Temporal Microbial Community Dynamics within a Unique Acid Saline Lake

AbstractLake Magic is one of the acidic hypersaline lakes (ca. 1 km in diameter) present within the Yilgarn Craton in WA. This unique lake exhibits extremely low pH (<1.6) coupled to very high salinity (32% TDS) with the highest concentration of aluminium (1774 mg/L) and silica (510 mg/L) in the world. Previous studies on Lake Magic diversity has revealed that the lake hosts acidophilic, acidotolerant, halophilic and halotolerant bacterial species. These studies provide indicators of the population residing within the lake. However, they do not emphasize the survival mechanisms adopted by the resident microorganisms and how the diversity of microbial populations residing within the lake changes during the dynamic stages of flooding, evapo-concentration and desiccation. We have studied the bacterial and fungal diversity in Lake Magic via amplicon sequencing and functional analysis through different stages of the lake in a span of one year, in the salt and sediment layer. Our results highlight that the diversity in Lake Magic is strongly driven by the pH and salt concentrations at different stages of the lake. The microbial community becomes more specialised in specific functions during more extreme stages. This also suggests that microbial interactions are involved in stabilising the ecosystem and is responsible for the resistance and resilience of these communities as the interactions of these microbes create a safe haven for other microbes to survive during more extreme stages.

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Temporal Microbial Community Dynamics Within a Unique Acid Saline Lake

Frontiers in Microbiology ◽

10.3389/fmicb.2021.649594 ◽

2021 ◽

Vol 12 ◽

Author(s):

Noor-Ul-Huda Ghori ◽

Michael J. Wise ◽

Andrew S. Whiteley

Keyword(s):

Microbial Community ◽

Environmental Conditions ◽

Fungal Diversity ◽

Community Dynamics ◽

Bacterial Species ◽

Amplicon Sequencing ◽

Fungal Species ◽

Hypersaline Lake ◽

Microbial Community Dynamics ◽

Abundance And Diversity

Lake Magic is an extremely acidic, hypersaline lake found in Western Australia, with the highest concentrations of aluminum and silica in the world. Previous studies of Lake Magic diversity have revealed that the lake hosts acid- and halotolerant bacterial and fungal species. However, they have not canvassed microbial population dynamics across flooding, evapo-concentration and desiccation stages. In this study, we used amplicon sequencing and potential function prediction on sediment and salt mat samples. We observed that the bacterial and fungal diversity in Lake Magic is strongly driven by carbon, temperature, pH and salt concentrations at the different stages of the lake. We also saw that the fungal diversity decreased as the environmental conditions became more extreme. However, prokaryotic diversity was very dynamic and bacteria dominated archaeal species, both in abundance and diversity, perhaps because bacteria better tolerate the extreme variation in conditions. Bacterial species diversity was the highest during early flooding stage and decreased during more stressful conditions. We observed an increase in acid tolerant and halotolerant species in the sediment, involved in functions such as sulfur and iron metabolism, i.e., species involved in buffering the external environment. Thus, due to activity within the microbial community, the environmental conditions in the sediment do not change to the same degree as conditions in the salt mat, resulting in the sediment becoming a safe haven for microbes, which are able to thrive during the extreme conditions of the evapo-concentration and desiccation stages.

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One-year monitoring of meta-cleavage dioxygenase gene expression and microbial community dynamics reveals the relevance of subfamily I.2.C extradiol dioxygenases in hypoxic, BTEX-contaminated groundwater

Systematic and Applied Microbiology ◽

10.1016/j.syapm.2013.03.008 ◽

2013 ◽

Vol 36 (5) ◽

pp. 339-350 ◽

Cited By ~ 18

Author(s):

András Táncsics ◽

Milán Farkas ◽

Sándor Szoboszlay ◽

István Szabó ◽

József Kukolya ◽

...

Keyword(s):

Gene Expression ◽

Microbial Community ◽

Community Dynamics ◽

Contaminated Groundwater ◽

Microbial Community Dynamics ◽

Dioxygenase Gene ◽

One Year ◽

Extradiol Dioxygenases

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Quorum quenching, biological characteristics, and microbial community dynamics as key factors for combating fouling of membrane bioreactors

npj Clean Water ◽

10.1038/s41545-021-00110-9 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Syed Salman Ali Shah ◽

Luigi De Simone ◽

Giuseppe Bruno ◽

Hyeona Park ◽

Kibaek Lee ◽

...

