Rootstocks influence the response of ripening grape berries to leafroll associated viruses

Grapevine leafroll-associated virus (GLRaV) infections are accompanied by symptoms with varying severity. Using a dedicated experimental vineyard, we studied the responses to GLRaVs in ripening berries from Cabernet franc grapevines grafted to different rootstocks and with zero, one, or pairs of leafroll infection(s). RNA sequencing data were mapped to a high-quality Cabernet franc genome reference assembled to carry out this study and integrated with hormone and metabolite abundance data. This study identified several molecular levers that participate in responses to GLRaVs, including those that are condition-dependent. This included describing common responses to GLRaVs that were reproduced in two consecutive years, in plants grafted to different rootstocks, and in more than one infection condition. Though different infections were inconsistently distinguishable from one another overall, the effects of infections in plants grafted to different rootstocks were distinct at each developmental stage. Conserved responses included the modulation of pathogen detecting genes, increases in abscisic acid signaling and cytoskeleton remodeling gene expression. The abundance of abscisic acid (ABA), related metabolites, ABA and hormone signaling-related gene expression, and the expression of several transcription factor families differentiated rootstocks overall. These data show that rootstock influences the effect of GLRaVs in ripening berries.

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Immune-related gene expression of Apis mellifera larvae in response to cold stress and Abscisic Acid (ABA) dietary supplementation

Journal of Apicultural Research ◽

10.1080/00218839.2019.1708653 ◽

2020 ◽

Vol 59 (4) ◽

pp. 669-676 ◽

Cited By ~ 1

Author(s):

Pedro Negri ◽

Leonor Ramirez ◽

Silvina Quintana ◽

Nicolas Szawarski ◽

Matías D. Maggi ◽

...

Keyword(s):

Gene Expression ◽

Abscisic Acid ◽

Apis Mellifera ◽

Cold Stress ◽

Dietary Supplementation ◽

Related Gene ◽

Immune Related Gene

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Interleukin-6 actions in the hypothalamus protects against obesity and is involved in the regulation of neurogenesis

Journal of Neuroinflammation ◽

10.1186/s12974-021-02242-8 ◽

2021 ◽

Vol 18 (1) ◽

Author(s):

Vanessa C. Bobbo ◽

Daiane F. Engel ◽

Carlos Poblete Jara ◽

Natalia F. Mendes ◽

Roberta Haddad-Tovolli ◽

...

Keyword(s):

Gene Expression ◽

Interleukin 6 ◽

Ex Vivo ◽

Cell Types ◽

Cell Labeling ◽

Specific Cell ◽

Related Gene ◽

Sequencing Data ◽

Ex Vivo Model ◽

Diet Induced Obesity

Abstract Background Interleukin-6 (IL6) produced in the context of exercise acts in the hypothalamus reducing obesity-associated inflammation and restoring the control of food intake and energy expenditure. In the hippocampus, some of the beneficial actions of IL6 are attributed to its neurogenesis-inducing properties. However, in the hypothalamus, the putative neurogenic actions of IL6 have never been explored, and its potential to balance energy intake can be an approach to prevent or attenuate obesity. Methods Wild-type (WT) and IL6 knockout (KO) mice were employed to study the capacity of IL6 to induce neurogenesis. We used cell labeling with Bromodeoxyuridine (BrdU), immunofluorescence, and real-time PCR to determine the expression of markers of neurogenesis and neurotransmitters. We prepared hypothalamic neuroprogenitor cells from KO that were treated with IL6 in order to provide an ex vivo model to further characterizing the neurogenic actions of IL6 through differentiation assays. In addition, we analyzed single-cell RNA sequencing data and determined the expression of IL6 and IL6 receptor in specific cell types of the murine hypothalamus. Results IL6 expression in the hypothalamus is low and restricted to microglia and tanycytes, whereas IL6 receptor is expressed in microglia, ependymocytes, endothelial cells, and astrocytes. Exogenous IL6 reduces diet-induced obesity. In outbred mice, obesity-resistance is accompanied by increased expression of IL6 in the hypothalamus. IL6 induces neurogenesis-related gene expression in the hypothalamus and in neuroprogenitor cells, both from WT as well as from KO mice. Conclusion IL6 induces neurogenesis-related gene expression in the hypothalamus of WT mice. In KO mice, the neurogenic actions of IL6 are preserved; however, the appearance of new fully differentiated proopiomelanocortin (POMC) and neuropeptide Y (NPY) neurons is either delayed or disturbed.

