scholarly journals Chitinibacter alvei sp. nov., isolated from stream water

2010 ◽  
Vol 60 (8) ◽  
pp. 1760-1764 ◽  
Author(s):  
Cho-Song Yang ◽  
Shih-Yi Sheu ◽  
Chiu-Chung Young ◽  
A. B. Arun ◽  
Chih-Yu Cheng ◽  
...  
Keyword(s):  
Type Strain ◽  
Stream Water ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Southern Taiwan ◽  
Physiological And Biochemical

A Gram-negative, aerobic bacterium, designated strain TNR-14T, was isolated from water of a stream located in southern Taiwan. Cells were rod-shaped and motile by means of a polar flagellum. The isolate exhibited optimum growth at 20 °C, at pH 7.0 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Chitinibacter and was related most closely to the type strain of Chitinibacter tainanensis (96.0 % similarity). The predominant cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 54.03 %) and C16 : 0 (27.22 %). The DNA G+C content of strain TNR-14T was 57.5 mol%. The results of physiological and biochemical tests and chemotaxonomic data allowed the clear phenotypic differentiation of the new isolate from Chitinibacter tainanensis. Strain TNR-14T is therefore considered to represent a novel species of the genus Chitinibacter, for which the name Chitinibacter alvei sp. nov. is proposed. The type strain is TNR-14T (=LMG 25206T =DSM 22217T =BCRC 17968T).

scholarly journals Brevundimonas halotolerans sp. nov., Brevundimonas poindexterae sp. nov. and Brevundimonas staleyi sp. nov., prosthecate bacteria from aquatic habitats

2010 ◽  
Vol 60 (8) ◽  
pp. 1837-1843 ◽  
Author(s):  
Wolf-Rainer Abraham ◽  
Andréia B. Estrela ◽  
Dennis I. Nikitin ◽  
John Smit ◽  
Marc Vancanneyt
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Aquatic Habitats ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gram Negative ◽  
Prosthecate Bacteria ◽  
Physiological And Biochemical

Eight strains of Gram-negative, bacteroid-shaped, prosthecate bacteria, isolated from brackish water (MCS24T, MCS17 and MCS35), the marine environment (CM260, CM272 and CM282) and activated sludge (FWC40T and FWC43T), were characterized using a polyphasic approach. Analysis of 16S rRNA gene sequences determined that all strains were affiliated to the alphaproteobacterial genus Brevundimonas, forming three distinct phyletic lineages within the genus. The strains grew best with 5–30 g NaCl l−1 at 20–30 °C. DNA G+C contents for strains MCS24T, FWC40T and FWC43T were between 65 and 67 mol%, in accordance with values reported previously for other species of the genus. Moreover, chemotaxonomic data and physiological and biochemical tests allowed the phenotypic differentiation of three novel species within the genus Brevundimonas, for which the names Brevundimonas halotolerans sp. nov. (type strain MCS24T =LMG 25346T =CCUG 58273T), Brevundimonas poindexterae sp. nov. (type strain FWC40T =LMG 25261T =CCUG 57883T) and Brevundimonas staleyi sp. nov. (type strain FWC43T =LMG 25262T =CCUG 57884T) are proposed.

scholarly journals Halalkalicoccus jeotgali sp. nov., a halophilic archaeon from shrimp jeotgal, a traditional Korean fermented seafood

2007 ◽  
Vol 57 (10) ◽  
pp. 2296-2298 ◽  
Author(s):  
Seong Woon Roh ◽  
Young-Do Nam ◽  
Ho-Won Chang ◽  
Youlboong Sung ◽  
Kyoung-Ho Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
Gene Sequences ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Extremely Halophilic Archaeon ◽  
Physiological And Biochemical

A novel, extremely halophilic archaeon B3T was isolated from shrimp-salted seafood. Its morphology, physiology, biochemical features and 16S rRNA gene sequence were characterized. Strain B3T is non-motile, Gram-variable, requires at least 10 % (w/v) NaCl for growth and grows in the ranges of 21–50 °C and pH 6.5–9.0. The DNA G+C content of strain B3T was 63.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain B3T belonged to the genus Halalkalicoccus and was phylogenetically closely related to the type strain Halalkalicoccus tibetensis (98.64 %). However, DNA–DNA hybridization experiments showed 7.0 % relatedness between strain B3T and a strain of a reference species of the genus Halalkalicoccus. Combined analysis of 16S rRNA gene sequences, DNA–DNA relatedness data, physiological and biochemical tests indicated that the genotypic and phenotypic characteristics differentiate strain B3T from other Halalkalicoccus species. On the basis of the evidence presented in this report, strain B3T represents a novel species of the genus Halalkalicoccus, for which the name Halalkalicoccus jeotgali. sp. nov. is proposed. The type strain is B3T (=KCTC 4019T=DSM 18796T=JCM 14584T=CECT 7217T).

