Urinary Tract Infections: Need for Pure Culture Technology in Clinical Laboratory Diagnosis

1991 ◽  
Vol 84 (4) ◽  
pp. 539-540
Author(s):  
Paul Fugazzotto
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Pure Culture ◽  
Clinical Laboratory ◽  
Laboratory Diagnosis ◽  
Tract Infections ◽  
Culture Technology

Application of quantitative multiplex real-time polymerase chain reaction for identification of causative agents of urinary tract infections in pregnancy

2014 ◽  
Vol 5 (3) ◽  
pp. 37-41
Author(s):  
Tatyana Alekseyevna Khusnutdinova ◽  
Yuliya Anatolyevna Savochkina ◽  
Aleksandr Yevgenyevich Gushchin ◽  
Yelena Vasilyevna Shipitsyna ◽  
Alevtina Mikhailovna Savicheva
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Bacterial Infections ◽  
Asymptomatic Bacteriuria ◽  
Laboratory Diagnosis ◽  
Obstetrical Care ◽  
Bacterial Proliferation ◽  
Causative Agents ◽  
Tract Infections ◽  
In Pregnancy

Urinary tract infections (UTIs) represent the most common bacterial infections and often complicate pregnancy. UTIs in pregnancy are classified by site of bacterial proliferation as follows: asymptomatic bacteriuria, cystitis, pyelonephritis. Screening for asymptomatic bacteriuria is a standard of obstetrical care and is included in most international guidelines. The urine microbiologic culture is considered the gold standard for laboratory diagnosis of UTIs. Molecular methods enable to rapidly detect, identify and quantitate causative agents of UTIs in urine and are effective alternative to traditional bacteriological methods.

scholarly journals Laboratory Diagnosis of Urinary-tract Infections

BMJ ◽  
1951 ◽  
Vol 1 (4715) ◽  
pp. 1119-1123 ◽  
Author(s):  
J. B. Enticknap ◽  
B. J. Stephens
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Laboratory Diagnosis ◽  
Tract Infections

scholarly journals Incidence of extended spectrum Beta-Lactamase producing Klebsiella pneumoniae among patients with urinary tract infections in Kano Metropolis Nigeria

2020 ◽  
Vol 12 (1) ◽  
pp. 139-144
Author(s):  
A. Mohammed ◽  
A.M Magashi ◽  
M. Yushau
Keyword(s):  
Urinary Tract ◽  
Klebsiella Pneumoniae ◽  
Urinary Tract Infections ◽  
Clinical Laboratory ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Synergy Test ◽  
Tract Infections ◽  
Pharmacologically Active

Extended Spectrum Beta-Lactamase (ESBLs) production is one of the ways by which bacteria become resistant to antibiotics and pathogens of UTIs such as Klebsiella pneumoniae have been incriminated at global scale. This study was conducted to investigate the incidence of Extended Spectrum Beta lactamase producing Klebsiella pneumoniae from Urinary Tract Infections in Kano metropolis. The work involved One hundred and fourty seven K. Pneumoniae isolates obtained from patients with suspected urinary tract infections were studied from January to July 2017. The identity of the isolates was confirmed using MicrogenTMGnA + B-ID System. Antibiotic susceptibility testing was carried out using the Kirby-Bauer Disc Diffusion Technique. Screening for ESBLs production was done using Clinical Laboratory Standards Institute breakpoint. Suspected ESBLs producers were subjected to confirmation using Double Disc Synergy Test. Standard Discs of Augmentin (AMC 30µG Oxoid England), Ceftazidime (CAZ 30µG, Oxoid England) and Cefotaxime (CTX 30µG, Oxoid England) were used for the screening and confirmation. Accordingly, Multidrug Resistant K. pneumoniae were found to be 63.3% and all were ESBLs producers. The Double Disc Synergy Test however confirmed 6.8% ESBLs producing K. pneumoniae. Antimicrobial sensitivity of the ESBLs producing organisms showed 100% resistance to Augmentin, ceftriaxone, ceftazidime, cefotaxime while resistance to gentamicin was 91.5%, chloramphenicol 23.4%, Nitrofurantoin 61.7%, Ciprofloxacin 93.6% and cotrimoxazole 95.7%. However, Imipenem was the most pharmacologically active drug. ESBL producing K. pneumoniae are incident in Kano and are resistant to commonly prescribed antibiotics. We, therefore, suggest screening and confirmation for ESBL in any attempt to treat UTIs due to such pathogens Keywords: Extended Spectrum Beta Lactamases, K. pneumoniae, Urinary Tract Infection, Incidence, Kano

scholarly journals 1697. The Burden Of Multidrug-Resistant Urinary Tract Infections

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S831-S831
Author(s):  
Fatma Hammami ◽  
Makram Koubaa ◽  
Amal Chakroun ◽  
Khaoula Rekik ◽  
Fatma Smaoui ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Antimicrobial Resistance ◽  
Urinary Tract Infections ◽  
Clinical Laboratory ◽  
Antibiotic Consumption ◽  
Multidrug Resistant ◽  
Disease Evolution ◽  
Significant Difference ◽  
The Mean ◽  
Tract Infections

Abstract Background Urinary tract infections (UTIs) are the most common reason for consultation and for antibiotic use. Many factors interfere and increase the risk for antimicrobial resistance. We aimed to study the clinical, laboratory and evolutionary particularities associated with multidrug-resistant (MDR) UTIs. Methods We conducted a retrospective study including all patients hospitalized for UTIs in the infectious diseases department between 2011 and 2018. Results A total of 867 cases of UTIs were included in the study, among which 407 cases (46.9%) were MDR. There were 306 males (35.3%). The mean age was 53±21 years. Overall, MRD UTIs were significantly associated to male gender (39.1% vs 32%; p=0.02). Patients aged ≥65 years were significantly more affected with MRD UTIs (54.5% vs 36.5%; p< 0.001). Previous medical history of diabetes (38.1% vs 24.6%; p< 0.001), antibiotic consumption (30.7% vs 13%; p< 0.001) and surgical intervention of the urinary tract (13% vs 5.4%; p< 0.001) were significantly associated with MDR UTIs. The mean delay to hospitalization was significantly longer among MDR UTIs cases (5[3-10 days] vs 3[2-7 days]; p< 0.001). In total, MDR UTIs were more frequently documented to Klebsiella pneumoniae (19.4% vs 12%; p=0.002). Comparison of the disease evolution showed that MRD UTIs were significantly associated with complications (9.1% vs 5.2%; p=0.02), recurrence (4.4% vs 1.5%; p=0.01) and death (2.2% vs 0.4%; p=0.02). As to laboratory investigations and antibiotic duration, no significant difference was noted. Conclusion Our study showed that MDR UTIs were associated with not only complications, but also with a poor prognosis. The continuous surveillance for antimicrobial resistance and the rational use of antibiotics are crucial in order to improve the prognosis. Disclosures All Authors: No reported disclosures

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