scholarly journals Litoribacter ruber gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a soda lake sediment

2010 ◽  
Vol 60 (12) ◽  
pp. 2996-3001 ◽  
Author(s):  
Shi-Ping Tian ◽  
Yong-Xia Wang ◽  
Bin Hu ◽  
Xiao-Xia Zhang ◽  
Wei Xiao ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Soda Lake ◽  
Optimal Growth ◽  
Growth Conditions ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Sequence Comparisons ◽  
Respiratory Quinone ◽  
Major Respiratory Quinone ◽  
Respective Type

A novel alkaliphilic, halotolerant, rod-shaped bacterium, designated strain YIM CH208T, was isolated from a soda lake in Yunnan, south-west China. The taxonomy of strain YIM CH208T was investigated by a polyphasic approach. Strain YIM CH208T was Gram-negative, strictly aerobic and non-motile and formed red colonies. Optimal growth conditions were 28 °C, pH 8.5 and 0.5–2.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct line within a clade containing the genus Echinicola in the phylum Bacteroidetes and was related to the species Echinicola pacifica and Rhodonellum psychrophilum, with sequence similarity of 91.7 and 91.6 % to the respective type strains. The DNA G+C content was 45.1 mol%. The major respiratory quinone was menaquinone-7 (MK-7). The predominant cellular fatty acids were iso-C17 : 1 ω9c (19.9 %), C15 : 0 3-OH (12.1 %), iso-C17 : 0 3-OH (11.3 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 10.7 %) and C17 : 1 ω6c (8.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain YIM CH208T represents a novel species of a new genus, for which the name Litoribacter ruber gen. nov., sp. nov. is proposed. The type strain of Litoribacter ruber is YIM CH208T (=ACCC 05414T =KCTC 22899T).

Flavobacterium chungbukense sp. nov., isolated from soil

2011 ◽  
Vol 61 (11) ◽  
pp. 2734-2739 ◽  
Author(s):  
Chae-Sung Lim ◽  
Yong-Sik Oh ◽  
Jae-Kwan Lee ◽  
A-Rum Park ◽  
Jae-Soo Yoo ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Flavobacterium Johnsoniae ◽  
The Family ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100T, was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953T (97.6 %) and Flavobacterium johnsoniae KACC 11410T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5–97.0 %. Growth occurred at 4–30 °C, at pH 5.0–9.0 and in the presence of 0–2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C15 : 0 (17.3 %), summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 15.5 %) and C16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA–DNA relatedness was 3.0 % with F. glaciei JCM 13953T and 11.5 % with F. johnsoniae KACC 11410T. On the basis of the evidence from this study, strain CS100T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100T ( = KACC 15048T = JCM 17386T).

scholarly journals Salinicoccus qingdaonensis sp. nov., isolated from coastal seawater during a bloom of green algae

2012 ◽  
Vol 62 (Pt_3) ◽  
pp. 545-549 ◽  
Author(s):  
Zhe Qu ◽  
Zhao Li ◽  
Xiuming Zhang ◽  
Xiao-Hua Zhang
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Dna Relatedness ◽  
Green Algal ◽  
Pr China ◽  
Major Respiratory Quinone ◽  
Type Strains ◽  
The 16S Rrna Gene

A novel Gram-stain-positive, white-pigmented, non-motile, non-sporulating, catalase- and oxidase-positive, strictly aerobic coccus, designated strain ZXM223T, was isolated from a seawater sample collected from the coast of Qingdao, PR China, during a green algal bloom. It grew at pH 6.0–10.5 and 0–25.0 % (w/v) NaCl, with optimum growth at pH 8.5 and 3.0 % (w/v) NaCl. Growth occurred at 16–42 °C (optimum at 28 °C). The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Menaquinone 6 (MK-6) was the major respiratory quinone. The polar lipids were phosphatidylglycerol, three unidentified phospholipids and two unknown glycolipids. The peptidoglycan type was l-Lys–Gly5–6. The genomic DNA G+C content was 43.5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain ZXM223T within the genus Salinicoccus, with sequence similarity of 92.2–97.1 % between ZXM223T and the type strains of this genus. The closest relatives were Salinicoccus kunmingensis YIM Y15T, ‘S. salitudinis’ YIM-C678 and S. alkaliphilus T8T. The DNA–DNA relatedness between strain ZXM223T and S. kunmingensis CGMCC 1.6302T and ‘S. salitudinis’ CGMCC 1.6299 ( = YIM-C678) was 37±3 and 30±2 %, respectively. The phenotypic, chemotaxonomic and phylogenetic characteristics and low DNA–DNA relatedness support the proposal of a novel species of the genus Salinicoccus, Salinicoccus qingdaonensis sp. nov., with the type strain ZXM223T ( = LMG 24855T  = CGMCC 1.8895T).

