Evaluation of a Selective Broth for Detection of Staphylococcus aureus Using Impedance Microbiology

2001 ◽  
Vol 64 (1) ◽  
pp. 44-50 ◽  
Author(s):  
KIRSTEN E. GLASSMOYER ◽  
SCOTT M. RUSSELL
Keyword(s):  
Staphylococcus Aureus ◽  
Nalidixic Acid ◽  
Bacterial Species ◽  
Nutrient Broth ◽  
Microbiological Method ◽  
E Coli ◽  
Poultry Products ◽  
Chicken Carcass ◽  
Low Levels ◽  
Staphylococcus Spp

Experiments were conducted to evaluate a selective nutrient broth containing acriflavine and nalidixic acid for detection of Staphylococcus aureus using an impedance microbiological method. Nine species of bacteria, other than S. aureus, were evaluated using the selective broth to determine if these species could be inhibited. A total of 10 ppm of nalidixic acid inhibited the gram-negative species tested, with the exception of Pseudomonas aeruginosa. Similarly, 10 ppm of acriflavine suppressed the Staphylococcus spp. examined; however, S. aureus retained the ability to proliferate. Nutrient broth solution containing 10 ppm of nalidixic acid and 10 ppm of acriflavine (S. aureus impedance broth [SIB]) inhibited multiplication of most of the bacterial species tested and allowed S. aureus to be detected in an average of 16.4 h. Fresh chicken carcass rinses and cooked chicken rinses were inoculated with Escherichia coli and S. aureus and assayed using SIB in conjunction with impedance. Results demonstrated that S. aureus could be detected in less than 11.5 h, although the presence of E. coli decreased detection times. Additionally, impedance assays were conducted using five different poultry products to evaluate the sensitivity of the broth for detecting S. aureus. S. aureus could be detected on poultry products when present at low levels (101 CFU/ml) in less than 24 h. These studies demonstrated that SIB may be used in conjunction with impedance for rapid detection of S. aureus. However, without further modification, this method should not be used for enumeration of S. aureus from samples containing mixed microflora.

scholarly journals STUDY OF BACTERIA ISOLATED FROM URINARY TRA INFECTION AND THEIR SENSITIVITY PATTERN

2004 ◽  
Vol 43 (154) ◽  
Author(s):  
Aarati Karki ◽  
B R Tiwari ◽  
S B Pradhan
Keyword(s):  
Staphylococcus Aureus ◽  
Retrospective Study ◽  
Nalidixic Acid ◽  
Urine Sample ◽  
Bacterial Species ◽  
Antibiotic Sensitivity ◽  
Klebsiella Species ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Proteus Species

A retrospective study was conducted among out-patient and in-patient in Kathmandu medical collegeTeaching hospital of one month from the duration 2060-11-15 to 2060-12 -15. Mid-stream urine [MSU] of300 patient were cultured. Out of these, 75 patients’ urine were found to have significant bacterial growth.Total of five species of bacterial species isolated were viz.E.coli 37(33.3%), Proteus species 25(27.7%),Klebsiella species 15(16.6%), Staphylococcus aureus 8(8.8%) and Pseudomonas aureginosa 1(1.1%).Regarding their antibiotic sensitivity pattern, E. coli were sensitive to Nitrofurantoin 31 (83.8% ), Norfloxacin25 (67.5%), Ofloxacin 30(81.0%), Amoxycillin 16(43.2%), Nalidixic acid 15 (40.5%).proteus species weresensitive to Nitrofurantoin 15 (60.0%), Norfloxacin 15 (60.0%), Ofloxacin 12(48.0%), Amoxycillin 13(52.0%),Nalidixic acid 10 (40.0%), Ciprofloxacillin 12(48.0%). Klebsiella species were sensitive to Nitrofurantoin 9(60.0%), Norfloxacin 5 (33.3%), Oflaxin 6(40.0%), Amoxycillin 5(33.3%), Nalidixic acid 4 (26.6%),ciprofloxacillin 6(40.0%).Staphylloccus aureus were sensitive to Nitrofurantoin 8(100.0%), Norfloxacin 6(75.0%), Oflaxin 8(100.0%),Amoxycillin 7(87.5%), Nalidixic acid 6(75.0%), Ciprofloxacillin 8(100 %). And Pseudomonas aureginosawere resistant to all antibiotics which we are using in this research work.Key Words: Urine sample, Identification, Sensitivity pattern.

