scholarly journals Isolation and Genetic Characterization of African Swine Fever Virus from Domestic Pig Farms in South Korea, 2019

Viruses ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1237
Author(s):  
Hyun-Joo Kim ◽  
Ki-Hyun Cho ◽  
Ji-Hyoung Ryu ◽  
Min-Kyung Jang ◽  
Ha-Gyeong Chae ◽  
...  
Keyword(s):  
South Korea ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Genetic Characteristics ◽  
Tandem Repeat Sequences ◽  
Central Variable Region ◽  
Pig Farm

On 17 September 2019, the first outbreak of African swine fever in a pig farm was confirmed in South Korea. By 9 October, 14 outbreaks of ASF in domestic pigs had been diagnosed in 4 cities/counties. We isolated viruses from all infected farms and performed genetic characterization. The phylogenetic analysis showed that all of fourteen ASFV isolates in South Korea belong to genotype II and serogroup 8. Additionally, all isolates had an intergenic region (IGR) II variant with additional tandem repeat sequences (TRSs) between the I73R and I329L genes and showed characteristics of central variable region (CVR) 1 of the B602L gene and IGR 1 of MGF 505 9R/10R genes. These are identical to the genetic characteristics of some European isolates and Chinese isolates.

scholarly journals Molecular Characterization of African Swine Fever Virus Isolates in Estonia in 2014–2019

Pathogens ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 582 ◽  
Author(s):  
Annika Vilem ◽  
Imbi Nurmoja ◽  
Tarmo Niine ◽  
Taavi Riit ◽  
Raquel Nieto ◽  
...  
Keyword(s):  
Wild Boar ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Limited Area ◽  
First Case ◽  
Central Variable Region ◽  
Genotype Ii

After the extensive spread of the African swine fever virus (ASFV) genotype II in Eastern Europe, the first case of African swine fever (ASF) in Estonia was diagnosed in September 2014. By the end of 2019, 3971 ASFV-positive wild boars were found, and 27 domestic pig outbreaks were reported. A selection of ASFV isolates from wild boar and domestic pigs (during the period of September 2014–2019) was molecularly characterized using standardized genotyping procedures. One of the proven markers to characterize this virus is the central variable region (CVR) within the B602L gene. In summer 2015, a new ASFV genotype II CVR variant 2 (GII-CVR2) was confirmed in Estonia. The results suggest that the GII-CVR2 variant was only confirmed in wild boar from a limited area in southern Estonia in 2015 and 2016. In addition to GII-CVR2, a single nucleotide polymorphism (SNP) that resulted in amino acid change was identified within the genotype II CVR variant 1 (GII-CVR1). The GII-CVR1/SNP1 strain was isolated in Estonia in November 2016. Additional GII-CVR1/SNP1 cases were confirmed in two neighbouring counties, as well as in one outbreak farm in June 2017. Based on the available data, no GII-CVR2 and GII-CVR1/SNP1 have been reported by other affected European countries. The spread of variant strains in Estonia has been limited over time, and restricted to a relatively small area.

scholarly journals Genetic characterisation of African swine fever virus from 2017 outbreaks in Zambia: Identification of p72 genotype II variants in domestic pigs

2018 ◽  
Vol 85 (1) ◽  
Author(s):  
Edgar Simulundu ◽  
Yona Sinkala ◽  
Herman M. Chambaro ◽  
Andrew Chinyemba ◽  
Frank Banda ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Economic Losses ◽  
African Region ◽  
Swine Industry ◽  
African Countries ◽  
Central Variable Region ◽  
Close Relationship ◽  
Haemorrhagic Disease

African swine fever (ASF) is a contagious haemorrhagic disease associated with causing heavy economic losses to the swine industry in many African countries. In 2017, Zambia experienced ASF outbreaks in Mbala District (Northern province) and for the first time in Isoka and Chinsali districts (Muchinga province). Meanwhile, another outbreak was observed in Chipata District (Eastern province). Genetic analysis of part of the B646L gene, E183L gene, CP204L gene and the central variable region of the B602L gene of ASF virus (ASFV) associated with the outbreaks in Mbala and Chipata districts was conducted. The results revealed that the ASFV detected in Mbala District was highly similar to that of the Georgia 2007/1 isolate across all the genome regions analysed. In contrast, while showing close relationship with the Georgia 2007/1 virus in the B646L gene, the ASFV detected in Chipata District showed remarkable genetic variation in the rest of the genes analysed. These results suggest that the Georgia 2007/1-like virus could be more diverse than what was previously thought, underscoring the need of continued surveillance and monitoring of ASFVs within the south-eastern African region to better understand their epidemiology and the relationships between outbreaks and their possible origin.