scholarly journals Amphiplicatus metriothermophilus gen. nov., sp. nov., a thermotolerant alphaproteobacterium isolated from a hot spring

2014 ◽  
Vol 64 (Pt_8) ◽  
pp. 2805-2811 ◽  
Author(s):  
Zhang Zhen-Li ◽  
Zhang Xin-Qi ◽  
Wu Nan ◽  
Zhang Wen-Wu ◽  
Zhu Xu-Fen ◽  
...  
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Aerobic Bacterium ◽  
Respiratory Quinone ◽  
Content Type ◽  
North West ◽  
Link Type ◽  
Major Respiratory Quinone

A thermotolerant, Gram-strain-negative, non-spore-forming and strictly aerobic bacterium, designated GU51T, was isolated from Guhai hot spring in Jimsar county, Xinjiang province, north-west China. Each cell of strain GU51T consisted of an oval body and two symmetrical long (3–6 µm) prosthecae. The strain moved by polar flagellum. Oxidase and catalase were produced. Strain GU51T grew within the ranges of 37–65 °C (optimum 48–50 °C), 0.5–7.5 % (w/v) NaCl (optimum 2–3 %) and pH 6.0–9.0 (optimum pH 7.5). The major respiratory quinone detected was ubiquinone 10 (U-10) and the genomic DNA G+C content was 66.7±0.4 mol%. Major fatty acids (>5 %) were C16 : 0, C18 : 1ω7c and 11-methyl C18 : 1ω7c. The polar lipids consisted of diphosphatidylglycerol, five glycolipids, phosphatidylglycerol and an unknown phospholipid. Phylogenetic analysis showed the closest relatives of strain GU51T were members of the genus Parvularcula with 92.3 % 16S rRNA gene sequence similarity. On the basis of this polyphasic taxonomic characterization, it is suggested that strain GU51T represents a novel species of a new genus in the family ‘ Parvularculaceae ’, for which the name Amphiplicatus metriothermophilus gen. nov., sp. nov. is proposed. The type strain of the type species is GU51T ( = CGMCC 1.12710T = JCM 19779T).

Flavobacterium compostarboris sp. nov., isolated from leaf-and-branch compost, and emended descriptions of Flavobacterium hercynium , Flavobacterium resistens and Flavobacterium johnsoniae

2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 2018-2024 ◽  
Author(s):  
Joong-Jae Kim ◽  
Eiko Kanaya ◽  
Hang-Yeon Weon ◽  
Yuichi Koga ◽  
Kazufumi Takano ◽  
...  
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Major Polar Lipid ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Flavobacterium Johnsoniae ◽  
Major Respiratory Quinone

A strictly aerobic, Gram-negative, yellow-pigmented, non-spore-forming rod, designated 15C3T, was isolated from aerobic leaf-and-branch compost at EXPO Park in Osaka, Japan. Growth was observed at 9–33 °C (optimum 25 °C) and pH 5.6–7.9 (optimum pH 6.1–7.0). No growth occurred with >2 % (w/v) NaCl. Strain 15C3T reduced nitrate to nitrogen and showed catalase activity but not oxidase activity. The predominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH). The isolate contained phosphatidylethanolamine as the major polar lipid and menaquinone-6 as the major respiratory quinone. The G+C content of the genomic DNA of strain 15C3T was 33.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 15C3T belonged to the genus Flavobacterium and was most closely related to Flavobacterium hercynium WB 4.2-33T (96.9 % sequence similarity). On the basis of phenotypic and phylogenetic distinctiveness, strain 15C3T is considered to represent a novel species in the genus Flavobacterium , for which the name Flavobacterium compostarboris sp. nov. is proposed. The type strain is 15C3T ( = KACC 14224T  = JCM 16527T). Emended descriptions of F. hercynium , Flavobacterium resistens and Flavobacterium johnsoniae are also given.

scholarly journals Algoriphagus jejuensis sp. nov., isolated from seawater

2012 ◽  
Vol 62 (2) ◽  
pp. 409-413 ◽  
Author(s):  
Dong-Heon Lee ◽  
Hyung-Yeel Kahng ◽  
Sun Bok Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
High Sequence Similarity ◽  
Respiratory Quinone ◽  
Distinct Lineage ◽  
Major Respiratory Quinone