scholarly journals Isolation and characterization of bacteria residing in the oral, gut, and fecal samples of different pheasant species

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Mushtaq ◽  
S. M. Bukhari ◽  
S. Ahmad ◽  
A. Khattak ◽  
M. B. Chattha ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Gut Contents ◽  
Phasianus Colchicus ◽  
Gut Content ◽  
E Coli ◽  
Colony Forming Unit ◽  
Isolation And Characterization ◽  
Staphylococcus Spp ◽  
Campylobacter Spp

Abstract There is a paucity of research conducted on microbial prevalence in pheasants. The microbiota of captive birds has zoonotic significance and must be characterize. Present study is therefore planned to assess the microbiota from oral, fecal and gut content of captive avian species. It will be helpful in characterization of harmful microbes. Different samples taken from oral, gut and feces of ring-necked pheasants (Phasianus colchicus), green pheasants (Phasianus versicolor), golden pheasant (Chrysolophus pictus) and silver pheasant (Lophura nycthemera). Samples were collected, diluted, and inoculated onto different agar plates (MacConkey, SS agar, MSA and nutrient agar) for cultivation of bacterial species. Colonies of E.coli, Staphylococcus spp. Brachyspira spp. and Campylobacter spp were observed based on colony morphology. Colony forming unit showed E. coli as frequently found bacteria in fecal, oral and gut contents of all the above pheasants. The overall significance difference was found among bacterial species of golden pheasants, green pheasant, ring-necked pheasant, and silver pheasants. It was concluded that E.coli is predominant isolated from heathy pheasants followed by Campylobacter, Staphylococcus and Brachyspira.

scholarly journals Bacterial Contamination Associated with Retail Chicken Carcasses in Osogbo, Nigeria

2015 ◽  
Vol 05 (04) ◽  
pp. 045-050
Author(s):  
Adesiji Yemisi Olukemi ◽  
Igbinigie Mavis Osas ◽  
Olaitan Janet Olubukola ◽  
Ogah Ikhevha Jeremiah
Keyword(s):  
Diffusion Method ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Poultry Products ◽  
Bacterial Contaminants ◽  
Food Borne ◽  
Chicken Carcass ◽  
Hygiene Measures ◽  
Food Borne Illness

Abstract Background: Worldwide, food borne illness is often associated with consumption of meat and poultry products sold at retail markets. A study on the bacteriological status of chicken carcass in Osogbo, Nigeria, was carried out to determine the prevalence of Arcobacter species, Escherichia coli and Staphylococcus aureus in chicken carcasses. Methodology: A total of 100 samples of chicken carcasses were collected from two major processing points in Osogbo, Nigeria. The samples were analysed for the presence of bacterial contaminants using standard microbiological isolation and identification procedures, with antimicrobial susceptibility test performed using the disk diffusion method. Result: Of hundred chicken carcasses sampled, 38% were positive for Arcobacter species and E. coli while 60% accounted for S. aureus isolates. Ninety percent of Arcobacter spp isolates were susceptible to ciprofloxacin, 85% to gentamicin, and pefloxacin, 70% to chloramphenicol and 90% were resistant to amoxicillin, 85% to augumentin and 80% to streptomycin. Hundred percent of E. coli isolates were susceptible to ciprofloxacin, pefloxacin, 95% to gentamicin and 100% were resistant to streptomycin, 85 % resistant to amoxicillin, augumentin, while 100% of S. aureus isolates were susceptible to trimethoprim sulphamethoxazole, 90% susceptible to gentamicin, 80% to streptomycin and 100% of the S.aureus isolates were resistant to ampliclox. Conclusion: The bacteriological status of chicken carcass revealed high contamination with Arcobacter, E coli and S. aureus with varying degree of antibiotic resistance therefore, improvement in meat processing procedures and strict hygiene measures towards reduction of these pathogens in food products should be encouraged.

