scholarly journals Cell Cycle Kinetic Analysis of Colorectal Neoplasms Using a New Automated Immunohistochemistry-Based Cell Cycle Detection Method

Medicine ◽  
2015 ◽  
Vol 94 (4) ◽  
pp. e501 ◽  
Author(s):  
Ayako Tomono ◽  
Tomoo Itoh ◽  
Emmy Yanagita ◽  
Naoko Imagawa ◽  
Yoshihiro Kakeji
Keyword(s):  
Cell Cycle ◽  
Kinetic Analysis ◽  
Colorectal Neoplasms ◽  
Detection Method ◽  
Cell Cycle Kinetic ◽  
Cycle Detection

Cell cycle kinetic analysis with flow cytometry in pea root meristem synchronized with aphidicolin

1991 ◽  
Vol 81 (4) ◽  
pp. 507-512 ◽  
Author(s):  
Sergio Sgorbati ◽  
Elio Sparvoli ◽  
Marisa Levi ◽  
Maria Grazia Galli ◽  
Sandra Citterio ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Kinetic Analysis ◽  
Root Meristem ◽  
Cell Cycle Kinetic ◽  
Pea Root

Cell cycle kinetic analysis with flow cytometry in pea root meristem synchronized with aphidicolin

1991 ◽  
Vol 81 (4) ◽  
pp. 507-512 ◽  
Author(s):  
Sergio Sgorbati ◽  
Elio Sparvoli ◽  
Marisa Levi ◽  
Maria Grazia Galli ◽  
Sandra Citterio ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Flow Cytometry ◽  
Kinetic Analysis ◽  
Root Meristem ◽  
Cell Cycle Kinetic ◽  
Pea Root

scholarly journals Inhibition of vitamin D analog eldecalcitol on hepatoma in vitro and in vivo

Open Medicine ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. 663-671
Author(s):  
Limin Ye ◽  
Liyi Zhu ◽  
Jinglin Wang ◽  
Fei Li
Keyword(s):  
Cell Cycle ◽  
Vitamin D ◽  
Cell Apoptosis ◽  
Control Group ◽  
High Morbidity ◽  
Rt Pcr ◽  
Vitamin D Analog ◽  
E Cadherin ◽  
Cycle Detection

AbstractHepatoma is a serious liver cancer with high morbidity and mortality. Eldecalcitol (ED-71), a vitamin D analog, is extensively used as anti-cancer agent in vitro. Hepatocellular carcinoma cell, SMMC-7721 cell lines were used in this study. Transwell assay, cell apoptosis and cell cycle detection assays were investigated after treatment with ED-71 and phosphate buffered saline (PBS) as control. Sizes of tumors were measured after ED-71 treatment in a mouse model. E-cadherin and Akt gene expressions were detected by real-time PCR (RT-PCR). The results showed that cell invasion and migration were decreased markedly after ED-71 treatment compared to control group. Cell cycle detection showed that the G2 stage was 13.18% and total S-stage was 41.16% in the ED-71 group and G2 stage: 22.88%, total S-stage: 27.34% in the control group. Cell apoptosis rate was promoted in the ED-71 group. Size of the tumors reduced more after the ED-71 treatment than the PBS treatment in mice. ED-71 markedly inhibited the expression of Akt and E-cadherin, either detected by immunohistochemistry or RT-PCR. ED-71 treatment can inhibit the hepatoma agent proliferation by increasing the E-cadherin expression and decreasing Akt expression. Therefore, these findings provide novel evidence that ED-71 can be used as an anti-hepatoma agent.

scholarly journals Method for kinetic analysis of drug-induced cell cycle perturbations

Cytometry ◽  
1991 ◽  
Vol 12 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Paolo Ubezio ◽  
Stefania Filippeschi ◽  
Laura Spinelli
Keyword(s):  
Cell Cycle ◽  
Kinetic Analysis ◽  
Drug Induced

Cell Cycle Kinetic Effects of Tamoxifen on Human Breast Cancer Cells.

1993 ◽  
Vol 698 (1 Breast Cancer) ◽  
pp. 174-181 ◽  
Author(s):  
MARCO DANOVA ◽  
CARLO PELLICCIARI ◽  
CARLO ZIBERA ◽  
ROSANNA MANGIAROTTI ◽  
NADIA GIBELLI ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Cell Cycle Kinetic ◽  
Kinetic Effects

Immunohistochemistry-based Cell Cycle Detection (iCCD)

2012 ◽  
Vol 36 (5) ◽  
pp. 769-773 ◽  
Author(s):  
Emmy Yanagita ◽  
Shingo Kamoshida ◽  
Naoko Imagawa ◽  
Tomoo Itoh
Keyword(s):  
Cell Cycle ◽  
Cycle Detection
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