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Changes in the abscisic acid levels and related gene expression during fruit development and ripening in bilberry (Vaccinium myrtillus L.)

Phytochemistry ◽

10.1016/j.phytochem.2013.06.023 ◽

2013 ◽

Vol 95 ◽

pp. 127-134 ◽

Cited By ~ 34

Author(s):

Katja Karppinen ◽

Elina Hirvelä ◽

Tiina Nevala ◽

Nina Sipari ◽

Marko Suokas ◽

...

Keyword(s):

Gene Expression ◽

Abscisic Acid ◽

Fruit Development ◽

Vaccinium Myrtillus ◽

Related Gene ◽

Fruit Development And Ripening ◽

Vaccinium Myrtillus L

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Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

Genetics and Molecular Research ◽

10.4238/2012.september.10.5 ◽

2012 ◽

Vol 11 (4) ◽

pp. 4063-4080 ◽

Cited By ~ 60

Author(s):

W.L. Guo ◽

R.G. Chen ◽

Z.H. Gong ◽

Y.X. Yin ◽

S.S. Ahmed ◽

...

Keyword(s):

Gene Expression ◽

Antioxidant Enzymes ◽

Abscisic Acid ◽

Capsicum Annuum ◽

Chilling Stress ◽

Related Gene

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Ethephon-Induced Ethylene Enhances Starch Degradation and Sucrose Transport with an Interactive Abscisic Acid-Mediated Manner in Mature Leaves of Oilseed rape (Brassica napus L.)

Plants ◽

10.3390/plants10081670 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1670

Author(s):

Bok-Rye Lee ◽

Rashed Zaman ◽

Van Hien La ◽

Dong-Won Bae ◽

Tae-Hwan Kim

Keyword(s):

Gene Expression ◽

Abscisic Acid ◽

Leaf Senescence ◽

Starch Content ◽

Starch Degradation ◽

Bhlh Transcription Factor ◽

Related Gene ◽

Ethylene Concentration ◽

Mature Leaves ◽

Enhanced Expression

The leaf senescence process is characterized by the degradation of macromolecules in mature leaves and the remobilization of degradation products via phloem transport. The phytohormone ethylene mediates leaf senescence. This study aimed to investigate the ethephon-induced ethylene effects on starch degradation and sucrose remobilization through their interactive regulation with other hormones. Ethephon (2-chloroethylphosphonic acid) was used as an ethylene-generating agent. Endogenous hormonal status, carbohydrate compounds, starch degradation-related gene expression, sucrose transporter gene expression, and phloem sucrose loading were compared between the ethephon-treated plants and controls. Foliar ethephon spray enhanced the endogenous ethylene concentration and accelerated leaf senescence, as evidenced by reduced chlorophyll content and enhanced expression of the senescence-related gene SAG12. Ethephon-enhanced ethylene prominently enhanced the endogenous abscisic acid (ABA) level. accompanied with upregulation of ABA synthesis gene 9-cis-epoxycarotenoid dioxygenase (NCED3), ABA receptor gene pyrabactin resistance 1 (PYR1), and ABA signaling genes sucrose non-fermenting 1 (Snf1)-related protein kinase 2 (SnRK2), ABA-responsive element binding 2 (AREB2), and basic-helix-loop-helix (bHLH) transcription factor (MYC2).) Ethephon treatment decreased starch content by enhancing expression of the starch degradation-related genes α-amylase 3 (AMY3) and β-amylase 1 (BAM1), resulting in an increase in sucrose content in phloem exudates with enhanced expression of sucrose transporters, SUT1, SUT4, and SWEET11. These results suggest that a synergistic interaction between ethylene and ABA might account for sucrose accumulation, mainly due to starch degradation in mature leaves and sucrose phloem loading in the ethephon-induced senescent leaves.

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