scholarly journals Halorubrum ezzemoulense sp. nov., a halophilic archaeon isolated from Ezzemoul sabkha, Algeria

2006 ◽  
Vol 56 (7) ◽  
pp. 1583-1588 ◽  
Author(s):  
Karima Kharroub ◽  
Teresa Quesada ◽  
Raquel Ferrer ◽  
Susana Fuentes ◽  
Margarita Aguilera ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Genomic Dna ◽  
Type Strain ◽  
Rrna Gene ◽  
Halophilic Archaeon ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Extremely Halophilic Archaeon ◽  
Derivatives Of

A novel extremely halophilic archaeon was isolated from Ezzemoul sabkha, Algeria. The strain, designated 5.1T, was neutrophilic, motile and Gram-negative. At least 15 % (w/v) NaCl was required for growth. The isolate grew at pH 6.5–9.0, with optimum growth at pH 7.0–7.5. Mg2+ was required for growth. Polar lipids were C20C20 derivatives of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, and phosphatidylglycerol sulfate and sulfated diglycosyl diether. The genomic DNA G+C content of strain 5.1T was 61.9 mol% (T m). Phylogenetic analysis based on comparison of 16S rRNA gene sequences revealed that strain 5.1T clustered with Halorubrum species. The results of DNA–DNA hybridization and biochemical tests allowed genotypic and phenotypic differentiation of strain 5.1T from other Halorubrum species. The name Halorubrum ezzemoulense sp. nov. (type strain 5.1T=CECT 7099T=DSM 17463T) is proposed.

scholarly journals Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens

2007 ◽  
Vol 57 (11) ◽  
pp. 2513-2518 ◽  
Author(s):  
Peter Kämpfer ◽  
Holger C. Scholz ◽  
Birgit Huber ◽  
Enevold Falsen ◽  
Hans-Jürgen Busse
Keyword(s):  
Type Strain ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Clinical Specimens ◽  
Dna Reassociation ◽  
Spore Forming Bacteria ◽  
Physiological And Biochemical ◽  
Sequence Similarities ◽  
Ochrobactrum Pseudogrignonense

Three Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. On the basis of 16S rRNA gene sequence similarities, these strains (CCUG 30717T, CCUG 43892 and CCUG 38531T) were shown to belong to the Alphaproteobacteria, most closely related to Ochrobactrum grignonense (99.0 and 98.2 % similarity to the type strain). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids C18 : 1 ω7c and C19 : 0 cyclo ω8c) supported the affiliation of the isolates to the genus Ochrobactrum. The results of DNA–DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from described Ochrobactrum species. Isolates CCUG 30717T and CCUG 43892 were closely related on the basis of DNA–DNA reassociation experiments and therefore represent one novel species, for which the name Ochrobactrum pseudogrignonense sp. nov. is proposed, with the type strain CCUG 30717T (=CIP 109451T). Isolate CCUG 38531T was different from these strains and also from other Ochrobactrum species. For this strain, the name Ochrobactrum haematophilum sp. nov. is proposed, with the type strain CCUG 38531T (=CIP 109452T).

Deefgea chitinilytica sp. nov., isolated from a wetland

2010 ◽  
Vol 60 (6) ◽  
pp. 1450-1453 ◽  
Author(s):  
Wen-Ming Chen ◽  
Yu-Ni Chung ◽  
Tzu-Fang Chiu ◽  
Chih-Yu Cheng ◽  
A. B. Arun ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Analyses ◽  
Physiological Data ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gene Sequence Analysis ◽  
Taxonomic Approach ◽  
Physiological And Biochemical

