scholarly journals The link between supplementary tannin level and conjugated linoleic acid (CLA) formation in ruminants: A meta-analysis

2019 ◽  
Author(s):  
Rayudika Aprilia Patindra Purba ◽  
Pramote Paengkoum ◽  
Siwaporn Paengkoum
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Human Health ◽  
Meta Analysis ◽  
Problem Solver ◽  
Health Issues ◽  
Dietary Treatments

AbstractThis meta-analysis was conducted to predict and assert a way to discover conjugated linoleic acid (CLA) formation in ruminant-derived products as problem solver of human health issues threated by plant-containing tannins. The objective was to expound, to compare, and to confirm the efficiency of tannins cultivating CLA formation whether using in vitro and/or in vivo study. A database was created using the ruminants with selectively 26 experiments comprising 683 dietary treatments as explained in vitro and in vivo methods that were applied as a statistical SAS 9.4 tool. Basically, increasing level of tannins leaded to an underlying decrease in CLA formation (p<0.001), initially at predicting coefficient determination R2=0.193, R2=0.929, and R2=0.549 for CLA in vitro, in vivo of CLA milk shift, and in vivo of CLA meat precipitation, respectively. In vitro may accurately predict to the in vivo observation. Unfortunately, there were no relationship in vitro towards in vivo observation (R2<0.1). It indicated to be difficult to predict CLA from in vitro to in vivo separately situations. According to all studies, the level of tannin’s utilization for inhibiting biohydrogenation was not exceedingly >50 g/kg DM recommended. Secondly, the in vivo method was more suitable for directly observation that concerned in fatty acid transformation.

scholarly journals Conjugated linoleic acid: A potent fatty acid linked to animal and human health

2015 ◽  
Vol 57 (13) ◽  
pp. 2737-2748 ◽  
Author(s):  
Parisa Shokryazdan ◽  
Mohamed Ali Rajion ◽  
Goh Yong Meng ◽  
Liang Juan Boo ◽  
Mahdi Ebrahimi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Human Health

scholarly journals Mining the Microbiota of the Neonatal Gastrointestinal Tract for Conjugated Linoleic Acid-Producing Bifidobacteria

2004 ◽  
Vol 70 (8) ◽  
pp. 4635-4641 ◽  
Author(s):  
E. Rosberg-Cody ◽  
R. P. Ross ◽  
S. Hussey ◽  
C. A. Ryan ◽  
B. P. Murphy ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Bifidobacterium Bifidum ◽  
Genomic Diversity ◽  
Gas Liquid Chromatography ◽  
Fecal Material ◽  
Single Strain ◽  
Different Strains

ABSTRACT This study was designed to isolate different strains of the genus Bifidobacterium from the fecal material of neonates and to assess their ability to produce the cis-9, trans-11 conjugated linoleic acid (CLA) isomer from free linoleic acid. Fecal material was collected from 24 neonates aged between 3 days and 2 months in a neonatal unit (Erinville Hospital, Cork, Ireland). A total of 46 isolates from six neonates were confirmed to be Bifidobacterium species based on a combination of the fructose-6-phosphate phosphoketolase assay, RAPD [random(ly) amplified polymorphic DNA] PCR, pulsed-field gel electrophoresis (PFGE), and partial 16S ribosomal DNA sequencing. Interestingly, only 1 of the 11 neonates that had received antibiotic treatment produced bifidobacteria. PFGE after genomic digestion with the restriction enzyme XbaI demonstrated that the bifidobacteria population displayed considerable genomic diversity among the neonates, with each containing between one and five dominant strains, whereas 11 different macro restriction patterns were obtained. In only one case did a single strain appear in two neonates. All genetically distinct strains were then screened for CLA production after 72 h of incubation with 0.5 mg of free linoleic acid ml−1 by using gas-liquid chromatography. The most efficient producers belonged to the species Bifidobacterium breve, of which two different strains converted 29 and 27% of the free linoleic acid to the cis-9, trans-11 isomer per microgram of dry cells, respectively. In addition, a strain of Bifidobacterium bifidum showed a conversion rate of 18%/μg dry cells. The ability of some Bifidobacterium strains to produce CLA could be another human health-promoting property linked to members of the genus, given that this metabolite has demonstrated anticarcinogenic activity in vitro and in vivo.

