scholarly journals COVID-19 serological survey using micro blood sampling

2020 ◽  
Author(s):  
Melissa M. Matthews ◽  
Tae Gyun Kim ◽  
Satoshi Shibata ◽  
Noriko Shibata ◽  
Christian Butcher ◽  
...  
Keyword(s):  
Low Cost ◽  
Positive Control ◽  
Cross Reactivity ◽  
Capillary Blood ◽  
Blood Collection ◽  
Serological Testing ◽  
Serological Survey ◽  
Local Hospital ◽  
Serum Pool ◽  
Finger Prick

AbstractDuring August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of COVID-19. We used a FDA-authorized 2-step ELISA protocol developed by the Krammer Lab (1, 2) in combination with at-home self-collection of blood samples using a custom low-cost finger prick-based capillary blood collection kit. Although our survey did not find any COVID-19 seropositive individuals among the OIST cohort, it reliably detected all positive control samples obtained from a local hospital and excluded all negatives controls. Among our controls, we found strong cross-reactivity of antibodies in samples from a serum pool from two MERS patients in the anti-SARS-CoV-2-S ELISA. Here we show that a centralized ELISA in combination with patient-based capillary blood collection using as little as one drop of blood can reliably assess the seroprevalence among communities. Anonymous sample tracking and an integrated website created a stream-lined procedure. Major parts of the workflow were automated on a liquid handler, demonstrating scalability. We anticipate this concept to serve as a prototype for reliable serological testing among larger populations.

scholarly journals COVID-19 serological survey using micro blood sampling

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Melissa M. Matthews ◽  
Tae Gyun Kim ◽  
Satoshi Shibata ◽  
Noriko Shibata ◽  
Christian Butcher ◽  
...  
Keyword(s):  
Low Cost ◽  
Serum Antibody ◽  
Positive Control ◽  
Cross Reactivity ◽  
High Serum ◽  
Capillary Blood ◽  
Blood Collection ◽  
Serological Survey ◽  
Serum Pool ◽  
Antibody Titers

AbstractDuring August 2020, we carried out a serological survey among students and employees at the Okinawa Institute of Science and Technology Graduate University (OIST), Japan, testing for the presence of antibodies against SARS-CoV-2, the causative agent of COVID-19. We used a FDA-authorized 2-step ELISA protocol in combination with at-home self-collection of blood samples using a custom low-cost finger prick-based capillary blood collection kit. Although our survey did not find any COVID-19 seropositive individuals among the OIST cohort, it reliably detected all positive control samples obtained from a local hospital and excluded all negatives controls. We found that high serum antibody titers can persist for more than 9 months post infection. Among our controls, we found strong cross-reactivity of antibodies in samples from a serum pool from two MERS patients in the anti-SARS-CoV-2-S ELISA. Here we show that a centralized ELISA in combination with patient-based capillary blood collection using as little as one drop of blood can reliably assess the seroprevalence among communities. Anonymous sample tracking and an integrated website created a stream-lined procedure. Major parts of the workflow were automated on a liquid handler, demonstrating scalability. We anticipate this concept to serve as a prototype for reliable serological testing among larger populations.

scholarly journals Self-sampling of capillary blood for serological testing of SARS-CoV-2 by COVID-19 IgG ELISA

2020 ◽  
Author(s):  
Lottie Brown ◽  
Rachel Louise Byrne ◽  
Alice Fraser ◽  
Sophie I Owen ◽  
Ana I Cubas Atienzar ◽  
...  
Keyword(s):  
Large Scale ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Capillary Blood ◽  
Blood Collection ◽  
Serological Testing ◽  
Perfect Agreement ◽  
Blood Samples ◽  
Large Scale Testing ◽  
Feasible Alternative

