scholarly journals Bacterial diversity of chronic middle ear lesions revealed by high-throughput sequencing

2021 ◽  
Author(s):  
Xingzhi Gu ◽  
Xiuqing Cheng ◽  
Tuoheti Abulajiang ◽  
Xiaoban Huang
Keyword(s):  
Bacterial Diversity ◽  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Epithelial Tissue ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Middle Ear Cholesteatoma

AbstractChronic otitis media is a common middle ear disease in otolaryngology and head and neck surgery. Bacterial infection is considered the main cause of disease, but relying on conventional bacterial cultures can be problematic for identification of specific pathogens. Current research suggests that bacteria in microbial communities can only be identified by rDNA sequencing of bacteria.This prospective study utilized broad-range PCR amplification of 16S rRNA genes with clone analysis to compare bacterial diversity in lesions from 6 patients with chronic suppurative otitis media (CSOM) and 10 patients with cholesteatoma of middle ear lesions. Bacteria were analyzed at the phylum, order, family, genus, and species levels. Bacterial species abundance and species diversity were greater in cholesteatoma of the middle ear lesions than in CSOM lesions. At all bacterial taxonomic levels, the epithelial tissue of middle ear cholesteatoma was complex in terms of bacterial diversity, covering a large number of Gram-positive and Gram-negative bacteria, likely related to bacterial microbiome formation. In contrast, bacteriology of the CSOM lesions was relatively simple at all taxonomic levels, with all sequences characterized as belonging to Gram-negative bacteria. These results suggest that persistent infection of middle ear cholesteatoma may be a microbial flora disorder, which is related to conditional pathogenic bacteria, rather than a single bacterial infectious disease. Findings from the study may have implications in the selection of antimicrobial agents for the treatment of chronic otitis media.

scholarly journals Correlation between Presence of Viable Bacteria and Presence of Endotoxin in Middle-Ear Effusions

1998 ◽  
Vol 36 (11) ◽  
pp. 3417-3419 ◽  
Author(s):  
Jeffrey R. Dingman ◽  
Mark G. Rayner ◽  
Suman Mishra ◽  
Yingze Zhang ◽  
Miles D. Ehrlich ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Otitis Media ◽  
Middle Ear ◽  
Moraxella Catarrhalis ◽  
Pediatric Patients ◽  
Chronic Otitis Media ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Viable Bacteria

The presence of endotoxin (detected by the Limulusamebocyte lysate assay) was compared to the presence of viableHaemophilus influenzae and Moraxella catarrhalis (detected by PCR) in 106 middle-ear effusions from pediatric patients with chronic otitis media. Endotoxin was found in 81 of the 106 specimens. Of these 81 specimens, 66 (81.5%) also tested positive for one or both of the gram-negative bacteria H. influenzae and M. catarrhalis. The data suggest that viable gram-negative bacteria, detectable by PCR but often undetectable by culture, may be the source of endotoxin in middle-ear effusions.

scholarly journals Clinical analysis on surgery of middle ear cholesteatoma and chronic otitis media

2016 ◽  
Vol 130 (S3) ◽  
pp. S221-S221
Author(s):  
Ayiheng Qukuerhan ◽  
Nilipaer Alimu ◽  
Halimulati Muertiza ◽  
Pilidong Kuyaxi
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Clinical Analysis ◽  
Middle Ear Cholesteatoma

Histology, Pathogenesis, and Treatment of Cholesteatoma

1984 ◽  
Vol 93 (4_suppl) ◽  
pp. 125-128 ◽  
Author(s):  
Maxwell Abramson ◽  
Hiroshi Moriyama ◽  
Cheng Chun Huang
Keyword(s):  
Animal Model ◽  
Bone Resorption ◽  
Connective Tissue ◽  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Increase Bone Resorption ◽  
Pathogenic Factors ◽  
Middle Ear Cholesteatoma

Factors present in cholesteatoma connective tissue induce bone resorption in chronic otitis media. The presence of skin and its products appears to exacerbate the destructive influence of connective tissue. One exacerbating factor is pressure, shown in an animal model to increase bone resorption by influencing the subepithelial connective tissue. Consideration of pathogenic factors provides a rationale for modifications in the treatment of middle ear cholesteatoma.

