scholarly journals Stem cell bioprocessing: fundamentals and principles

2008 ◽  
Vol 6 (32) ◽  
pp. 209-232 ◽  
Author(s):  
Mark R Placzek ◽  
I-Ming Chung ◽  
Hugo M Macedo ◽  
Siti Ismail ◽  
Teresa Mortera Blanco ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Stem Cell Research ◽  
Adult Stem Cells ◽  
Organ Transplant ◽  
Clinical Applications ◽  
Cell Tissue ◽  
Fundamental Research

In recent years, the potential of stem cell research for tissue engineering-based therapies and regenerative medicine clinical applications has become well established. In 2006, Chung pioneered the first entire organ transplant using adult stem cells and a scaffold for clinical evaluation. With this a new milestone was achieved, with seven patients with myelomeningocele receiving stem cell-derived bladder transplants resulting in substantial improvements in their quality of life. While a bladder is a relatively simple organ, the breakthrough highlights the incredible benefits that can be gained from the cross-disciplinary nature of tissue engineering and regenerative medicine (TERM) that encompasses stem cell research and stem cell bioprocessing. Unquestionably, the development of bioprocess technologies for the transfer of the current laboratory-based practice of stem cell tissue culture to the clinic as therapeutics necessitates the application of engineering principles and practices to achieve control, reproducibility, automation, validation and safety of the process and the product. The successful translation will require contributions from fundamental research (from developmental biology to the ‘omics’ technologies and advances in immunology) and from existing industrial practice (biologics), especially on automation, quality assurance and regulation. The timely development, integration and execution of various components will be critical—failures of the past (such as in the commercialization of skin equivalents) on marketing, pricing, production and advertising should not be repeated. This review aims to address the principles required for successful stem cell bioprocessing so that they can be applied deftly to clinical applications.

scholarly journals Stem Cell Based Tissue Engineering and Regenerative Medicine: A Review Focusing on Adult Stem Cells

2012 ◽  
Vol 1 (1) ◽  
pp. 75-82
Author(s):  
Jordan Greenberg ◽  
Veronica Fortino ◽  
Daniel Pelaez ◽  
Herman S. Cheung
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Adult Stem Cells

scholarly journals Sources and Clinical Applications of Mesenchymal Stem Cells: State-of-the-art review

2018 ◽  
Vol 18 (3) ◽  
pp. 264 ◽  
Author(s):  
Roberto Berebichez-Fridman ◽  
Pablo R. Montero-Olvera
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Adult Stem Cells ◽  
Current Knowledge ◽  
Clinical Applications ◽  
The Body ◽  
Differentiation Potential ◽  
Advantages And Disadvantages

First discovered by Friedenstein in 1976, mesenchymal stem cells (MSCs) are adult stem cells found throughout the body that share a fixed set of characteristics. Discovered initially in the bone marrow, this cell source is considered the gold standard for clinical research, although various other sources—including adipose tissue, dental pulp, mobilised peripheral blood and birth-derived tissues—have since been identified. Although similar, MSCs derived from different sources possess distinct characteristics, advantages and disadvantages, including their differentiation potential and proliferation capacity, which influence their applicability. Hence, they may be used for specific clinical applications in the fields of regenerative medicine and tissue engineering. This review article summarises current knowledge regarding the various sources, characteristics and therapeutic applications of MSCs.Keywords: Mesenchymal Stem Cells; Adult Stem Cells; Regenerative Medicine; Cell Differentiation; Tissue Engineering.

Exploring and Exploiting Tissue Engineering Through the Design of Multifunctional Therapeutic Systems

2019 ◽  
Vol 14 (2) ◽  
pp. 80-82 ◽  
Author(s):  
Masoud Mozafari
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Stem Cell Research ◽  
Therapeutic Strategies ◽  
Common Theme ◽  
Special Issue ◽  
The Common ◽  
Lead Article

This article presents a special issue of "Current Stem Cell Research & Therapy" devoted to exploring and exploiting tissue engineering through the design of multifunctional therapeutic systems. This lead article draws from twelve contributed articles to discuss the most recent advancements in this emerging field. The common theme in the contributed articles is the emerging therapeutic strategies, and a special appeal is made for collaboration between engineers and biologists for the development of multifunctional therapeutic systems for tissue engineering and regenerative medicine.

scholarly journals Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications

2021 ◽  
Vol 8 (5) ◽  
pp. 68
Author(s):  
Diogo E.S. Nogueira ◽  
Joaquim M.S. Cabral ◽  
Carlos A.V. Rodrigues
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Large Scale ◽  
Adult Stem Cells ◽  
Regulatory Agencies ◽  
Human Stem Cells ◽  
Stem Cell Expansion ◽  
Single Use

Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications.

scholarly journals Nanotechnology meets regenerative medicine: a new frontier?

2013 ◽  
Vol 2 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Kenneth K.Y. Wong ◽  
Xuelai Liu
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Regenerative Medicine ◽  
Biological Properties ◽  
Clinical Applications ◽  
New Techniques ◽  
Cell Recruitment ◽  
Cellular Events ◽  
New Frontier ◽  
Cell Migration And Proliferation

AbstractRegenerative medicine is the creation of a tissue or organ with normal structures and functions to replace the lost or impaired ones via biological modulation and tissue engineering. Indeed, many researchers have focused on exploring new techniques or approaches in this field. In recent years, numerous nanotechnologies have been incorporated into the field of regenerative medicine, aiming to replace tissues. The application of nanotechnology is important as many nanomaterials exhibit novel biological properties in the modulation of cellular events. The two main directions in this field are to provide biologically compatible scaffolds as an optimal environment for cell migration and proliferation; as well as to attempt to induce stem cell recruitment and differentiation. Thus, this review will focus on these two aspects, briefly describing the clinical applications.

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