Effects of conjugated linoleic acid on proliferation and differentiation of bovine intramuscular preadipocyte in vitro

Mapping Intimacies ◽

10.1101/2021.01.30.428933 ◽

2021 ◽

Author(s):

Rong Wan ◽

Qingxiang Meng ◽

Zhou Zhenming ◽

Wu hao

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Intramuscular Fat ◽

Fat Content ◽

Proliferation And Differentiation ◽

Cell Proportion ◽

Intramuscular Fat Content

ABSTRACTConjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to increase intramuscular fat content in vivo and stimulate adipogenesis in intramuscular preadipocytes in vitro in pig. Unfortunately, there is little data to evaluate the effect of CLA on proliferation and differentiation of bovine intramuscular preadipocytes. This study investigated the regulation by CLA in proliferation and differentiation of bovine intramuscular preadipocytes. The results demonstrated that CLA significantly induced the expression of PPARγ and C/EBPα mRNA of bovine intramuscular preadipocytes as well as the accumulation of lipid in cultured intramuscular preadipocytes. Additionally, CLA significantly decreased the cell proportion of phase G0/G1, and remarkably increased the proportion of phase S+G2/M. Collectively, these results suggest that CLA promotes bovine intramuscular preadipocyte proliferation and differentiation.

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Prolonged dietary treatment with conjugated linoleic acid stimulates porcine muscle peroxisome proliferator activated receptor gamma and glutamine-fructose aminotransferase gene expression in vivo

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0280079 ◽

2002 ◽

Vol 28 (2) ◽

pp. 79-86 ◽

Cited By ~ 47

Author(s):

WJ Meadus ◽

R MacInnis ◽

ME Dugan

Keyword(s):

Fatty Acid ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Intramuscular Fat ◽

Fat Content ◽

The Body ◽

Peroxisome Proliferator ◽

Mrna Levels ◽

Fatty Acid Binding ◽

Intramuscular Fat Content

Peroxisome proliferator activated receptors (PPARs) represent a family of DNA binding proteins that are activated by a variety of dietary and endogenous fatty acids. The PPAR proteins are expressed throughout the body and are the target of a variety of lipidaemic and insulin sensitizing drugs. Conjugated linoleic acid (CLA) is a collective name for octadecadienoic acid isomers with conjugated double bonds, which can also act as ligands for some of the PPAR family. To gain better understanding of the long-term effects of PPAR activation, CLA was fed at 11 g/kg of feed for 45 days to castrated male pigs (barrows). These barrows had a significant repartitioning of subcutaneous fat to lean tissue in the carcass: fat was reduced by 9 x 2% and lean muscle was increased by 3 x 5%, but intramuscular fat content was also increased by 14% (P<0 x 05). PPARgamma, glutamine-fructose aminotransferase (GFAT), adipocyte fatty acid binding protein (AFABP), but not PPARalpha mRNA levels were significantly increased (P<0 x 05) in the CLA-fed pigs. The increased expression of PPARgamma and AFABP indicates that CLA induced the development of preadipocytes from stromal-vascular (s-v) stem cells to promote intramuscular fat content. The increase in the expression of GFAT mRNA indicates that the glucose supply of the muscle cells had been increased with the CLA diet, possibly sparing intramuscular fatty acid reserves.

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Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid

British Journal Of Nutrition ◽

10.1017/s0007114510003181 ◽

2010 ◽

Vol 105 (1) ◽

pp. 1-9 ◽

Cited By ~ 15

Author(s):

Weijing Zhong ◽

Zongyong Jiang ◽

Chuntian Zheng ◽

Yingcai Lin ◽

Lin Yang ◽

...

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Intramuscular Fat ◽

Fat Content ◽

Acid Oxidation ◽

Longissimus Muscle ◽

Finishing Pigs ◽

Large White ◽

Proteome Changes ◽

Intramuscular Fat Content

The present experiment was conducted to determine proteome changes in Longissimus muscle of finishing pigs fed conjugated linoleic acid (CLA), in association with alteration of intramuscular fat content. Previously, seventy-two Duroc × Landrace × Large White gilts (approximately 60 kg) had been fed maize–soyabean meal-based diets with 0, 12·5 and 25 g CLA/kg diet. The CLA contained 369·1 mg/g cis-9, trans-11 CLA, 374·6 mg/g trans-10, cis-12 CLA and 53·7 mg/g other isomers. Six pigs per treatment were slaughtered when they reached a body weight of approximately 100 kg. Data published from a previous experiment demonstrated that supplementation with 12·5 or 25 g CLA/kg diet increased intramuscular fat content (P < 0·05). The present study investigated the proteome changes in Longissimus muscle of control and pigs supplemented with 25 g CLA/kg diet. CLA significantly influenced the abundance of proteins related to energy metabolism, fatty acid oxidation and synthesis, amino acid metabolism, defence, transport and other miscellaneous processes (P < 0·05). The increase in intramuscular fat content was positively correlated with the increased abundance of carbonic anhydrase 3 and aspartate aminotransferase (P < 0·05). We suggest that the proteome changes in Longissimus muscle contributed to greater intramuscular lipid content in CLA-supplemented pigs.

