scholarly journals Prolonged dietary treatment with conjugated linoleic acid stimulates porcine muscle peroxisome proliferator activated receptor gamma and glutamine-fructose aminotransferase gene expression in vivo

2002 ◽  
Vol 28 (2) ◽  
pp. 79-86 ◽  
Author(s):  
WJ Meadus ◽  
R MacInnis ◽  
ME Dugan
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intramuscular Fat ◽  
Fat Content ◽  
The Body ◽  
Peroxisome Proliferator ◽  
Mrna Levels ◽  
Fatty Acid Binding ◽  
Intramuscular Fat Content

Peroxisome proliferator activated receptors (PPARs) represent a family of DNA binding proteins that are activated by a variety of dietary and endogenous fatty acids. The PPAR proteins are expressed throughout the body and are the target of a variety of lipidaemic and insulin sensitizing drugs. Conjugated linoleic acid (CLA) is a collective name for octadecadienoic acid isomers with conjugated double bonds, which can also act as ligands for some of the PPAR family. To gain better understanding of the long-term effects of PPAR activation, CLA was fed at 11 g/kg of feed for 45 days to castrated male pigs (barrows). These barrows had a significant repartitioning of subcutaneous fat to lean tissue in the carcass: fat was reduced by 9 x 2% and lean muscle was increased by 3 x 5%, but intramuscular fat content was also increased by 14% (P<0 x 05). PPARgamma, glutamine-fructose aminotransferase (GFAT), adipocyte fatty acid binding protein (AFABP), but not PPARalpha mRNA levels were significantly increased (P<0 x 05) in the CLA-fed pigs. The increased expression of PPARgamma and AFABP indicates that CLA induced the development of preadipocytes from stromal-vascular (s-v) stem cells to promote intramuscular fat content. The increase in the expression of GFAT mRNA indicates that the glucose supply of the muscle cells had been increased with the CLA diet, possibly sparing intramuscular fatty acid reserves.

scholarly journals Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs

2005 ◽  
Vol 53 (4) ◽  
pp. 87-94
Author(s):  
Renata Mikolášová ◽  
Tomáš Urban
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Leptin Receptor ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content

The leptin (LEP-HinfI), leptin receptor (LEPR-HpaII) and heart fatty acid binding protein (H-FABP-HinfI) genes and their genotypes combination (LEP-HinfI *LEPR-HpaII) were tested for associations with the pH1, pH24, myoglobin content (mg/100 g), intramuscular fat content (%) and remission (%). The genotypes were determined in Large White, Landrace and Duroc breeds (n = 106, 56 and 4, respectively). The allele frequencies were: LEP-HinfI: C = 0.133 T = 0.867; LEPR-HpaII: A = 0.331 B = 0.669; H-FABP-HinfI: H = 0.745 h = 0.255. The populations of breeds were in the genetic equilibrium according to the χ2 test in the tested loci. The combinations of LEP-HinfI and LEPR-HpaII were significantly associated with the pH24 and remission. The H-FABP-HinfI locus was significantly associated with intramuscular fat content.

scholarly journals Effects of conjugated linoleic acid on proliferation and differentiation of bovine intramuscular preadipocyte in vitro

2021 ◽  
Author(s):  
Rong Wan ◽  
Qingxiang Meng ◽  
Zhou Zhenming ◽  
Wu hao
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Proliferation And Differentiation ◽  
Cell Proportion ◽  
Intramuscular Fat Content

ABSTRACTConjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to increase intramuscular fat content in vivo and stimulate adipogenesis in intramuscular preadipocytes in vitro in pig. Unfortunately, there is little data to evaluate the effect of CLA on proliferation and differentiation of bovine intramuscular preadipocytes. This study investigated the regulation by CLA in proliferation and differentiation of bovine intramuscular preadipocytes. The results demonstrated that CLA significantly induced the expression of PPARγ and C/EBPα mRNA of bovine intramuscular preadipocytes as well as the accumulation of lipid in cultured intramuscular preadipocytes. Additionally, CLA significantly decreased the cell proportion of phase G0/G1, and remarkably increased the proportion of phase S+G2/M. Collectively, these results suggest that CLA promotes bovine intramuscular preadipocyte proliferation and differentiation.

