scholarly journals The Use of Protein A, from Staphylococcus aureus, in Immune Electron Microscopy for Detecting Plant Virus Particles

1979 ◽  
Vol 45 (2) ◽  
pp. 533-536 ◽  
Author(s):  
D. D. Shukla ◽  
K. H. Gough
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Plant Virus ◽  
Protein A ◽  
Immune Electron Microscopy ◽  
Virus Particles

Search mode for rapid detection of virus particles

1991 ◽  
Vol 49 ◽  
pp. 286-287
Author(s):  
O. E. Bradfute
Keyword(s):  
Electron Microscopy ◽  
Rapid Detection ◽  
Plant Virus ◽  
Low Dose ◽  
Search Mode ◽  
Virus Diseases ◽  
Virus Particles ◽  
Focus Image ◽  
Time Required ◽  
Rapid Switching

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.

scholarly journals Cell Wall-associated Protein A as a Tool for Immunolocalization of Staphylococcus aureus in Infected Human Airway Epithelium

2000 ◽  
Vol 48 (4) ◽  
pp. 523-533 ◽  
Author(s):  
Emmanuel Mongodin ◽  
Odile Bajolet ◽  
Jocelyne Hinnrasky ◽  
Edith Puchelle ◽  
Sophie de Bentzmann
Keyword(s):  
Electron Microscopy ◽  
Staphylococcus Aureus ◽  
Epithelial Cells ◽  
Airway Epithelium ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Basal Cells ◽  
Bronchial Diseases

Staphylococcus aureus is a common human pathogen involved in non-bronchial diseases and in genetic and acquired bronchial diseases. In this study, we applied an immunolabeling approach for in vivo and in vitro detection of S. aureus, based on the affinity of staphylococcal protein A (SpA) for the Fc region of immunoglobulins, especially IgG. Most strains of S. aureus, including clinical strains, can be detected with this labeling technique. The approach can be used for detection and localization with transmission electron microscopy or light-fluorescence microscopy of S. aureus in infected tissues such as human bronchial tissue from cystic fibrosis (CF) patients. The methodology can also be applied to cell culture models with the aim of characterizing bacterial adherence to epithelial cells in backscattered electron imaging with scanning electron microscopy. Application to the study of S. aureus adherence to airway epithelium showed that the bacteria did not adhere in vivo to intact airway epithelium. In contrast, bacteria adhered to the basolateral plasma membrane of columnar cells, to basal cells, to the basement membrane and were identified beneath the lamina propria when the epithelium was injured and remodeled, or in vitro when the epithelial cells were dedifferentiated.

Rapid procedure developed for direct immune electron microscopy

1991 ◽  
Vol 49 ◽  
pp. 298-299
Author(s):  
C.E. Nunamaker ◽  
T.R. Haven
Keyword(s):  
Electron Microscopy ◽  
Negative Contrast ◽  
Clinical Samples ◽  
Immune Electron Microscopy ◽  
Virus Antibody ◽  
Virus Diagnosis ◽  
Virus Particles ◽  
Transmission Electron ◽  
Original Specimen ◽  
Veterinary Laboratory

Negative contrast electron microscopy (NCEM) and direct immune electron microscopy (DIEM) are used in the detection of viruses. Although NCEM provides a simple and rapid method for detecting viruses in clinical specimens, 106 virus particles per milliliter must be present in the original specimen to be detected by transmission electron microscopy. DIEM has been found to be more sensitive than conventional NCEM by forming virus-antibody aggregates which are easily visible by TEM. While a DIEM procedure is utilized for routine diagnostic bovine coronavirus detection in clinical samples submitted to the Wyoming State Veterinary Laboratory, Laramie, WY (WSVL), time-consuming incubations and periodic salt precipitations (Fig. 2) have sometimes delayed the desired rapid turn-around time for TEM virus diagnosis. To help avoid these delays, modifications to the DIEM procedure have been made.

Isolation of Single Stranded DNA from Purified Hepatitis A-Virus

1978 ◽  
Vol 33 (7-8) ◽  
pp. 594-597 ◽  
Author(s):  
Bertram Flehmig ◽  
Hans Dieter Royer ◽  
Hans-Joachim Gerth
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Class I ◽  
Immune Electron Microscopy ◽  
Single Stranded Dna ◽  
Virus Particles ◽  
Size Classes ◽  
Length Measurements

Abstract Hepatitis A-Yirus-Single-Sranded D N A , Electron Microscopy, Length Measurements, Parvovirus Hepatitis A-virus was purified from human stools by three purification steps. Virus was identified by radioimmuno-assay and purity monitored with immune electron microscopy. Virus particles, serologically and morphologically identical, banded in CsCl in two density ranges at 1.31 — 1.34 g/cm3 and at 1.41 — 1.43 g/cm3. Virions of density 1.31 — 1.34 g/cm3 were shown to contain single-stranded D N A of different size classes. Class I 1.33 kb, class I I 4.61 kb in addition a small amount of molecules was de­ tected with lengths up to 15 kb.

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