scholarly journals Animal Bones Char Solubilization by Gel-EntrappedYarrowia lipolyticaon Glycerol-Based Media

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Maria Vassileva ◽  
Bettina Eichler-Lobermann ◽  
Antonia Reyes ◽  
Nikolay Vassilev
Keyword(s):  
Citric Acid ◽  
Immobilized Cells ◽  
Yeast Cells ◽  
Maximum Efficiency ◽  
Batch Mode ◽  
Animal Bone ◽  
Animal Bones ◽  
Repeated Batch Fermentation ◽  
Repeated Batch ◽  
Single Batch

Citric acid was produced with free and k-carrageenan-entrapped cells of the yeastYarrowia lipolyticain single and repeated batch-shake-flask fermentations on glycerol-based media. Simultaneous solubilization of hydroxyapatite of animal bone origin (HABO) was tested in all experiments. The highest citric acid production by free yeast cells of 20.4 g/L and 18.7 g/L was reached after 96 h of fermentation in the absence and presence of 3 g/L HABO, respectively. The maximum values for the same parameter achieved by gel-entrapped cells in conditions of single batch and repeated-batch fermentation processes were 18.7 g/L and 28.1 g/L registered after 96 h and the 3d batch cycle, respectively. The highest citric acid productivity of 0.58 g L−1h−1was obtained with immobilized cells in repeated batch mode of fermentation when the added hydroxyapatite of 3 g/L was solubilized to 399 mg/L whereas the maximum efficiency of 89.0% was obtained with 1 g/L of HABO.

scholarly journals Effect of Washing Yeast Cells on Alcohol Productivity in Repeated Batch Fermentation in Beet Molasses

1988 ◽  
Vol 83 (7) ◽  
pp. 501-503
Author(s):  
Kazuhito MORIYA ◽  
Haruyuki IEFUJI ◽  
Hitoshi SHIMOI ◽  
Shun-ichi SATO ◽  
Makoto TADENUMA
Keyword(s):  
Batch Fermentation ◽  
Yeast Cells ◽  
Beet Molasses ◽  
Repeated Batch Fermentation ◽  
Repeated Batch

Evaluation of Immobilization Conditions for Enhanced Production of Gentamicin in Repeated Batch Operations by Micromonospora Echinospora

2007 ◽  
Vol 5 (1) ◽  
Author(s):  
Himabindu Meenavilli ◽  
Ravichandra Potumarthi ◽  
Annapurna Jetty
Keyword(s):  
Immobilized Cells ◽  
Batch Processes ◽  
Curing Time ◽  
Batch Mode ◽  
Enhanced Production ◽  
Alginate Concentration ◽  
Micromonospora Echinospora ◽  
Bead Diameter ◽  
Repeated Batch ◽  
Immobilized Beads

Gentamicin production by immobilized cells of M. echinospora has been studied in repeated-batch processes using different immobilizing carriers such as calcium-alginate, carrageenan, agar, PVA, celite, polyurethane foam and activated carbon. With ca-alginate, gentamicin production was maximum (1096 mg/l) and the bead stability was good, whereby alginate was selected for further experimentation. The role of alginate concentration, CaCl2 concentration, bead diameter, curing time and bead inoculum quantity were studied. Better gentamicin outputs (1549 mg/l) were attained with alginate 4% (w/v), CaCl2 0.2 M, bead diameter 2.69 mm, curing time 18 h and inoculum quantity 20% v/v for six repeated cycles in shake flask. Productivity of gentamicin was increased to 1.8 fold in a fluidized bed reactor with optimized alginate immobilized beads and increased reusability. The FBR was operated for 30 days in fed batch mode operation.

scholarly journals A lag-time model for substrate and product diffusion through hydroxyethylcellulose gels used for immobilization of yeast cells

2011 ◽  
Vol 30 (1) ◽  
pp. 85
Author(s):  
Elena Velickova ◽  
Slobodanka Kuzmanova ◽  
Eleonora Winkelhausen
Keyword(s):  
Diffusion Coefficient ◽  
Batch Fermentation ◽  
Lag Time ◽  
Yeast Cells ◽  
Product Diffusion ◽  
Time Model ◽  
A Value ◽  
Repeated Batch Fermentation ◽  
Repeated Batch ◽  
Diffusion Properties

Diffusion models were tested to select the most appropriate model for measuring the diffusion of glucose and ethanol trough hydroxyethylcellulose (HEC) cryogels. The lag-time model was selected as accurate, rapid and reliable for evaluating the diffusivity of the solutes. The diffusion coefficient of glucose through the HEC cryogel loaded with cells was estimated to be 6.9×10-6 cm2/s, while the diffusion coefficient of ethanol had a value of 2.5×10-5 cm2/s. The cells inside the gel matrix were not a barrier for the transport of the solute molecules. The diffusion properties of the cryogel were also examined in a real system. The parameters characterizing the repeated batch fermentation of glucose to ethanol by entrapped Saccharomyces cerevisiae cells gave evidence of very good diffusion properties of the HEC gel.

scholarly journals Repeated batch fermentation for protease production using biofilm fermentation of Bacillus sp.