Serological testing is emerging as a powerful tool to progress our understanding of COVID-19 exposure, transmission and immune response. Large-scale testing is limited by the need for in-person blood collection by staff trained in venepuncture. Capillary blood self-sampling and postage to laboratories for analysis could provide a reliable alternative. Two-hundred and nine matched venous and capillary blood samples were obtained from thirty nine participants and analysed using a COVID-19 IgG ELISA to detect antibodies against SARS-CoV-2. Thirty seven out of thirty eight participants were able to self-collect an adequate sample of capillary blood (≥50 μl). Using plasma from venous blood collected in lithium heparin as the reference standard, matched capillary blood samples, collected in lithium heparin-treated tubes and on filter paper as dried blood spots, achieved a Cohen′s kappa coefficient of >0.88 (near-perfect agreement). Storage of capillary blood at room temperature for up to 7 days post sampling did not affect concordance. Our results indicate that capillary blood self-sampling is a reliable and feasible alternative to venepuncture for serological assessment in COVID-19.

scholarly journals Potential of Dried Blood Self-Sampling for CyclosporineC2Monitoring in Transplant Outpatients

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Alexander Benedikt Leichtle ◽  
Uta Ceglarek ◽  
Helmut Witzigmann ◽  
Gábor Gäbel ◽  
Joachim Thiery ◽  
...  
Keyword(s):  
Therapeutic Drug Monitoring ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Blood Samples ◽  
Drug Levels ◽  
Finger Prick

Background. Close therapeutic drug monitoring of Cyclosporine (CsA) in transplant outpatients is a favourable procedure to maintain the long-term blood drug levels within their respective narrow therapeutic ranges. Compared to basal levels (C0), CsA peak levels (C2) are more predictive for transplant rejection. However, the application ofC2levels is hampered by the precise time of blood sampling and the need of qualified personnel. Therefore, we evaluated a newC2self-obtained blood sampling in transplant outpatients using dried capillary and venous blood samples and compared the CsA levels, stability, and clinical practicability of the different procedures.Methods. 55 solid organ transplant recipients were instructed to use single-handed sampling of each 50μL capillary blood and dried blood spots by finger prick using standard finger prick devices. We used standardized EDTA-coated capillary blood collection systems and standardized filter paper WS 903. CsA was determined by LC-MS/MS. The patients and technicians also answered a questionnaire on the procedure and sample quality.Results. TheC0andC2levels from capillary blood collection systems (C0[ng/mL]:114.5±44.5;C2:578.2±222.2) and capillary dried blood (C0[ng/mL]:175.4±137.7;C2:743.1±368.1) significantly(P<.01)correlated with the drug levels of the venous blood samples (C0[ng/mL]:97.8±37.4;C2:511.2±201.5). The correlation atC0wasρcap.-ven.= 0.749, andρdried blood-ven= 0.432; atC2:  ρcap.-ven.= 0.861 andρdried blood-ven= 0.711. The patients preferred the dried blood sampling because of the more simple and less painful procedure. Additionally, the sample quality of self-obtained dried blood spots for LC-MS/MS analytics was superior to the respective capillary blood samples.Conclusions.C2self-obtained dried blood sampling can easily be performed by transplant outpatients and is therefore suitable and cost-effective for close therapeutic drug monitoring.

scholarly journals Comparison of Different Blood Collection, Sample Matrix, and Immunoassay Methods in a Prenatal Screening Setting

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jeroen L. A. Pennings ◽  
Jacqueline E. Siljee ◽  
Sandra Imholz ◽  
Sylwia Kuc ◽  
Annemieke de Vries ◽  
...  
Keyword(s):  
Prenatal Screening ◽  
Protein A ◽  
Correlation Coefficients ◽  
First Trimester ◽  
Dried Blood Spots ◽  
Maternal Blood ◽  
Blood Collection ◽  
Antibody Microarray ◽  
Sample Matrix ◽  
Finger Prick

