scholarly journals In silico and In vitro evaluation of the anti-inflammatory and antioxidant potential of Cymbopogon citratus from North-western Himalayas

2020 ◽  
Author(s):  
Deeksha Salaria ◽  
Rajan Rolta ◽  
Nitin Sharma ◽  
Kamal Dev ◽  
Anuradha Sourirajan ◽  
...  
Keyword(s):  
In Silico ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Perennial Herb ◽  
Binding Potential ◽  
Western Himalayas ◽  
Cymbopogon Citratus ◽  
Anti Inflammatory

AbstractCymbopogon citratus which is an aromatic perennial herb belonging to family Gramineae is known for its application in food and healthcare industry. The present study attempts to evaluate the potential of essential oil from Cymbopogon citratus (CEO) as an anti-inflammatory and antioxidant agent. CEO showed significant DPPH radical scavenging activity (IC50 - 91.0 ± 9.25 µg/ml), as compared to Ascorbic acid (IC50-33.38 ± 2.29 µg/ml). CEO also exhibited significant in-vitro anti-inflammatory activity with IC50 - 397.11± 1.45µg/ml) as compared to diclofenac sodium (IC50 - 682.98 ± 7.47 µg/ml). Chemical constituents of the oil was determined using Gas Chromatography/Mass Spectroscopy, showed that 8-methyl-3,7-Nonadien-2-one (E), α-Pinene, limonene, citral, limonene oxide and Epoxy-α-terpenyl acetate were the major constituents. The in silico molecular docking study showed phytocompounds of CEO (Caryophyllene oxide and β-caryophyllene) have considerable binding potential with 1HD2 and 5IKQ receptors. PASS prediction of these phytocompounds also confirmed strong anti-inflammatory activity of C. citratus. The ADMET analysis also showed that these phytocompounds are safer to replace the synthetic drugs with side effects. This work establishes the anti inflammatory potential of CEO as an alternative to existing therapeutic approach to treatment of inflammation and also natural source of antioxidant compounds.

scholarly journals Bioassay Directed Isolation and Biological Evaluation of Compounds Isolated fromRubus fairholmianusGard.

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Blassan Plackal George ◽  
Parimelazhagan Thangaraj ◽  
Cheruthazhakkatt Sulaiman ◽  
Shanmughavel Piramanayagam ◽  
Sathish Kumar Ramaswamy
Keyword(s):  
Benzoic Acid ◽  
In Silico ◽  
Inflammatory Diseases ◽  
Radical Scavenging ◽  
Biological Evaluation ◽  
Acetone Extract ◽  
Inflammatory Activity ◽  
Target Proteins ◽  
Acid Compound

Thein vitroandin silicoanalysis ofRubus fairholmianusacetone extract for antioxidant, antiproliferative, and anti-inflammatory activity led to the isolation of six compounds. Amongst all the six isolated compounds tested, 1-(2-hydroxyphenyl)-4-methylpentan-1-one (compound1) and 2-[(3-methylbutoxy) carbonyl] benzoic acid (compound2) were found to be more active in inhibiting BRCA and COX target proteins, which also showed the better results for DPPH and ABTS radical scavenging assays. The promising results of this investigation emphasize the importance of usingR. fairholmianusin the treatment of radical generated disorders mainly cancer and other inflammatory diseases.

scholarly journals IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ALCHORNEA CORDIFOLIA (SCHUMACH AND THONN.) MULL. ARG. AND ANTROCARYON KLAINEANUM PIERRE EXTRACTS

2020 ◽  
pp. 129-135
Author(s):  
ROSETTE CHRISTELLE NDJIB ◽  
STEVE VALDI DJOVA ◽  
CHRISTELLE WAYOUE KOM ◽  
AGBOR GABRIEL AGBOR ◽  
AMINA MAMAT ◽  
...  
Keyword(s):  
Concentration Level ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Inflammatory Activity ◽  
Phytochemical Screening ◽  
Reducing Activity ◽  
Anti Inflammatory ◽  
Β Carotene

Objective: This study aims to evaluate the in vitro antioxidant, anti-inflammatory activities of the aqueous and hydroethanolic extracts recipe of Alchornea cordifolia and Antrocaryon klaineanum. A preliminary phytochemical screening was carried out. Methods: The total phenols content was determined by the Folin Ciocalteu reagent method, while the antioxidant activity of both extracts was characterized by the 2-2diphenyl-1-picrilhidrazil (DPPH) and β-carotene assays. The anti-inflammatory activity of the extracts was evaluated as the inhibition of Bovine Serum Albumin (BSA) denaturation and antiproteinase activity. Results: The aqueous extracts of Alchornea cordifolia and Antrocaryon klaineanum contained more polyphenols [270 mg Ascorbic acid equivalent (AAE)/g dry weight (dw)] than the hydroethanolic recipe extract (262.41 mg AAE/g dw) at the same concentration level. On the other hand, the aqueous and hydroethanolic recipe extract had the same radical scavenging activity with the antiradical power of 0.851 and 0.830, respectively. Similarly, the recipe extract had the same reducing activity with reducing the power of 94.2±2.03 mg EAA/g dw and 97.4±4.16 mg EAA/g dw for the aqueous and hydroethanolic recipe extract of Alchornea cordifolia and Antrocaryon klaineanum respectively. For the anti-inflammatory activity it was observed that both extracts possess the same activity as Diclofenac® with an IC50 of 50.21 μg/ml. The phytochemical screening of the extracts revealed the presence of alkaloids, flavonoids, carbohydrates, phenols and tannins, which may account for their activities. Conclusion: The plant recipe extract studied possess antioxidant and anti-inflammatory potentials, which may be beneficiary to its consumers.