2018 ◽  
Vol 6 (2) ◽  
pp. 29-38
Author(s):  
Ripa Moni ◽  
Mohammed Salahuddin ◽  
Md Abdullah Al Noman Khan ◽  
Umme Salma Zohora ◽  
Mohammad Shahedur Rahman
Keyword(s):  
Batch Fermentation ◽  
Immobilized Cells ◽  
Protease Production ◽  
Similar Amount ◽  
Environment Friendly ◽  
Complete Replacement ◽  
Protease Enzyme ◽  
Repeated Batch Fermentation ◽  
Repeated Batch ◽  
Medium Supplementation

Repeated batch fermentation is a stable, economically viable and environment friendly fermentation system. Biofilm of Bacillus subtilis was investigated for repeated batch fermentation for the production of protease enzyme. It was found that complete replacement of spent medium with fresh medium supplementation in biofilm produced similar amount of protease that is about 225 U/ml of enzyme in successive batches. Moreover, unlike some solid carriers the EPS layer does not entrap much enzyme inside it. Therefore, instead of using immobilized cells, biofilm of B. subtilis can be a good candidate for protease enzyme production using repeated batch fermentation.Jahangirnagar University J. Biol. Sci. 6(2): 29-38, 2017 (December)

scholarly journals Repeated-Batch Fermentation of Cheese Whey for Semi-Continuous Lactic Acid Production Using Mixed Cultures at Uncontrolled pH

2019 ◽  
Vol 11 (12) ◽  
pp. 3330 ◽  
Author(s):  
Vincenzo Luongo ◽  
Grazia Policastro ◽  
Anish Ghimire ◽  
Francesco Pirozzi ◽  
Massimiliano Fabbricino
Keyword(s):  
Lactic Acid ◽  
Retention Time ◽  
Cheese Whey ◽  
Hydraulic Retention Time ◽  
Lactic Acid Production ◽  
Oxygen Demand ◽  
Batch Mode ◽  
Repeated Batch Fermentation ◽  
Repeated Batch ◽  
Ph Conditions

The paper investigates mixed-culture lactate (LA) fermentation of cheese whey (CW) in order to verify the possibility of using waste materials as feedstock to produce a product with high economic potential. The fermentation performance of two reactors operating in repeated-batch mode under uncontrolled pH conditions and various hydraulic retention time and feeding conditions was evaluated in terms of LA production. Five experimental phases were conducted. The hydraulic retention time (HRT) was varied from 1 to 4 days to verify its effect on the process performance. The best results, corresponding to the maximum LA concentration (20.1 g LA/L) and the maximum LA yield (0.37 g chemical oxygen demand (COD)(LA)/g COD(CW)), were reached by feeding the reactors with cheese whey alone and setting the HRT to 2 days. The maximum productivity of lactic acid (10.6 g LA/L/day) was observed when the HRT was decreased to 1 day.

A view from the Greek side

2016 ◽  
Vol 7 (1) ◽  
pp. 35-50
Author(s):  
Gunnel Ekroth
Keyword(s):  
Comparative Perspective ◽  
Ancient Greek ◽  
Greek Religion ◽  
Bone Material ◽  
Archaeological Material ◽  
Animal Bone ◽  
Animal Bones ◽  
Starting Point ◽  
Integrated Study ◽  
Written Sources

This paper addresses the animal bone material from ancient Qumran, from the comparative perspective of zooarchaeological evidence recovered in ancient Greek cult contexts. The article offers an overview of the paramount importance of animal bones for the understanding of ancient Greek religion and sacrificial practices in particular, followed by a review of the Qumran material, taking as its starting point the zooarchaeological evidence and the archaeological find contexts. The methodological complications of letting the written sources guide the interpretation of the archaeological material are explored, and it is suggested that the Qumran bones are to be interpreted as remains of ritual meals following animal sacrifices, as proposed by Jodi Magness. The presence of calcined bones additionally supports the proposal that there was once an altar in area L130, and it is argued that the absence of preserved altar installations in many ancient sanctuaries cannot be used as an argument against their ever having been present. Finally, the similarities between Israelite and Greek sacrificial practices are touched upon, arguing for the advantages of a continued and integrated study of these two sacrificial systems based on the zooarchaeological evidence.

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