Diagnostic potential of fluorescence of formalin-fixed paraffin-embedded malignant melanoma and pigmented skin lesions: quantitative study of fluorescence intensity using fluorescence microscope and digital imaging

Papillomavirus (PV) mainly infects the squamous epithelium and may potentially lead to benign or even malignant cutaneous lesions. However, the malignant transforming ability has been identified in several types of PVs. In humans, papillomavirus (HPV) type 16 and 18 are the most prevalent causative agents of cervical cancer. Therefore, vaccines are being developed to protect against these types. For dogs, there have been limited investigations into the association of different canine papillomavirus (CPV) genotypes with malignant lesions. Understanding the high-risk CPV genotype(s) responsible for these malignant lesions would contribute to the development of interventions for preventing CPV-induced carcinomas. In the present study, a retrospective cohort of 102 pathologically confirmed papillomas and 212 squamous cell carcinomas (SCCs) were included. The viral genome and antigens in the formalin-fixed paraffin-embedded (FFPE) tissues were detected using PCR targeting pan PV E1 and COPV L1 genes and by immunohistochemistry staining (IHC), respectively. PVs were successfully detected from 11 FFPE cutaneous tissues and four oral tissues using pan PV E1- and COPV L1-based PCR, respectively. After sequencing, CPV 1, CPV 2, and CPV 6 were detected in the benign lesions using PCR and were confirmed through IHC. While CPV 9 and CPV 15 were first detected in the SCCs of dogs, CPV 16 was most often detected in SCC specimens. The association and confirmative demonstration of viral genes and intralesional antigens of CPV 9, CPV 15, and CPV 16 in SCCs highlight the potential risk of these genotypes of CPVs in malignant transformation.

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Multi-center evaluation of the novel fully-automated PCR-based Idylla™ BRAF Mutation Test on formalin-fixed paraffin-embedded tissue of malignant melanoma

Experimental and Molecular Pathology ◽

10.1016/j.yexmp.2015.09.004 ◽

2015 ◽

Vol 99 (3) ◽

pp. 485-491 ◽

Cited By ~ 29

Author(s):

Linea Melchior ◽

Morten Grauslund ◽

Beatriz Bellosillo ◽

Clara Montagut ◽

Erica Torres ◽

...

Keyword(s):

Malignant Melanoma ◽

Braf Mutation ◽

The Novel ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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MicroRNA Expression in Formalin-Fixed, Paraffin-Embedded Samples of Canine Cutaneous and Oral Melanoma by RT-qPCR

Veterinary Pathology ◽

10.1177/0300985819868646 ◽

2019 ◽

Vol 56 (6) ◽

pp. 848-855 ◽

Cited By ~ 1

Author(s):

Valentina Zamarian ◽

Carlotta Catozzi ◽

Lorenzo Ressel ◽

Riccardo Finotello ◽

Fabrizio Ceciliani ◽

...

Keyword(s):

Malignant Melanoma ◽

Signaling Pathways ◽

Noncoding Rna ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Ras Signaling ◽

Clinical Biomarkers ◽

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Formalin Fixed

MicroRNAs (miRNAs) are a class of small, noncoding RNA that post-transcriptionally regulate protein expression. miRNAs are emerging as clinical biomarkers of many diseases, including tumors. The aim of this study was to investigate whether miRNA expression could vary in melanoma samples derived from formalin-fixed, paraffin-embedded (FFPE) tissues. The study included 4 groups: (1) 9 samples of oral canine malignant melanoma, (2) 10 samples of cutaneous malignant melanoma, (3) 5 samples of healthy oral mucosa, and (4) 7 samples of healthy skin. The expression levels of 6 miRNAs—miR-145, miR-146a, miR-425-5p, miR-223, miR-365, and miR-134—were detected and assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR) using TaqMan probes. Cutaneous canine malignant melanoma showed a decrease of the expression level of miR-145 and miR-365 and an increase of miR-146a and miR-425-5p compared to control samples. MiR-145 was also downregulated in oral canine malignant melanoma. The miRNAs with decreased expression may regulate genes involved in RAS, Rap1, and transforming growth factor β (TGF-β) signaling pathways, as well as upregulated genes associated with phosphatidylinositol signaling system, adherens junction, and RAS signaling pathways. In conclusion, miR-145, miR-365, miR-146a, and miR-425-5p were differentially expressed in canine malignant melanoma and healthy FFPE samples, suggesting that they may play a role in canine malignant melanoma pathogenesis.

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A multisite blinded study for the detection of BRAF mutations in formalin-fixed, paraffin-embedded malignant melanoma

Scientific Reports ◽

10.1038/srep01659 ◽

2013 ◽

Vol 3 (1) ◽

Cited By ~ 26

Author(s):

Anna Richter ◽

Fabienne Grieu ◽

Amerigo Carrello ◽

Benhur Amanuel ◽

Kateh Namdarian ◽

...

Keyword(s):

Malignant Melanoma ◽

Braf Mutations ◽

Formalin Fixed Paraffin ◽

Blinded Study ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Cutaneous Sarcoids in Captive African Lions Associated With Feline Sarcoid-Associated Papillomavirus Infection

Veterinary Pathology ◽

10.1177/0300985810391111 ◽

2010 ◽

Vol 48 (6) ◽

pp. 1176-1179 ◽

Cited By ~ 19

Author(s):

G. M. B. Orbell ◽

S. Young ◽

J. S. Munday

Keyword(s):

Dna Sequences ◽

Skin Lesions ◽

Cutaneous Lesions ◽

Clinically Normal ◽

Papillomavirus Infection ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Polymerase Chain ◽

African Lions ◽

Formalin Fixed

Solitary and multiple cutaneous and mucocutaneous masses were identified in 5 of 24 captive African lions ( Panthera leo) over a 6-month-period. All masses were surgically excised, and all were histologically similar to equine and feline sarcoids. DNA was extracted from formalin-fixed, paraffin-embedded tissue. Polymerase chain reaction amplified DNA sequences that had been previously detected in feline sarcoids and clinically normal bovine skin. All lions had been fed a diet that included bovine carcasses that had not been skinned. Since the cessation of feeding bovine carcasses with cutaneous lesions, no additional skin lesions have been observed within any of the lions. Herein is described the clinical, gross, and histopathological findings of sarcoids in 5 captive lions. As the causative papillomavirus most likely has a bovine definitive host, it is hypothesized that the lions were exposed to the virus by feeding on bovine carcasses with skin still attached.

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