scholarly journals Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30

2021 ◽  
Vol 20 (03) ◽  
pp. 18-25
Author(s):  
Duy T. Do
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
Complex Structure ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Amino Acid Sequences ◽  
Sequencing Analysis ◽  
Genes Encoding ◽  
Genotype Ii

African swine fever (ASF) is reported as a highly contagious hemorrhage lethal disease of domestic and wild swine. The causative agent of ASF is a large enveloped DNA virus with a complex structure. There are twenty-four ASFV genotypes described to date. However, in Vietnam, only genotypes II had been previously described. The genetic characterization of ASFV enhances the understanding of ASF epidemiology in terms of the extent, severity, source, and potential genetic variations among ASFV strains circulating in Southern Vietnam. Twenty ASFV strains were collected from pigs with ASFV-infected clinical signs from 10 provinces during 2019 - 2020. Partial B646L (p72) gene, complete E183L (p54), and CP204L (p30) genes were amplified, purified, and sequenced. Phylogenetic analysis confirmed the circulation of genotype II by both the partial B646L (p72) and full-length E183 (p54) gene sequencing. Analysis of the p72, p54, and p30 regions did not indicate any change in the nucleotide and amino acid sequences among these strains. The results of this study revealed that these ASFVs shared high homology to ASFV isolates detected in Northern Vietnam and China.

scholarly journals Genetic characterization of African swine fever virus in Romania during 2018-2019 outbreak

2019 ◽  
Author(s):  
Vlad Petrovan ◽  
Mihai Turcitu ◽  
Lilia Matei ◽  
Vlad Constantinescu ◽  
Mihaela Zaulet
Keyword(s):  
Molecular Characterization ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Viral Disease ◽  
Republic Of Georgia ◽  
The Republic ◽  
Socio Economic Impact ◽  
Genotype Ii

AbstractAfrican swine fever (ASF) is a highly contagious and lethal viral disease of swine with significant socio-economic impact in the developed and developing world. Since its reintroduction in 2007 in the Republic of Georgia, the disease has spread dramatically thorough Europe and Asia. Among the most affected countries in Europe is Romania, which initially reported the disease in 2017 and in 2018-2019 lost about 1 million pigs. There is no molecular characterization of the virus circulating in Romania during that reported period; therefore, the purpose of this study was to provide an initial molecular characterization using samples collected from two farms affected by ASFV. The causative strain belongs to genotype II, and its closest relatives are the strains circulating in Belgium, Russia, and China.

scholarly journals Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1474
Author(s):  
Elisabeth Lopez ◽  
Juanita van Heerden ◽  
Laia Bosch-Camós ◽  
Francesc Accensi ◽  
Maria Jesus Navas ◽  
...  
Keyword(s):  
Vaccine Development ◽  
African Swine Fever Virus ◽  
Virulent Strain ◽  
Sequence Similarity ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Cross Protection ◽  
Sub Saharan Africa ◽  
Genotype I ◽  
Genotype Ii

African swine fever (ASF) has become the major threat for the global swine industry. Furthermore, the epidemiological situation of African swine fever virus (ASFV) in some endemic regions of Sub-Saharan Africa is worse than ever, with multiple virus strains and genotypes currently circulating in a given area. Despite the recent advances on ASF vaccine development, there are no commercial vaccines yet, and most of the promising vaccine prototypes available today have been specifically designed to fight the genotype II strains currently circulating in Europe, Asia, and Oceania. Previous results from our laboratory have demonstrated the ability of BA71∆CD2, a recombinant LAV lacking CD2v, to confer protection against homologous (BA71) and heterologous genotype I (E75) and genotype II (Georgia2007/01) ASFV strains, both belonging to same clade (clade C). Here, we extend these results using BA71∆CD2 as a tool trying to understand ASFV cross-protection, using phylogenetically distant ASFV strains. We first observed that five out of six (83.3%) of the pigs immunized once with 106 PFU of BA71∆CD2 survived the tick-bite challenge using Ornithodoros sp. soft ticks naturally infected with RSA/11/2017 strain (genotype XIX, clade D). Second, only two out of six (33.3%) survived the challenge with Ken06.Bus (genotype IX, clade A), which is phylogenetically more distant to BA71∆CD2 than the RSA/11/2017 strain. On the other hand, homologous prime-boosting with BA71∆CD2 only improved the survival rate to 50% after Ken06.Bus challenge, all suffering mild ASF-compatible clinical signs, while 100% of the pigs immunized with BA71∆CD2 and boosted with the parental BA71 virulent strain survived the lethal challenge with Ken06.Bus, without almost no clinical signs of the disease. Our results confirm that cross-protection is a multifactorial phenomenon that not only depends on sequence similarity. We believe that understanding this complex phenomenon will be useful for designing future vaccines for ASF-endemic areas.

