scholarly journals CD4+CD25+ T Regulatory Cells, Immunotherapy of Cancer, and Interleukin-2

2005 ◽  
Vol 28 (2) ◽  
pp. 120-128 ◽  
Author(s):  
Paul Andrew Antony ◽  
Nicholas P Restifo
Keyword(s):  
T Regulatory Cells ◽  
Interleukin 2 ◽  
Regulatory Cells ◽  
Immunotherapy Of Cancer

Immune Suppression by Placenta Derived Adherent Cells (PDAC) Correlate with Monocyte Chemoattractant Protein-1 and IL-2 Secretion.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3883-3883
Author(s):  
Casper Paludan ◽  
Ryhor Harbacheuski ◽  
Rose Ann Murray ◽  
Megan Mendillo ◽  
Jorge Soler ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Immune Suppression ◽  
T Regulatory Cells ◽  
Interleukin 2 ◽  
Adherent Cells ◽  
Regulatory Cells ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein

Abstract The placenta is a readily available and ethically non-controversial source of large amounts of therapeutic stem cells. Placenta Derived Adherent Cells (PDACs) are isolated from the placenta by one of several methods including physical disruption of tissue from several different anatomical sites within the placenta that include the amniotic membrane, chorion, placental cotyledons, or any combination thereof. Flow cytometry analysis showed that PDACs isolated from certain sites exhibit defined phenotypes, including for example CD200+ CD105+ CD73+ CD34− CD45− at percentages ≥70% and constitutively secrete IL-6, IL-8, and Monocyte Chemoattractant Protein-1 (MCP-1). PDACs demonstrate in vitro pluripotency in the adipogenic, osteogenic, and chondrogenic lineages. Furthermore, PDACs suppress T cell proliferation in certain Mixed Leukocyte Reaction (MLR) and the autologous EBV regression assays. Because secreted factors can powerfully modify immune responses and influence therapeutic use of cells, we report on the cytokine secretion in certain PDAC MLR and regression assays. Cytokines were measured on a Luminex system in supernatants from 6-day PDAC cultures, PDAC MLRs or PDAC regression assays. MLRs include PDACs, Dendritic Cells (DC)s, and T cells at DC/PDAC/T ratios 1/2/10. EBV regression assays included PDACs, EBV antigen-presenting cells (APC), and T cells at APC/PDAC/T ratios 1/2/10. Levels of IL-6 (11 ng/ml) and IL-8 (16 ng/ml) stayed constant in PDAC solo cultures, PDAC MLRs, and PDAC regression assays. MCP-1 concentration was 2 ng/ml in PDAC solo cultures, and non-suppressive control adherent cell MLRs and regression assays, but increased to 10 ng/ml in suppressed PDAC MLRs and PDAC regression assays. These values are consistent with reported MCP-1 serum levels. Interleukin-2 (IL-2) is both a T cell survival factor and an obligate factor for CD4+CD25+ T regulatory cells. T regulatory cells are not required for PDAC T cell suppression, but IL-2 levels consistently increase when MLR suppression by PDACs occurs. The CD4 MLR supernatants contained 65 pg/ml IL-2, and the CD8 MLR contained 35 pg/ml IL-2. In the 85% and 75% suppressed CD4 and CD8 PDAC MLRs, the IL-2 levels rose 5-fold to 331 pg/ml (CD4) and 2-fold to 67 pg/ml(CD8). These results indicate that IL-2 and MCP-1, traditionally known as stimulators of the immune response, may play a role in PDAC immune suppression. PDACs, which cause the secretion, may thus be useful therapeutic tools in the clinic.

scholarly journals Effects of Low-Dose Recombinant Interleukin 2 to Promote T-Regulatory Cells in Alopecia Areata

2014 ◽  
Vol 150 (7) ◽  
pp. 748 ◽  
Author(s):  
Emeline Castela ◽  
Florence Le Duff ◽  
Catherine Butori ◽  
Michel Ticchioni ◽  
Paul Hofman ◽  
...  
Keyword(s):  
Alopecia Areata ◽  
Low Dose ◽  
T Regulatory Cells ◽  
Interleukin 2 ◽  
Regulatory Cells ◽  
Recombinant Interleukin 2

scholarly journals CD4+ CD25+ Foxp3− T-Regulatory Cells Produce both Gamma Interferon and Interleukin-10 during Acute Severe Murine Spotted Fever Rickettsiosis

2009 ◽  
Vol 77 (9) ◽  
pp. 3838-3849 ◽  
Author(s):  
Rong Fang ◽  
Nahed Ismail ◽  
Thomas Shelite ◽  
David H. Walker
Keyword(s):  
T Cells ◽  
T Regulatory Cells ◽  
Spotted Fever ◽  
Bacterial Load ◽  
Interleukin 2 ◽  
Gamma Interferon ◽  
Spotted Fever Group ◽  
Regulatory Cells ◽  
Lethal Infection ◽  
Ifn Γ

