scholarly journals In vitro anti-urease, antioxidant, anticholinesterase, cytotoxic and in vivo anti-inflammatory potential of Satureja cuneifolia Ten.

2020 ◽  
Vol 12 (2) ◽  
pp. 222-232
Author(s):  
Turgut TASKIN ◽  
Murat DOGAN ◽  
Muhammet E. CAM ◽  
Talip SAHIN ◽  
Ismail SENKARDES
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Methanol Extracts ◽  
Aerial Parts ◽  
Direct Methanol ◽  
Anti Inflammatory

Satureja cuneifolia Ten. (wild savoury) belongs to the Lamiaceae family and is used to produce essential oil and aromatic water. This plant is also used as a condiment and herbal tea due to its stimulating, tonic and carminative effects. The in vitro antioxidant, anti-urease, anticholinesterase and cytotoxic activities of the different extracts from the plant’s aerial parts were examined. Besides, the in vivo anti-inflammatory activities of the fraction and direct methanol extracts were determined comparatively. In the current study, fraction methanol extract exhibited the strongest ABTS (52.34 mM trolox/mg extract) radical scavenging and ferric reduction (17.22 mM Fe2+/mg extract) activity. It was also found that the fraction methanol extract had stronger anti-urease (12.52%) and anticholinesterase (69.02%) activity than other extracts.  The XTT results showed that fraction methanol extract had the most cytotoxic activity on MCF-7 cell lines (39.92%).  According to the results of in vivo anti-inflammatory activity, it was found that both fraction and direct methanol extracts exhibited close and significant anti-inflammatory activity. The fact that methanol extracts have significant biological activity suggests that these may be used as a natural source in the future.

scholarly journals Src Is a Prime Target Inhibited by Celtis choseniana Methanol Extract in Its Anti-Inflammatory Action

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Han Gyung Kim ◽  
Subin Choi ◽  
Jongsung Lee ◽  
Yo Han Hong ◽  
Deok Jeong ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Inflammatory Responses ◽  
Peritoneal Macrophages ◽  
Inflammatory Activity ◽  
Poly I:C ◽  
Raw264.7 Cells ◽  
No Production ◽  
Anti Inflammatory

Celtis choseniana is the traditional plant used at Korea as a herbal medicine to ameliorate inflammatory responses. Although Celtis choseniana has been traditionally used as a herbal medicine at Korea, no systemic research has been conducted on its anti-inflammatory activity. Therefore, the present study explored an anti-inflammatory effect and its underlying molecular mechanism using Celtis choseniana methanol extract (Cc-ME) in macrophage-mediated inflammatory responses. In vitro anti-inflammatory activity of Cc-ME was evaluated using RAW264.7 cells and peritoneal macrophages stimulated by lipopolysaccharide (LPS), pam3CSK4 (Pam3), or poly(I:C). In vivo anti-inflammatory activity of Cc-ME was investigated using acute inflammatory disease mouse models, such as LPS-induced peritonitis and HCl/EtOH-induced gastritis. The molecular mechanism of Cc-ME-mediated anti-inflammatory activity was examined by Western blot analysis and immunoprecipitation using whole cell and nuclear fraction prepared from the LPS-stimulated RAW264.7 cells and HEK293 cells. Cc-ME inhibited NO production and mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and tumor necrosis factor-alpha (TNF-α) in the RAW264.7 cells and peritoneal macrophages induced by LPS, pam3, or poly(I:C) without cytotoxicity. High-performance liquid chromatography (HPLC) analysis showed that Cc-ME contained anti-inflammatory flavonoids quercetin, luteolin, and kaempferol. Among those, the content of luteolin, which showed an inhibitory effect on NO production, was highest. Cc-ME suppressed the NF-κB signaling pathway by targeting Src and interrupting molecular interactions between Src and p85, its downstream kinase. Moreover, Cc-ME ameliorated the morphological finding of peritonitis and gastritis in the mouse disease models. Therefore, these results suggest that Cc-ME exerted in vitro and in vivo anti-inflammatory activity in LPS-stimulated macrophages and mouse models of acute inflammatory diseases. This anti-inflammatory activity of Cc-ME was dominantly mediated by targeting Src in NF-κB signaling pathway during macrophage-mediated inflammatory responses.

scholarly journals In Vivo Wound Healing and In Vitro Anti-Inflammatory Activity Evaluation of Phlomis russeliana Extract Gel Formulations

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2695
Author(s):  
Mehmet Evren Okur ◽  
Ayşe Esra Karadağ ◽  
Neslihan Üstündağ Okur ◽  
Yağmur Özhan ◽  
Hande Sipahi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Activity ◽  
Wound Model ◽  
Aerial Parts ◽  
Excisional Wound ◽  
Anti Inflammatory ◽  
Cell Culture Assay ◽  
Gel Formulations

The air-dried aerial parts of Phlomis russeliana (Sims) Lag. Ex Benth. was extracted by methanol and fractionated by n-hexane, dichloromethane, and ethyl acetate, respectively. The wound healing properties of P. russeliana extract gel was evaluated using the in vivo excisional wound model using Balb-c mice. Initially, the P. russeliana methanol extract showed LOX inhibitory activity at IC50 = 23.2 µg/mL, whereas the DPPH• assay showed IC50 = 0.89 mg/mL, and the ABTS• assay showed IC50 = 0.99 mg/mL, respectively. In addition, a remarkable anti-inflammatory activity was observed in the cell culture assay. Thereafter, activity-guided fractionation was performed by LOX enzyme inhibition assays, and the structures of the two most active fractions were revealed by both GC–FID and GC/MS analyses, simultaneously. Phytol and 1-heptadecanoic acid were characterized as the active constituents. Moreover, the P. russeliana extract gel formulation was applied for in vivo tests, where the new gel formulation supported the in vitro anti-inflammatory activity findings. As a conclusion, this experimental results support the wound healing evidence based on the ethnobotanical application of Phlomis species with further potential.