scholarly journals Value of polymerase chain reaction in etiological diagnostic of infectiove endocarditis

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
E O Kotova ◽  
E A Domonova ◽  
A S Pisaryuk ◽  
O Y Silveystrova ◽  
Y L Karaulova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Microbiological Method ◽  
Chain Reaction ◽  
E Coli ◽  
Enterococcus Spp ◽  
Acinetobacter Spp ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Staphylococcus Spp ◽  
Culture Negative

Abstract Introduction Identification of a causative agent in patients with infective endocarditis (IE) is crucial for diagnostic and prescribing etiotropic therapy which defines positive outcome of a disease. High rate of a culture negative IE and inaccurate results of traditional microbiological methods raise a concern. So the methods of etiological diagnostics in IE are in need of development, particularly introduction of polymerase chain reaction (PCR) method might be helpful. Aim Modernisation of the algorithm of IE etiological diagnostic by introducing PCR Materials and methods The study included 85 cases of IE [first episode of IE (n=79), recurrence/relapse (n=6)] verified by DUKE criteria 2009, 2015, hospitalized in Moscow primary hospital from 2012 to 2017. All patient had venous blood investigated both with microbiological method and with broadrange and specific PCR. Following microorganisms' DNA were assessed by PCR: Staphylococcus spp. (MRCoNS, S. aureus and others), Streptococcus spp. (S. agalactiae, S. pyogenes and others), Enterococcus spp. (E. faecium, E. faecalis and others), Enterobacteriaceae, Klebsiella spp. (K. pneumoniae and others), E. coli, Proteus spp., A. baumanii, P. aeruginosa, Fungi (C. albicans, C. glabrata, Aspergillus spp. and others). Results Median age was 55.48 years (95% confidence interval (CI) 51.16–59.8), males 67.9%. History of cardiovascular diseases was in 54 (68.35%), diabetes mellitus in 18 (22.78%), hepatitis B and/or C in 31 (39.2%), intravenous drug dependency in 27 (34.18%), chronic kidney disease in 38 (48.1%), median Charlson comorbidity index was 5.44 (95% CI 4.52–6.37). Left-side IE was in 50 (63.29%), right-side IE – in 23 (29.12%), left-right-side IE in 6 (7.59%). Secondary IE was in 53 patients (62.3%). Embolic events were diagnosed in 27 cases (34.18%), in-hospital mortality – in 22 (27.8%). Microbiological method identified etiological agent in 55 of 85 cases (61.2%), featuring Staphylococcus aureus (n=23), Staphylococcus CoNS (n=6), Escherichia coli (n=1), Acinetobacter spp. (n=2), Streptococcus spp. (n=2), Enterococcus spp. (n=8), Klebsiella pneumoniae (n=2), Gemella haemolysans (n=2), several causative agents (n=6). Additional PCR testing identified etiology in 14 of 33 (42.2%) featuring Staphylococcus spp. (n=6), Enterococcus spp. (n=3), Streptococcus spp. (n=1), Aspergillus sp. (n=1) Pasteurella multocida (n=1), Enterococcus spp. + Staphylococcus spp. (n=1), Staphylococcus spp.+ A. baumanii + E. coli (n=1). PCR method identified 6 fals-positive results of microbilogical investigation [S. epidermidis (n=2), G. haemolysans, Acinetobacter spp., E. faecalis, K. pneumoniae], that are most probably due to preanalytical sample contamination Conclusions Introduction of PCR into the algorithm of IE etiological diagnostic increased validity of laboratory findings on 23.5%. True culture negative IE was present in 19 of 85 patients. Rate of mortality and complications in IE remains high. FUNDunding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): Peoples' Friendship University of Russia (RUDN), Moscow, Russia

scholarly journals Comparative bacteriological analysis of frozen and fresh chicken meats sold around old site Bayero University Kano

