scholarly journals Clusterin expression in nontumor tissue in patients with resectable hepatocellular carcinoma related with postresectional survival

2019 ◽  
Vol 82 (12) ◽  
pp. 929-934 ◽  
Author(s):  
Po-Chung Kuo ◽  
Ivy Yenwen Chau ◽  
Anna Fen-Yau Li ◽  
Yat-Pang Chau ◽  
Cheng-Yuan Hsia ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Nontumor Tissue ◽  
Clusterin Expression

scholarly journals Role of microRNA-210-3p in hepatitis B virus-related hepatocellular carcinoma

2020 ◽  
Vol 318 (3) ◽  
pp. G401-G409
Author(s):  
Asahiro Morishita ◽  
Koji Fujita ◽  
Hisakazu Iwama ◽  
Taiga Chiyo ◽  
Shintaro Fujihara ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Target Genes ◽  
Aberrant Expression ◽  
Tissue Samples ◽  
Nontumor Tissue ◽  
B Virus ◽  
Enhanced Expression ◽  
Hcc Cells

Hepatitis B virus (HBV)-related hepatocarcinogenesis is not necessarily associated with the liver fibrotic stage and is occasionally seen at early fibrotic stages. MicroRNAs (miRNAs) are essentially 18- to 22-nucleotide-long endogenous noncoding RNAs. Aberrant miRNA expression is a common feature of various human cancers. The aberrant expression of specific miRNAs has been shown in hepatocellular carcinoma (HCC) tissue compared with nontumor tissue. Thus, we examined targetable miRNAs as a potential new biomarker related to the high risk of HBV-related hepatocarcinogenesis, toward the prevention of cancer-related deaths. HCC tissue samples from 29 patients who underwent hepatectomy at our hospital in 2002–2013 were obtained. We extracted the total RNA and analyzed it by microRNA array, real-time RT-PCR, and three comparisons: 1) HBV-related HCC and adjacent nontumor tissue, 2) HCV-related HCC and adjacent nontumor tissue, and 3) non-HBV-, non-HCV-related HCC and adjacent nontumor tissue. We also performed a functional analysis of miRNAs specific for HBV-related HCC by using HBV-positive HCC cell lines. MiR-210-3p expression was significantly increased only in the HBV-related HCC tissue samples. MiR-210-3p expression was upregulated, and the levels of its target genes were reduced in the HBV-positive HCC cells. The inhibition of miR-210-3p enhanced its target gene expression in the HBV-positive HCC cells. In addition, miR-210-3p regulated the HBx expression in HBV-infected Huh7/NTCP cells. The enhanced expression of miR-210-3p was detected specifically in HBV-related HCC and regulated various target genes, including HBx in the HBV-positive HCC cells. MiR-210-3p might, thus, be a new biomarker for the risk of HBV-related HCC. NEW & NOTEWORTHY Our present study demonstrated that miR-210-3p is the only microRNA with enhanced expression in HBV-related HCC, and the enhanced expression of miR-210-3p upregulates HBx expression. Therefore, miR-210-3p might be a pivotal biomarker of HBV-related hepatocarcinogenesis, and the inhibition of miR-210-3p could prevent inducing hepatocarcinogenesis related to HBV infection.

Clusterin expression in cholestasis, hepatocellular carcinoma and liver fibrosis

2009 ◽  
Vol 54 (5) ◽  
pp. 561-570 ◽  
Author(s):  
Ariane Aigelsreiter ◽  
Elke Janig ◽  
Julia Sostaric ◽  
Martin Pichler ◽  
Daniela Unterthor ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Fibrosis ◽  
Clusterin Expression

scholarly journals Evidence of Neutral Evolution of Mitochondrial DNA in Human Hepatocellular Carcinoma

2019 ◽  
Vol 11 (10) ◽  
pp. 2909-2916 ◽  
Author(s):  
Qi Liu ◽  
Deng Lin ◽  
Mingkun Li ◽  
Zhenglong Gu ◽  
Yiqiang Zhao
Keyword(s):  
Hepatocellular Carcinoma ◽  
Mitochondrial Dna ◽  
Nuclear Genome ◽  
Purifying Selection ◽  
Neutral Evolution ◽  
Single Nucleotide Variants ◽  
Copy Numbers ◽  
Nontumor Tissue ◽  
Mtdna Diversity ◽  
Single Tumor

Abstract Many studies have suggested that mitochondria and mitochondrial DNA (mtDNA) might be functionally associated with tumor genesis and development. Although the heterogeneity of tumors is well known, most studies were based on the analysis of a single tumor sample. The extent of mtDNA diversity in the same tumor is unclear, as is whether the diversity is influenced by selection pressure. Here, we analyzed the whole exon data from 1 nontumor sample and 23 tumor samples from different locations of one single tumor tissue from a hepatocellular carcinoma (HCC) patient. Among 18 heteroplasmic sites identified in the tumor, only 2 heteroplasmies were shared among all tumor samples. By investigating the correlations between the occurrence and frequency of heteroplasmy (Het) and sampling locations (Coordinate), relative mitochondrial copy numbers, and single-nucleotide variants in the nuclear genome, we found that the Coordinate was significantly correlated with Het, suggesting no strong purifying selection or positive selection acted on the mtDNA in HCC. By further investigating the allele frequency and proportion of nonsynonymous mutations in the tumor mtDNA, we found that mtDNA in HCC did not undergo extra selection compared with mtDNA in the adjacent nontumor tissue, and they both likely evolved under neutral selection.

