scholarly journals Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China

2006 ◽  
Vol 56 (6) ◽  
pp. 1217-1221 ◽  
Author(s):  
Yanfen Xue ◽  
Xinqi Zhang ◽  
Cheng Zhou ◽  
Yueju Zhao ◽  
Don A. Cowan ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Hot Spring ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Positive Bacterium ◽  
The Family ◽  
Novel Genus And Species ◽  
Distinct Lineage ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A thermophilic, alkaliphilic and catalase-positive bacterium, designated strain HA6T, was isolated from a hot spring in China. The strain was aerobic and chemo-organotrophic and grew optimally at 60 °C, pH 8.5 and 1.5 % (w/v) NaCl. The cells were Gram-positive rods, forming single terminal endospores. The predominant cellular fatty acids were iso-C15 : 0 and iso-C17 : 0. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The genomic DNA G+C content was 45.2 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain HA6T formed a distinct lineage within the family Bacillaceae and was most closely related to Bacillus horti K13T and Bacillus smithii DSM 4216T, with sequence similarities of 91.8 and 93.1 %, respectively. On the basis of its physiological and molecular properties, strain HA6T should be placed in a novel genus and species, for which the name Caldalkalibacillus thermarum gen. nov., sp. nov. is proposed. The type strain of Caldalkalibacillus thermarum is strain HA6T (=CGMCC 1.4242T=JCM 13486T).

scholarly journals Maribacter polysiphoniae sp. nov., isolated from a red alga

2007 ◽  
Vol 57 (12) ◽  
pp. 2840-2843 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Marc Vancanneyt ◽  
Paul De Vos ◽  
Seung Bum Kim ◽  
Myung Sook Lee ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Novel Species ◽  
Red Alga ◽  
Rrna Gene ◽  
Positive Bacterium ◽  
The Pacific ◽  
Yellow Orange ◽  
Distinct Lineage ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A novel gliding, heterotrophic, Gram-negative, yellow–orange-pigmented, aerobic, oxidase- and catalase-positive bacterium, designated strain KMM 6151T, was isolated from the Pacific red alga Polysiphonia japonica. Analysis of the 16S rRNA gene sequence of the strain revealed that it formed a distinct lineage within the genus Maribacter, family Flavobacteriaceae, with sequence similarities in the range 94.6–96.9 %. On the basis of phenotypic, genotypic and phylogenetic data, strain KMM 6151T represents a novel species of the genus Maribacter, for which the name Maribacter polysiphoniae sp. nov. is proposed. The type strain is KMM 6151T (=KCTC 22021T=LMG 23671T).

scholarly journals Lapillicoccus jejuensis gen. nov., sp. nov., a novel actinobacterium of the family Intrasporangiaceae, isolated from stone

2007 ◽  
Vol 57 (12) ◽  
pp. 2794-2798 ◽  
Author(s):  
Soon Dong Lee ◽  
Dong Wan Lee
Keyword(s):  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Straight Chain ◽  
Polar Lipid Profile ◽  
The Family ◽  
Small Stone ◽  
Novel Genus And Species ◽  
Diamino Acid ◽  
The 16S Rrna Gene

A novel, yellow-pigmented actinobacterium was isolated from a small stone collected in Jeju, Republic of Korea. The cells of the organism, designated strain R-Ac013T, were Gram-positive, aerobic, non-motile cocci that occurred singly or in pairs. The strain showed growth at 10–37 °C and pH 4.1–11.1, and tolerated 2 % NaCl. On the basis of the 16S rRNA gene sequence, the organism was phylogenetically related to members of the genera Tetrasphaera (94.6–97.1 % sequence similarity), Terrabacter (96.5 %), Knoellia (96.4 %), Terracoccus (96.4 %), Oryzihumus (96.4 %), Janibacter (96.1–96.4 %) and Intrasporangium (96.2 %). The chemotaxonomic results for the organism were as follows: ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan, acetyl-type murein, MK-8(H4) as the major menaquinone, a DNA G+C content of 74.1 mol%, and a polar lipid profile that comprised diphosphatidylglycerol and phosphatidylinositol. The fatty acid profile consisted of iso- and anteiso-methyl-branched, straight-chain saturated and monounsaturated types, the major components being iso-C16 : 0, C17 : 1 ω8c and iso-C15 : 0. The combination of the phenotypic and phylogenetic data revealed that this strain represents a novel genus and species of the family Intrasporangiaceae, for which the name Lapillicoccus jejuensis gen. nov., sp. nov. is proposed. The type strain is strain R-Ac013T (=KCTC 19200T=DSM 18607T).

Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4149-4157 ◽  
Author(s):  
Nathaniel A. Losey ◽  
Bradley S. Stevenson ◽  
Hans-Jürgen Busse ◽  
Jaap S. Sinninghe Damsté ◽  
W. Irene C. Rijpstra ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Hot Springs ◽  
Hot Spring ◽  
Rrna Gene ◽  
Strictly Anaerobic ◽  
Content Type ◽  
Novel Bacterium ◽  
Fermentative Growth ◽  
Novel Genus And Species ◽  
The 16S Rrna Gene

A novel bacterium was isolated from a freshwater hot spring, the Hale House Spring, located at Hot Springs National Park, Hot Springs, AR, USA. Cells of strain MP-01T stained Gram-negative, were rod-shaped, non-motile, strictly anaerobic and chemo-organotrophic and did not form spores. Growth occurred at 50–65 °C, with an optimum at 60 °C, at pH 6.0–8.0, with an optimum at pH 6.5–7.0, and at NaCl concentrations up to 0.5 % (w/v), with optimum growth in the absence of NaCl. Strain MP-01T was capable of fermentative growth on pyruvate or proteinaceous substrates as well as reducing Fe(III) and Mn(IV). Major fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C17 : 0 and iso-C17 : 0. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine and the major isoprenoid quinone was MK-10. In the polyamine pattern, sym-homospermidine was the predominant compound. The DNA G+C content was 62.7 mol%. Analysis of the 16S rRNA gene sequence of the isolate indicated that strain MP-01T represents the first reported cultivated member of subdivision 23 of the Acidobacteria . It is proposed that strain MP-01T represents a novel genus and species, for which the name Thermoanaerobaculum aquaticum gen. nov., sp. nov. is proposed. The type strain of Thermoanaerobaculum aquaticum is MP-01T ( = DSM 24856T = JCM 18256T).

scholarly journals Laetantesicola Maris Gen. Nov., Sp. Nov. Isolated From the Sea of Japan Shallow Sediments.

2021 ◽  
Author(s):  
Lyudmila A. Romanenko ◽  
Valeriya V. Kurilenko ◽  
Nadezhda Yu. Chernysheva ◽  
Liudmila A. Tekutyeva ◽  
Peter V. Velansky ◽  
...  
Keyword(s):  
Sea Of Japan ◽  
Gene Sequence ◽  
Major Fatty Acid ◽  
The Sea Of Japan ◽  
Rrna Gene ◽  
Unidentified Phospholipid ◽  
Gene Sequence Analysis ◽  
The Family ◽  
Novel Genus And Species ◽  
Distinct Lineage

Abstract A Gram-negative, non-motile bacterium КММ 3653T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КММ 3653T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.7%) and Nioella (95.1-94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КММ 3653T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to Vannielia litorea KCTC 32083T. The average nucleotide identity values between strain КММ 3653T and Vannielia litorea KCTC 32083T, Nioella nitratireducens KCTC 32417T, Litoreibacter albidus KMM 3851T, and Pseudoruegeria aquimaris CECT 7680T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КММ 3653T contained Q-10 as the predominant ubiquinone and C18:1ω7c as the major fatty acid followed by C16:0. The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8 % was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, strain КММ 3653T is proposed to be classified as a novel genus and species Laetantesicola maris gen. nov., sp. nov. The type strain of Laetantesicola maris gen. nov., sp. nov. is КММ 3653T (=KCTC 82575T).

scholarly journals Euzebyella saccharophila gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae

2010 ◽  
Vol 60 (12) ◽  
pp. 2871-2876 ◽  
Author(s):  
Teresa Lucena ◽  
Javier Pascual ◽  
Assunta Giordano ◽  
Agata Gambacorta ◽  
Esperanza Garay ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sea Salts ◽  
Respiratory Quinone ◽  
Cellular Fatty Acids ◽  
The Family ◽  
Novel Genus And Species ◽  
The 16S Rrna Gene