2021 ◽  
Vol 14 (1) ◽  
pp. 258-262
Author(s):  
Habibu Usman Abdu ◽  
Aisha Mustapha Abubakar
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chicken Meat ◽  
Salmonella Spp ◽  
E Coli ◽  
Meat Samples ◽  
And Staphylococcus Aureus ◽  
Klebsiella Spp ◽  
Campylobacter Spp

Foodborne pathogens are serious public healthproblems that greatly affect the cost of food production and healthcare while poultry are often associated with foodborne disease outbreaks. This study aimed at investigating the occurrence and distribution of bacterial pathogens associated with chicken meats sold around Bayero University, Kano old site. A total of twenty (20) meat samples comprised of 10 frozen and ten freshly slaughtered chicken were purchased from different selling outlets around the study area. The samples were processed using standard cultural and biochemical procedures. The results of the study showed the mean aerobic mesophilic bacterial count of 5.93 x 106 CFU/g and 3.12 x 106  CFU/g for frozen and freshly slaughtered chicken meat samples respectively. The coliform counts were 420.1 and 347.7 CFU/100g for frozen and freshly slaughtered chicken meat samples respectively. The distribution of different bacterial species revealed E. coli (26.0%), Klebsiella spp (4.0%), Salmonella spp (19.5%), Campylobacter spp. (14.6%) and Staphylococcus aureus (30%) in frozen chicken meat samples while freshly slaughtered chicken meat samples revealed E. coli (21.0%), Klebsiella spp (8.9%), Salmonella spp (30%), Campylobacter spp. (15.0%) and Staphylococcus aureus (24.0%). This showed that frozen chicken meats were more contaminated than freshly slaughtered chicken meats which might be associated with poor handling and storage conditions.

scholarly journals Detection of bacterial species from clinical mastitis in dairy cows at Nilphamari district and their antibiogram studies

2017 ◽  
Vol 2 (4) ◽  
pp. 656-663 ◽  
Author(s):  
Md Titon Mia ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Shahin Mahmud ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Bacterial Species ◽  
Clinical Mastitis ◽  
Biochemical Characteristics ◽  
E Coli ◽  
Milk Samples ◽  
Bacillus Spp ◽  
Staphylococcus Spp

The present study was conducted on the rural dairy cows to detect the bacterial species from clinical mastitis in dairy cows with their antibiogram studies during the period from January 2015 to June 2015. For this purpose two upazilla were selected under the Nilphamari district. On the basis of morphology, staining, cultural and biochemical characteristics, the isolated organisms were classified as, Staphylococcus spp., Streptococcus spp., E. coli, and Bacillus spp. .For this study, a total of 48 samples were collected from affected mastitis cows. Out of 48 milk samples, 17 were positive for Staphylococcus spp. (35.42%), 9 were positive for Streptococcus spp. (18.75%), 7 were positive for E. coli (14.58%), 5 were positive for Bacillus spp.(10.41%),5 were positive for mixed organisms (10.41%) and 5 were unidentified organisms (10.41%). Antibiogram studies revealed that all of the isolates of Staphylococcus spp. were sensitive to gentamicin and were resistant to streptomycin. Gentamicin was sensitive to all of the isolates of Streptococcus spp. and was resistant to streptomycin. The isolates of Bacillus spp. were sensitive to ciprofloxacin and Bacillus spp. was resistant to streptomycin. All of the isolates of E. coli were sensitive to ciprofloxacin the isolates were resistant to ampicillin and amoxicillin. Over all sensitivity revealed that ciprofloxacin, gentamicin and enrofloxacin were most efficacious. Thus, it may be recommended that ciprofloxacin, gentamicin and enrofloxacin in optimum doses would resolve most cases of clinical mastitis in dairy cows.Asian J. Med. Biol. Res. December 2016, 2(4): 656-663

Survival of Staphylococcus aureus and Escherichia coli as Affected by Ethanol and NaCl