A Gram-negative, pink-pigmented, non-motile, strictly aerobic rod, designated CNU040T, was isolated from seawater from the coast of Jeju Island in Korea. The temperature, pH and NaCl ranges for growth were 4–30 °C, pH 5.5–10.0 and 0–5.0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CNU040T belonged to a distinct lineage in the genus Algoriphagus and exhibited high sequence similarity with Algoriphagus terrigena DS-44T (98.3 %) and Algoriphagus alkaliphilus AC-74T (96.6 %) and lower sequence similarity (<96.0 %) with all other members of the genus Algoriphagus. DNA–DNA relatedness between strain CNU040T and A. terrigena KCTC 12545T was 44.5 %. The DNA G+C content of the isolate was 48.5 mol% and the major respiratory quinone was menaquinone-7. The major cellular fatty acids were iso-C15 : 0 (28.6 %) and summed feature 3 (consisting of iso-C15 : 0 2-OH and/or C16 : 1ω7c; 24.0 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, one unknown amino lipid, one unknown aminophospholipid and three unknown polar lipids. On the basis of phenotypic, phylogenetic and genotypic data, strain CNU040T represents a novel species within the genus Algoriphagus, for which the name Algoriphagus jejuensis sp. nov. is proposed. The type strain is CNU040T ( = KCTC 22647T  = JCM 16112T).

scholarly journals Luteimonas soli sp. nov., isolated from farmland soil

2015 ◽  
Vol 65 (Pt_12) ◽  
pp. 4809-4815 ◽  
Author(s):  
Xiang Wang ◽  
Hong-Xing Yang ◽  
Ying-Kun Zhang ◽  
Shi-Jun Zhu ◽  
Xiao-Wei Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Respiratory Quinone ◽  
Farmland Soil ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene

A yellow-pigmented bacterial strain, designated Y2T, was isolated from farmland soil in Bengbu, Anhui province, China. Cells of strain Y2T were Gram-stain-negative, strictly aerobic, non-motile and rod-shaped. Strain Y2T grew optimally at pH 7.0, 30 °C and in the presence of 2 % (w/v) NaCl. The DNA G+C content was 68.9 mol%. The major fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0, summed feature 9 (C16 : 0 10-methyl and/or iso-C17 : 1ω9c), iso-C11 : 0 3-OH and iso-C11 : 0. The major respiratory quinone was ubiquinone-8 (Q-8), and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain Y2T was most closely related to Luteimonas mephitis B1953/27.1T (99.1 % 16S rRNA gene sequence similarity), followed by Luteimonas lutimaris G3T (98.6 %), Luteimonas abyssi XH031T (96.2 %) and Luteimonas aquatica RIB1-20T (96.0 %). Strain Y2T exhibited low DNA–DNA relatedness with Luteimonas mephitis B1953/27.1T (43.6 ± 0.5 %) and Luteimonas lutimaris G3T (43.9 ± 2.1 %). On the basis of phenotypic, genotypic and phylogenetic evidence, strain Y2T represents a novel species of the genus Luteimonas, for which the name Luteimonas soli sp. nov. is proposed. The type strain is Y2T ( = ACCC 19799T = KCTC 42441T).

Leucothrix pacifica sp. nov., isolated from seawater, and emended description of the genus Leucothrix

2015 ◽  
Vol 65 (Pt_8) ◽  
pp. 2397-2402 ◽  
Author(s):  
Zenghu Zhang ◽  
Xin Gao ◽  
Long Wang ◽  
Xiao-Hua Zhang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Ocean Drilling Program ◽  
Respiratory Quinone ◽  
Integrated Ocean Drilling Program ◽  
South Pacific Gyre

A Gram-stain-negative, strictly aerobic, non-flagellated, non-gliding, oxidase- and catalase-positive, white-pigmented and rod-shaped bacterium, designated strain XH122T, was isolated from a surface seawater sample collected from the South Pacific Gyre (45° 58′ E 163° 11′ S) during Integrated Ocean Drilling Program Expedition 329.Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain XH122T belonged to the genus Leucothrix and showed highest 16S rRNA gene sequence similarity to Leucothrix mucor DSM 2157T (94.3 %). It showed lower sequence similarities ( < 90.7 %) with all other representatives of the class Gammaproteobacteria. Optimal growth occurred in the presence of 2 % (w/v) NaCl, at pH 8.0 and at 28 °C. The DNA G+C content of strain XH122T was 46.2 mol%. The major fatty acids were C16 : 0, C16 : 1ω9c and C18 : 1ω9c. The major respiratory quinone was ubiquinone-8 (Q-8). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. On the basis of data from this polyphasic study, strain XH122T is considered to represent a novel species of the genus Leucothrix, for which the name Leucothrix pacifica sp. nov. is proposed. The type strain is XH122T ( = DSM 25984T = JCM 18388T).