Keyword(s):

Microbial Community ◽

Membrane Fouling ◽

Community Dynamics ◽

Bacterial Species ◽

Quorum Quenching ◽

Community Change ◽

Membrane Bioreactors ◽

Solid Retention Time ◽

Fouling Control ◽

Microbial Community Dynamics

AbstractMembrane fouling is a major challenge in membrane bioreactors (MBRs) for wastewater treatment. This study investigates the effects of disturbance and solid retention time (SRT) on quorum-quenching (QQ) MBRs relative to antifouling efficacy and microbial community change. The fouling rate increases with the applied disturbance at a short SRT, counteracting the antifouling effect of QQ; however, it decreases with QQ at a long SRT. The microbial community appears to be responsible for such MBR behaviors. Several bacterial species belonging to the biofilm-forming group are dominant after disturbance, resulting in substantive membrane fouling. However, the balance between the bacterial species plays a key role in MBR fouling propensity when stabilized. Koflera flava becomes dominant with QQ, leading to reduced membrane fouling. QQ makes the MBR microbial community more diverse, while lowering its richness. QQ with long SRT would be a favorable operational strategy for effective MBR fouling control.

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Strengths and Limitations of 16S rRNA Gene Amplicon Sequencing in Revealing Temporal Microbial Community Dynamics

PLoS ONE ◽

10.1371/journal.pone.0093827 ◽

2014 ◽

Vol 9 (4) ◽

pp. e93827 ◽

Cited By ~ 246

Author(s):

Rachel Poretsky ◽

Luis M. Rodriguez-R ◽

Chengwei Luo ◽

Despina Tsementzi ◽

Konstantinos T. Konstantinidis

Keyword(s):

Microbial Community ◽

16S Rrna ◽

16S Rrna Gene ◽

Community Dynamics ◽

Amplicon Sequencing ◽

Rrna Gene ◽

Microbial Community Dynamics

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Microbial community dynamics reflect reactor stability during the anaerobic digestion of a very high strength and sulfate-rich vinasse

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.5449 ◽

2017 ◽

Vol 93 (4) ◽

pp. 975-984 ◽

Cited By ~ 6

Author(s):

Janet Jiménez ◽

Ernesto L Barrera ◽

Jo De Vrieze ◽

Nico Boon ◽

Steven DeMeester ◽

...

Keyword(s):

Microbial Community ◽

Anaerobic Digestion ◽

Community Dynamics ◽

High Strength ◽

Microbial Community Dynamics ◽

Reactor Stability ◽

Very High

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Dynamics of a Perturbed Microbial Community during Thermophilic Anaerobic Digestion of Chemically Defined Soluble Organic Compounds

Microorganisms ◽

10.3390/microorganisms6040105 ◽

2018 ◽

Vol 6 (4) ◽

pp. 105 ◽

Cited By ~ 4

Author(s):

Luka Šafarič ◽

Sepehr Shakeri Yekta ◽

Tong Liu ◽

Bo Svensson ◽

Anna Schnürer ◽

...

Keyword(s):

Microbial Community ◽

Anaerobic Digestion ◽

Community Dynamics ◽

Bacterial Species ◽

Stable Process ◽

Archaeal Community ◽

Amplicon Sequencing ◽

Rrna Genes ◽

Methane Formation ◽

Process Performance

Knowledge of microbial community dynamics in relation to process perturbations is fundamental to understand and deal with the instability of anaerobic digestion (AD) processes. This study aims to investigate the microbial community structure and function of a thermophilic AD process, fed with a chemically defined substrate, and its association with process performance stability. Next generation amplicon sequencing of 16S ribosomal RNA (rRNA) genes revealed that variations in relative abundances of the predominant bacterial species, Defluviitoga tunisiensis and Anaerobaculum hydrogeniformans, were not linked to the process performance stability, while dynamics of bacterial genera of low abundance, Coprothermobacter and Defluviitoga (other than D. tunisiensis), were associated with microbial community function and process stability. A decrease in the diversity of the archaeal community was observed in conjunction with process recovery and stable performance, implying that the high abundance of specific archaeal group(s) contributed to the stable AD. Dominance of hydrogenotrophic Methanoculleus particularly corresponded to an enhanced microbial acetate and propionate turnover capacity, whereas the prevalence of hydrogenotrophic Methanothermobacter and acetoclastic Methanosaeta was associated with instable AD. Acetate oxidation via syntrophic interactions between Coprothermobacter and Methanoculleus was potentially the main methane-formation pathway during the stable process. We observed that supplementation of Se and W to the medium improved the propionate turnover by the thermophilic consortium. The outcomes of our study provided insights into the community dynamics and trace element requirements in relation to the process performance stability of thermophilic AD.

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