2001 ◽  
Vol 64 (4) ◽  
pp. 546-550 ◽  
Author(s):  
SUE-LANG HUANG ◽  
YIH-MING WENG ◽  
ROBIN Y.-Y. CHIOU
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Limited Range ◽  
Nutrient Broth ◽  
Nutrient Agar ◽  
Ethanol Sensitivity ◽  
E Coli ◽  
Viable Cells

Growth of three strains of Staphylococcus aureus and two strains of Escherichia coli on nutrient agar (NA) supplemented with ethanol and NaCl was investigated. S. aureus did not grow on NA containing ≧10% ethanol (wt/wt) combined with ≧0% NaCl (wt/wt), or 7.5% ethanol combined with 7.5% NaCl. Neither E. coli nor E. coli O157:H7 grew on NA containing ≧7.5% ethanol combined with ≧0% NaCl, 5% ethanol combined with ≧2.5% NaCl, or ≧5% NaCl combined with ≧0% ethanol. It is apparent that NaCl enhanced the inhibitory effect of ethanol on growth of S. aureus and E. coli. When cells were suspended in nutrient broth containing 12.5, 20, or 40% ethanol combined with NaCl, viable cells decreased with an increase of ethanol concentration. Ethanol sensitivity among strains and between genera varied in a limited range. When the cells were exposed to 20% ethanol in combination with 5% NaCl, S. aureus and E. coli lost viability after 30 and 10 min, respectively. When treated with 40% ethanol combined with ≧0% NaCl, all test strains lost viability within 5 min.

scholarly journals Study the antibiotics sensitivity and beta- lactamase productivity of some Staphylococcus spp. Isolates from different sources of the Al Jamhoree Teaching Hospital in Mosul City

2019 ◽  
Vol 24 (7) ◽  
pp. 27
Author(s):  
Ayman Mohammed Khaleel1 ◽  
Adeeba Younis Shareef ◽  
, Shakeeba Younis Shareef
Keyword(s):  
Staphylococcus Aureus ◽  
Teaching Hospital ◽  
Clinical Laboratory ◽  
Bacterial Species ◽  
Age Groups ◽  
National Committee ◽  
Beta Lactamase ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Staphylococcus Spp

The study includes the isolation of certain types of Gram-positive bacteria Staphylococcus spp..The sample materials (blood, wounds, burns) were collected from both genders of all age groups of inpatients in Al Jamhoree Teaching Hospital in the Mosul during June till end of November 2018. Staphylococcus aureus was the most common among the isolates with 7 isolates(38.9%) followed by Staphylococcus epidermidis, Staphylococcus scriuri Staphylococcus lentus (Staphylococcus simulans) with 2 isolates each with 11.1% followed by Staphylococcus chromogenase, Staphylococcu scapilis and  Staphylococcus xylosus with one isolate for each one 5.5%. The number of isolates was 9 isolates and 50%, followed by wound samples. The isolates were 6 isolated by 33% and the samples of burns were 3 isolates. 17% were isolated and Staphylococcus aureus was the most dominant species. The sensitivity of bacterial isolates was  studied for 15 antibiotics for different antibiotic groups. The results showed that a difference in the resistance ratio of the isolates to these antagonists, as they were resistant to the Ceftrixone, Cloxacillin 100%. The Ciprofloxacillin, Gentamycin, Pipracillin, and Amikacin resistance were  reduced. The Ciprofloxacillin antagonist was the most affected on the bacterial isolates studied, followed by the Rifampin. The results showed that the beta-lactamase enzyme was not produced by any of the Iodic methods by 4 bacterial strains, while the rest of the species varied in the susceptibility of production to the enzyme. The Iodine tube is one of the best methods to detect the production of these enzymes. The susceptibility of bacterial species to the production of large-spectrum beta-lactase enzymes was also tested using the National Committee for Clinical Laboratory Standards (NCCL) and the double-disc method. Staphylococcus lentus, Staphylococcus capilis, Staphylococcus chromanogenes The first NCCL has shown a single isolation of Staphylococcus capilis ability to produce it.   http://dx.doi.org/10.25130/tjps.24.2019.125

scholarly journals A bacteriological study of septicemia in Hilla city

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Rana S. Al-Taweel
Keyword(s):  
Staphylococcus Aureus ◽  
Immunosuppressive Treatment ◽  
Coagulase Negative Staphylococcus ◽  
Bacteriological Study ◽  
E Coli ◽  
Causative Agents ◽  
Life Threatening ◽  
Antibiotics Sensitivity ◽  
Intravascular Devices ◽  
Staphylococcus Spp