Oxidation of alpha 1-protease inhibitor: role of lipid peroxidation products

1989 ◽  
Vol 66 (5) ◽  
pp. 2211-2215 ◽  
Author(s):  
V. Mohsenin ◽  
J. L. Gee
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Protease Inhibitor ◽  
Alpha Tocopherol ◽  
Inhibitory Capacity ◽  
Buffer Control

Previously we demonstrated that in vivo exposure of humans to NO2 resulted in significant inactivation of alpha 1-protease inhibitor (alpha 1-PI) in the bronchoalveolar lavage fluid. However, alpha 1-PI retains its elastase inhibitory activity in vitro when exposed to 10 times the concentration of NO2 used in vivo. We suggested exogenous oxidants such as O2 and NO2 exert their effect in vivo in part through lipid peroxidation. We investigated the mechanism of inactivation of alpha 1-PI in the presence or absence of lipids under oxidant atmosphere. alpha 1-PI in solutions containing phosphate buffer (control), 0.1 mM stearic acid (saturated fatty acid, 18:0), or 0.1 mM linoleic acid (polyunsaturated fatty acid, 18:2) was exposed to either N2 or NO2 (50 ppm for 4 h). Elastase inhibitory capacity of alpha 1-PI was significantly diminished in the presence of 0.1 mM linoleic acid and under NO2 atmosphere (75 +/- 8% of control, P less than 0.01), whereas there was no change in elastase inhibitory capacity of alpha 1-PI in the presence or absence (buffer only) of 0.1 mM stearic acid under a similar condition (109 +/- 11 and 94 +/- 6%, respectively). The inactivated alpha 1-PI as the result of peroxidized lipid could be reactivated by dithiothreitol and methionine sulfoxide peptide reductase, suggesting oxidation of methionine residue at the elastase inhibitory site. Furthermore the inhibitory effect of peroxidized lipid on alpha 1-PI could be prevented by glutathione and glutathione peroxidase and to some extent by alpha-tocopherol.

scholarly journals Human health effects of conjugated linoleic acid from milk and supplements

2011 ◽  
Vol 24 (2) ◽  
pp. 206-227 ◽  
Author(s):  
Tracy A. McCrorie ◽  
Edel M. Keaveney ◽  
Julie M. W. Wallace ◽  
Nino Binns ◽  
M. Barbara E. Livingstone
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Human Health ◽  
Body Fat ◽  
Intervention Studies ◽  
Human Subjects ◽  
Scientific Evidence ◽  
Health Benefits ◽  
Health Claims

The primary purpose of the present review was to determine if the scientific evidence available for potential human health benefits of conjugated linoleic acid (CLA) is sufficient to support health claims on foods based on milk naturally enriched with cis-9, trans-11-CLA (c9, t11-CLA). A search of the scientific literature was conducted and showed that almost all the promising research results that have emerged in relation to cancer, heart health, obesity, diabetes and bone health have been in animal models or in vitro. Most human intervention studies have utilised synthetic CLA supplements, usually a 50:50 blend of c9, t11-CLA and trans-10, cis-12-CLA (t10, c12-CLA). Of these studies, the only evidence that is broadly consistent is an effect on body fat and weight reduction. A previous review of the relevant studies found that 3.2 g CLA/d resulted in a modest body fat loss in human subjects of about 0.09 kg/week, but this effect was attributed to the t10, c12-CLA isomer. There is no evidence of a consistent benefit of c9, t11-CLA on any health conditions; and in fact both synthetic isomers, particularly t10, c12-CLA, have been suspected of having pro-diabetic effects in individuals who are already at risk of developing diabetes. Four published intervention studies using naturally enriched CLA products were identified; however, the results were inconclusive. This may be partly due to the differences in the concentration of CLA administered in animal and human studies. In conclusion, further substantiation of the scientific evidence relating to CLA and human health benefits are required before health claims can be confirmed.

Synergistic anti-tumor activity of paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel in vitro and in vivo

Biomaterials ◽  
2009 ◽  
Vol 30 (27) ◽  
pp. 4777-4785 ◽  
Author(s):  
Ding-Ding Guo ◽  
Cheng-Xiong Xu ◽  
Ji-Shan Quan ◽  
Chung-Kil Song ◽  
Hua Jin ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thermosensitive Hydrogel ◽  
Anti Tumor Activity

scholarly journals Effect of Conjugated Linoleic Acid Feeding on the Growth Performance and Meat Fatty Acid Profiles in Broiler: Meta-analysis

2013 ◽  
Vol 26 (7) ◽  
pp. 995-1002 ◽  
Author(s):  
Sangbuem Cho ◽  
Chaehwa Ryu ◽  
Jinho Yang ◽  
David Tinotenda Mbiriri ◽  
Chang-Weon Choi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Growth Performance ◽  
Conjugated Linoleic Acid ◽  
Meta Analysis ◽  
Fatty Acid Profiles

scholarly journals Effects of conjugated linoleic acid on proliferation and differentiation of bovine intramuscular preadipocyte in vitro

2021 ◽  
Author(s):  
Rong Wan ◽  
Qingxiang Meng ◽  
Zhou Zhenming ◽  
Wu hao
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Proliferation And Differentiation ◽  
Cell Proportion ◽  
Intramuscular Fat Content