The adipocyte fatty acid-binding protein locus: characterization and association with intramuscular fat content in pigs

1998 ◽  
Vol 9 (12) ◽  
pp. 1022-1026 ◽  
Author(s):  
Frans Gerbens ◽  
Aswin Jansen ◽  
Anton J.M. van Erp ◽  
Frank Harders ◽  
Theo H.E. Meuwissen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Protein Locus ◽  
Intramuscular Fat Content

scholarly journals Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid

2010 ◽  
Vol 105 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Weijing Zhong ◽  
Zongyong Jiang ◽  
Chuntian Zheng ◽  
Yingcai Lin ◽  
Lin Yang ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Acid Oxidation ◽  
Longissimus Muscle ◽  
Finishing Pigs ◽  
Large White ◽  
Proteome Changes ◽  
Intramuscular Fat Content

The present experiment was conducted to determine proteome changes in Longissimus muscle of finishing pigs fed conjugated linoleic acid (CLA), in association with alteration of intramuscular fat content. Previously, seventy-two Duroc × Landrace × Large White gilts (approximately 60 kg) had been fed maize–soyabean meal-based diets with 0, 12·5 and 25 g CLA/kg diet. The CLA contained 369·1 mg/g cis-9, trans-11 CLA, 374·6 mg/g trans-10, cis-12 CLA and 53·7 mg/g other isomers. Six pigs per treatment were slaughtered when they reached a body weight of approximately 100 kg. Data published from a previous experiment demonstrated that supplementation with 12·5 or 25 g CLA/kg diet increased intramuscular fat content (P < 0·05). The present study investigated the proteome changes in Longissimus muscle of control and pigs supplemented with 25 g CLA/kg diet. CLA significantly influenced the abundance of proteins related to energy metabolism, fatty acid oxidation and synthesis, amino acid metabolism, defence, transport and other miscellaneous processes (P < 0·05). The increase in intramuscular fat content was positively correlated with the increased abundance of carbonic anhydrase 3 and aspartate aminotransferase (P < 0·05). We suggest that the proteome changes in Longissimus muscle contributed to greater intramuscular lipid content in CLA-supplemented pigs.

Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal

Meat Science ◽  
2007 ◽  
Vol 75 (1) ◽  
pp. 44-52 ◽  
Author(s):  
Cristina M.M. Alfaia ◽  
Matilde L.F. Castro ◽  
Susana I.V. Martins ◽  
Ana P.V. Portugal ◽  
Susana P.A. Alves ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Acid Composition ◽  
Nutritional Value ◽  
Intramuscular Fat

scholarly journals Trans-10, cis-12 conjugated linoleic acid reduces milk fat content and lipogenic gene expression in the mammary gland of sows without altering litter performance

2019 ◽  
Vol 123 (6) ◽  
pp. 610-618 ◽  
Author(s):  
E. C. Sandri ◽  
K. J. Harvatine ◽  
D. E. Oliveira
Keyword(s):  
Gene Expression ◽  
Mammary Gland ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Fat Content ◽  
Lipogenic Gene ◽  
Lipogenic Genes ◽  
Lipogenic Gene Expression

AbstractTrans-10, cis-12 conjugated linoleic acid (CLA) decreases milk fat synthesis in lactating sows and involves, at least in part, the down-regulation of lipogenic genes. The objective was to evaluate the effect of CLA on milk composition and lipogenic gene expression. Twenty multiparous sows were randomly assigned to one of the two treatments for 18 d (from day 7 to day 25 of lactation): (1) control (no CLA added) and (2) 1 % of CLA mixed into the ration. CLA treatment decreased milk fat and protein content by 20 % (P = 0·004) and 11 % (P = 0·0001), respectively. However, piglet weight did not differ between treatments (P = 0·60). Dietary CLA increased the concentration of SFA in milk fat by 16 % (P < 0·0001) and decreased MUFA by 17·6 % (P < 0·0001). In the mammary gland, CLA reduced gene expression of acetyl-CoA carboxylase-α by 37 % (P = 0·003), fatty acid synthase by 64 % (P = 0·002), stearoyl-CoA desaturase 1 by 52 % (P = 0·003), lipoprotein lipase by 26 % (P = 0·03), acyl glycerol phosphate acyltransferase 6 by 15 % (P = 0·02) and diacylglycerol acyltransferase 1 by 27 % (P = 0·02), whereas the expression of fatty acid binding protein 3 was not altered by CLA treatment (P = 0·09). Mammary expression of casein-β and α-lactalbumin was reduced by CLA by 68 % (P = 0·0004) and 62 % (P = 0·005), respectively. Additionally, CLA had no effect on the expression of lipogenic genes evaluated in adipose tissue. In summary, CLA reduced milk fat content without negatively affecting litter performance and it affected mammary expression of genes involved in all lipogenic pathways studied.

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