We compared how measurements of pregnancy-associated plasma protein A (PAPP-A) and the free beta subunit of human chorionic gonadotropin (fβ-hCG) in maternal blood are influenced by different methods for blood collection, sample matrix, and immunoassay platform. Serum and dried blood spots (DBS) were obtained by venipuncture and by finger prick of 19 pregnant women. PAPP-A and fβ-hCG from serum and from DBS were measured by conventional indirect immunoassay on an AutoDELFIA platform and by antibody microarray. We compared methods based on the recoveries for both markers as well as marker levels correlations across samples. All method comparisons showed high correlations for both marker concentrations. Recovery levels of PAPP-A from DBS were 30% lower, while those of fβ-hCG from DBS were 50% higher compared to conventional venipuncture serum. The recoveries were not affected by blood collection or immunoassay method. The high correlation coefficients for both markers indicate that DBS from finger prick can be used reliably in a prenatal screening setting, as a less costly and minimally invasive alternative for venipuncture serum, with great logistical advantages. Additionally, the use of antibody arrays will allow for extending the number of first trimester screening markers on maternal and fetal health.

scholarly journals Rapid Antibody Selection Using Surface Plasmon Resonance for High-Speed and Sensitive Hazelnut Lateral Flow Prototypes

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 130 ◽  
Author(s):  
Georgina Ross ◽  
Maria Bremer ◽  
Jan Wichers ◽  
Aart van Amerongen ◽  
Michel Nielen
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Speed ◽  
Selection Process ◽  
Low Cost ◽  
Real Life ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Label Free

Lateral Flow Immunoassays (LFIAs) allow for rapid, low-cost, screening of many biomolecules such as food allergens. Despite being classified as rapid tests, many LFIAs take 10–20 min to complete. For a really high-speed LFIA, it is necessary to assess antibody association kinetics. By using a label-free optical technique such as Surface Plasmon Resonance (SPR), it is possible to screen crude monoclonal antibody (mAb) preparations for their association rates against a target. Herein, we describe an SPR-based method for screening and selecting crude anti-hazelnut antibodies based on their relative association rates, cross reactivity and sandwich pairing capabilities, for subsequent application in a rapid ligand binding assay. Thanks to the SPR selection process, only the fast mAb (F-50-6B12) and the slow (S-50-5H9) mAb needed purification for labelling with carbon nanoparticles to exploit high-speed LFIA prototypes. The kinetics observed in SPR were reflected in LFIA, with the test line appearing within 30 s, almost two times faster when F-50-6B12 was used, compared with S-50-5H9. Additionally, the LFIAs have demonstrated their future applicability to real life samples by detecting hazelnut in the sub-ppm range in a cookie matrix. Finally, these LFIAs not only provide a qualitative result when read visually, but also generate semi-quantitative data when exploiting freely downloadable smartphone apps.

scholarly journals LAMP assay coupled with CRISPR/Cas12a system for portable detection of African swine fever virus

2021 ◽  
Author(s):  
Bo YANG ◽  
zhengwang shi ◽  
Yuan Ma ◽  
Lijuan Wang ◽  
Liyan Cao ◽  
...  
Keyword(s):  
Real Time ◽  
Detection System ◽  
African Swine Fever Virus ◽  
Low Cost ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Cross Reactivity ◽  
Clinical Samples ◽  
Portable Detection ◽  
Real Time Qpcr

African swine fever (ASF) is one of the most severe infectious diseases of pigs. In this study, a LAMP assay coupled with the CRISPR Cas12a system was established in one tube for the detection of the ASFV p72 gene. The single-strand DNA-fluorophore-quencher (ssDNA-FQ) reporters and CRISPR-derived RNA (crRNAs) were screened and selected for the CRISPR detection system. In combination with LAMP amplification assay, the detection limit for the LAMP-CRISPR assay can reach 7 copies/μl of p72 gene per reaction. Furthermore, this method displays no cross-reactivity with other porcine DNA or RNA viruses. The performance of the LAMP-CRISPR assay was compared with real-time qPCR tests for clinical samples, a good consistency between the LAMP-CRISPR assay and real-time qPCR was observed. In the current study, a LAMP coupled with the CRISPR detection method was developed. The method shed a light on the convenient, portable, low cost, highly sensitive and specific detection of ASFV, demonstrating a great application potential for monitoring on-site ASFV in the field.

