scholarly journals The Use of Mitotic Crossing-over for Genetic Analysis in Dictyostelium discoideum: Mapping of Linkage Group II

1975 ◽  
Vol 90 (2) ◽  
pp. 247-259 ◽  
Author(s):  
D. MOSSES ◽  
K. L. WILLIAMS ◽  
P. C. NEWELL
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Crossing Over ◽  
Group Ii

scholarly journals CHROMOSOME FRAGMENTS IN DICTYOSTELIUM DISCOIDEUM OBTAINED FROM PARASEXUAL CROSSES BETWEEN STRAINS OF DIFFERENT GENETIC BACKGROUND

Genetics ◽  
1980 ◽  
Vol 95 (2) ◽  
pp. 289-304
Author(s):  
Keith L Williams ◽  
Gillian E Robson ◽  
Dennis L Welker
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Genetic Background ◽  
Deleterious Effect ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Chromosome Fragments ◽  
Group Ii ◽  
Different Genetic Background

ABSTRACT The first aneuploid strains of Dictyostelium discoideum have been unambiguously characterized, using cytological and genetic analysis. Three independently isolated, but genetically similar, fragment chromosomes have been observed in segregants from diploids formed between haploid strains derived from the NC4 and VI2 isolates of D. discoideum. Once generated, the fragment chromosomes, all of which have Vl2-derived centromeres, can be maintained in a NC4 genetic background. Genetic evidence is consistent with the view that all three fragment chromosomes studied encompass the region from the centromere to the whiA locus of linkage group II and terminate in the interval between whiA and acrA. From cytological studies, one of the fragment chromosomes consists of approximately half of linkage group II.——We observed no deleterious effect on viability or asexual fruiting-body formation in either haploid or diploid strains carrying an additional incomplete chromosome and hence are disomic or trisomic, respectively, for part of linkage group II. The incomplete chromosome is lost at a frequency of 2 to 3% from disomic and trisomic strains, but surprisingly this loss is not increased in the presence of the haploidizing agent, benlate. A new locus (clyA), whose phenotype is altered colony morphology, is assigned to the region of linkage group II encompassed by the fragment chromosome.

motA1552, a mutation of Dictyostelium discoideum having pleiotropic effects on motility and discoidin I regulation.

Genetics ◽  
1988 ◽  
Vol 118 (3) ◽  
pp. 425-436
Author(s):  
S C Kayman ◽  
R Birchman ◽  
M Clarke
Keyword(s):  
Linkage Group ◽  
Dictyostelium Discoideum ◽  
Growth Temperature ◽  
Segregation Analysis ◽  
Pleiotropic Effects ◽  
Temperature Sensitive ◽  
Temperature Induction ◽  
Group Ii ◽  
Unstable Mutations ◽  
Axenic Growth

Abstract The Dictyostelium discoideum mutant MC2 exhibits temperature-sensitive growth, temperature-sensitive motility, and temperature induction of discoidin I synthesis. These three phenotypes of MC2 were not separated in the genetic experiments reported here. They were therefore assigned to the mutation motA1552, which was mapped to linkage group II by segregation analysis and by analysis of mitotic recombinant diploids. In one motA1552 strain, loss of motility preceded accumulation of discoidin I by 3 hr, indicating that discoidin I is not involved in generation of the motility defect. Expression of motA1552 phenotypes varied both among strains carrying the mutation, and among different clones of a particular strain. MC2 and its derivatives displayed elevated levels of recombination between whiA and acrA on linkage group II, and yielded highly unstable mutations at the acrA locus. Accumulation of large amounts of discoidin I during axenic growth of strain AX3 was found to depend on the presence of a second linkage group II mutation, daxA1551. This mutation was already present in the strain mutagenized to isolate motA1552, complicating explication of motA1552 action.

PARASEXUAL GENETIC ANALYSIS OF CELL PROPORTIONING MUTANTS OF DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1981 ◽  
Vol 99 (2) ◽  
pp. 183-196
Author(s):  
James H Morrissey ◽  
William F Loomis
Keyword(s):  
Cell Differentiation ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Double Mutant ◽  
Complementation Group ◽  
Stalk Cell ◽  
Recessive Mutation ◽  
Group Vi ◽  
Recessive Mutations

ABSTRACT Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.

scholarly journals THE USE OF RESTRICTION FRAGMENT LENGTH POLYMORPHISMS AND DNA DUPLICATIONS TO STUDY THE ORGANIZATION OF THE ACTIN MULTIGENE FAMILY IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1986 ◽  
Vol 112 (1) ◽  
pp. 27-42
Author(s):  
Dennis L Welker ◽  
K Peter Hirth ◽  
Patricia Romans ◽  
Angelika Noegel ◽  
Richard A Firtel ◽  
...  
Keyword(s):  
Linkage Group ◽  
Dictyostelium Discoideum ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Gene Copy ◽  
Actin Genes ◽  
Group I ◽  
Group Ii ◽  
Cloned Gene ◽  
Restriction Fragment Length

ABSTRACT The techniques of restriction fragment length polymorphism analysis and examination of gene copy number in duplication-bearing Dictyostelium discoideum strains have been used to map four actin genes of the wild-type strain NC4 to specific linkage groups. In part, this was accomplished by identification of restriction fragments corresponding to particular cloned actin genes using genespecific probes from unique sequence 5' and 3' untranslated regions. Cloned gene Actin 8 (designation act-8) maps to linkage group I; Actins 12 (act-12) and M6 (actM6) to linkage group II. An uncloned gene (act-100) also maps to linkage group II in the same region as actM6, as defined by a chromosomal duplication. From analysis of other wild isolates of D. discoideum, it was determined that in these isolates at least two actin genes map to linkage group I and at least four map to linkage group II. These results demonstrate the utility of molecular techniques in genetic analysis of Dictyostelium, particularly for developmentally regulated genes that have been cloned but that have no identified mutant phenotypes.

