scholarly journals Proposal of six species of moderately thermophilic, acidophilic, endospore-forming bacteria: Alicyclobacillus contaminans sp. nov., Alicyclobacillus fastidiosus sp. nov., Alicyclobacillus kakegawensis sp. nov., Alicyclobacillus macrosporangiidus sp. nov., Alicyclobacillus sacchari sp. nov. and Alicyclobacillus shizuokensis sp. nov.

2007 ◽  
Vol 57 (6) ◽  
pp. 1276-1285 ◽  
Author(s):  
Keiichi Goto ◽  
Kaoru Mochida ◽  
Yuko Kato ◽  
Mika Asahara ◽  
Rieko Fujita ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Saturated Fatty Acids ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Genetic Traits ◽  
Moderately Thermophilic

Moderately thermophilic, acidophilic, spore-forming bacteria (146 strains) were isolated from various beverages and environments. Based on the results of sequence analysis of the hypervariable region of the 16S rRNA gene, eight of the strains represent novel species of the genus Alicyclobacillus. These strains were designated 3-A191T, 4-A336T, 5-A83JT, 5-A167N, 5-A239-2O-AT, E-8, RB718T and S-TABT. Phylogenetic analyses of 16S rRNA and DNA gyrase B subunit (gyrB) nucleotide sequences confirmed that the eight strains belonged to the Alicyclobacillus clade. Cells of the eight strains were Gram-positive or Gram-variable, strictly aerobic and rod-shaped. The strains grew well under acidic and moderately thermal conditions, produced acid from various sugars, contained menaquinone 7 as the major isoprenoid quinone and did not produce guaiacol. ω-Alicyclic fatty acids were the predominant lipid component of strains 4-A336T, 5-A83JT, 5-A167N, RB718T and S-TABT. No ω-alicyclic fatty acids were detected in strains 3-A191T, 5-A239-2O-AT or E-8, but iso- and anteiso-branched fatty acids and small amounts of straight-chain saturated fatty acids were detected instead. According to the DNA–DNA hybridization data and distinct morphological, physiological, chemotaxonomical and genetic traits, the eight strains represent six novel species within the genus Alicyclobacillus, for which the following names are proposed: Alicyclobacillus contaminans sp. nov. (type strain 3-A191T=DSM 17975T=IAM 15224T), Alicyclobacillus fastidiosus sp. nov. (type strain S-TABT=DSM 17978T=IAM 15229T), Alicyclobacillus kakegawensis sp. nov. (type strain 5-A83JT=DSM 17979T=IAM 15227T), Alicyclobacillus macrosporangiidus sp. nov. (type strain 5-A239-2O-AT=DSM 17980T=IAM 15370T), Alicyclobacillus sacchari sp. nov. (type strain RB718T=DSM 17974T=IAM 15230T) and Alicyclobacillus shizuokensis sp. nov. (type strain 4-A336T=DSM 17981T=IAM 15226T).

scholarly journals Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov.

2007 ◽  
Vol 57 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Hana Yi ◽  
Peter Schumann ◽  
Jongsik Chun
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Novel Genus ◽  
Novel Genus And Species

An actinobacterial strain containing demethylmenaquinone DMK-9(H4) as the diagnostic isoprenoid quinone was isolated from a tidal flat sediment sample, from South Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain JC2054T represents a distinct phyletic line within the suborder Micrococcineae of the order Actinomycetales. The closest phylogenetic neighbour was Cellulomonas fermentans, with 94.7 % 16S rRNA gene sequence similarity. The novel isolate was strictly aerobic and slightly halophilic, with optimum growth occurring in 2–4 % (w/v) NaCl. Cells were non-motile, non-sporulating and rod-shaped. The peptidoglycan type was of the A-type of cross-linkage. l-ornithine was the diamino acid and d-glutamate represented the N-terminus of the interpeptide bridge. The predominant fatty acids were anteiso-branched and straight-chain fatty acids. The major polar lipids were phosphatidylinositol, diphosphatidylglycerol and an unknown phospholipid. The menaquinone composition of C. fermentans was determined to be MK-10(H4), MK-9(H4) and MK-8(H4) in the ratio 56 : 2 : 1. On the basis of the polyphasic evidence presented in this study, it is proposed that strain JC2054T should be classified as representing a novel genus and species of the suborder Micrococcineae, with the name Demequina aestuarii gen. nov., sp. nov. The type strain is JC2054T (=IMSNU 14027T=KCTC 9919T=JCM 12123T). In addition, it was clear from the phylogenetic analysis and chemotaxonomic data that C. fermentans does not belong to the genus Cellulomonas or any other recognized genera. Therefore, C. fermentans should be reclassified as representing a novel genus, for which the name Actinotalea fermentans gen. nov., comb. nov. is proposed, with strain DSM 3133T (=ATCC 43279T=CFBP 4259T=CIP 103003T=NBRC 15517T=JCM 9966T=LMG 16154T) as the type strain.

