scholarly journals The Effects of Addition of Mononucleotides on Sma nuc Endonuclease Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Julia Romanova ◽  
Maria Filimonova
Keyword(s):  
Enzyme Activity ◽  
Serratia Marcescens ◽  
Competitive Inhibitor ◽  
Endonuclease Activity ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Carbohydrate Residue ◽  
Partial Type ◽  
Hydrolysis Of ◽  
Hydrolysis Of Dna

Examination of the effects of mononucleotides on Sma nuc endonuclease originated from Gram negative bacteriumSerratia marcescensdisplayed that any mononucleotide produced by Sma nuc during hydrolysis of DNA or RNA may regulate the enzyme activity affecting the RNase activity without pronounced influence on the activity towards DNA. The type of carbohydrate residue in mononucleotides does not affect the regulation. In contrast, the effects depend on the type of bases in nucleotides. AMP or dAMP was classified as a competitive inhibitor of partial type. GMP, UMP, and CMP were found to be uncompetitive inhibitors that suggest a specific site(s) for the nucleotide(s) binding in Sma nuc endonuclease.

scholarly journals Draft genome sequence of a prodigiosin-hyperproducing Serratia marcescens strain isolated from Cairo, Egypt

2021 ◽  
Author(s):  
Nora M Elkenawy ◽  
Noha H Youssef ◽  
Ramy K Aziz ◽  
Magdy A Amin ◽  
Aymen S Yassin
Keyword(s):  
Serratia Marcescens ◽  
Genome Sequence ◽  
Draft Genome ◽  
Industrial Applications ◽  
Draft Genome Sequence ◽  
Infection Frequency ◽  
Negative Bacterium ◽  
Gram Negative

Abstract Serratia marcescens is a Gram-negative bacterium with both environmental and host-associated strains. Pigmentation is reportedly inversely correlated with infection frequency, and prodigiosin is one of Serratia pigments that has medical and industrial applications. Here, we report the draft genome sequence of prodigiosin-hyperproducing Serratia marcescens strain N2, isolated from Cairo, Egypt. The sequence is assembled into 142 contigs, with a combined size of 5,570,793 bp. The assembled genome carries typical S. marcescens genes, with potential prodigiosin-biosynthesizing genes detected.

scholarly journals Complete Genome Sequence of Serratia Phage Muldoon

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Soren Campbell ◽  
Cameron Atkison ◽  
Russell Moreland ◽  
Mei Liu ◽  
Jolene Ramsey ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Opportunistic Infections ◽  
Trna Genes ◽  
Negative Bacterium ◽  
Protein Coding ◽  
Gram Negative ◽  
Content Type ◽  
Protein Coding Genes

Serratia marcescens is a ubiquitous Gram-negative bacterium that is linked with emerging opportunistic infections. In this report, we describe the isolation and annotation of an S. marcescens myophage called Muldoon. Related to T4-like phages, such as Serratia phage PS2, Muldoon contains 257 predicted protein-coding genes and 4 tRNA genes.

Diagnostic Enzymology of a-L-Iduronidase with Special Reference to a Sulphated Disaccharide Derived from Heparin

1982 ◽  
Vol 62 (2) ◽  
pp. 193-201 ◽  
Author(s):  
J. J. Hopwood ◽  
Vivienne Muller
Keyword(s):  
Enzyme Activity ◽  
Competitive Inhibitor ◽  
Ph Profile ◽  
Ph Values ◽  
Substrate Structure ◽  
Cultured Skin ◽  
Bivalent Cations ◽  
Competitive Inhibitors ◽  
Specific Ion Effect ◽  
Hydrolysis Of

1. Iduronosyl anhydro[1-3H]mannitol 6-sulphate (IMs), iduronosyl anhydro[1-3H]mannitol, phenyl iduronide (PhI) and 4-methylumbelliferyl iduronide have been compared as substrates for the diagnostic estimation of α-l-iduronidase activity present in human leucocyte and cultured skin fibroblast homogenates. The pH profile of leucocyte and fibroblast iduronidase activity was dependent on substrate structure and concentration, the ionic strength and the nature of the buffer ion used in the assay mixture. 2. NaCl, KBr and Na2SO4 were shown to be parabolic competitive inhibitors of IMs activity, the K1 with fibroblast homogenates being 34, 13.4 and 0.22 mmol/l respectively. NaCl and KBr were shown to have a primary salt effect on the interaction between enzyme and substrate but Na2SO4 appeared to have a specific ion effect at a cationic binding site. 3. NaCl inhibited the hydrolysis of IMs at all pH values studied, whereas NaCl concentrations of 0.2 mol/l inhibited the hydrolysis of PhI at pH values below 3.8 but activated the enzyme at higher incubation pH values. 4. Cu2+ was shown to be a potent non-competitive inhibitor of IMs enzyme activity with an apparent Kl, of approximately 0.02 mmol/l. The enzyme activity was inhibited by Fe2+ (Kl 4 mmol/l), Hg2+ and Ag+, but has not significantly been affected by other univalent or bivalent cations. 5. The presence of solvent and salt effects on apparent Km but not the Vmax. suggest that the binding of IMs to the enzyme involved charge neutralization, and it is inferred that two cationic binding sites are present at the active site. It is postulated that one site specifically binds to the iduronic acid carboxyl group, the other to the 6-sulphate of the anhydromannitol moiety.