ABSTRACT Spotted fever group rickettsiae cause life-threatening human infections worldwide. Until now, the immune regulatory mechanisms involved in fatal rickettsial infection have been unknown. C3H/HeN mice infected with 3 × 105 PFU of R ickettsia conorii developed an acute progressive disease, and all mice succumbed to this infection. A sublethal infection induced protective immunity, and mice survived. Compared to splenic T cells from sublethally infected mice, splenic T cells from lethally infected mice produced significantly lower levels of interleukin-2 (IL-2) and gamma interferon (IFN-γ) and a higher level of IL-10, but not of IL-4 or transforming growth factor β, and there was markedly suppressed CD4+ T-cell proliferation in response to antigen-specific stimulation with R. conorii. Furthermore, lethal infection induced significant expansion of CD4+ CD25+ Foxp3− T cells in infected organs compared to the levels in naïve and sublethally infected mice. In a lethal infection, splenic CD4+ CD25+ Foxp3− T cells, which were CTLA-4high T-bet+ and secreted both IFN-γ and IL-10, suppressed the proliferation of and IL-2 production by splenic CD4+ CD25− Foxp3− T cells in vitro. Interestingly, depletion of CD25+ T cells in vivo did not change the disease progression, but it increased the bacterial load in the lung and liver, significantly reduced the number of IFN-γ-producing Th1 cells in the spleen, and increased the serum levels of IFN-γ. These results suggested that CD4+ CD25+ T cells generated in acute murine spotted fever rickettsiosis are Th1-cell-related adaptive T-regulatory cells, which substantially contribute to suppressing the systemic immune response, possibly by a mechanism involving IL-10 and/or cytotoxic T-lymphocyte antigen 4.

Expansion of peripheral naturally occurring T regulatory cells by Fms-like tyrosine kinase 3 ligand treatment

Blood ◽  
2009 ◽  
Vol 113 (25) ◽  
pp. 6277-6287 ◽  
Author(s):  
Lee Kim Swee ◽  
Nabil Bosco ◽  
Bernard Malissen ◽  
Rhodri Ceredig ◽  
Antonius Rolink
Keyword(s):  
T Cell ◽  
Tyrosine Kinase ◽  
T Regulatory Cells ◽  
Interleukin 2 ◽  
Fold Increase ◽  
Cell Contact ◽  
Regulatory Cells ◽  
Independent Manner ◽  
Naturally Occurring

Abstract Fms-like tyrosine kinase 3 ligand (FLT3L) plays a major role in dendritic cell (DC) biology. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its availability (by repetitive injections for 7-10 days) leads to a 10-fold increase in DC numbers. In this study, we show that FLT3L treatment indirectly leads to an expansion of peripheral naturally occurring T regulatory cells (NTregs). The FLT3L-induced increase in NTregs was still observed in thymectomized mice, ruling out the role of the thymus in this mechanism. Instead, the increased number of NTregs was due to proliferation of preexisting NTregs, most likely due to favored interactions with increased number of DCs. In vitro, we show that DCs induce regulatory T-cell (Treg) proliferation by direct cell contact and in an interleukin-2–dependent, T-cell receptor–independent manner. FLT3L could prevent death induced by acute graft-versus-host disease (GVHD). This study demonstrates unique aspects in the regulation of Treg homeostasis by DCs, which were unappreciated until now. It also reinforces the relevance of FLT3L treatment in GVHD by its ability to increase both the number of tolerizing DCs and NTregs.

Recombinant cytokines in the practice of the therapist

2021 ◽  
pp. 11-18
Author(s):  
Margarita Valentinovna Antonenko
Keyword(s):  
Tissue Repair ◽  
T Regulatory Cells ◽  
Interleukin 2 ◽  
Premature Death ◽  
Immunological Tolerance ◽  
Regulatory Cells ◽  
Activated T Cells ◽  
Tissue Repair And Regeneration ◽  
Proliferation And Differentiation ◽  
Recombinant Interleukin 2

The article contains information presented in the open press based on the results of studies of the clinical efficacy of recombinant interleukin 2. Roncoleukin provides immune protection against tumor cells, pathogens of viral, bacterial and fungal nature, activates the processes of tissue repair and regeneration, promotes adequate interaction of the immune, endocrine and nervous systems. Interleukin-2 is an activation, proliferation and differentiation factor for T- and B-lymphocytes, T-regulatory cells, natural killer cells, monocytes / macrophages, dendritic and oligodendroglial cells; protects activated T cells from premature death (apoptosis) and cancels immunological tolerance.

Sign in / Sign up

Export Citation Format

Share Document