Mucilaginibacter soyangensis sp. nov., isolated from a lake

2014 ◽  
Vol 64 (Pt_2) ◽  
pp. 413-419 ◽  
Author(s):  
Yochan Joung ◽  
Haneul Kim ◽  
Heeyoung Kang ◽  
Beom-Il Lee ◽  
Tae-Seok Ahn ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type ◽  
Yellow Orange

A non-motile, yellow–orange-pigmented bacterial strain, designated HME6664T, was isolated from Lake Soyang, Republic of Korea. The major fatty acids of strain HME6664T were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c; 44.7 %) and iso-C15 : 0 (20.2 %). The DNA G+C content was 40.8 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6664T formed a lineage within the genus Mucilaginibacter . Strain HME6664T was closely related to Mucilaginibacter ximonensis (96.7 %), Mucilaginibacter dorajii (96.5 %) and Mucilaginibacter lappiensis (96.3 %). On the basis of the evidence presented in this study, strain HME6664T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter soyangensis sp. nov., is proposed. The type strain is HME6664T ( = KCTC 23261T = CECT 7824T).

scholarly journals Adhaeribacter aerophilus sp. nov., Adhaeribacter aerolatus sp. nov. and Segetibacter aerophilus sp. nov., isolated from air samples

2010 ◽  
Vol 60 (10) ◽  
pp. 2424-2429 ◽  
Author(s):  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
Jung-A Son ◽  
Soo-Jin Kim ◽  
Yi-Seul Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Novel Species ◽  
Fatty Acid Content ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Air Samples ◽  
Type Strains ◽  
Sequence Similarities

Three bacterial isolates from air samples in Korea, designated strains 6424S-25T, 6515J-31T and 6424S-61T, were characterized using a polyphasic approach. The cells were strictly aerobic, Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the phylum Bacteroidetes. Strains 6424S-25T and 6515J-31T showed 16S rRNA gene sequence similarities of 92.7–94.8 % to type strains of recognized species of the genus Adhaeribacter and strain 6424S-61T was closely related to Segetibacter koreensis Gsoil 664T (93.9 % similarity). The G+C contents of the DNA of strains 6424S-25T, 6515J-31T and 6424S-61T were 44.5, 43.9 and 38.4 mol%, respectively. Major fatty acids of strains 6424S-25T and 6515J-31T were summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B), iso-C15 : 0 and C16 : 1 ω5c. The fatty acid content of strain 6424S-61T mainly comprised iso-C15 : 1 G and iso-C15 : 0. Comparative analysis of phenotypic and phylogenetic traits indicated that strains 6424S-25T and 6515J-31T represented two novel species of the genus Adhaeribacter and that strain 6424S-61T should be considered as a novel species of the genus Segetibacter. The names Adhaeribacter aerophilus sp. nov. (type strain 6424S-25T =KACC 14118T =NBRC 106134T), Adhaeribacter aerolatus sp. nov. (type strain 6515J-31T =KACC 14117T =NBRC 106133T) and Segetibacter aerophilus sp. nov. (type strain 6424S-61T =KACC 14119T =NBRC 106135T) are proposed for these organisms.

scholarly journals Flavitalea populi gen. nov., sp. nov., isolated from soil of a Euphrates poplar (Populus euphratica) forest

2011 ◽  
Vol 61 (7) ◽  
pp. 1554-1560 ◽  
Author(s):  
Yang Wang ◽  
Feng Cai ◽  
Yali Tang ◽  
Jun Dai ◽  
Huan Qi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Populus Euphratica ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Euphrates Poplar ◽  
Taxonomic Approach

A novel strain, designated HY-50RT, isolated from soil of a Euphrates poplar (Populus euphratica) forest in Xinjiang, China, was characterized using a polyphasic taxonomic approach. Cells of the isolate were Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum Bacteroidetes, its closest relatives being Niastella populi THYL-44T (93.6 % similarity), Flavisolibacter ginsengisoli Gsoil 643T (93.5 %), Terrimonas ferruginea IAM 15098T (93.3 %) and Flavisolibacter ginsengiterrae Gsoil 492T (93.2 %). The major fatty acids were iso-C15 : 1 G (11.7 %), iso-C15 : 0 (19.6 %) and iso-C17 : 0 3-OH (19.3 %). The predominant menaquinone of strain HY-50RT was MK-7 and the genomic DNA G+C content was 46.8 mol%. Flexirubin-type pigments were not produced. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis, strain HY-50RT represents a novel species of a novel genus, for which the name Flavitalea populi gen. nov., sp. nov. is proposed. The type strain is HY-50RT ( = CCTCC AB 208255T  = NRRL B-59222T).