Genetic characterization of African swine fever virus in Cameroon, 2010–2018

2019 ◽  
Vol 57 (4) ◽  
pp. 316-324 ◽  
Author(s):  
Abel Wade ◽  
Jenna Elizabeth Achenbach ◽  
Carmina Gallardo ◽  
Tirumala Bharani K. Settypalli ◽  
Abdoulkadiri Souley ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus

scholarly journals Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir

2005 ◽  
Vol 86 (11) ◽  
pp. 3153-3162 ◽  
Author(s):  
F. Bernardi ◽  
S. A. Nadin-Davis ◽  
A. I. Wandeler ◽  
J. Armstrong ◽  
A. A. B. Gomes ◽  
...  
Keyword(s):  
Animal Species ◽  
Genetic Characterization ◽  
Variable Region ◽  
Epidemiological Studies ◽  
Genetic Classification ◽  
Livestock Species ◽  
Central Variable Region ◽  
Vampire Bats

Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.

scholarly journals Amino Acid Tandem Repeats within a Late Viral Gene Define the Central Variable Region of African Swine Fever Virus

Virology ◽  
1996 ◽  
Vol 220 (1) ◽  
pp. 20-27 ◽  
Author(s):  
P.M. IRUSTA ◽  
M.V. BORCA ◽  
G.F. KUTISH ◽  
Z. LU ◽  
E. CALER ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tandem Repeats ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Variable Region ◽  
Fever Virus ◽  
Viral Gene ◽  
Central Variable Region

Long amplicon sequencing for improved genetic characterization of African swine fever virus

2020 ◽  
Vol 285 ◽  
pp. 113946
Author(s):  
David A. Meekins ◽  
Jessie D. Trujillo ◽  
Natasha N. Gaudreault ◽  
Igor Morozov ◽  
Daniel Pérez-Núñez ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Amplicon Sequencing ◽  
Fever Virus

Genetic characterization of African swine fever virus from domestic pigs in India

2021 ◽  
Author(s):  
Katherukamem Rajukumar ◽  
Dhanapal Senthilkumar ◽  
Govindarajulu Venkatesh ◽  
Fateh Singh ◽  
Vishnu P. Patil ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Fever Virus ◽  
Domestic Pigs

scholarly journals Genetic characterization of African swine fever virus in Romania during 2018-2019 outbreak

2019 ◽  
Author(s):  
Vlad Petrovan ◽  
Mihai Turcitu ◽  
Lilia Matei ◽  
Vlad Constantinescu ◽  
Mihaela Zaulet
Keyword(s):  
Molecular Characterization ◽  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Viral Disease ◽  
Republic Of Georgia ◽  
The Republic ◽  
Socio Economic Impact ◽  
Genotype Ii

AbstractAfrican swine fever (ASF) is a highly contagious and lethal viral disease of swine with significant socio-economic impact in the developed and developing world. Since its reintroduction in 2007 in the Republic of Georgia, the disease has spread dramatically thorough Europe and Asia. Among the most affected countries in Europe is Romania, which initially reported the disease in 2017 and in 2018-2019 lost about 1 million pigs. There is no molecular characterization of the virus circulating in Romania during that reported period; therefore, the purpose of this study was to provide an initial molecular characterization using samples collected from two farms affected by ASFV. The causative strain belongs to genotype II, and its closest relatives are the strains circulating in Belgium, Russia, and China.

scholarly journals Genetic analysis of African swine fever virus based on major genes encoding p72, p54 and p30

2021 ◽  
Vol 20 (03) ◽  
pp. 18-25
Author(s):  
Duy T. Do
Keyword(s):  
Genetic Characterization ◽  
African Swine Fever Virus ◽  
Complex Structure ◽  
Clinical Signs ◽  
African Swine Fever ◽  
Amino Acid Sequences ◽  
Sequencing Analysis ◽  
Genes Encoding ◽  
Genotype Ii

African swine fever (ASF) is reported as a highly contagious hemorrhage lethal disease of domestic and wild swine. The causative agent of ASF is a large enveloped DNA virus with a complex structure. There are twenty-four ASFV genotypes described to date. However, in Vietnam, only genotypes II had been previously described. The genetic characterization of ASFV enhances the understanding of ASF epidemiology in terms of the extent, severity, source, and potential genetic variations among ASFV strains circulating in Southern Vietnam. Twenty ASFV strains were collected from pigs with ASFV-infected clinical signs from 10 provinces during 2019 - 2020. Partial B646L (p72) gene, complete E183L (p54), and CP204L (p30) genes were amplified, purified, and sequenced. Phylogenetic analysis confirmed the circulation of genotype II by both the partial B646L (p72) and full-length E183 (p54) gene sequencing. Analysis of the p72, p54, and p30 regions did not indicate any change in the nucleotide and amino acid sequences among these strains. The results of this study revealed that these ASFVs shared high homology to ASFV isolates detected in Northern Vietnam and China.

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