scholarly journals Limb Regeneration inXenopus laevisFroglet

2006 ◽  
Vol 6 ◽  
pp. 26-37 ◽  
Author(s):  
Makoto Suzuki ◽  
Nayuta Yakushiji ◽  
Yasuaki Nakada ◽  
Akira Satoh ◽  
Hiroyuki Ide ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Limb Regeneration ◽  
Anuran Amphibian ◽  
Organ Regeneration ◽  
Undifferentiated Cells ◽  
Representative Process

Limb regeneration in amphibians is a representative process of epimorphosis. This type of organ regeneration, in which a mass of undifferentiated cells referred to as the “blastema” proliferate to restore the lost part of the amputated organ, is distinct from morphallaxis as observed, for instance, in Hydra, in which rearrangement of pre-existing cells and tissues mainly contribute to regeneration. In contrast to complete limb regeneration in urodele amphibians, limb regeneration inXenopus, an anuran amphibian, is restricted. In this review of some aspects regarding adult limb regeneration inXenopus laevis, we suggest that limb regeneration in adultXenopus, which is pattern/tissue deficient, also represents epimorphosis.

scholarly journals Hyperinnervation improves Xenopus laevis limb regeneration

2018 ◽  
Vol 433 (2) ◽  
pp. 276-286 ◽  
Author(s):  
Kazumasa Mitogawa ◽  
Aki Makanae ◽  
Akira Satoh
Keyword(s):  
Xenopus Laevis ◽  
Limb Regeneration

scholarly journals Nerve-Independence of Limb Regeneration in Larval Xenopus laevis Is Correlated to the Level of fgf-2 mRNA Expression in Limb Tissues

2001 ◽  
Vol 231 (2) ◽  
pp. 436-446 ◽  
Author(s):  
S.M. Cannata ◽  
C. Bagni ◽  
S. Bernardini ◽  
B. Christen ◽  
S. Filoni
Keyword(s):  
Xenopus Laevis ◽  
Mrna Expression ◽  
Limb Regeneration

Effect of retinoic acid on limb regeneration in Xenopus laevis

1983 ◽  
Vol 61 (12) ◽  
pp. 2698-2702 ◽  
Author(s):  
Steven R. Scadding
Keyword(s):  
Retinoic Acid ◽  
Xenopus Laevis ◽  
Limb Regeneration ◽  
Dependent Manner ◽  
African Clawed Frog ◽  
Long Term Effect ◽  
Aquarium Water ◽  
Dose Dependent ◽  
Clawed Frog

The objective of this experiment was to determine the effect of retinoic acid on the process of limb regeneration in the African clawed frog, Xenopus laevis. Limbs were bilaterally amputated through the radio-ulna and then treated for 15 days with retinoic acid in the aquarium water, at 3, 15, or 75 IU/mL. The retinoic acid inhibited limb regeneration in a dose-dependent manner, reduced the length of the regenerates, and produced irregularities in the morphogenesis of the cartilage rod in the regenerate. The regenerated limbs were removed after 150 days by amputation through the humerus, and the limbs were again allowed to regenerate. In the retinoic acid treated animals, despite the fact that retinoic acid treatment had been discontinued over 4 months previously, limb regeneration was still inhibited. These results suggest that retinoic acid has a long-term effect on the treated animals.

scholarly journals Limb Regeneration in Xenopus laevis Froglet

2006 ◽  
Vol 1 ◽  
pp. 26-37
Author(s):  
Makoto Suzuki ◽  
Nayuta Yakushiji ◽  
Yasuaki Nakada ◽  
Akira Satoh ◽  
Hiroyuki Ide ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Limb Regeneration

scholarly journals Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs

Development ◽  
2021 ◽  
Author(s):  
Can Aztekin ◽  
Tom W. Hiscock ◽  
John Gurdon ◽  
Jerome Jullien ◽  
John Marioni ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Single Cell ◽  
Progenitor Cells ◽  
Limb Development ◽  
Ex Vivo ◽  
Limb Regeneration ◽  
Cell Formation ◽  
Regeneration Potential ◽  
Wound Epidermis ◽  
Extracellular Environment

Absence of a specialized wound epidermis is hypothesized to block limb regeneration in higher vertebrates. However, the factors preventing its formation in regeneration-incompetent animals are poorly understood. To characterize the endogenous molecular and cellular regulators of specialized wound epidermis formation in Xenopus laevis tadpoles, and the loss of their regeneration-competency during development, we used single-cell transcriptomics and ex vivo regenerating limb cultures. Transcriptomic analysis revealed that the specialized wound epidermis is not a novel cell state, but a re-deployment of the apical-ectodermal-ridge (AER) program underlying limb development. Enrichment of secreted inhibitory factors, including Noggin, a morphogen expressed in developing cartilage/bone progenitor cells, are identified as key inhibitors of AER cell formation in regeneration-incompetent tadpoles. These factors can be overridden by Fgf10, which operates upstream of Noggin and blocks chondrogenesis. These results indicate that manipulation of the extracellular environment and/or chondrogenesis may provide a strategy to restore regeneration potential in higher vertebrates.

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