scholarly journals Characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea

Microbiology ◽  
2010 ◽  
Vol 156 (1) ◽  
pp. 240-247 ◽  
Author(s):  
T. J. Evans ◽  
M. A. Crow ◽  
N. R. Williamson ◽  
W. Orme ◽  
N. R. Thomson ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Cancer Therapy ◽  
Secondary Metabolites ◽  
Host Range ◽  
High Efficiency ◽  
Fungal Infections ◽  
Broad Host Range ◽  
Potential Applications ◽  
Generalized Transduction

A phage (ΦOT8) isolated on Serratia sp. ATCC 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10−4 transductants per p.f.u.). ΦOT8 was shown to have a broad host range because it also infected a strain of Pantoea agglomerans isolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera. Serratia sp. ATCC 39006 and P. agglomerans produce a number of interesting secondary metabolites that have potential applications in cancer therapy and biocontrol of fungal infections. ΦOT8 has utility as a powerful functional genomics tool in these bacteria.

scholarly journals Genetic Characterization of Broad-Host-Range IncQ Plasmids Harboring blaVEB-18 in Vibrio Species

2017 ◽  
Vol 61 (7) ◽  
Author(s):  
Ruichao Li ◽  
Lianwei Ye ◽  
Zhiwei Zheng ◽  
Edward Wai Chi Chan ◽  
Sheng Chen
Keyword(s):  
Host Range ◽  
Genetic Characterization ◽  
Vibrio Species ◽  
Broad Host Range ◽  
Incq Plasmids

Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes

Gene ◽  
1995 ◽  
Vol 166 (1) ◽  
pp. 175-176 ◽  
Author(s):  
Michael E. Kovach ◽  
Philip H. Elzer ◽  
D. Steven Hill ◽  
Gregory T. Robertson ◽  
Michael A. Farris ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Host Range ◽  
Cloning Vector ◽  
Broad Host Range ◽  
Derivatives Of

scholarly journals Genomic and Proteomic Characterization of the Broad-Host-Range Salmonella Phage PVP-SE1: Creation of a New Phage Genus

2011 ◽  
Vol 85 (21) ◽  
pp. 11265-11273 ◽  
Author(s):  
S. B. Santos ◽  
A. M. Kropinski ◽  
P.-J. Ceyssens ◽  
H.- W. Ackermann ◽  
A. Villegas ◽  
...  
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Salmonella Phage

Characterization of Gram-positive broad host-range plasmids carrying a thermophilic replicon

1991 ◽  
Vol 142 (4) ◽  
pp. 389-396 ◽  
Author(s):  
E De Rossi ◽  
P Brigidi ◽  
M Rossi ◽  
D Matteuzzi ◽  
G Riccardi
Keyword(s):  
Host Range ◽  
Broad Host Range ◽  
Gram Positive ◽  
Broad Host Range Plasmids

scholarly journals JD419, a Staphylococcus aureus Phage With a Unique Morphology and Broad Host Range

2021 ◽  
Vol 12 ◽  
Author(s):  
Tingting Feng ◽  
Sebastian Leptihn ◽  
Ke Dong ◽  
Belinda Loh ◽  
Yan Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Host Range ◽  
Antibiotic Resistance Genes ◽  
Multidrug Resistant ◽  
Complete Characterization ◽  
Resistant Bacteria ◽  
Broad Host Range ◽  
Gene Engineering ◽  
Mrsa Strains

Phage therapy represents a possible treatment option to cure infections caused by multidrug-resistant bacteria, including methicillin and vancomycin-resistant Staphylococcus aureus, to which most antibiotics have become ineffective. In the present study, we report the isolation and complete characterization of a novel phage named JD219 exhibiting a broad host range able to infect 61 of 138 clinical strains of S. aureus tested, which included MRSA strains as well. The phage JD419 exhibits a unique morphology with an elongated capsid and a flexible tail. To evaluate the potential of JD419 to be used as a therapeutic phage, we tested the ability of the phage particles to remain infectious after treatment exceeding physiological pH or temperature. The activity was retained at pH values of 6.0–8.0 and below 50°C. As phages can contain virulence genes, JD419’s complete genome was sequenced. The 45509 bp genome is predicted to contain 65 ORFs, none of which show homology to any known virulence or antibiotic resistance genes. Genome analysis indicates that JD419 is a temperate phage, despite observing rapid replication and lysis of host strains. Following the recent advances in synthetic biology, JD419 can be modified by gene engineering to remove prophage-related genes, preventing potential lysogeny, in order to be deployed as a therapeutic phage.

scholarly journals Isolation and characterization of novel broad host range bacteriophages of Vibrio cholerae O1 from Bengal

2018 ◽  
Vol 10 (2) ◽  
pp. 84
Author(s):  
BanwarilalL Sarkar ◽  
Sounak Sarkar ◽  
Mayukh Das ◽  
TusharSuvra Bhowmick ◽  
Hemanta Koley ◽  
...  
Keyword(s):  
Host Range ◽  
Vibrio Cholerae ◽  
Broad Host Range ◽  
Isolation And Characterization ◽  
Vibrio Cholerae O1

scholarly journals A detailed in silico analysis of secondary metabolite biosynthesis clusters in the genome of the broad host range plant pathogenic fungus Sclerotinia sclerotiorum

2019 ◽  
Author(s):  
Carolyn Graham-Taylor ◽  
Lars G Kamphuis ◽  
Mark Derbyshire
Keyword(s):  
Secondary Metabolites ◽  
Host Range ◽  
Secondary Metabolite ◽  
Sclerotinia Sclerotiorum ◽  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Gene Clusters ◽  
Sequence Diversity ◽  
Broad Host Range ◽  
Data Set

Abstract Background The broad host range pathogen Sclerotinia sclerotiorum infects over 400 plant species and causes substantial yield losses in crops worldwide. Secondary metabolites are known to play important roles in the virulence of plant pathogens, but little is known about the secondary metabolite repertoire of S. sclerotiorum. In this study, we predicted secondary metabolite biosynthetic gene clusters in the genome of S. sclerotiorum and analysed their expression during infection of Brassica napus using an existing transcriptome data set. We also investigated their sequence diversity among a panel of 25 previously published S. sclerotiorum isolate genomes.Results We identified 80 putative secondary metabolite clusters. Over half of the clusters contained at least three transcriptionally coregulated genes. Comparative genomics revealed clusters homologous to clusters in the closely related plant pathogen Botrytis cinerea for production of carotenoids, hydroxamate siderophores, DHN melanin and botcinic acid. We also identified putative phytotoxin clusters that can potentially produce the polyketide sclerin and an epipolythiodioxopiperazine. Secondary metabolite clusters were enriched in subtelomeric genomic regions, and those containing paralogues showed a particularly strong association with repeats. The positional bias we identified was borne out by intraspecific comparisons that revealed putative secondary metabolite genes suffered more presence / absence polymorphisms and exhibited a significantly higher sequence diversity than other genes.Conclusions These data suggest that S. sclerotiorum produces numerous secondary metabolites during plant infection and that their gene clusters undergo enhanced rates of mutation, duplication and recombination in subtelomeric regions. The microevolutionary regimes leading to S. sclerotiorum secondary metabolite diversity have yet to be elucidated. Several potential phytotoxins documented in this study provide the basis for future functional analyses.

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