scholarly journals Niastella populi sp. nov., isolated from soil of Euphrates poplar (Populus euphratica) forest, and emended description of the genus Niastella

2010 ◽  
Vol 60 (3) ◽  
pp. 542-545 ◽  
Author(s):  
Kundi Zhang ◽  
Yang Wang ◽  
Yali Tang ◽  
Jun Dai ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Populus Euphratica ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Optimum Ph ◽  
Emended Description ◽  
Euphrates Poplar ◽  
Major Respiratory Quinone

A novel bacterial strain, designated THYL-44T, was isolated from the soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China. The cells were strictly aerobic, Gram-staining-negative, non-flagellated, non-motile and filamentous. Growth occurred at 17–37 °C (optimum 30 °C), at pH 5.0–8.0 (optimum pH 7.0) and with 0–1 % NaCl (w/v; optimum 0 %). Flexirubin pigments were not produced. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain THYL-44T was closely related to Niastella koreensis KACC 11465T (95.5 % sequence similarity). The major respiratory quinone was MK-7 and the predominant cellular fatty acids were iso-C15 : 0 (28.6 %), iso-C17 : 0 3-OH (23.9 %) and iso-C15 : 1 G (17.4 %). The DNA G+C content was 45.2 mol%. Therefore, the phylogenetic, physiological and chemotaxonomic data demonstrated that strain THYL-44T represents a novel species of the genus Niastella, for which the name Niastella populi sp. nov. is proposed. The type strain is THYL-44T (=CCTCC AB 208238T=KCTC 22560T). On the basis of new data, an emended description of the genus Niastella is also proposed.

Rhodocytophaga aerolata gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from air

2010 ◽  
Vol 60 (7) ◽  
pp. 1554-1558 ◽  
Author(s):  
Rangasamy Anandham ◽  
Hang-Yeon Weon ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
Soon-Wo Kwon
Keyword(s):  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Polar Lipids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
The Family ◽  
Major Respiratory Quinone

A strictly aerobic, Gram-staining-negative, oxidase- and catalase-positive, non-motile, rod-shaped bacterium, designated strain 5416T-29T, was isolated from air and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Flexirubin-type pigments were absent. The strain formed a distinct phylogenetic lineage within the family Cytophagaceae of the phylum Bacteroidetes. Strain 5416T-29T did not show more than 88 % 16S rRNA gene sequence similarity to the type strain of any recognized species. The major cellular fatty acids were C16 : 1 ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. The polar lipids were phosphatidylethanolamine, one unknown amino lipid and several unknown polar lipids. Menaquinone-7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain 5416T-29T was 45.5 mol%. Results of phenotypic and phylogenetic analyses clearly indicate that strain 5416T-29T represents a novel species of a new genus in the family Cytophagaceae, for which the name Rhodocytophaga aerolata gen. nov., sp. nov. is proposed. The type strain of Rhodocytophaga aerolata is 5416T-29T (=KACC 12507T =DSM 22190T).

scholarly journals Formosa spongicola sp. nov., isolated from the marine sponge Hymeniacidon flavia

2011 ◽  
Vol 61 (2) ◽  
pp. 330-333 ◽  
Author(s):  
Byoung-Jun Yoon ◽  
Duck-Chul Oh
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Marine Sponge ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
The Family ◽  
Major Respiratory Quinone

A Gram-negative, yellow-pigmented, rod-shaped, strictly aerobic, non-flagellated, oxidase- and catalase-positive, marine bacterium, designated A2T, was isolated from a marine sponge, Hymeniacidon flavia, collected from the coast of Jeju Island, South Korea. Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that strain A2T was a member of the family Flavobacteriaceae. Its closest relatives were Formosa agariphila KMM 3901T and Formosa algae KMM 3553T (96.99 and 96.98 % 16S rRNA gene sequence similarity, respectively). DNA–DNA relatedness between strain A2T and F. agariphila KMM 3901T and F. algae KMM 3553T was 14.1 and 26.8 %, respectively. The dominant fatty acids (>5 %) of strain A2T were iso-C15 : 0 (33.9 %), iso-C17 : 0 3-OH (20.8 %), iso-C15 : 1 G (10.5 %) and iso-C15 : 0 3-OH (6.1 %). The DNA G+C content of strain A2T was 36.0 mol% and the major respiratory quinone was MK-6. On the basis of phenotypic and phylogenetic analysis, strain A2T represents a novel species of the genus Formosa, for which the name Formosa spongicola sp. nov. is proposed. The type strain is A2T (=KCTC 22662T =DSM 22637T).

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