scholarly journals Cascade Transformations of 1-R-Ethynyl-9,10-anthraquinones with Amidines: Expanding Access to Isoaporphinoid Alkaloids

Molecules ◽  
2021 ◽  
Vol 26 (22) ◽  
pp. 6883
Author(s):  
Sergey Francevich Vasilevsky ◽  
Ol’ga Leonidovna Krivenko ◽  
Irina Vasilievna Sorokina ◽  
Dmitry Sergeevich Baev ◽  
Tatyana Genrikhovna Tolstikova ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Concanavalin A ◽  
In Silico ◽  
Intraperitoneal Injection ◽  
Antitumor Drug ◽  
Inflammatory Activity ◽  
Antitumor Properties ◽  
Anti Inflammatory

The interaction of acetamidine and phenylamidine with peri-R-ethynyl-9,10-anthraquinones in refluxing n-butanol leads to the formation of cascade transformations products: addition/elimination/cyclization―2-R-7H-dibenzo[de,h]quinolin-7-ones and(or) 2-R-3-aroyl-7H-dibenzo[de,h]quinolin-7-ones. The anti-inflammatory and antitumor properties of the new 2-R-7H-dibenzo[de,h]quinolin-7-ones were investigated in vivo, in vitro, and in silico. The synthesized compounds exhibit high anti-inflammatory activity at dose 20 mg/kg (intraperitoneal injection) in the models of exudative (histamine-induced) and immunogenic (concanavalin A-induced) inflammation. Molecular docking data demonstrate that quinolinones can potentially intercalate into DNA similarly to the antitumor drug doxorubicin.

scholarly journals In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

2020 ◽  
Vol 12 (2) ◽  
pp. 222-232
Author(s):  
Turgut TASKIN ◽  
Murat DOGAN ◽  
Muhammet E. CAM ◽  
Talip SAHIN ◽  
Ismail SENKARDES
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Direct Methanol ◽  
Anti Inflammatory

Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.

scholarly journals Chemical Constituents from the Roots and Rhizomes of Asarum heterotropoides var. mandshuricum and the In Vitro Anti-Inflammatory Activity

Molecules ◽  
2017 ◽  
Vol 22 (1) ◽  
pp. 125 ◽  
Author(s):  
Yu Jing ◽  
Yi-Fan Zhang ◽  
Ming-Ying Shang ◽  
Guang-Xue Liu ◽  
Yao-Li Li ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Asarum Heterotropoides

scholarly journals Chemical Constituents of the Folk Medicinal Plant Argyreia acuta Lour. and Their Anti-Inflammatory Activity

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110445
Author(s):  
Yunqing Li ◽  
Xiaoyong Zhu ◽  
Liuyan Mo ◽  
Cailan Liu ◽  
Jun Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nuclear Magnetic Resonance ◽  
Medicinal Plant ◽  
Chemical Constituents ◽  
Inflammatory Activity ◽  
Two Dimensional ◽  
One Dimensional ◽  
Phytochemical Investigation ◽  
Anti Inflammatory

Phytochemical investigation of the folk medicinal plant Argyreia acuta Lour. has led to the isolation of a pair of new 3-alkylated coumarin enantiomers, (±)-acutamarin [(±)-1] and 3 known structurally related coumarins (2-4), along with 4 known flavonoids (5-8). The structures of these compounds were elucidated on the basis of extensive spectroscopic (including one-dimensional and two-dimensional nuclear magnetic resonance) analyses and by comparison of their spectral data with those reported in the literature. The absolute configurations of ( − )-1 and ( + )-1 were proposed by comparison of experimental and calculated electronic circular dichroism data. Compounds 5 and 7 exhibited in vitro anti-inflammatory activity by inhibiting the production of nitric oxide with IC50 values of 24.54 ± 0.36 μM and 10.60 ± 0.15 μM, respectively.

scholarly journals Assessment of in vitro Antioxidative and Anti-Inflammatory Effect of Caesalpinia Bonducella Seed Stabilized Silver Nanoparticles

2020 ◽  
Vol 11 (1) ◽  
pp. 693-701
Author(s):  
Gloria Jemmi Christobel R ◽  
Kasi Selvi N ◽  
Shyam Sundar J. ◽  
Abirami M.P. ◽  
Nebita Maria Jarrett ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Radical Scavenger ◽  
Frap Assay ◽  
Standard Drug ◽  
Wide Range ◽  
Caesalpinia Bonducella ◽  
Anti Inflammatory

Membrane lipid peroxidation and DNA, protein damage is mediated by free radicals, which form the basis of chronic pathological complications.AgNPs are an important class of nanomaterials for a wide range of biomedical applications. The present study endeavors in vitro antioxidant and anti-inflammatory activity of green synthesized silver nanoparticles (AgNPs) using medicinal plant extract from Caesalpinia bonducella seeds. Total flavonoid and phenolic contents were determined. The antioxidant potential of capped AgNPs was assessed using DPPH assay, Phosphomolybdenum assay, FRAP assay, metal chelating, hydrogen peroxide, and hydroxyl radical scavenging methods. In vitro anti-inflammatory assay of CB seed, AgNPs were performed against the standard drug. CB seed AgNPs possessed high flavonoid and phenol compared to aqueous CB seed extract. The antioxidant methods confirmed that the silver nanoparticles have more antioxidant activity as compared to vitamin C. The synthesized silver nanoparticles exhibited potential anti-inflammatory activity with the IC50 71.3µg/ml. Hence, this work clearly demonstrated that the coated AgNPs with CB seeds act as a potent free radical scavenger and could be considered as a potential source for anti-inflammatory drugs.

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