Mast Cells and Nerve Fibers in Interstitial Cystitis (IC)

1998 ◽  
pp. 2252
Author(s):  
M. A. Hofmeister ◽  
F. He ◽  
T. L. Ratliff ◽  
T. Mahoney ◽  
M. J. Becich
Keyword(s):  
Mast Cells ◽  
Interstitial Cystitis ◽  
Nerve Fibers

Ultrastructural evidence for bladder MAST cell degranulation and their imminent association with nerve fibers in patients with interstitial cystitis

1994 ◽  
Vol 52 ◽  
pp. 264-265
Author(s):  
F. He ◽  
M. Hofmeister ◽  
T. Ratliff ◽  
M. Becich
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Interstitial Cystitis ◽  
Ultrastructural Level ◽  
Nerve Fibers ◽  
Morphological Description ◽  
Electron Microscopic ◽  
Human Bladder ◽  
Surgical Biopsy ◽  
Transmission Electron

The ultrastructure of human mast cells (HMCs) in various diseases has been well documented; however, detailed morphological description of human bladder mast cells (HBMCs) in patients with interstitial cystitis (IC) is incomplete. The present study was undertaken to reveal any morphological modifications of human bladder mast cells (HBMCs) from the IC patients and to investigate the spatial relationships between nerve fibers and these mast cells at the ultrastructural level.Fresh-fixed surgical biopsy specimens from IC patients as well as paraffin blocks of IC retrieved through our medical record archival system were processed with routine and deparaffinization methods for transmission electron microscopy (1). No differences in mast cell morphology were noticed between the fresh-fixed or paraffin embedded tissues.The general electron microscopic appearances of the HBMCs in IC located in both mucosa and muscle layers were similar to those of HMCs observed in other sites where mastocytosis may occur due to various pathological processes (Fig. 1).

scholarly journals Pathophysiological Roles of Neuro-Immune Interactions between Enteric Neurons and Mucosal Mast Cells in the Gut of Food Allergy Mice

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1586
Author(s):  
Tomoe Yashiro ◽  
Hanako Ogata ◽  
Syed Faisal Zaidi ◽  
Jaemin Lee ◽  
Shusaku Hayashi ◽  
...  
Keyword(s):  
Food Allergy ◽  
Mast Cells ◽  
Imaging System ◽  
Receptor Gene ◽  
Nerve Fibers ◽  
Enteric Neurons ◽  
Food Allergies ◽  
Adenosine A3 Receptor ◽  
Myenteric Neurons ◽  
Mucosal Mast Cells

Recently, the involvement of the nervous system in the pathology of allergic diseases has attracted increasing interest. However, the precise pathophysiological role of enteric neurons in food allergies has not been elucidated. We report the presence of functional high-affinity IgE receptors (FcεRIs) in enteric neurons. FcεRI immunoreactivities were observed in approximately 70% of cholinergic myenteric neurons from choline acetyltransferase-eGFP mice. Furthermore, stimulation by IgE-antigen elevated intracellular Ca2+ concentration in isolated myenteric neurons from normal mice, suggesting that FcεRIs are capable of activating myenteric neurons. Additionally, the morphological investigation revealed that the majority of mucosal mast cells were in close proximity to enteric nerve fibers in the colonic mucosa of food allergy mice. Next, using a newly developed coculture system of isolated myenteric neurons and mucosal-type bone-marrow-derived mast cells (mBMMCs) with a calcium imaging system, we demonstrated that the stimulation of isolated myenteric neurons by veratridine caused the activation of mBMMCs, which was suppressed by the adenosine A3 receptor antagonist MRE 3008F20. Moreover, the expression of the adenosine A3 receptor gene was detected in mBMMCs. Therefore, in conclusion, it is suggested that, through interaction with mucosal mast cells, IgE-antigen-activated myenteric neurons play a pathological role in further exacerbating the pathology of food allergy.