Activation of NLRP3 inflammasome in human middle ear cholesteatoma and chronic otitis media

2015 ◽  
Vol 136 (2) ◽  
pp. 136-140 ◽  
Author(s):  
Shin Kariya ◽  
Mitsuhiro Okano ◽  
Pengfei Zhao ◽  
Yuko Kataoka ◽  
Junko Yoshinobu ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Nlrp3 Inflammasome ◽  
Chronic Otitis Media ◽  
Middle Ear Cholesteatoma ◽  
Human Middle Ear

scholarly journals Expression of matrix metalloproteinase-9 in acquired middle ear cholesteatoma

2020 ◽  
Vol 11 (1) ◽  
pp. 29-36
Author(s):  
Dalibor Vranješ ◽  
Slobodan Spremo ◽  
Siniša Šolaja
Keyword(s):  
Matrix Metalloproteinase ◽  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Matrix Metalloproteinase 9 ◽  
Expression Levels ◽  
Positive Expression ◽  
Middle Ear Cholesteatoma ◽  
Acquired Cholesteatoma ◽  
Metalloproteinase 9

Introduction. Matrix metalloproteinase-9 (MMP-9) plays one of the key roles in the migration of inflammatory cells and the destructive behavior of acquired middle ear cholesteatoma. The aim of the study was to determine the expression levels of MMP-9 in chronic otitis media, with and without acquired cholesteatoma, as well as to determine the correlation between them and the pathomorphological changes in the tympanic membrane (TM). Methods. Immunohistochemical study included 178 patients of both sexes, aged 5-75 years, who underwent microsurgical treatment of chronic otitis media (COM) at the Ear, Nose and Throat Department of the University Clinical Center of the Republic of Srpska in Banja Luka from 2015 to 2018. Patients were divided into two groups on the basis of the presence or absence of acquired cholesteatoma: 97 (CCOM) and 81 (COMWC). The samples of the perimatrix of acquired cholesteatoma (n=97) and inflamed middle ear mucosa (n=81) were taken intraoperatively. The presence of retraction and certain types and subtypes of TM perforations were examined by intraoperative exploration. MMP-9 expression levels were determined by immunohistochemical analysis. Results. In the presence of cholesteatoma and highly positive expression of MMP-9, the marginal type of TM perforation was more likely to occur (p<0.01) while in negative expression of MMP-9 in the COMWC group, the central type was more likely to occur (p<0.01). There was a higher probability of the presence of attic subtype of TM perforation in patients with cholesteatoma and moderate positive expression of MMP-9 (p<0.01). There was not a statistically significant contribution of any of the factors in the prediction of the influence on the occurrence of TM retraction. Conclusion. The presence of acquired middle ear cholesteatoma and highly positive expression of MMP-9 are statistically significant predictors of the presence of marginal type and attic subtype of TM perforation. Expression levels of MMP-9 may have potential clinical significance in the development of irreversible pathomorphological changes on TM in middle ear cholesteatoma.

Clinical Characteristics and Surgical Benefits and Problems of Chronic Otitis Media and Middle Ear Cholesteatoma in Elderly Patients Older Than 70 Years

2012 ◽  
Vol 33 (7) ◽  
pp. 1213-1217 ◽  
Author(s):  
Akihiro Shinnabe ◽  
Mariko Hara ◽  
Masayo Hasegawa ◽  
Shingo Matsuzawa ◽  
Hiromi Kanazawa ◽  
...  
Keyword(s):  
Elderly Patients ◽  
Otitis Media ◽  
Middle Ear ◽  
Clinical Characteristics ◽  
Chronic Otitis Media ◽  
Middle Ear Cholesteatoma

Comparison of the Aditus ad Antrum Diameter on CT between Patients with Chronic Otitis Media and Healthy Individuals

2019 ◽  
Vol 15 (10) ◽  
pp. 990-993 ◽  
Author(s):  
Elif Gündoğdu ◽  
Uğur Toprak
Keyword(s):  
Computed Tomography ◽  
Otitis Media ◽  
Middle Ear ◽  
Healthy Subjects ◽  
Normal Values ◽  
Chronic Otitis Media ◽  
Healthy Individuals ◽  
Inner Diameter ◽  
Male Patients ◽  
The Right