Septicemia is a serious bloodstream infection; it can quickly become life-threatening.  The current study aimed to isolate and identify the causative agents of septicemia cases with reference to the antibiogram test.  52 blood samples were collected from a number of incomings and those who lie in Hilla general hospital. After incubation and culturing on suitable media, 30 samples gave growth of one or two species of bacteria. A total of 33 bacterial isolates were obtained, most of them (16 isolates) were belong to coagulase-negative Staphylococcus spp., followed by E. coli (6 isolates), Pseudomonas sp. (5 isolates), Streptococcus sp. (2 isolates), and one isolate for each of Staphylococcus aureus,  Streptococcus pneumonia, Acinetobacter sp., and Listeria monocytogenes. When antibiotics sensitivity test was accomplished, most Staphylococcus spp. were sensitive for daptomycin and had high resistance to both of vancomycin and clindamycin, whereas Staphylococcus aureus was sensitive for most the used antibiotics. Half of E. coli isolates were sensitive, while the second half were resistant to the used antibiotics. Imipenem inhibited the growth of all Pseudomonas isolates, whereas 80% of them were resistant to amikacin. The epidemiology of bacteremia is altering with the aging of the population, shifts in healthcare, and progress in medicine, such as increased use of immunosuppressive treatment, intravascular devices, and invasive procedures.

scholarly journals Prevalence and Characterization of Plasmid-mediated Quinolone Resistance Genes among Escherichia coli Strains Isolated from Different Water Sources in Alborz Province, Iran

2019 ◽  
Vol 11 (1) ◽  
pp. 36-41
Author(s):  
Reza Ranjbar ◽  
Shahrzad Tavanania ◽  
Azar Sabokbar ◽  
Faham Khamesipour
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Nalidixic Acid ◽  
Bacterial Species ◽  
Water Sources ◽  
Quinolone Resistance ◽  
E Coli ◽  
Different Water Sources

BACKGROUND: This study was conducted to investigate the prevalence of quinolone resistance associated (qnr) antibiotic resistance among Escherichia coli strains isolated from different water sources in Alborz province, Iran.METHODS: E. coli strains were isolated and identified by standard microbiological and biochemical tests from surface water sources in Alborz province, Iran in 2013. Fluoroquinolone-resistant isolates were determined using the antimicrobial susceptibility test determined by the Kirby–Bauer assay. Total genomic and plasmid DNA were extracted by boiling method. The presence of qnr genes in all nalidixic-acid and ciprofloxacin-resistant E. coli strains was determined by Polymerase Chain Reaction (PCR). The PCR amplicons were visualized after electrophoresis stained with ethidium bromide.RESULTS: One hundred E. coli strains were isolated from the water sources examined in this study. As much as 22.7% and 7.3% of the isolates were resistant to nalidixic acid and ciprofloxacin respectively. While qnrS, qnrB and qnrA genes were detected in 28%, 9% and 1% of fluoroquinolone-resistant isolates respectively. All fluoroquinolone-susceptible isolates however did not contain any of the qnr genes.CONCLUSION: This study reflects an increasing prevalence of fluoroquinolone-resistant E. coli strains in surface water sources. Underlining the importance of surface water sources as reservoirs for dissemination of potentially pathogenic E. coli and horizontal gene transfer between other waterborne bacterial species. Other possible mechanisms of resistance should also be investigated for better characterization of quinolone-resistant E. coli isolates. Therefore, immediate measures are needed to control and treat water sources more effectively.KEYWORDS: antibiotic resistance, E. coli, qnr genes, water sources 

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