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Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria

Applied and Environmental Microbiology ◽

10.1128/aem.70.8.4635-4641.2004 ◽

2004 ◽

Vol 70 (8) ◽

pp. 4635-4641 ◽

Cited By ~ 59

Author(s):

E. Rosberg-Cody ◽

R. P. Ross ◽

S. Hussey ◽

C. A. Ryan ◽

B. P. Murphy ◽

...

Keyword(s):

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Bifidobacterium Bifidum ◽

Genomic Diversity ◽

Gas Liquid Chromatography ◽

Fecal Material ◽

Single Strain ◽

Different Strains

ABSTRACT This study was designed to isolate different strains of the genus Bifidobacterium from the fecal material of neonates and to assess their ability to produce the cis-9, trans-11 conjugated linoleic acid (CLA) isomer from free linoleic acid. Fecal material was collected from 24 neonates aged between 3 days and 2 months in a neonatal unit (Erinville Hospital, Cork, Ireland). A total of 46 isolates from six neonates were confirmed to be Bifidobacterium species based on a combination of the fructose-6-phosphate phosphoketolase assay, RAPD [random(ly) amplified polymorphic DNA] PCR, pulsed-field gel electrophoresis (PFGE), and partial 16S ribosomal DNA sequencing. Interestingly, only 1 of the 11 neonates that had received antibiotic treatment produced bifidobacteria. PFGE after genomic digestion with the restriction enzyme XbaI demonstrated that the bifidobacteria population displayed considerable genomic diversity among the neonates, with each containing between one and five dominant strains, whereas 11 different macro restriction patterns were obtained. In only one case did a single strain appear in two neonates. All genetically distinct strains were then screened for CLA production after 72 h of incubation with 0.5 mg of free linoleic acid ml−1 by using gas-liquid chromatography. The most efficient producers belonged to the species Bifidobacterium breve, of which two different strains converted 29 and 27% of the free linoleic acid to the cis-9, trans-11 isomer per microgram of dry cells, respectively. In addition, a strain of Bifidobacterium bifidum showed a conversion rate of 18%/μg dry cells. The ability of some Bifidobacterium strains to produce CLA could be another human health-promoting property linked to members of the genus, given that this metabolite has demonstrated anticarcinogenic activity in vitro and in vivo.

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The link between supplementary tannin level and conjugated linoleic acid (CLA) formation in ruminants: A meta-analysis

10.1101/612523 ◽

2019 ◽

Cited By ~ 1

Author(s):

Rayudika Aprilia Patindra Purba ◽

Pramote Paengkoum ◽

Siwaporn Paengkoum

Keyword(s):

Fatty Acid ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Human Health ◽

Meta Analysis ◽

Problem Solver ◽

Health Issues ◽

Dietary Treatments

AbstractThis meta-analysis was conducted to predict and assert a way to discover conjugated linoleic acid (CLA) formation in ruminant-derived products as problem solver of human health issues threated by plant-containing tannins. The objective was to expound, to compare, and to confirm the efficiency of tannins cultivating CLA formation whether using in vitro and/or in vivo study. A database was created using the ruminants with selectively 26 experiments comprising 683 dietary treatments as explained in vitro and in vivo methods that were applied as a statistical SAS 9.4 tool. Basically, increasing level of tannins leaded to an underlying decrease in CLA formation (p<0.001), initially at predicting coefficient determination R2=0.193, R2=0.929, and R2=0.549 for CLA in vitro, in vivo of CLA milk shift, and in vivo of CLA meat precipitation, respectively. In vitro may accurately predict to the in vivo observation. Unfortunately, there were no relationship in vitro towards in vivo observation (R2<0.1). It indicated to be difficult to predict CLA from in vitro to in vivo separately situations. According to all studies, the level of tannin’s utilization for inhibiting biohydrogenation was not exceedingly >50 g/kg DM recommended. Secondly, the in vivo method was more suitable for directly observation that concerned in fatty acid transformation.

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