Strain 7SM30T , an aerobic marine, Gram-negative, heterotrophic and yellow- to orange-pigmented bacterium isolated from seawater from Castellón, Spain, was characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that the isolate represented a novel lineage within the family Flavobacteriaceae. The most closely related genera were Pseudozobellia, Zobellia and Kriegella. Cells of strain 7SM30T were non-motile rods that required sea salts for growth, used a wide variety of carbohydrates as sole carbon and energy sources and, unlike species of the genera Pseudozobellia and Zobellia, did not possess flexirubin-type pigment or hydrolyse agar. Strain 7SM30T contained MK6 as the sole respiratory quinone. Phosphatidylethanolamine (PE) was the only identifiable polar lipid, although other lipids were also detected. The predominant cellular fatty acids were saturated C15 and monounsaturated C15. The DNA G+C content was around 40 mol%. On the basis of extensive phenotypic and phylogenetic comparative analysis, it is concluded that the new strain represents a novel genus and species, for which the name Euzebyella saccharophila gen. nov., sp. nov., is proposed. The type strain of the type species is 7SM30T (=CECT 7477T=KCTC 22655T).

scholarly journals Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces

2007 ◽  
Vol 57 (11) ◽  
pp. 2694-2698 ◽  
Author(s):  
Yi Jiang ◽  
Shu-Kun Tang ◽  
Jutta Wiese ◽  
Li-Hua Xu ◽  
Johannes F. Imhoff ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Type Ii ◽  
Rrna Gene Sequence ◽  
Genus Streptomyces ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A novel isolate belonging to the genus Streptomyces, strain YIM 47672T, was obtained from soil from Hainan, an island in China. The characterization of this isolate was performed by using a polyphasic approach. The strain formed long spore chains in the aerial mycelia. The cell wall contained l-diaminopimelic acid, traces of meso-diaminopimelic acid and glycine. Whole-cell hydrolysates contained galactose and xylose. The phospholipid was of type II. The 16S rRNA gene sequence similarities for YIM 47672T with respect to the most closely related type strains of species of the genus Streptomyces were less than 96.3 %. Therefore strain YIM 47672T represents a novel member of the genus Streptomyces, for which the name Streptomyces hainanensis sp. nov. is proposed. The type strain is YIM 47672T (=CCTCC AA 205017T=DSM 41900T).

Microlunatus ginsengisoli sp. nov., isolated from soil of a ginseng field

2007 ◽  
Vol 57 (4) ◽  
pp. 713-716 ◽  
Author(s):  
Ying-Shun Cui ◽  
Wan-Taek Im ◽  
Cheng-Ri Yin ◽  
Deok-Chun Yang ◽  
Sung-Taik Lee
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Novel ◽  
Biochemical Tests ◽  
Phenotypic Differentiation ◽  
The Family ◽  
Physiological And Biochemical ◽  
Sequence Similarities

A Gram-positive, aerobic, coccus-shaped, non-endospore-forming bacterium (Gsoil 633T) was isolated from soil from a ginseng field in Pocheon province in South Korea. The novel isolate was characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarities, strain Gsoil 633T was shown to belong to the family Propionibacteriaceae. The closest phylogenetic relative was Microlunatus phosphovorus DSM 19555T, with 96.1 % sequence similarity; the sequence similarity to other members of the family was less than 95.4 %. The isolate was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H4) as the predominant menaquinone and anteiso-C15 : 0, iso-C15 : 0 and iso-C16 : 0 as the major fatty acids. The G+C content of the genomic DNA was 69.8 mol%. The morphological and chemotaxonomic properties of the isolate were consistent with those of M. phosphovorus, but the results of physiological and biochemical tests allowed the phenotypic differentiation of strain Gsoil 633T from this species. Therefore, strain Gsoil 633T represents a novel species, for which the name Microlunatus ginsengisoli sp. nov. is proposed. The type strain is Gsoil 633T (=KCTC 13940T=DSM 17942T).

scholarly journals Alloactinosynnema album gen. nov., sp. nov., a member of the family Actinosynnemataceae isolated from soil

2010 ◽  
Vol 60 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Li-Jie Yuan ◽  
Yu-Qin Zhang ◽  
Li-Yan Yu ◽  
Hong-Yu Liu ◽  
Yan Guan ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Family ◽  
Distinct Lineage ◽  
Diamino Acid ◽  
Signature Pattern ◽  
The 16S Rrna Gene

