scholarly journals Lipid Mediator Informatics and Proteomics in Inflammation-Resolution

2006 ◽  
Vol 6 ◽  
pp. 589-614 ◽  
Author(s):  
Yan Lu ◽  
Song Hong ◽  
Katherine Gotlinger ◽  
Charles Serhan
Keyword(s):  
Mass Spectrometry ◽  
Peptide Mapping ◽  
Lipid Mediators ◽  
Automated System ◽  
Gas Chromatography Mass Spectrometry ◽  
Lipid Mediator ◽  
Enzyme Linked Immunosorbent Assay ◽  
Accurate Analysis ◽  
Spatial Production ◽  
Experimental Inflammation

Lipid mediator informatics is an emerging area denoted to the identification of bioactive lipid mediators (LMs) and their biosynthetic profiles and pathways. LM informatics and proteomics applied to inflammation, systems tissues research provides a powerful means of uncovering key biomarkers for novel processes in health and disease. By incorporating them with system biology analysis, we review here our initial steps toward elucidating relationships among a range of bimolecular classes and provide an appreciation of their roles and activities in the pathophysiology of disease. LM informatics employing liquid chromatography-ultraviolet-tandem mass spectrometry (LC-UV-MS/MS), gas chromatography-mass spectrometry (GC-MS), computer-based automated systems equipped with databases and novel searching algorithms, and enzyme-linked immunosorbent assay (ELISA) to evaluate and profile temporal and spatial production of mediators combined with proteomics at defined points during experimental inflammation and its resolution enable us to identify novel mediators in resolution. The automated system including databases and searching algorithms is crucial for prompt and accurate analysis of these lipid mediators biosynthesized from precursor polyunsaturated fatty acids such as eicosanoids, resolvins, and neuroprotectins, which play key roles in human physiology and many prevalent diseases, especially those related to inflammation. This review presents detailed protocols used in our lab for LM informatics and proteomics using LC-UV-MS/MS, GC-MS, ELISA, novel databases and searching algorithms, and 2-dimensional gel electrophoresis and LC-nanospray-MS/MS peptide mapping.

Comparative Assessment of Fumonisin in Grain-Based Foods by ELISA, GC-MS, and HPLC

1994 ◽  
Vol 57 (2) ◽  
pp. 169-172 ◽  
Author(s):  
JAMES J. PESTKA ◽  
JUAN I. AZCONA-OLIVERA ◽  
RONALD D. PLATTNER ◽  
FIORENZA MINERVINI ◽  
M. BRUNO DOKO ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Food Samples ◽  
Comparative Assessment ◽  
The Other ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cereal Samples ◽  
Related Compounds ◽  
Positive Results

Seventy-one (71) food samples were analyzed for the mycotoxin fumonisin by a monoclonal antibody based competitive enzyme-linked immunosorbent assay (ELISA). Fumonisins were detected primarily in corn-based products with 7/12, 2/2 and 1/3 and 1/7 yellow cornmeal, blue cornmeal, corn muffin mix, and mixed grain cereal samples yielding positive results, respectively. When the positive samples and randomly selected negative samples were assessed by other methods, correlations (r values) between ELISA and gas chromatography-mass spectrometry (GC-MS), ELISA and high-pressure liquid chromatography (HPLC) and GC-MS and HPLC were 0.478 (p < 0.05), 0.512 (p < 0.05), and 0.946 (p < 0.01), respectively. The results suggested that although the immunoassay could be used for screening of fumonisin in food samples, higher estimates were attained by ELISA than by the other two methods particularly in the more contaminated samples. These observations may result from differences in sample preparation among the methods or because of the presence of structurally related compounds in extracts that are detectable by ELISA but not the other two methods.

Gold Nanoparticles-Amplified Indirect Competitive Enzyme-Linked Immunosorbent Assay of BDE-121 Using a Monoclonal Antibody

2021 ◽  
Vol 21 (10) ◽  
pp. 5036-5043
Author(s):  
Yan Qiao ◽  
Qing-Yun Cai
Keyword(s):  
Mass Spectrometry ◽  
Monoclonal Antibody ◽  
Gold Nanoparticles ◽  
Immunosorbent Assay ◽  
Inhibitory Concentration ◽  
High Sensitivity ◽  
High Specificity ◽  
Gas Chromatography Mass Spectrometry ◽  
Enzyme Linked Immunosorbent Assay ◽  
Good Recovery

In this study, we developed a monoclonal antibody against 2,3’,4,5’,6-pentabromodiphenylether (BDE-121) using a synthesized hapten, and established an indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), using gold nanoparticles, to amplify the signal. The monoclonal antibody showed high specificity, with a half inhibitory concentration (IC50) value of 2.78 ng/mL, towards BDE-121. The developed IC-ELISA exhibited high sensitivity and stability as well as good recovery. The intra-assay deviation is below 6.8% and the inter-assay deviations range from 6.5% to 8.7%. The assay of the actual samples was found to be consistent with those of gas chromatography/mass spectrometry (GC/MS).

Migration Detection of Residual Solvents from Composite Packaging Film to Food by GC-Ms

2011 ◽  
Vol 380 ◽  
pp. 218-221
Author(s):  
Wen Li Dong ◽  
Yun Jing Li ◽  
Wan Feng Zhang
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
High Sensitivity ◽  
Gas Chromatography Mass Spectrometry ◽  
Packaging Film ◽  
Accurate Analysis ◽  
Residual Solvent ◽  
Residual Solvents ◽  
Food Medium

An analytical method was developed to measure the migration of residual solvent from composite packaging film to food medium. By using gas chromatography / mass spectrometry, migration levels of toluene and ethyl acetate, the main residual solvents in composite packaging film were measured in food so that the migration characteristic of toluene and ethyl acetate in different simulations was studied. The results show that all the linear correlation coefficient were greater than 0.999 and the detection limit was lower than 0.005μg/ml. This method is of high sensitivity, accurate analysis of quantity and can meet the practical demands.

scholarly journals UHPLC-HRMS and GC-MS Screening of a Selection of Synthetic Cannabinoids and Metabolites in Urine of Consumers

Medicina ◽  
2020 ◽  
Vol 56 (8) ◽  
pp. 408 ◽  
Author(s):  
Manuela Pellegrini ◽  
Emilia Marchei ◽  
Esther Papaseit ◽  
Magí Farré ◽  
Simona Zaami
Keyword(s):  
Mass Spectrometry ◽  
Formic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Synthetic Cannabinoids ◽  
Screening Method ◽  
Ammonium Formate ◽  
Gas Chromatography Mass Spectrometry ◽  
Accurate Analysis

Background and Objectives: The use of synthetic cannabinoids has increased around the world. As a result, the implementation of accurate analysis in human biological matrices is relevant and fundamental. Two different analytical technologies, ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) and high-sensitivity gas chromatography-mass spectrometry (GC-MS) were used for the determination of three synthetic cannabinoids JWH-122, JWH 210, UR-144 and their metabolites in urine of consumers. Materials and Methods: Sample preparation included an initial hydrolysis with β-glucuronidase and liquid-liquid extraction. The UHPLC-HRMS method included a Kinetex 2.6 u Biphenyl 100A (100 × 2.1 mm, 2.6 μm) (Phenomenex, Italy) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode was used (mass range 100–1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m × 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40–550 m/z). Results: Both methods have been successfully used for screening of parent synthetic cannabinoids and their metabolites in urine samples of consumers. Conclusions: The screening method applied JWH-122, JWH-210, UR-144 and their metabolites in urine of consumers can be applied to other compounds of the JWH family.

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