Functional relationships between sensory nerve fibers and mast cells of dura mater in normal and inflammatory conditions

Neuroscience ◽  
1997 ◽  
Vol 77 (3) ◽  
pp. 829-839 ◽  
Author(s):  
V Dimitriadou ◽  
A Rouleau ◽  
M.D Trung Tuong ◽  
G.J.F Newlands ◽  
H.R.P Miller ◽  
...  
Keyword(s):  
Mast Cells ◽  
Dura Mater ◽  
Sensory Nerve ◽  
Nerve Fibers ◽  
Functional Relationships ◽  
Inflammatory Conditions

Electron Microscopical and Immunohistochemical Studies on the Induction of "Qi" Employing Needling Manipulation

1992 ◽  
Vol 20 (01) ◽  
pp. 25-35 ◽  
Author(s):  
Michio Kimura ◽  
Kazuo Tohya ◽  
Kyo-ichi Kuroiwa ◽  
Hirohisa Oda ◽  
E. Christo Gorawski ◽  
...  
Keyword(s):  
Mast Cells ◽  
Nerve Fiber ◽  
Functional Relationship ◽  
Nerve Fibers ◽  
Elastic Fibers ◽  
Electron Microscopical Analysis ◽  
Calcitonin Gene ◽  
Transparent Materials ◽  
Electron Microscopical ◽  
Immunohistochemical Studies

During a sparrow-pecking and twisting-needle manipulation to the acupoints BL 23, 24 and 25 for an induction of "Qi", it was found that some transparent materials were binding to the needles after removed from the volunteer's skin. Electron-microscopical analysis of the transparent materials revealed that they corresponded to the injured fascia made up of collagen fibers, elastic fibers, fibroblasts, adipocytes and mast cells. Rarely were nerve fiber-like structures observed in the materials. Immunohistochemically, calcitonin gene-related peptide-positive nerve fibers could be demonstrated in the acupoint BL 24 associated fascia. A possible functional relationship between the needle manipulation and the induction of Qi-sensation is discussed along with the acupoint tissue constitution.

scholarly journals Relationships between urinary neural and immune factors in the patients with interstitial cystitis/ bladder painful syndrome

2020 ◽  
Vol 22 (5) ◽  
pp. 879-886
Author(s):  
R. F. Sholan
Keyword(s):  
Mast Cells ◽  
Interstitial Cystitis ◽  
Pearson Correlation ◽  
Control Level ◽  
Clinical Manifestations ◽  
Control Group ◽  
Bladder Tissue ◽  
Bladder Pain ◽  
Group I ◽  
Group Ii

The purpose of this work was to study the relationships between urinary cytokines, mast cells and nerve growth factor (NGF) in the patients with interstitial cystitis/bladder pain syndrome (IC/BPS). Sixty-eight women with clinically diagnosed IC/BPS were under study. Their mean age was 54.2±12.4 years. Urinary concentrations of interleukins (IL-1β, IL-6, IL-8), tumor necrosis factor-α (TNFα), and NGF were determined by ELISA technique. Mast cells were identified in biopsies of mucous membranes from urinary bladder harvested during cystoscopy. Statistical evaluation was performed by Statistica program in Microsoft Excel. Pearson correlation quotients were calculated. Depending on the type of IC/BPS, the patients were divided into 2 groups: group I included 36 patients with classic type of disease; group II comprised 32 patients with non-ulcer type of IC/BPS. No significant differences were revealed between the groups. In 13.9% of patients from group I, the onset of clinical manifestations of the disease was observed at the age of less than 40 years; in group II, 28.1% of the examined mentioned appearance of the disease symptoms at this age. The levels of IL-1β in the patients from group I was 2.4 times higher than in controls (p < 0.05). IL-6, IL-8 and TNFα concentrations exceeded control values by 2.0 (p < 0.05), 2.5 (p < 0.05) and 2.0 times (p < 0.05), respectively. In the patients from group II, the content of IL-1β, IL-6, IL-8 and TNFα was 2.4 (p < 0.05), 2.0 (p < 0.05), 2.0 (p < 0.05) and 1.9 (p < 0.05) times higher than in the control group, respectively. There were no significant differences between groups I and II, in IL-1β, IL-6, and TNFα levels, except of IL-8 in women of group I that was 20.3% higher than in group II. The urinary NGF level in the patients with IC/BPS exceeded the control level 1.6 times (p < 0.05) for group I, and 1.5 times (p < 0.05) for group II. The number of mast cells in the patients of group I was significantly higher than in controls and in group II, i.e., 1.6 (p < 0.05) and 1.4 times (p < 0.05), respectively. In most cases, a direct weak correlation was revealed between the indices. Only in group I, a moderate correlation (r = + 0.508) could be detected between IL-1β and mast cells. Determination of cytokine levels allows to detect activation of inflammatory cells in bladder tissue and provides an opportunity for developing diagnostic strategies. Increased numbers of mast cells may indicate the importance of these cells in the disease progression, whereas elevated levels of NGF in urine suggests that IC/BPS may be caused by chronic inflammation.

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