The taxonomic position of a Gram-stain-positive, aerobic strain, designated 03-9939T, isolated from a soil sample collected from Xinjiang Province, China, was established using a polyphasic approach. Whole-cell hydrolysates of strain 03-9939T contained galactose and ribose as diagnostic sugars and meso-diaminopimelic acid as the diamino acid. The predominant menaquinone was MK-9(H4). The phospholipids consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were iso-C16 : 0 (61.5 %) and iso-C16 : 1 H (11.6 %). The genomic DNA G+C content was 68.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 03-9939T should be placed within the family Actinosynnemataceae, in which the strain formed a distinct lineage. Signature nucleotides in the 16S rRNA gene sequence showed that the strain contained a genus-specific diagnostic nucleotide signature pattern. The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supported the conclusion that strain 03-9939T represents a novel species in a new genus of the family Actinosynnemataceae, for which the name Alloactinosynnema album gen. nov., sp. nov. is proposed. Strain 03-9939T (=DSM 45114T =KCTC 19294T =CCM 7461T) is the type strain of Alloactinosynnema album.

scholarly journals Oryzobacter terrae gen. nov., sp. nov., isolated from paddy soil

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3190-3195 ◽  
Author(s):  
Soo-Jin Kim ◽  
Jun-Muk Lim ◽  
Moriyuki Hamada ◽  
Jae-Hyung Ahn ◽  
Hang-Yeon Weon ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Paddy Soil ◽  
Dna Hybridization ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
The Family ◽  
Total Fatty Acids ◽  
Diamino Acid ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A bacterial strain, PSGM2-16T, was isolated from a pot of paddy soil grown with rice in Suwon region, Republic of Korea, and was characterized as having aerobic, Gram-stain-positive, short-rod-shaped cells with one polar flagellum. The 16S rRNA gene sequence of strain PSGM2-16T revealed the highest sequence similarities with Knoellia locipacati DMZ1T (97.4 %), Fodinibacter luteus YIM C003T (97.2 %) and Lapillicoccus jejuensis R-Ac013T (97.0 %), and the phylogenetic tree showed that strain PSGM2-16T formed a subgroup with Ornithinibacter aureus HB09001T and F. luteus YIM C003T within the family Intrasporangiaceae. The major fatty acids (>10 % of the total fatty acids) of strain PSGM2-16T were iso-C16 : 0, C17 : 1ω8c and iso-C14 : 0. The predominant menaquinone was MK-8(H4). The polar lipids present were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three aminophospholipids and two phospholipids. The peptidoglycan was type A4γ with meso-diaminopimelic acid as the diagnostic diamino acid. DNA–DNA hybridization values between strain PSGM2-16T and closely related taxa were much less than 70 %. The genomic DNA G+C content of strain PSGM2-16T was 70.0 mol%. On the basis of the evidence presented, it is concluded that strain PSGM2-16T represents a novel species of a new genus in the family Intrasporangiaceae, for which the name Oryzobacter terrae gen. nov., sp. nov. is proposed. The type strain of the type species is PSGM2-16T ( = KACC 17299T = DSM 27137T = NBRC 109598T).

scholarly journals Actinocatenispora thailandica gen. nov., sp. nov., a new member of the family Micromonosporaceae

2006 ◽  
Vol 56 (8) ◽  
pp. 1789-1794 ◽  
Author(s):  
Chitti Thawai ◽  
Somboon Tanasupawat ◽  
Takashi Itoh ◽  
Takuji Kudo
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Swamp Forest ◽  
The Family ◽  
Novel Genus And Species ◽  
Distinct Lineage ◽  
Genotypic Characteristics ◽  
The 16S Rrna Gene

Two actinomycete strains, TT2-10T and TT2-3, which produced long spore chains (more than 10 spores per chain), were isolated from peat swamp forest soil in Pattaloong Province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The chemotaxonomic characteristics of these strains coincided with those of the family Micromonosporaceae, i.e. cell-wall chemotype II, muramic acid of the N-glycolyl type, whole-cell sugar pattern D and type II phospholipids. Analysis of the 16S rRNA gene sequences also indicated that these strains constitute a distinct lineage within the family Micromonosporaceae, sharing 91.3–93.8 % sequence similarity with members of this family. On the basis of their phenotypic and genotypic characteristics and their phylogenetic position, these strains represent a novel genus and species, for which the name Actinocatenispora thailandica gen. nov., sp. nov. is proposed. The type strain of Actinocatenispora thailandica is strain TT2-10T (=JCM 12343T=PCU 235T=DSM 44816T).

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