scholarly journals Induction of Bacteriophage from Members of the Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium scrofulaceum Serocomplex

Microbiology ◽  
1984 ◽  
Vol 130 (8) ◽  
pp. 2059-2066 ◽  
Author(s):  
T. L. TIMME ◽  
P. J. BRENNAN
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Intracellulare

scholarly journals Factors Influencing the Chlorine Susceptibility of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum

2003 ◽  
Vol 69 (9) ◽  
pp. 5685-5689 ◽  
Author(s):  
Joseph O. Falkinham
Keyword(s):  
Growth Rate ◽  
Mycobacterium Avium ◽  
Growth Phase ◽  
Growth Rates ◽  
Growth Stage ◽  
Tween 80 ◽  
Culture Conditions ◽  
C Cells ◽  
Mycobacterium Intracellulare ◽  
Factors Influencing

ABSTRACT The susceptibility of representative strains of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (the MAIS group) to chlorine was studied to identify factors related to culture conditions and growth phase that influenced susceptibility. M. avium and M. intracellulare strains were more resistant to chlorine than were strains of M. scrofulaceum. Transparent and unpigmented colony variants were more resistant to chlorine than were their isogenic opaque and pigmented variants (respectively). Depending on growth stage and growth rate, MAIS strains differed in their chlorine susceptibilities. Cells from strains of all three species growing in early log phase at the highest growth rates were more susceptible than cells in log and stationary phase. Rapidly growing cells were more susceptible to chlorine than slowly growing cells. The chlorine susceptibility of M. avium cells grown at 30°C was increased when cells were exposed to chlorine at 40°C compared to susceptibility after exposure at 30°C. Cells of M. avium grown in 6% oxygen were significantly more chlorine susceptible than cells grown in air. Chlorine-resistant MAIS strains were more hydrophobic and resistant to Tween 80, para-nitrobenzoate, hydroxylamine, and nitrite than were the chlorine-sensitive strains.

Mycobacterium Avium And Mycobacterium Intracellulare: Distinct Pathogens Or Just A “complex”?

2010 ◽  
Author(s):  
Matthew J. Heffer ◽  
Fahad Al-Ghimlas ◽  
Victor Hoffstein ◽  
Frances Jamieson ◽  
Mauli Mehta ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Intracellulare

Difference in drug susceptibility distribution and clinical characteristics between Mycobacterium avium and Mycobacterium intracellulare lung diseases in Shanghai, China

2021 ◽  
Vol 70 (5) ◽  
Author(s):  
Weiping Wang ◽  
Jinghui Yang ◽  
Xiaocui Wu ◽  
Baoshan Wan ◽  
Hongxiu Wang ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Antimicrobial Susceptibility ◽  
Type Species ◽  
Clinical Characteristics ◽  
Radiographic Findings ◽  
Content Type ◽  
Mycobacterium Intracellulare ◽  
Link Type ◽  
Broth Microdilution Method ◽  
Significant Difference

Introduction. Mycobacterium avium complex (MAC) has been reported as the most common aetiology of lung disease involving nontuberculous mycobacteria. Hypothesis. Antimicrobial susceptibility and clinical characteristics may differ between Mycobacterium avium and Mycobacterium intracellulare . Aim. We aimed to evaluate the differences in antimicrobial susceptibility profiles between two major MAC species ( Mycobacterium avium and Mycobacterium intracellulare ) from patients with pulmonary infections and to provide epidemiologic data with minimum inhibitory concentration (MIC) distributions. Methodology. Between January 2019 and May 2020, 45 M. avium and 242 M . intracellulare isolates were obtained from Shanghai Pulmonary Hospital. The demographic and clinical characteristics of patients were obtained from their medical records. The MICs of 13 antimicrobials were determined for the MAC isolates using commercial Sensititre SLOWMYCO MIC plates and the broth microdilution method, as recommended by the Clinical and Laboratory Standards Institute (CLSI; Standards M24-A2). MIC50 and MIC90 values were derived from the MIC distributions. Results. M. intracellulare had higher resistance rates than M. avium for most tested antimicrobials except clarithromycin, ethambutol, and ciprofloxacin. Clarithromycin was the most effective antimicrobial against both the M. avium (88.89 %) and M. intracellulare (91.32 %) isolates, with no significant difference between the species (P=0.601). The MIC90 of clarithromycin was higher for M. avium (32 µg ml−1) than M. intracellulare (8 µg ml−1). The MIC50 of rifabutin was more than four times higher for M. intracellulare (1 µg ml−1) than M. avium (≤0.25 µg ml−1). The percentages of patients aged >60 years and patients with sputum, cough, and cavitary lesions were significantly higher than among patients with M. intracellulare infection than M. avium infections. Conclusions. The pulmonary disease caused by distinct MAC species had different antimicrobial susceptibility, symptoms, and radiographic findings.

scholarly journals Feature of Adhesins Produced by Human Clinical Isolates of Mycobacterium intracellulare, Mycobacterium intracellulare subsp. chimaera and Closely Related Species

2020 ◽  
Vol 8 (8) ◽  
pp. 1154
Author(s):  
Louise H. Lefrancois ◽  
Thierry Cochard ◽  
Maxime Branger ◽  
Olivia Peuchant ◽  
Cyril Conde ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Related Species ◽  
Fluorescent Protein ◽  
Severe Disease ◽  
A549 Cells ◽  
Clinical Isolates ◽  
Heparin Binding ◽  
Closely Related Species ◽  
Mycobacterium Intracellulare ◽  
Green Fluorescent

The Mycobacterium avium complex includes two closely related species, Mycobacterium avium and Mycobacterium intracellulare. They are opportunistic pathogens in humans and responsible for severe disease in a wide variety of animals. Yet, little is known about factors involved in their pathogenicity. Here, we identified, purified and characterized adhesins belonging to the heparin-binding hemagglutinin (HBHA) and laminin-binding protein (LBP) family from M. intracellulare ATCC13950 and examined clinical isolates from patients with different pathologies associated with M. intracellulare infection for the presence and conservation of HBHA and LBP. Using a recombinant derivative strain of M. intracellulare ATCC13950 producing green fluorescent protein and luciferase, we found that the addition of heparin inhibited mycobacterial adherence to A549 cells, whereas the addition of laminin enhanced adherence. Both HBHA and LBP were purified by heparin-Sepharose chromatography and their methylation profiles were determined by mass spectrometry. Patients with M. intracellulare infection mounted strong antibody responses to both proteins. By using PCR and immunoblot analyses, we found that both proteins were highly conserved among all 17 examined clinical M. intracellulare isolates from patients with diverse disease manifestations, suggesting a conserved role of these adhesins in M. intracellulare virulence in humans and their potential use as a diagnostic tool.

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