A bacterial strain, designated Nsw-4T, was isolated from a water sample of Niao-Song Wetland Park in Taiwan and was characterized by using a polyphasic taxonomic approach. Strain Nsw-4T was Gram-negative, aerobic, ivory-coloured, rod-shaped and motile by means of a polar flagellum. Growth occurred at 15–37 °C, pH 6.0–8.0 and 0–2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain belonged to the genus Deefgea and that its closest neighbour was Deefgea rivuli WB 3.4-79T (96.9 %). The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from D. rivuli WB 3.4-79T. The major fatty acids were C16 : 1ω7c and C16 : 0. The G+C content of the genomic DNA was 53.7 mol%. On the basis of 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain Nsw-4T should be classified as representing a novel species and the second member of the genus Deefgea, for which the name Deefgea chitinilytica sp. nov. is proposed. The type strain is Nsw-4T (=BCRC 17934T=LMG 24817T).

scholarly journals Actinophytocola burenkhanensis sp. nov., isolated from Mongolian soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1033-1038 ◽  
Author(s):  
Ismet Ara ◽  
Baljinova Tsetseg ◽  
Damdinsuren Daram ◽  
Manabu Suto ◽  
Katsuhiko Ando
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Actinomycete Strain ◽  
Physiological And Biochemical

A Gram-positive, aerobic, non-motile actinomycete, strain MN08-A0203T, that formed pale yellow to orange-brown colonies and non-fragmented branched substrate mycelium is described. The strain, which produced very scanty aerial mycelium-like structures and scanty formation of spherical bodies on the aerial mycelium on Bennett’s agar medium, was studied in detail to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity studies, strain MN08-A0203T grouped with the genus Actinophytocola, being most closely related to the type strain of Actinophytocola oryzae (97.8 %). Chemotaxonomic data [menaquinone MK-9(H4); iso-C16 : 0 (27 %), iso-C15 : 0 (18 %), C16 : 1 H (8 %), C16 : 0 9-methyl (8 %) as major fatty acids; glucose, galactose, ribose, arabinose, mannose, rhamnose and xylose (trace) as whole cell sugars; diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and ninhydrin-positive phosphoglycolipids as polar phospholipids] supported allocation of the strain to the genus Actinophytocola. Furthermore, the results of DNA–DNA hybridization of strain MN08-A0203T with the type strain of Actinophytocola oryzae revealed that the two strains were genetically distinct from each other. Moreover, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain MN08-A0203T from closely related species. Thus, MN08-A0203T represents a novel species of the genus Actinophytocola, for which the name Actinophytocola burenkhanensis sp. nov. is proposed, with MN08-A0203T ( = NBRC 105883T  = VTCC D9-23T) as the type strain.

scholarly journals Enterorhabdus caecimuris sp. nov., a member of the family Coriobacteriaceae isolated from a mouse model of spontaneous colitis, and emended description of the genus Enterorhabdus Clavel et al. 2009

2010 ◽  
Vol 60 (7) ◽  
pp. 1527-1531 ◽  
Author(s):  
Thomas Clavel ◽  
Wayne Duck ◽  
Cédric Charrier ◽  
Mareike Wenning ◽  
Charles Elson ◽  
...  
Keyword(s):  
Mouse Model ◽  
Type Strain ◽  
Intestinal Inflammation ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Physiological And Biochemical

The C3H/HeJBir mouse model of intestinal inflammation was used for isolation of a Gram-positive, rod-shaped, non-spore-forming bacterium (B7T) from caecal suspensions. On the basis of partial 16S rRNA gene sequence analysis, strain B7T was a member of the class Actinobacteria, family Coriobacteriaceae, and was related closely to Enterorhabdus mucosicola Mt1B8T (97.6 %). The major fatty acid of strain B7T was C16 : 0 (19.1 %) and the respiratory quinones were mono- and dimethylated. Cells were aerotolerant, but grew only under anoxic conditions. Strain B7T did not convert the isoflavone daidzein and was resistant to cefotaxime. The results of DNA–DNA hybridization experiments and additional physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain B7T from the type strain of E. mucosicola. Therefore, strain B7T represents a novel species, for which the name Enterorhabdus caecimuris sp. nov. is proposed. The type strain is B7T (=DSM 21839T =CCUG 56815T).

scholarly journals Halomonas vilamensis sp. nov., isolated from high-altitude Andean lakes

2011 ◽  
Vol 61 (5) ◽  
pp. 1211-1217 ◽  
Author(s):  
Rodolfo Javier Menes ◽  
Claudia Elizabeth Viera ◽  
María Eugenia Farías ◽  
Manfredo J. Seufferheld
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Limited Range ◽  
Hypersaline Lake ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Closely Related Species ◽  
Phenotypic Differentiation ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene

