scholarly journals Genetic characterization of African swine fever virus in Romania during 2018-2019 outbreak

2019 ◽  
Author(s):  
Vlad Petrovan ◽  
Mihai Turcitu ◽  
Lilia Matei ◽  
Vlad Constantinescu ◽  
Mihaela Zaulet
Keyword(s):  
Molecular Characterization ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Viral Disease ◽  
Republic Of Georgia ◽  
The Republic ◽  
Socio Economic Impact ◽  
Genotype Ii

AbstractAfrican swine fever (ASF) is a highly contagious and lethal viral disease of swine with significant socio-economic impact in the developed and developing world. Since its reintroduction in 2007 in the Republic of Georgia, the disease has spread dramatically thorough Europe and Asia. Among the most affected countries in Europe is Romania, which initially reported the disease in 2017 and in 2018-2019 lost about 1 million pigs. There is no molecular characterization of the virus circulating in Romania during that reported period; therefore, the purpose of this study was to provide an initial molecular characterization using samples collected from two farms affected by ASFV. The causative strain belongs to genotype II, and its closest relatives are the strains circulating in Belgium, Russia, and China.

scholarly journals Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30

2021 ◽  
Vol 20 (03) ◽  
pp. 18-25
Author(s):  
Duy T. Do
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
Complex Structure ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Amino Acid Sequences ◽  
Sequencing Analysis ◽  
Genes Encoding ◽  
Genotype Ii

African swine fever (ASF) is reported as a highly contagious hemorrhage lethal disease of domestic and wild swine. The causative agent of ASF is a large enveloped DNA virus with a complex structure. There are twenty-four ASFV genotypes described to date. However, in Vietnam, only genotypes II had been previously described. The genetic characterization of ASFV enhances the understanding of ASF epidemiology in terms of the extent, severity, source, and potential genetic variations among ASFV strains circulating in Southern Vietnam. Twenty ASFV strains were collected from pigs with ASFV-infected clinical signs from 10 provinces during 2019 - 2020. Partial B646L (p72) gene, complete E183L (p54), and CP204L (p30) genes were amplified, purified, and sequenced. Phylogenetic analysis confirmed the circulation of genotype II by both the partial B646L (p72) and full-length E183 (p54) gene sequencing. Analysis of the p72, p54, and p30 regions did not indicate any change in the nucleotide and amino acid sequences among these strains. The results of this study revealed that these ASFVs shared high homology to ASFV isolates detected in Northern Vietnam and China.

Genetic characterization of African swine fever virus in Cameroon, 2010–2018

2019 ◽  
Vol 57 (4) ◽  
pp. 316-324 ◽  
Author(s):  
Abel Wade ◽  
Jenna Elizabeth Achenbach ◽  
Carmina Gallardo ◽  
Tirumala Bharani K. Settypalli ◽  
Abdoulkadiri Souley ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

scholarly journals Molecular characterization of African swine fever virus in apparently healthy domestic pigs in Uganda

2014 ◽  
Vol 13 (25) ◽  
pp. 2491-2499 ◽  
Author(s):  
Kalenzi Atuhaire David ◽  
Ochwo Sylvester ◽  
Afayoa Mathias ◽  
Mwesigwa Savannah ◽  
Norbert Mwiine Frank ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs ◽  
Apparently Healthy

scholarly journals Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1237
Author(s):  
Hyun-Joo Kim ◽  
Ki-Hyun Cho ◽  
Ji-Hyoung Ryu ◽  
Min-Kyung Jang ◽  
Ha-Gyeong Chae ◽  
...  
Keyword(s):  
South Korea ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Genetic Characteristics ◽  
Tandem Repeat Sequences ◽  
Central Variable Region ◽  
Pig Farm

On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

scholarly journals Genetic profile of African swine fever virus responsible for the 2019 outbreak in northern Malawi

2020 ◽  
Author(s):  
J.N. Hakizimana ◽  
G. Kamwendo ◽  
J.L.C. Chulu ◽  
O. Kamana ◽  
H.J. Nauwynck ◽  
...  
Keyword(s):  
Food Security ◽  
Genome Sequencing ◽  
African Swine Fever Virus ◽  
Phylogenetic Reconstruction ◽  
African Swine Fever ◽  
Genetic Profile ◽  
African Countries ◽  
Partial Genome ◽  
Genotype Ii

Abstract Background African swine fever (ASF) is an infectious transboundary animal disease which causes high mortality, approaching 100% in domestic pigs and it is currently considered as the most serious constraint to domestic pig industry and food security globally. Despite regular ASF outbreaks within Malawi, few studies have genetically characterized the causative ASF virus (ASFV). This study aimed at genetic characterization of ASFV responsible for the 2019 outbreak in northern Malawi. The disease confirmation was done by polymerase chain reaction (PCR) followed by molecular characterization of the causative ASFV by partial genome sequencing and phylogenetic reconstruction of the B646L (p72) gene, nucleotide alignment of the intergenic region (IGR) between I73R and I329L genes and translation of the central variable region (CVR) coded by B602L gene. Results All thirteen samples collected during this study in Karonga district in September 2019 were ASFV-positive and after partial genome sequencing and phylogenetic reconstruction of the B646L (p72) gene, the viruses clustered into ASFV p72 genotype II. The viruses characterized in this study lacked a GAATATATAG fragment between the I173R and the I329L genes and were classified as IGR I variants. Furthermore, the tetrameric amino acid repeats within the CVR of the B602L gene of the 2019 Malawian ASFV reported in this study had the signature BNDBNDBNAA, 100% similar to ASFV responsible for the 2013 and 2017 ASF outbreaks in Zambia and Tanzania, respectively. Conclusions The results of this study confirm an ASF outbreak in Karonga district in northern Malawi in September 2019. The virus was closely related to other p72 genotype II ASFV that caused outbreaks in neighboring eastern and southern African countries, emphasizing the possible regional transboundary transmission of this ASFV genotype. These findings call for a concerted regional and international effort to control the spread of ASF in order to improve nutritional and food security.

scholarly journals Molecular Characterization of African Swine Fever Virus, China, 2018

2018 ◽  
Vol 24 (11) ◽  
pp. 2131-2133 ◽  
Author(s):  
Shengqiang Ge ◽  
Jinming Li ◽  
Xiaoxu Fan ◽  
Fuxiao Liu ◽  
Lin Li ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

Long amplicon sequencing for improved genetic characterization of African swine fever virus

2020 ◽  
Vol 285 ◽  
pp. 113946
Author(s):  
David A. Meekins ◽  
Jessie D. Trujillo ◽  
Natasha N. Gaudreault ◽  
Igor Morozov ◽  
Daniel Pérez-Núñez ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Amplicon Sequencing ◽  
Fever Virus
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