scholarly journals Hymenobacter yonginensis sp. nov., isolated from a mesotrophic artificial lake

2011 ◽  
Vol 61 (7) ◽  
pp. 1511-1514 ◽  
Author(s):  
Yochan Joung ◽  
Sung-Heun Cho ◽  
Haneul Kim ◽  
Seung Bum Kim ◽  
Kiseong Joh
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Artificial Lake ◽  
Type Strains

A non-motile, red-pigmented bacterium, designated strain HMD1010T, was isolated from an artificial lake located within the campus of Hankuk University of Foreign Studies, Yongin, Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD1010T formed a lineage within the genus Hymenobacter and was closely related to the type strains of Hymenobacter rigui (96.7 % sequence similarity) and H. gelipurpurascens (95.6 %). The major fatty acids were C16 : 1ω5c (21.9 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 17.2 %), iso-C15 : 0 (14.5 %) and summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I; 11.9 %). The DNA G+C content was 60.4 mol%. On the basis of the evidence presented in this study, strain HMD1010T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter yonginensis sp. nov. was proposed. The type strain is HMD1010T ( = KCTC 22745T  = CECT 7546T).

scholarly journals Nocardioides aestuarii sp. nov., isolated from tidal flat sediment

2004 ◽  
Vol 54 (6) ◽  
pp. 2151-2154 ◽  
Author(s):  
Hana Yi ◽  
Jongsik Chun
Keyword(s):  
Fatty Acids ◽  
Tidal Flat ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Cellular Fatty Acids ◽  
Gene Sequence Analysis

A strictly aerobic, non-motile, rod-shaped actinomycete, designated strain JC2056T, was isolated from a sediment sample of getbol, the tidal flat of Korea. Results of 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Nocardioides, with the highest similarity being to Nocardioides luteus KCTC 9575T (95·7 %). The major menaquinone was MK-8(H4), and predominant cellular fatty acids were iso-16 : 0 and iso-16 : 1 H. The DNA G+C content was 70 mol%. Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain JC2056T should be classified as a novel species, for which the name Nocardioides aestuarii sp. nov. is proposed; the type strain is JC2056T (=IMSNU 14029T=KCTC 9921T=JCM 12125T).

scholarly journals Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain

2004 ◽  
Vol 54 (5) ◽  
pp. 1793-1797 ◽  
Author(s):  
M. José Martínez-Cánovas ◽  
Victoria Béjar ◽  
Fernando Martínez-Checa ◽  
Rafael Páez ◽  
Emilia Quesada
Keyword(s):  
Fatty Acids ◽  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Carbon Sources ◽  
Tween 80 ◽  
Tween 20 ◽  
Extracellular Polysaccharides ◽  
Strictly Aerobic ◽  
Bacterial Strains

Two bacterial strains, F23T and R22T, have been isolated from hypersaline habitats in Málaga (S. Spain) and Murcia (E. Spain). The novel strains, similar to previously described Idiomarina species, are slightly curved rods, Gram-negative, chemo-organotrophic, strictly aerobic and motile by a single polar flagellum. Both strains produce catalase and oxidase. They hydrolyse aesculin, gelatin, casein, Tween 20, Tween 80 and DNA but not starch or tyrosine. The strains differ from the hitherto described Idiomarina species in their capacity to produce extracellular polysaccharides and their different patterns of carbon sources and antimicrobial susceptibility. They are moderate halophiles capable of growing in NaCl concentrations of 0·5 to 25 % w/v, the optimum being 3–5 % w/v. Cellular fatty acids are predominantly iso-branched. The main fatty acids in strain FP23T are 15 : 0 iso (26·75 %), 16 : 1ω7c (11·33 %) and 16 : 0 (11·73 %) whilst 15 : 0 iso (24·69 %), 17 : 0 iso (12·92 %) and 17 : 1ω9c (11·03 %) are predominant in strain R22T. The DNA G+C composition is 46·0 mol% in strain FP23T and 48·7 mol% in strain R22T. Phylogenetic analyses indicate conclusively that the two strains belong to the genus Idiomarina. DNA–DNA hybridization revealed that they represent novel species. In the light of the polyphasic evidence accumulated in this study, it is proposed that they be classified as novel species of the genus Idiomarina, with the names Idiomarina fontislapidosi sp. nov. (type strain F23T=CECT 5859T=LMG 22169T) and Idiomarina ramblicola sp. nov. (type strain R22T=CECT 5858T=LMG 22170T).

scholarly journals Description of Fabibacter halotolerans gen. nov., sp. nov. and Roseivirga spongicola sp. nov., and reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov.