A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (SV325T) was isolated from the sediment of a hypersaline lake located 4600 m above sea level (Laguna Vilama, Argentina). Strain SV325T formed cream to pink colonies, was motile and moderately halophilic, and tolerated NaCl concentrations of 1–25 % (w/v) with an optimum of 5–10 % (w/v). Growth occurred at 5–40 °C (optimum around 30 °C) and at pH 5.0–10.0 (optimum 7.0–8.0). The bacterium did not produce exopolysaccharides and stained positively for intracellular polyphosphate granules but not for poly-β-hydroxyalkanoates. It produced catalase and oxidase, reduced nitrate to nitrite, hydrolysed gelatin, did not produce acids from sugars and utilized a limited range of substrates as carbon and energy sources: acetate, caproate, fumarate, dl-β-hydroxybutyrate, malate, maleate, malonate and succinate. The predominant ubiquinones were Q-9 (92.5 %) and Q-8 (7.5 %), the major fatty acids were C19 : 0 cyclo ω8c, C16 : 0, C17 : 0 cyclo and C16 : 1ω7c/iso-C15:0 2-OH, and the DNA G+C content was 55.0 mol%. Phylogenetic analyses based on the 16S rRNA gene indicated that strain SV325T belongs to the genus Halomonas in the class Gammaproteobacteria. Physiological and biochemical tests allowed phenotypic differentiation of strain SV325T from closely related species with validly published names. We therefore propose a novel species, Halomonas vilamensis sp. nov., with type strain SV325T ( = DSM 21020T  = LMG 24332T).

scholarly journals Rhodovulum visakhapatnamense sp. nov.

2007 ◽  
Vol 57 (8) ◽  
pp. 1762-1764 ◽  
Author(s):  
T. N. R. Srinivas ◽  
P. Anil Kumar ◽  
Ch. Sasikala ◽  
Ch. V. Ramana ◽  
J. F. Imhoff
Keyword(s):  
Dna Hybridization ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Rhodovulum Sulfidophilum ◽  
Physiological And Biochemical

A Gram-negative, rod-shaped, phototrophic bacterium (JA181T) was isolated from a tidal water sample. On the basis of 16S rRNA gene sequence similarity, strain JA181T was shown to belong to the class Alphaproteobacteria, most closely related to Rhodovulum sulfidophilum (97.8 % similarity to the type strain), Rhodovulum adriaticum (93 %), Rhodovulum robiginosum (93 %), Rhodovulum iodosum (94 %), Rhodovulum imhoffii (94 %), Rhodovulum strictum (95 %), Rhodovulum euryhalinum (94.6 %) and Rhodovulum marinum (94.6 %). DNA–DNA hybridization with Rdv. sulfidophilum DSM 1374T (relatedness of 39 % with strain JA181T) and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain JA181T from the eight Rhodovulum species with validly published names. Strain JA181T therefore represents a novel species, for which the name Rhodovulum visakhapatnamense sp. nov. is proposed (type strain JA181T =JCM 13531T =ATCC BAA-1274T =DSM 17937T).

scholarly journals Endozoicomonas montiporae sp. nov., isolated from the encrusting pore coral Montipora aequituberculata

2010 ◽  
Vol 60 (5) ◽  
pp. 1158-1162 ◽  
Author(s):  
Cho-Song Yang ◽  
Ming-Hui Chen ◽  
A. B. Arun ◽  
Chaolun Allen Chen ◽  
Jih-Terng Wang ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Optimal Growth ◽  
Rrna Gene ◽  
The Novel ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
Gene Sequence Analysis ◽  
Southern Taiwan ◽  
Physiological And Biochemical

A Gram-negative, aerobic, rod-shaped bacterium, designated strain CL-33T, was isolated from the encrusting pore coral Montipora aequituberculata collected from seawater off the coast of southern Taiwan. 16S rRNA gene sequence analysis showed that the strain clustered closely with Endozoicomonas elysicola MKT110T (96.7 % similarity). The novel strain required NaCl for growth and exhibited optimal growth at 25 °C and in the presence of 2–3 % NaCl. Predominant cellular fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 39.6 %), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c; 32.8 %) and C16 : 0 (12.0 %). The DNA G+C content of strain CL-33T was 50.0 mol%. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from E. elysicola. It is evident from the genotypic, phenotypic and chemotaxonomic data presented that strain CL-33T represents a novel species of the genus Endozoicomonas, for which the name Endozoicomonas montiporae sp. nov. is proposed. The type strain is CL-33T (=LMG 24815T =BCRC 17933T).

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