scholarly journals X-ray Free Electron Laser Determination of Crystal Structures of Dark and Light States of a Reversibly Photoswitching Fluorescent Protein at Room Temperature

2017 ◽  
Vol 18 (9) ◽  
pp. 1918 ◽  
Author(s):  
Christopher Hutchison ◽  
Violeta Cordon-Preciado ◽  
Rhodri Morgan ◽  
Takanori Nakane ◽  
Josie Ferreira ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Free Electron ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Fluorescent Protein ◽  
X Ray

Determination of x-ray free electron laser power using a room-temperature calorimeter

Metrologia ◽  
2016 ◽  
Vol 53 (1) ◽  
pp. 98-102 ◽  
Author(s):  
T Tanaka ◽  
M Kato ◽  
N Saito ◽  
K Tono ◽  
M Yabashi ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Laser Power ◽  
Room Temperature ◽  
X Ray

scholarly journals Liquid sample delivery techniques for serial femtosecond crystallography

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130337 ◽  
Author(s):  
Uwe Weierstall
Keyword(s):  
Flow Rate ◽  
Free Electron ◽  
Repetition Rate ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Liquid Flow Rate ◽  
Free Electron Lasers ◽  
Liquid Sample ◽  
X Ray ◽  
Serial Femtosecond Crystallography

X-ray free-electron lasers overcome the problem of radiation damage in protein crystallography and allow structure determination from micro- and nanocrystals at room temperature. To ensure that consecutive X-ray pulses do not probe previously exposed crystals, the sample needs to be replaced with the X-ray repetition rate, which ranges from 120 Hz at warm linac-based free-electron lasers to 1 MHz at superconducting linacs. Liquid injectors are therefore an essential part of a serial femtosecond crystallography experiment at an X-ray free-electron laser. Here, we compare different techniques of injecting microcrystals in solution into the pulsed X-ray beam in vacuum. Sample waste due to mismatch of the liquid flow rate to the X-ray repetition rate can be addressed through various techniques.

scholarly journals Structure determination of molecules in an alignment laser field by femtosecond photoelectron diffraction using an X-ray free-electron laser

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shinichirou Minemoto ◽  
Takahiro Teramoto ◽  
Hiroshi Akagi ◽  
Takashi Fujikawa ◽  
Takuya Majima ◽  
...  
Keyword(s):  
Structure Determination ◽  
Free Electron ◽  
Free Electron Laser ◽  
Laser Field ◽  
X Ray ◽  
Photoelectron Diffraction

scholarly journals Inline spectrometer for shot-by-shot determination of pulse energies of a two-color X-ray free-electron laser

2016 ◽  
Vol 23 (1) ◽  
pp. 331-333 ◽  
Author(s):  
Kenji Tamasaku ◽  
Yuichi Inubushi ◽  
Ichiro Inoue ◽  
Kensuke Tono ◽  
Makina Yabashi ◽  
...  
Keyword(s):  
Thin Film ◽  
Negative Correlation ◽  
Pulse Energy ◽  
Free Electron ◽  
Free Electron Laser ◽  
X Ray ◽  
The Absolute ◽  
Pulse Energies

An inline spectrometer has been developed to monitor shot-by-shot pulse energies of a two-color X-ray beam. A thin film of diamond allows inline operation with minimum absorption. The absolute pulse energy for each color is determined by the inline spectrometer combined with a total pulse-energy monitor. A negative correlation is found between the two-color pulse energies.

scholarly journals The Mn 4 Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser

2014 ◽  
Vol 369 (1647) ◽  
pp. 20130324 ◽  
Author(s):  
Rosalie Tran ◽  
Jan Kern ◽  
Johan Hattne ◽  
Sergey Koroidov ◽  
Julia Hellmich ◽  
...  
Keyword(s):  
Free Electron ◽  
Water Oxidation ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Oxidation Catalyst ◽  
Cryogenic Temperatures ◽  
New Approach ◽  
X Ray ◽  
The Past ◽  
Photosynthetic Water Oxidation

The structure of photosystem II and the catalytic intermediate states of the Mn 4 CaO 5 cluster involved in water oxidation have been studied intensively over the past several years. An understanding of the sequential chemistry of light absorption and the mechanism of water oxidation, however, requires a new approach beyond the conventional steady-state crystallography and X-ray spectroscopy at cryogenic temperatures. In this report, we present the preliminary progress using an X-ray free-electron laser to determine simultaneously the light-induced protein dynamics via crystallography and the local chemistry that occurs at the catalytic centre using X-ray spectroscopy under functional conditions at room temperature.

scholarly journals Co-crystal structure of the Fusobacterium ulcerans ZTP riboswitch using an X-ray free-electron laser

2019 ◽  
Vol 75 (7) ◽  
pp. 496-500
Author(s):  
Christopher Jones ◽  
Brandon Tran ◽  
Chelsie Conrad ◽  
Jason Stagno ◽  
Robert Trachman ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Small Molecules ◽  
Free Electron ◽  
Free Electron Laser ◽  
Room Temperature ◽  
Temperature Structure ◽  
Data Set ◽  
Time Resolved ◽  
Regulate Gene Expression ◽  
X Ray

Riboswitches are conformationally dynamic RNAs that regulate gene expression by binding specific small molecules. ZTP riboswitches bind the purine-biosynthetic intermediate 5-aminoimidazole-4-carboxamide riboside 5′-monophosphate (ZMP) and its triphosphorylated form (ZTP). Ligand binding to this riboswitch ultimately upregulates genes involved in folate and purine metabolism. Using an X-ray free-electron laser (XFEL), the room-temperature structure of the Fusobacterium ulcerans ZTP riboswitch bound to ZMP has now been determined at 4.1 Å resolution. This model, which was refined against a data set from ∼750 diffraction images (each from a single crystal), was found to be consistent with that previously obtained from data collected at 100 K using conventional synchrotron X-radiation. These experiments demonstrate the feasibility of time-resolved XFEL experiments to understand how the ZTP riboswitch accommodates cognate ligand binding.

Determination of X-ray pulse duration via intensity correlation measurements of X-ray fluorescence

2019 ◽  
Vol 26 (6) ◽  
pp. 2050-2054 ◽  
Author(s):  
Ichiro Inoue ◽  
Kenji Tamasaku ◽  
Taito Osaka ◽  
Yuichi Inubushi ◽  
Makina Yabashi
Keyword(s):  
Pulse Duration ◽  
Free Electron ◽  
Free Electron Laser ◽  
Coherence Time ◽  
Metal Foil ◽  
Intensity Correlation ◽  
Simple Method ◽  
X Ray

A simple method using X-ray fluorescence is proposed to diagnose the duration of an X-ray free-electron laser (XFEL) pulse. This work shows that the degree of intensity correlation of the X-ray fluorescence generated by irradiating an XFEL pulse on metal foil reflects the magnitude relation between the XFEL duration and the coherence time of the fluorescence. Through intensity correlation measurements of copper Kα fluorescence, the duration of 12 keV XFEL pulses from SACLA was evaluated to be ∼10 fs.

Determination of the Pulse Duration of an X-Ray Free Electron Laser Using Highly Resolved Single-Shot Spectra

2012 ◽  
Vol 109 (14) ◽  
Author(s):  
Yuichi Inubushi ◽  
Kensuke Tono ◽  
Tadashi Togashi ◽  
Takahiro Sato ◽  
Takaki Hatsui ◽  
...  
Keyword(s):  
Pulse Duration ◽  
Free Electron ◽  
Free Electron Laser ◽  
Single Shot ◽  
X Ray
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