scholarly journals Anti-Inflammatory Potential Evaluation (In-Vitro and In-Vivo) of Arthrophytum scoparium Aerial Part

2020 ◽  
Vol 10 (5) ◽  
pp. 213-218
Author(s):  
Sabrina Manel Kaddour ◽  
Lekhmici Arrar ◽  
Abderrahmane Baghiani
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Folk Medicine ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Traditional Use ◽  
Rat Paw Edema ◽  
Anti Inflammatory

Arthrophytum scoparium is plant commonly used in folk medicine in Algeria to treat numerous human diseases especially infectious, Rheumatism, diabetes and cancer. However, its anti-inflammatory activity and the underlying mechanisms have not been studied systematically. The present study aims to assess both the in vitro and in vivo anti-inflammatory potential and antioxidant activity of the plant. Results showed that A. scoparium chloroform extract (ChE) contains the largest amount of phenolic and flavonoid compounds. In-vitro bioassay consisted of investigating the effect of the plant extracts against protein denaturation (BSA and egg albumin). In-vivo anti-inflammatory activity was evaluated by measuring the percentage inhibition of carrageenan-induced rat paw edema after oral administration of the extracts. Results showed that inhibition of protein denaturation was maximum in case of A. scoparium crude extract (CrE) with dose dependant manner. Similarly in the in-vivo study, carrageenan induced inflammation was significantly antagonized by CrE with inhibition of 87.02% at 400 mg/kg. CrE extracts showed the highest scavenger effect against hydroxyl and hydrogen peroxide. The CrE showed potent anti-inflammatory activity in both the conditions in vivo and in vitro. In conclusion different A. scoparium extracts scavenged reactive oxygen species efficiently; it may be due to the presence of its flavonoids and polyphenols. These results support the traditional use of this plant. Keywords: Arthrophytum scoparium, polyphenols, anti-inflammatory activity, radical scavenging activity.

In-vivo anti-inflammatory activity and safety assessment of the aqueous extract of Algerian Erica arborea L. (Ericaceae) aerial parts

2021 ◽  
Vol 271 ◽  
pp. 113881
Author(s):  
Djouher Amroun ◽  
Meriem Hamoudi ◽  
Seddik Khennouf ◽  
Sabrina Boutefnouchet ◽  
Daoud Harzallah ◽  
...  
Keyword(s):  
Aqueous Extract ◽  
Safety Assessment ◽  
Inflammatory Activity ◽  
Aerial Parts ◽  
Erica Arborea ◽  
Anti Inflammatory

scholarly journals ANTI-INFLAMMATORY ACTIVITY OF CURCUMIN AND CAPSAICIN AUGMENTED IN COMBINATION

2017 ◽  
Vol 9 (6) ◽  
pp. 145
Author(s):  
Thriveni Vasanth Kumar ◽  
Manjunatha H. ◽  
Rajesh Kp
Keyword(s):  
Acetic Acid ◽  
Protective Effect ◽  
Vascular Permeability ◽  
Diclofenac Sodium ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
The Individual

Objective: Dietary curcumin and capsaicin are well known for their health beneficial potencies. The current study was done to assess the anti-inflammatory activity of curcumin, capsaicin and their combination by employing in vitro and in vivo models.Methods: We investigated the protective effect of curcumin, capsaicin and their combination using in vitro heat induced human red blood cell (HRBC) membrane stabilisation, in vivo 3% agar induced leukocyte mobilisation and acetic acid induced vascular permeability assay.Results: Curcumin, capsaicin and their combination exhibited concentration dependent protective effect against heat-induced HRBC membrane destabilisation, while combined curcumin and capsaicin restored 87.0±0.64 % membrane stability and it is found to be better than curcumin, capsaicin and diclofenac sodium (75.0±0.25. 72±0.9 and 80.0±0.31 %) protective effect. In agar suspension induced leukocyte mobilization assay, the combined curcumin and capsaicin had shown 39.5±1.58 % of inhibition compared to individual curcumin and capsaicin, which showed moderate inhibition of 16.0±3.14 and 21.6±2.17 % respectively. Besides, the combined curcumin and capsaicin had shown highly significant inhibition of acetic acid-induced vascular permeability in rats (62.0±3.14 %), whereas individual curcumin and capsaicin showed moderate inhibition of vascular permeability with 36.0±2.41 and 43.0±1.92 % respectively.Conclusion: This study demonstrates the significant anti-inflammatory property of combined curcumin and capsaicin at half of the individual concentration of curcumin and capsaicin.

Anti-Inflammatory Activity in Vitro and in Vivo of the Protein Farnesyltransferase Inhibitor Tipifarnib

2005 ◽  
Vol 317 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Xiaohua Xue ◽  
Kuei-Tai A. Lai ◽  
Jing-Feng Huang ◽  
Yin Gu ◽  
Lars Karlsson ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Farnesyltransferase Inhibitor ◽  
Protein Farnesyltransferase ◽  
Anti Inflammatory
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