HORMONE AND ENZYME ASSAYS IN PREGNANCY

1975 ◽  
Vol 78 (2) ◽  
pp. 364-372
Author(s):  
Arne Christensen ◽  
P. E. Hagelid
Keyword(s):  
Enzyme Activity ◽  
Linear Part ◽  
Competitive Inhibitor ◽  
Plasma Samples ◽  
Simple Method ◽  
Normal Enzyme ◽  
Lag Period ◽  
Enzyme Activity Pattern ◽  
Hydrolysis Of

ABSTRACT A rapid and simple method for determination of the placental cystineaminopeptidase (P-CAP) activity in plasma is presented. The enzymecatalysed hydrolysis of the substrate, l-cystine-bis-p-nitroanilide, is followed in a spectrophotometer by reading the absorbance of the product p-nitroaniline at 380 nm. The absorbance increases linearily with time after a lag-period of 30 seconds to 5 min. The reaction is followed for about 10 min. and the increase in absorbtion per min is calculated from the linear part of the absorbtion curve. The test could also be performed by taking 2 readings at about 5 min intervals. In the studies of the enzyme kinetics a competitive inhibitor of the reaction was found in plasma. The amount of the inhibitory substances seemed to be nearly constant in the plasma samples studied from normal pregnant women as well as in plasma samples with high enzyme activity (twin-pregnancy) and low enzyme activity (severe pre-eclampsia). The normal enzyme activity pattern showed an increase from about 210 days of pregnancy and towards the term. A coefficient of correlation of + 0.83 with the more time consuming method using l-cystine-di-βnaphtylamide as substrate was found. It was concluded that the present method could replace the method described by Babuna & Yenen (1966) at least from about 210 days of pregnancy and where a rapid answer is necessary.

scholarly journals Draft Genome Sequences of 38 Serratia marcescens Isolates Associated with Acroporid Serratiosis

2019 ◽  
Vol 8 (14) ◽  
Author(s):  
Nicole C. Elledge ◽  
Ron I. Eytan ◽  
Lee J. Pinnell ◽  
Reavelyn Pray ◽  
Jessica L. Joyner ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Draft Genome ◽  
Negative Bacterium ◽  
Genome Sequences ◽  
Gram Negative ◽  
Content Type

Serratia marcescens is a Gram-negative bacterium causally linked to acroporid serratiosis, a form of white pox disease implicated in the decline of elkhorn corals. We report draft genomes of 38 S. marcescens isolates collected from host and nonhost sources.

scholarly journals A Possible Outbreak by Serratia Marcescens: Genetic Relatedness between Clinical and Environmental Strains

2021 ◽  
Vol 18 (18) ◽  
pp. 9814
Author(s):  
Giuseppina Caggiano ◽  
Francesco Triggiano ◽  
Giusy Diella ◽  
Francesca Apollonio ◽  
Marco Lopuzzo ◽  
...  
Keyword(s):  
Intensive Care ◽  
Serratia Marcescens ◽  
Critically Ill Patients ◽  
Nosocomial Infections ◽  
Genetic Relatedness ◽  
University Hospital ◽  
Genetic Profile ◽  
Negative Bacterium ◽  
Ample Evidence ◽  
Gram Negative

Serratia marcescens (SM) is a Gram-negative bacterium that is frequently found in the environment. Since 1913, when its pathogenicity was first demonstrated, the number of infections caused by SM has increased. There is ample evidence that SM causes nosocomial infections in immunocompromised or critically ill patients admitted to the intensive care units (ICUs), but also in newborns admitted to neonatal ICUs (NICUs). In this study, we evaluated the possible genetic correlation by PFGE between clinical and environmental SM strains from NICU and ICU and compared the genetic profile of clinical strains with strains isolated from patients admitted to other wards of the same hospital. We found distinct clonally related groups of SM strains circulating among different wards of a large university hospital. In particular, the clonal relationship between clinical and environmental strains in NICU and ICU 1 was highlighted. The identification of clonal relationships between clinical and environmental strains in the wards allowed identification of the epidemic and rapid implementation of adequate measures to stop the spread of SM.

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