HBV-DNA sequences in tumor and nontumor tissue in a patient with the fibrolamellar variant of hepatocellular carcinoma

Hepatology ◽  
1990 ◽  
Vol 12 (4) ◽  
pp. 676-679 ◽  
Author(s):  
Fergus D. Davison ◽  
Elizabeth A. Fagan ◽  
Bernard Portmann ◽  
Roger Williams
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Sequences ◽  
Hbv Dna ◽  
Nontumor Tissue

Overexpression of PSF1 is correlated with poor prognosis in hepatocellular carcinoma patients

2015 ◽  
Vol 30 (1) ◽  
pp. 56-64 ◽  
Author(s):  
Lei Zhou ◽  
Xue-jun Sun ◽  
Chang Liu ◽  
Qi-fei Wu ◽  
Ming-hui Tai ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Normal Liver ◽  
Down Regulation ◽  
New Therapeutic Approaches ◽  
Nontumor Tissue ◽  
Close Relationship ◽  
Cell Invasiveness ◽  
Interfering Rna

Background PSF1 is a subunit of the GINS complex which is essential for establishment of DNA replication forks, and the progression of the replisome. Previous studies have shown a close relationship between PSF1 and cell cycle in the proliferation of immature cells as well as tumors. The purpose of this study was to measure PSF1 expression in hepatocellular carcinoma (HCC) tissues, and determine the effects of down-regulation of PSF1 expression on growth of cancer cells, the cell cycle, apoptosis and cell invasiveness. Methods Samples from 137 HCC tissues, 67 from adjacent nontumor tissue and 15 from normal liver were studied using immunochemistry. The HepG2 cell line was used for knockdown experiments studied by RT-PCR, real-time PCR, apoptosis and invasiveness assays. Results PSF1 was overexpressed in HCC tissues compared with normal liver tissues. High PSF1 expression correlated with a more aggressive phenotype as well as worse prognosis in HCC patients. Knockdown of PSF1 expression using small interfering RNA (siRNA) slowed the growth of cancer cell by suppressing the cell cycle progression as well as increasing apoptosis, especially early apoptosis. In addition, the invasiveness of HepG2 cells was also reduced by down-regulation of PSF1. Conclusions These results suggest that the inhibition of PSF1 might provide new therapeutic approaches for HCC.

Diagnostic and prognostic significance of secretory clusterin expression in patients with hepatocellular carcinoma

Tumor Biology ◽  
2015 ◽  
Vol 37 (1) ◽  
pp. 999-1008 ◽  
Author(s):  
Wenjie Zheng ◽  
Min Yao ◽  
Wenli Sai ◽  
Qi Qian ◽  
Liuhong Pan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Prognostic Significance ◽  
Clusterin Expression

Study on Diagnostic Values of Astrocyte Elevated Gene 1 (AEG-1) and Glypican 3 (GPC-3) in Hepatocellular Carcinoma

2019 ◽  
Vol 152 (5) ◽  
pp. 647-655 ◽  
Author(s):  
Wenqing Cao ◽  
Meenal Sharma ◽  
Rami Imam ◽  
Jiangzhou Yu
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Sensitivity ◽  
High Specificity ◽  
The Other ◽  
Diagnostic Potential ◽  
Dysplastic Nodules ◽  
Nontumor Tissue ◽  
Diagnostic Values ◽  
Low Sensitivity ◽  
Sensitivity Specificity

Abstract Objectives To investigate the diagnostic potential of AEG-1 and GPC-3 in hepatocellular carcinoma (HCC). Methods AEG-1 and GPC-3 immunohistochemistry were performed on HCC, adjacent nontumor tissue (ANT), and dysplastic nodules (DN). Results H score of AEG-1 or GPC-3 in HCC was significantly higher than in ANT or DN. In HCC, 92% and 54% showed AEG-1 and GPC-3 positivity, respectively. In ANT, 16.2% were AEG-1 and 7.6% GPC-3 positive. AEG-1 staining was mostly diffuse, whereas GPC-3 frequently showed focal staining. AEG-1 alone showed high sensitivity but low specificity and accuracy. GPC-3, on the other hand, showed high specificity but low sensitivity and accuracy. Combination of both stains boosted the sensitivity, specificity, and accuracy to 94.6%, 89.5%, and 90.5%, respectively, when only diffuse staining was considered as positive. Conclusions AEG-1 or GPC-3 alone seemed not an ideal marker for HCC. The combination of AEG-1 and GPC-3 might improve early diagnosis of HCC.

scholarly journals Evidence of Hepatitis B Virus Infection in Cancer and Noncancer Stem Cells Associated with Human Hepatocellular Carcinoma

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Gerald Y. Minuk ◽  
Wendy Bautista ◽  
Julianne Klein
Keyword(s):  
Hepatocellular Carcinoma ◽  
Stem Cells ◽  
Hepatitis B Virus ◽  
Cancer Stem Cells ◽  
Virus Infection ◽  
Hepatitis B ◽  
Hepatitis B Virus Infection ◽  
Nontumor Tissue ◽  
B Virus ◽  
Chronic Hbv

Both the hepatitis B virus (HBV) and cancer stem cells (CSCs) have been independently implicated in the pathogenesis of hepatocellular carcinoma (HCC). To date, there have been no reports describing HBV infection within CSCs. In this report we describe HBV core (HBcAg) and HBx protein expression within CSCs associated with human HCC. HBV markers were also identified in nonmalignant stem cells present in adjacent nontumor tissue. These findings provide new insights into the pathogenesis of HBV-induced HCC and are potentially relevant to the treatment of both HCC and chronic HBV.

Morphology of tumor and nontumor tissue in liver resection specimens for hepatocellular carcinoma following nivolumab therapy

2020 ◽  
Author(s):  
Camila C. Simoes ◽  
Swan N. Thung ◽  
Maria Isabel Fiel ◽  
Max W. Sung ◽  
Myron E. Schwartz ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Resection ◽  
Nontumor Tissue
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