scholarly journals Effect of Thymidine Auxotrophy, Thymidine Starvation and Nalidixic Acid Inhibition on the Properties of DNA Labelled by a Pulse of [3H]Thymidine in Staphylococcus aureus

Microbiology ◽  
1980 ◽  
Vol 120 (1) ◽  
pp. 161-171
Author(s):  
C. M. THOMAS ◽  
K. G. H. DYKE
Keyword(s):  
Staphylococcus Aureus ◽  
Nalidixic Acid ◽  
Acid Inhibition ◽  
Thymidine Auxotrophy

scholarly journals STUDY OF BACTERIA ISOLATED FROM URINARY TRA INFECTION AND THEIR SENSITIVITY PATTERN

2004 ◽  
Vol 43 (154) ◽  
Author(s):  
Aarati Karki ◽  
B R Tiwari ◽  
S B Pradhan
Keyword(s):  
Staphylococcus Aureus ◽  
Retrospective Study ◽  
Nalidixic Acid ◽  
Urine Sample ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Klebsiella Species ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Proteus Species

A retrospective study was conducted among out-patient and in-patient in Kathmandu medical collegeTeaching hospital of one month from the duration 2060-11-15 to 2060-12 -15. Mid-stream urine [MSU] of300 patient were cultured. Out of these, 75 patients’ urine were found to have significant bacterial growth.Total of five species of bacterial species isolated were viz.E.coli 37(33.3%), Proteus species 25(27.7%),Klebsiella species 15(16.6%), Staphylococcus aureus 8(8.8%) and Pseudomonas aureginosa 1(1.1%).Regarding their antibiotic sensitivity pattern, E. coli were sensitive to Nitrofurantoin 31 (83.8% ), Norfloxacin25 (67.5%), Ofloxacin 30(81.0%), Amoxycillin 16(43.2%), Nalidixic acid 15 (40.5%).proteus species weresensitive to Nitrofurantoin 15 (60.0%), Norfloxacin 15 (60.0%), Ofloxacin 12(48.0%), Amoxycillin 13(52.0%),Nalidixic acid 10 (40.0%), Ciprofloxacillin 12(48.0%). Klebsiella species were sensitive to Nitrofurantoin 9(60.0%), Norfloxacin 5 (33.3%), Oflaxin 6(40.0%), Amoxycillin 5(33.3%), Nalidixic acid 4 (26.6%),ciprofloxacillin 6(40.0%).Staphylloccus aureus were sensitive to Nitrofurantoin 8(100.0%), Norfloxacin 6(75.0%), Oflaxin 8(100.0%),Amoxycillin 7(87.5%), Nalidixic acid 6(75.0%), Ciprofloxacillin 8(100 %). And Pseudomonas aureginosawere resistant to all antibiotics which we are using in this research work.Key Words: Urine sample, Identification, Sensitivity pattern.

scholarly journals Novel Ciprofloxacin-Resistant, Nalidixic Acid-Susceptible Mutant of Staphylococcus aureus

2002 ◽  
Vol 46 (7) ◽  
pp. 2276-2278 ◽  
Author(s):  
Laura J. V. Piddock ◽  
Yu Fang Jin ◽  
Mark A. Webber ◽  
Martin J. Everett
Keyword(s):  
Staphylococcus Aureus ◽  
Nalidixic Acid ◽  
Promoter Region ◽  
Efflux Pump ◽  
Multidrug Efflux ◽  
Multidrug Efflux Pump

ABSTRACT A ciprofloxacin-resistant, nalidixic acid-susceptible mutant of Staphylococcus aureus (F145) contained no mutations within gyrA, gyrB, grlA, and grlB or within norA or its promoter region. MICs and accumulation studies suggest the role of a novel multidrug efflux pump.

scholarly journals In Vitro Activities of Six Quinolones and Mechanisms of Resistance in Staphylococcus aureus and Coagulase-Negative Staphylococci

2001 ◽  
Vol 45 (5) ◽  
pp. 1553-1557 ◽  
Author(s):  
Hans-Jörg Linde ◽  
Mario Schmidt ◽  
Emmi Fuchs ◽  
Udo Reischl ◽  
Hans-Helmut Niller ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Amino Acid ◽  
Nalidixic Acid ◽  
Quinolone Resistance ◽  
Amino Acid Substitutions ◽  
Mechanisms Of Resistance ◽  
Coagulase Negative Staphylococci ◽  
Active Efflux ◽  
Resistance Phenotype