2006 ◽  
Vol 56 (5) ◽  
pp. 1059-1065 ◽  
Author(s):  
Stanley C. K. Lau ◽  
Mandy M. Y. Tsoi ◽  
Xiancui Li ◽  
Ioulia Plakhotnikova ◽  
Sergey Dobretsov ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Bacterial Strains ◽  
Respiratory Quinone

Bacterial strains UST030701-097T and UST030701-084T were isolated from a marine sponge in the Bahamas. Both strains were pink-pigmented, Gram-negative, strictly aerobic and chemo-organotrophic. Cells of strain UST030701-097T were short, curved rods with fast-gliding motility, whereas those of strain UST030701-084T were straight rods with a less rapid gliding motion. The two strains had MK-7 as the major respiratory quinone and did not produce flexirubin-type pigments. The DNA G+C contents of strains UST030701-097T and UST030701-084T were 42.5 and 43.7 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the two strains belonged to the family ‘Flexibacteraceae’ of the phylum Bacteroidetes. 16S rRNA gene sequence similarity between strains UST030701-097T and UST030701-084T was 95.0 %; their closest relative was [Marinicola] seohaensis, with 93.3 % and 96.0 % sequence similarity, respectively. Phylogenetic tree topology indicated that the two strains belonged to the same lineage, but were on separate branches. Whilst strain UST030701-084T and [Marinicola] seohaensis were found on one branch, strain UST030701-097T was in another branch that had no species with validly published names. Based on the polyphasic taxonomic data obtained in the present study, we propose that strain UST030701-097T represents a novel genus and that strain UST030701-084T represents a novel species in the phylum Bacteroidetes. The genus Fabibacter gen. nov. is proposed, with strain UST030701-097T (=NRRL B-41220T=JCM 13334T) as the type strain of the type species, Fabibacter halotolerans sp. nov. Strain UST030701-084T (=NRRL B-41219T=JCM 13337T) is proposed as the type strain of Roseivirga spongicola sp. nov. In an earlier study, it was suggested that the genus Marinicola is a later heterotypic synonym of the genus Roseivirga. However, a formal proposal to reclassify [Marinicola] seohaensis, the only member of the genus Marinicola, has not yet been made. The results of phylogenetic analyses in this study support the reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov.

scholarly journals Mucilaginibacter flavus sp. nov., isolated from wetland

2014 ◽  
Vol 64 (Pt_4) ◽  
pp. 1304-1309 ◽  
Author(s):  
Yochan Joung ◽  
Haneul Kim ◽  
Beom-Il Lee ◽  
Heeyoung Kang ◽  
Tae-Su Kim ◽  
...  
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Content Type ◽  
Link Type

A non-motile, pale yellow, colony-forming strain, designated HME6839T, was isolated from the wetland of Jeju Island, Republic of Korea. The major fatty acids of strain HME6839T were summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), iso-C15 : 0 and C16 : 1ω5c. The DNA G+C content was 41.2 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HME6839T formed a lineage within the genus Mucilaginibacter . Strain HME6857T was closely related to Mucilaginibacter dorajii (96.7 %), Mucilaginibacter polysacchareus (96.5 %) and Mucilaginibacter lappiensis (96.3 %). On the basis of the chemotaxonomic and phylogenetic results presented in this study, strain HME6839T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter flavus sp. nov., is proposed. The type strain is HME6839T ( = KCTC 23441T = CECT 7857T).

scholarly journals Mesonia mobilis sp. nov., isolated from seawater, and emended description of the genus Mesonia

2006 ◽  
Vol 56 (10) ◽  
pp. 2433-2436 ◽  
Author(s):  
Olga I. Nedashkovskaya ◽  
Seung Bum Kim ◽  
Natalia V. Zhukova ◽  
Jangryul Kwak ◽  
Valery V. Mikhailov ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Rrna Gene Sequence ◽  
Gene Sequence Analysis ◽  
Emended Description

The taxonomic position of a novel marine, heterotrophic, strictly aerobic, gliding and yellow-pigmented bacterium, designated strain KMM 6059T, was determined. 16S rRNA gene sequence analysis revealed that this strain represents a member of the genus Mesonia. Phenotypic and chemotaxonomic data showed that the isolate represents a novel species of the genus Mesonia, for which the name Mesonia mobilis sp. nov. is proposed. The type strain is KMM 6059T (=KCTC 12708T=LMG 23670T). An emended description of the genus Mesonia based on the new data is also given.

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