ABSTRACTConjugated linoleic acid (CLA), a mixture of isomers of linoleic acid, has previously been shown to be able to increase intramuscular fat content in vivo and stimulate adipogenesis in intramuscular preadipocytes in vitro in pig. Unfortunately, there is little data to evaluate the effect of CLA on proliferation and differentiation of bovine intramuscular preadipocytes. This study investigated the regulation by CLA in proliferation and differentiation of bovine intramuscular preadipocytes. The results demonstrated that CLA significantly induced the expression of PPARγ and C/EBPα mRNA of bovine intramuscular preadipocytes as well as the accumulation of lipid in cultured intramuscular preadipocytes. Additionally, CLA significantly decreased the cell proportion of phase G0/G1, and remarkably increased the proportion of phase S+G2/M. Collectively, these results suggest that CLA promotes bovine intramuscular preadipocyte proliferation and differentiation.

scholarly journals 10t,12c-conjugated linoleic acid inhibits lipopolysaccharide-induced cyclooxygenase expression in vitro and in vivo

2005 ◽  
Vol 46 (10) ◽  
pp. 2134-2142 ◽  
Author(s):  
Guangming Li ◽  
David Barnes ◽  
Daniel Butz ◽  
Dale Bjorling ◽  
Mark E. Cook
Keyword(s):  
Linoleic Acid ◽  
Conjugated Linoleic Acid

scholarly journals Selenite and selenate affect the fatty acid profile in in vitro incubated ovine ruminal fluid containing linseed oil

2013 ◽  
Vol 58 (No. 7) ◽  
pp. 328-341 ◽  
Author(s):  
M. Czauderna ◽  
J. Kowalczyk ◽  
M. Marounek
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Profile ◽  
Linseed Oil ◽  
Ruminal Fluid ◽  
Cla Isomers ◽  
Control Fluid

The influence of selenite (Se<sup>IV</sup>) or selenate (Se<sup>VI</sup>) added to ovine ruminal fluid containing linseed oil (LO) on the profile of fatty acids (FA), particularly conjugated linoleic acid (CLA) isomers, was investigated. The ruminal fluid was incubated in vitro at 39&deg;C under CO<sub>2</sub> either alone (the control fluid) or with LO (3.3 mg/ml) or with a combination of LO with either a low (0.167 &mu;g/ml) or high (1.67 &mu;g/ml) level of Se as Se<sup>IV</sup> or Se<sup>VI</sup>. LO added to ruminal fluids also provides an extra source of energy. The tubes with the examined fluids were removed after 0, 6, 12, 18, or 24 h of in vitro incubation and then analyzed to determine the FA levels. The lower and higher concentration of Se<sup>IV </sup>in the fluids with the LO revealed negligible effect on the concentration of the sum of the CLA isomers (&sum;CLA) in the fluid compared with the fluid with LO alone. The addition of a higher amount of Se<sup>IV</sup> to the fluid containing LO usually decreased the concentration of &sum;CLA compared with the fluid containing the lower concentration of Se<sup>IV</sup> and LO. The concentration of c9t11c15C18:3 (cLNA) in the fluids with LO, irrespective of the presence of extra Se, increased throughout the incubations, although the addition of Se<sup>IV </sup>or Se<sup>VI</sup> to the fluids containing LO numerically reduced the increase of the concentration of cLNA compared with the fluid with LO alone. The concentration sum of the C18:1 isomers (&Sigma;C18:1) in the control fluid numerically decreased throughout the incubations, while LO added to the fluid increased the concentration of &Sigma;C18:1 throughout the incubations. LO added to the fluid, irrespective of the presence of Se<sup>IV</sup> or Se<sup>VI</sup>, significantly increased the concentration of &Sigma;C18:1 compared with the control fluid and the fluids with Se<sup>IV </sup>or Se<sup>VI</sup>. The concentrations of C16:0 and C18:0 in the control fluid and the fluids containing Se<sup>IV</sup> or Se<sup>VI </sup>numerically increased throughout the incubations and were usually lower than in the fluids containing LO without or with Se<sup>IV </sup>or Se<sup>VI</sup>. The concentration of C18:3n-3 decreased throughout the incubation of the fluids containing LO, irrespective of the presence of Se<sup>IV </sup>or Se<sup>VI</sup>. LO added to the fluids, irrespective of the presence of Se<sup>IV</sup> or Se<sup>VI</sup>, increased the concentration of C18:2n-6 compared with the control fluid and the fluids with Se<sup>IV </sup>or Se<sup>VI</sup>. The higher concentration of Se<sup>IV</sup> or Se<sup>VI</sup> in the fluid with LO most efficiently increased the concentration of c5c8c11c14c17C20:5 compared with the control fluid or the fluids containing LO, irrespective of the presence of the lower concentration of Se<sup>IV</sup> or Se<sup>VI</sup>. LO added to the fluid, irrespective of the presence of Se<sup>IV </sup>or Se<sup>VI</sup>, increased the concentration of polyunsaturated FA compared with the control fluid or the fluids containing Se<sup>IV </sup>or Se<sup>VI</sup>. &nbsp; &nbsp;

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