Background: The middle ear cavity is ventilated through the aditus ad antrum. Aditus blockage contributes to the pathology of otitis media. Objective: To determine the normal values of the aditus ad antrum diameter on computed tomography and to investigate its relationship with chronic otitis media and related pathologies (tympanosclerosis and myringosclerosis). Methods: The temporal CT images of 162 individuals were evaluated retrospectively. In the axial sections, the inner diameter of the aditus was measured at the narrowest point at the cortex. The differences in diameter were compared between diseased and healthy ears. Results: In healthy individuals, the diameter was narrower in women. There was no difference between the right and left ears in healthy subjects. No correlation was found between age and diameter. In male patients with myringosclerosis, the diameter was slightly narrower on both sides but more marked on the left. In female patients with myringosclerosis, the diameter in both ears was slightly narrower. In cases of otitis media and tympanosclerosis, the diameter was less than that of healthy individuals, despite the lack of statistically significant result in all cases. Conclusion: The aditus ad antrum was narrower in diseased ears, indicating that a blocked aditus may contribute to the development of otitis media, as well as mucosal diseases.

scholarly journals Consistent Metagenome-Derived Metrics Verify and Delineate Bacterial Species Boundaries

mSystems ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Matthew R. Olm ◽  
Alexander Crits-Christoph ◽  
Spencer Diamond ◽  
Adi Lavy ◽  
Paula B. Matheus Carnevali ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Ribosomal Proteins ◽  
Large Scale ◽  
Bacterial Species ◽  
Bacterial Genome ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Species Discrimination ◽  
Bacterial Genomes ◽  
Discrimination Power

ABSTRACT Longstanding questions relate to the existence of naturally distinct bacterial species and genetic approaches to distinguish them. Bacterial genomes in public databases form distinct groups, but these databases are subject to isolation and deposition biases. To avoid these biases, we compared 5,203 bacterial genomes from 1,457 environmental metagenomic samples to test for distinct clouds of diversity and evaluated metrics that could be used to define the species boundary. Bacterial genomes from the human gut, soil, and the ocean all exhibited gaps in whole-genome average nucleotide identities (ANI) near the previously suggested species threshold of 95% ANI. While genome-wide ratios of nonsynonymous and synonymous nucleotide differences (dN/dS) decrease until ANI values approach ∼98%, two methods for estimating homologous recombination approached zero at ∼95% ANI, supporting breakdown of recombination due to sequence divergence as a species-forming force. We evaluated 107 genome-based metrics for their ability to distinguish species when full genomes are not recovered. Full-length 16S rRNA genes were least useful, in part because they were underrecovered from metagenomes. However, many ribosomal proteins displayed both high metagenomic recoverability and species discrimination power. Taken together, our results verify the existence of sequence-discrete microbial species in metagenome-derived genomes and highlight the usefulness of ribosomal genes for gene-level species discrimination. IMPORTANCE There is controversy about whether bacterial diversity is clustered into distinct species groups or exists as a continuum. To address this issue, we analyzed bacterial genome databases and reports from several previous large-scale environment studies and identified clear discrete groups of species-level bacterial diversity in all cases. Genetic analysis further revealed that quasi-sexual reproduction via horizontal gene transfer is likely a key evolutionary force that maintains bacterial species integrity. We next benchmarked over 100 metrics to distinguish these bacterial species from each other and identified several genes encoding ribosomal proteins with high species discrimination power. Overall, the results from this study provide best practices for bacterial species delineation based on genome content and insight into the nature of bacterial species population genetics.

scholarly journals Tissue Concentrations of Enoxacin in the Middle Ear of Patients with Chronic Otitis Media.

1992 ◽  
Vol 85 (1) ◽  
pp. 131-135
Author(s):  
Nozomu Mori ◽  
Akio Shugyo ◽  
Hiroshi Furuta ◽  
Yasuki Watanabe ◽  
Takafumi Kawahara ◽  
...  
Keyword(s):  
Otitis Media ◽  
Middle Ear ◽  
Chronic Otitis Media ◽  
Tissue Concentrations
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