ABSTRACT Of 94 clinical isolates of Staphylococcus aureus(n = 51) and coagulase-negative staphylococci (CNS) (n = 43), mutations in the quinolone resistance-determining region of topoisomerases GrlA, GrlB, GyrA, and GyrB together with MICs of six quinolones were analyzed. Amino acid substitutions at identical residues (GrlA residues 80 and 84; GyrA residues 84 and 88) were found in S. aureus and CNS. Active efflux, as suggested by blocking by reserpine, contributed substantially to the resistance phenotype in some strains. Among ciprofloxacin, clinafloxacin, levofloxacin, nalidixic acid, trovafloxacin, and sparfloxacin, a 0.5-μg/ml concentration of sparfloxacin discriminated best between strains with two or three mutations and those with no mutations.

Evaluation of a Selective Broth for Detection of Staphylococcus aureus Using Impedance Microbiology

2001 ◽  
Vol 64 (1) ◽  
pp. 44-50 ◽  
Author(s):  
KIRSTEN E. GLASSMOYER ◽  
SCOTT M. RUSSELL
Keyword(s):  
Staphylococcus Aureus ◽  
Nalidixic Acid ◽  
Bacterial Species ◽  
Nutrient Broth ◽  
Microbiological Method ◽  
E Coli ◽  
Poultry Products ◽  
Chicken Carcass ◽  
Low Levels ◽  
Staphylococcus Spp

Experiments were conducted to evaluate a selective nutrient broth containing acriflavine and nalidixic acid for detection of Staphylococcus aureus using an impedance microbiological method. Nine species of bacteria, other than S. aureus, were evaluated using the selective broth to determine if these species could be inhibited. A total of 10 ppm of nalidixic acid inhibited the gram-negative species tested, with the exception of Pseudomonas aeruginosa. Similarly, 10 ppm of acriflavine suppressed the Staphylococcus spp. examined; however, S. aureus retained the ability to proliferate. Nutrient broth solution containing 10 ppm of nalidixic acid and 10 ppm of acriflavine (S. aureus impedance broth [SIB]) inhibited multiplication of most of the bacterial species tested and allowed S. aureus to be detected in an average of 16.4 h. Fresh chicken carcass rinses and cooked chicken rinses were inoculated with Escherichia coli and S. aureus and assayed using SIB in conjunction with impedance. Results demonstrated that S. aureus could be detected in less than 11.5 h, although the presence of E. coli decreased detection times. Additionally, impedance assays were conducted using five different poultry products to evaluate the sensitivity of the broth for detecting S. aureus. S. aureus could be detected on poultry products when present at low levels (101 CFU/ml) in less than 24 h. These studies demonstrated that SIB may be used in conjunction with impedance for rapid detection of S. aureus. However, without further modification, this method should not be used for enumeration of S. aureus from samples containing mixed microflora.

Rapid Determination of Listeria monocytogenes in Foods Using a Resuscitation/Selection/Kit System Detection

1993 ◽  
Vol 76 (3) ◽  
pp. 632-634 ◽  
Author(s):  
Alain Martin ◽  
Stanley E Katz
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Nalidixic Acid ◽  
Yeast Extract ◽  
Rapid Determination ◽  
Sodium Pyruvate ◽  
Chicken Fat

Abstract A resuscitation medium consisting of a trypticase soy broth base supplemented with 0.5% yeast extract, 0.25% sodium pyruvate, 0.01 % sodium thiogly col late, and 0.1 % chicken fat was used in the resuscitation of heat-injured and freeze-injured cells of Listeria monocytogenes. After a resuscitation period of 4-h, the medium was made selective through the addition of nalidixic acid, acriflavin, and cycloheximide. The organisms were incubated in the selectivized medium at 35°C for an additional 16 h. The numbers of resuscitated Listeria monocytogenes cells rose from 101 to 107 cells/mL in 20 h. Similar numbers of Staphylococcus aureus, Escherichia coli, and Salmonella bonn were grown together with Listeria monocytogenes; these organisms did not inhibit the growth of Listeria monocytogenes nor interfere with its detection by the Listeria-Tek kit system. The resuscitation/selection/ kit system (RSK) was compared with the methodology in the Bacteriological Analytical Manual (BAM) for the detection of Listeria monocytogenes in 22 naturally contaminated cheese samples: 8 of these were positive by the BAM system and 12 were positive by the RSK system. The 8 Listeria positives found by the BAM system were positive by the RSK system. All 12 Listeria-presumptive positive samples by the RSK system were confirmed to be Listeria monocytogenes. The use of the RSK system enhanced the recovery of the pathogen, and detection was accomplished within 24 h.

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