Molecular Portraits of Heterogeneity Related to Cancer Stem Cells in Human Ovarian Cancer

2014 ◽  
Vol 24 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Jianfang Zeng ◽  
Jie Ruan ◽  
Lijing Luo ◽  
Jie Shi ◽  
Quancai Cui ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Immunohistochemical Staining ◽  
Intratumoral Heterogeneity ◽  
Diagnosis And Treatment ◽  
Pairwise Comparisons ◽  
Human Ovarian Cancer ◽  
Ovarian Adenocarcinoma ◽  
Epithelial Cancers

PurposeThe aim of this study was to investigate molecular portraits of heterogeneity related to cancer stem cells (CSCs) in human ovarian cancer and to access the value in diagnosis and treatment.MethodsSixty specimens were collected in both cytoreductive and re-cytoreductive surgeries of 20 serous papillary ovarian adenocarcinoma cases. Expression density and distribution of 3 CSC markers (CD44, CD133, and CD117) and 3 stemness proteins (Bmi1, Nestin, and Oct3/4) were analyzed by immunohistochemical staining. Pairwise comparisons were performed among their expression in primary, metastasis, and relapsing tumors.ResultsSome molecules presented different localization in 1 tissue, like CD133 and CD117, and all but Oct3/4 expressed differentially in different specimens of 1 case. Compared to primary or metastatic cancers, recurrent cancers show higher expression of CD133, CD117, and Bmi1, as well as higher histological grades.ConclusionsOur study indicated that there exist extratumoral and intratumoral heterogeneity in ovarian epithelial cancers related to CSCs. And this is worth further studying.

scholarly journals Novel Nano-Therapeutic Approach Actively Targets Human Ovarian Cancer Stem Cells after Xenograft into Nude Mice

2017 ◽  
Vol 18 (4) ◽  
pp. 813 ◽  
Author(s):  
Amoura Abou-ElNaga ◽  
Ghada Mutawa ◽  
Ibrahim El-Sherbiny ◽  
Hassan Abd-ElGhaffar ◽  
Ahmed Allam ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Nude Mice ◽  
Therapeutic Approach ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Stem Cells

Influence of Active and Non-active Protein C on Human Ovarian Cancer Stem Cells

2012 ◽  
Vol 2012 (1) ◽  
pp. 55
Author(s):  
H Al Thawadi ◽  
S Mirshahi ◽  
H Al Farsi ◽  
D Azzazen ◽  
S Besbes ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Protein C ◽  
Human Ovarian Cancer ◽  
Active Protein ◽  
Ovarian Cancer Stem Cells

MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A

Gene ◽  
2017 ◽  
Vol 605 ◽  
pp. 99-107 ◽  
Author(s):  
Cui Chang ◽  
Te Liu ◽  
Yongyi Huang ◽  
Wenxing Qin ◽  
Hongtu Yang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Stem Cells ◽  
Potential Inhibitor

Superparamagnetic Iron Oxide Nanoparticles Induce Ferroptosis of Human Ovarian Cancer Stem Cells by Weakening Cellular Autophagy

2020 ◽  
Vol 16 (11) ◽  
pp. 1612-1622
Author(s):  
Yongyi Huang ◽  
Jiajia Lin ◽  
Ying Xiong ◽  
Juan Chen ◽  
Xiling Du ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ovarian Cancer ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Drug Resistance ◽  
Cancer Stem Cells ◽  
Superparamagnetic Iron Oxide ◽  
Oxide Nanoparticles ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Stem Cells

Human ovarian cancer stem cells (HuOCSCs) are the main source of ovarian cancer recurrence, metastasis, and drug resistance. Superparamagnetic iron oxide nanoparticles (SPIONs) are well-known nucleic acid or drug carriers owing to their controllable properties, superior stability, and easy modification. However, whether SPIONs can inhibit the activity of HuOCSCs by inducing ferroptosis remains unclear. In the present study, we isolated CD44+ /CD133+ HuOCSCs from tumours of four patients with clear cell ovarian cancer and added 0.2 mM SPIONs for mixed culture. Transmission electron microscopy showed that SPION-treated HuOCSCs contained multiple high-density electron clouds. Prussian blue staining showed high concentrations of iron ions in the cells. In vitro , SPIONs treatment of HuOCSCs inhibited cell proliferation, migration, and soft agar clone formation, weakened their resistance to multiple chemotherapeutics, and induced cell death. In vivo , SPIONs pretreatment of HuOCSCs significantly reduced their tumour-forming ability and induced angiogenesis in nude mice. Further, SPIONs induced the accumulation of reactive oxygen species in HuOCSCs and induced oxidative stress. qPCR analysis indicated that SPIONs-treated HuOCSCs had reduced expression of tumour stem cell markers (CD117, NANOG, CD133, and SOX2), cell proliferation factors (KI67, CCND), autophagy-related factors (ATG3, ATG5, MAP1ALC3a, MAP1ALC3b, and MAP1ALC3c), and certain negative regulators of ferroptosis, while the mRNA expression levels of cell death-related proteins (BAK1 and BID), and certain positive regulators of ferroptosis were significantly increased. Overall, our findings suggest that SPIONs induce oxidative stress and decrease autophagy activity in ovarian cancer stem cells, activate ferroptosis, and inhibit their proliferation, invasion, drug resistance, and tumorigenic ability.

scholarly journals Ectopic expression of the WWOX gene suppresses stemness of human ovarian cancer stem cells

2015 ◽  
Vol 9 (4) ◽  
pp. 1614-1620 ◽  
Author(s):  
HONG CHAO YAN ◽  
JUN XU ◽  
LI SHA FANG ◽  
YING YING QIU ◽  
XIAO MAN LIN ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Ectopic Expression ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Stem Cells ◽  
Wwox Gene

Abstract 3056: Human ovarian cancer stem cells: In vitro cultivation and characterization

2014 ◽  
Author(s):  
Tatsuya Ishiguro ◽  
Hirokazu Ohata ◽  
Hitoshi Nakagama ◽  
Koji Okamoto ◽  
Kenichi Tanaka ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
In Vitro Cultivation ◽  
Human Ovarian Cancer ◽  
Ovarian Cancer Stem Cells
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