ◽  
Ex Vivo ◽  
Image Guided ◽  
Brain White Matter ◽  
Proof Of Principle
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