scholarly journals GENETIC ANALYSIS OF THE GENE FOR N-ACETYLGLUCOSAMINIDASE IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1978 ◽  
Vol 88 (2) ◽  
pp. 277-284 ◽  
Author(s):  
William F Loomis
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Structural Gene ◽  
Regulatory Protein ◽  
Group Iv ◽  
Single Locus ◽  
Stages Of Development ◽  
Separate Locus

ABSTRACT Three independent mutations affecting N-acetylglucosaminidase in Dictyostelium discoideum were mapped by the parasexual system and found to lie on linkage group IV. These mutations as well as two others were found to be recessive and noncomplementing in heterozygous diploids. Thus they all appear to affect the nagA locus. Since two of the mutations give rise to thermolabile enzyme, this defines the structural gene for N-acetylglucosaminidase. The enzyme is a homodimer of a 68,000 dalton subunit and thus would be expected to be determined by a single locus. The expression of this gene is regulated by the stages of development; however, it should be mentioned that none of the mutations fell in a separate locus that might determine a specific positive regulatory protein.

scholarly journals Genetic analysis by means of the parasexual cycle in Aspergillus niger

1967 ◽  
Vol 10 (1) ◽  
pp. 45-61 ◽  
Author(s):  
Pol Lhoas
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Sexual Cycle ◽  
Crossing Over ◽  
Linkage Groups ◽  
Parasexual Cycle ◽  
Sexual Species ◽  
Data Support ◽  
Almost All

An investigation of mitotic segregation and recombination in A. niger gave the following results:1. Thirty-one non-allelic markers have been assigned to six linkage groups (containing 11, 9, 6, 3, 1 and 1 markers respectively) by the analysis of haploid mitotic segregants from synthesized diploids.2. The sequence of nine markers in one linkage group was determined and some of the map intervals were estimated by the analysis of haploids, recombinants for linked markers.3. Almost all the haploid segregants were obtained on medium supplemented with the aminoacid analogue, p-fluoro-phenylalanine, the action of which is interpreted as an induction of chromosome losses.4. The rates of mitotic crossing-over and haploidization are much higher than in the sexual species A. nidulans and the data support Pontecorvo's (1958) suggestion that the parasexual cycle can be a substantial alternative to the sexual cycle.

scholarly journals The discoidin I gene family of Dictyostelium discoideum is linked to genes regulating its expression.

Genetics ◽  
1988 ◽  
Vol 119 (3) ◽  
pp. 571-578
Author(s):  
D L Welker
Keyword(s):  
Linkage Group ◽  
Gene Family ◽  
Dictyostelium Discoideum ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Regulatory Element ◽  
Cellular Slime Mold ◽  
Regulatory Loci ◽  
Group Ii ◽  
Restriction Fragment Length

Abstract The discoidin I protein has been studied extensively as a marker of early development in the cellular slime mold Dictyostelium discoideum. However, like most other developmentally regulated proteins in this system, no reliable information was available on the linkage of the discoidin genes to other known genes. Analysis of the linkage of the discoidin I genes by use of restriction fragment length polymorphisms revealed that all three discoidin I genes as well as a pseudogene are located on linkage group II. This evidence is consistent with the discoidin I genes forming a gene cluster that may be under the control of a single regulatory element. The discoidin I genes are linked to three genetic loci (disA, motA, daxA) that affect the expression of the discoidin I protein. Linkage of the gene family members to regulatory loci may be important in the coordinate maintenance of the gene family and regulatory loci. A duplication affecting the entire discoidin gene family is also linked to group II; this appears to be a small tandem duplication. This duplication was mapped using a DNA polymorphism generated by insertion of the Tdd-3 mobile genetic element into a Tdd-2 element flanking the gamma gene. A probe for Tdd-2 identified a restriction fragment length polymorphism in strain AX3K that was consistent with generation by a previously proposed Tdd-3 insertion event. A putative duplication or rearrangement of a second Tdd-2 element on linkage group IV of strain AX3K was also identified. This is the first linkage information available for mobile genetic elements in D. discoideum.

scholarly journals USE OF NYSTATIN-RESISTANT MUTATIONS IN PARASEXUAL GENETIC ANALYSIS IN DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1983 ◽  
Vol 104 (2) ◽  
pp. 271-277
Author(s):  
Durgadas P Kasbekar ◽  
Sanford Madigan ◽  
Eugene R Katz
Keyword(s):  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Resistance Genes ◽  
Nutrient Agar ◽  
Group V ◽  
Complementation Groups ◽  
Extreme Sensitivity ◽  
Selection Of ◽  
Nystatin Resistance

ABSTRACT Nystatin-resistant mutations exhibit extreme sensitivity to 1.3 mm coumarin. The mutations fall into three complementation groups so it is possible to select for nonallelic mutations conferring sensitivity to coumarin by selection on nystatin-containing nutrient agar plates. Complementation between such coumarin-sensitive mutations allows the selection of diploids on coumarin-containing nutrient agar. Two of the nystatin resistance genes, nysB and nysC, have been mapped tentatively to the previously unmarked linkage group V.

Sign in / Sign up

Export Citation Format

Share Document