Genetic characterization of African swine fever virus in Cameroon, 2010–2018

2019 ◽  
Vol 57 (4) ◽  
pp. 316-324 ◽  
Author(s):  
Abel Wade ◽  
Jenna Elizabeth Achenbach ◽  
Carmina Gallardo ◽  
Tirumala Bharani K. Settypalli ◽  
Abdoulkadiri Souley ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

scholarly journals Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1237
Author(s):  
Hyun-Joo Kim ◽  
Ki-Hyun Cho ◽  
Ji-Hyoung Ryu ◽  
Min-Kyung Jang ◽  
Ha-Gyeong Chae ◽  
...  
Keyword(s):  
South Korea ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Genetic Characteristics ◽  
Tandem Repeat Sequences ◽  
Central Variable Region ◽  
Pig Farm

On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

scholarly journals Genetic profile of African swine fever virus responsible for the 2019 outbreak in northern Malawi

2020 ◽  
Author(s):  
J.N. Hakizimana ◽  
G. Kamwendo ◽  
J.L.C. Chulu ◽  
O. Kamana ◽  
H.J. Nauwynck ◽  
...  
Keyword(s):  
Food Security ◽  
Genome Sequencing ◽  
African Swine Fever Virus ◽  
Phylogenetic Reconstruction ◽  
African Swine Fever ◽  
Genetic Profile ◽  
African Countries ◽  
Partial Genome ◽  
Genotype Ii

Abstract Background African swine fever (ASF) is an infectious transboundary animal disease which causes high mortality, approaching 100% in domestic pigs and it is currently considered as the most serious constraint to domestic pig industry and food security globally. Despite regular ASF outbreaks within Malawi, few studies have genetically characterized the causative ASF virus (ASFV). This study aimed at genetic characterization of ASFV responsible for the 2019 outbreak in northern Malawi. The disease confirmation was done by polymerase chain reaction (PCR) followed by molecular characterization of the causative ASFV by partial genome sequencing and phylogenetic reconstruction of the B646L (p72) gene, nucleotide alignment of the intergenic region (IGR) between I73R and I329L genes and translation of the central variable region (CVR) coded by B602L gene. Results All thirteen samples collected during this study in Karonga district in September 2019 were ASFV-positive and after partial genome sequencing and phylogenetic reconstruction of the B646L (p72) gene, the viruses clustered into ASFV p72 genotype II. The viruses characterized in this study lacked a GAATATATAG fragment between the I173R and the I329L genes and were classified as IGR I variants. Furthermore, the tetrameric amino acid repeats within the CVR of the B602L gene of the 2019 Malawian ASFV reported in this study had the signature BNDBNDBNAA, 100% similar to ASFV responsible for the 2013 and 2017 ASF outbreaks in Zambia and Tanzania, respectively. Conclusions The results of this study confirm an ASF outbreak in Karonga district in northern Malawi in September 2019. The virus was closely related to other p72 genotype II ASFV that caused outbreaks in neighboring eastern and southern African countries, emphasizing the possible regional transboundary transmission of this ASFV genotype. These findings call for a concerted regional and international effort to control the spread of ASF in order to improve nutritional and food security.

Long amplicon sequencing for improved genetic characterization of African swine fever virus

2020 ◽  
Vol 285 ◽  
pp. 113946
Author(s):  
David A. Meekins ◽  
Jessie D. Trujillo ◽  
Natasha N. Gaudreault ◽  
Igor Morozov ◽  
Daniel Pérez-Núñez ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Amplicon Sequencing ◽  
Fever Virus

Genetic characterization of African swine fever virus from domestic pigs in India

2021 ◽  
Author(s):  
Katherukamem Rajukumar ◽  
Dhanapal Senthilkumar ◽  
Govindarajulu Venkatesh ◽  
Fateh Singh ◽  
Vishnu P. Patil ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals Molecular Characterization of African Swine Fever Virus Isolates in Estonia in 2014–2019

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 582 ◽  
Author(s):  
Annika Vilem ◽  
Imbi Nurmoja ◽  
Tarmo Niine ◽  
Taavi Riit ◽  
Raquel Nieto ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Limited Area ◽  
First Case ◽  
Central Variable Region ◽  
Genotype Ii

After the extensive spread of the African swine fever virus (ASFV) genotype II in Eastern Europe, the first case of African swine fever (ASF) in Estonia was diagnosed in September 2014. By the end of 2019, 3971 ASFV-positive wild boars were found, and 27 domestic pig outbreaks were reported. A selection of ASFV isolates from wild boar and domestic pigs (during the period of September 2014–2019) was molecularly characterized using standardized genotyping procedures. One of the proven markers to characterize this virus is the central variable region (CVR) within the B602L gene. In summer 2015, a new ASFV genotype II CVR variant 2 (GII-CVR2) was confirmed in Estonia. The results suggest that the GII-CVR2 variant was only confirmed in wild boar from a limited area in southern Estonia in 2015 and 2016. In addition to GII-CVR2, a single nucleotide polymorphism (SNP) that resulted in amino acid change was identified within the genotype II CVR variant 1 (GII-CVR1). The GII-CVR1/SNP1 strain was isolated in Estonia in November 2016. Additional GII-CVR1/SNP1 cases were confirmed in two neighbouring counties, as well as in one outbreak farm in June 2017. Based on the available data, no GII-CVR2 and GII-CVR1/SNP1 have been reported by other affected European countries. The spread of variant strains in Estonia has been limited over time, and restricted to a relatively small area.

Sign in / Sign up

Export Citation Format

Share Document