Quantitative metabolomics comparison of traditional blood draws and TAP capillary blood collection

Metabolomics ◽  
2018 ◽  
Vol 14 (7) ◽  
Author(s):  
Alexis Catala ◽  
Rachel Culp-Hill ◽  
Travis Nemkov ◽  
Angelo D’Alessandro
Keyword(s):  
Capillary Blood ◽  
Blood Collection ◽  
Quantitative Metabolomics

Ferritin concentrations in plasma from capillary (finger prick) blood and venous blood compared.

1983 ◽  
Vol 29 (5) ◽  
pp. 871-873 ◽  
Author(s):  
L A Mejía ◽  
F E Viteri
Keyword(s):  
Blood Plasma ◽  
Close Association ◽  
Venous Blood ◽  
Capillary Blood ◽  
Ferritin Concentration ◽  
Antecubital Vein ◽  
Finger Prick ◽  
Adults And Children ◽  
Venous Plasma

Abstract Investigating the feasibility and validity of determining plasma ferritin concentration in blood obtained by finger prick, we studied 29 adults (ages 21-49 years) and 35 children (ages 14-66 months). Blood was sampled simultaneously in the same subject from both the antecubital vein (venous blood) and by finger pricking (capillary blood). The plasma was obtained by centrifugation. Ferritin concentration was determined by immunoradiometric analysis. Ferritin concentration in plasma from capillary blood was significantly higher than in venous plasma (p less than 0.01). This difference was more marked in children. The correlation between ferritin from the two blood sources was highly significant (r2 = 0.945 and 0.994 for samples from adults and children, respectively), and the slopes of the respective regression lines in both children and adults were significantly different from 1 (p less than 0.0001). We conclude that, despite the close association between the two procedures, the determination of ferritin concentration in capillary blood plasma overestimates the concentration of ferritin in venous blood plasma.

Monoject Samplette capillary blood container with serum separator evaluated for collection of specimens for therapeutic drug assays and common clinical-chemical tests.

1986 ◽  
Vol 32 (3) ◽  
pp. 523-526 ◽  
Author(s):  
M Landt ◽  
L L Norling ◽  
M Steelman ◽  
C H Smith
Keyword(s):  
Pediatric Patients ◽  
Capillary Blood ◽  
Therapeutic Drug ◽  
Blood Collection ◽  
Therapeutic Drugs ◽  
Clinical Chemical ◽  
Drug Assays ◽  
Separator Material ◽  
Clinically Significant

Abstract The Monoject Samplette (Sherwood) capillary serum-separator tube was evaluated for use in pediatric capillary blood collection. When patients' values for eight common clinical-chemical tests and five therapeutic drugs were compared with values from specimens concomitantly collected in plain Caraway tubes, only chloride and total CO2 were significantly different. The chloride differences (range 0-2 mmol/L) were considered to be clinically insignificant. Higher CO2 values in Samplette specimens were apparently ascribable to decreased loss to the atmosphere. Samplette values for therapeutic drugs were higher than corresponding Caraway values, but only the differences for digoxin were judged to be clinically significant. Both recoverable serum and the incidence of hemolysis were lower in Samplette specimens than in Caraway specimens. Storage of serum over the clots (with separator material interposed) in Samplettes for 24 h had no clinically significant effect on results for glucose or potassium. Storage of specimens for as long as 24 h had no effect on theophylline, phenytoin, and gentamicin concentrations, but phenobarbital reproducibly decreased after 24 h. We conclude that the Samplette serum-separator tube is suitable for the collection of capillary blood for many of the chemical tests commonly ordered for pediatric patients.

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