Ubiquity of the Symbiont Serratia symbiotica in the Aphid Natural Environment: Distribution, Diversity and Evolution at a Multitrophic Level

ABSTRACTBacterial symbioses are significant drivers of insect evolutionary ecology. However, despite recent findings that these associations can emerge from environmentally derived bacterial precursors, there is still little information on how these potential progenitors of insect symbionts circulates in the trophic systems. The aphid symbiont Serratia symbiotica represents a valuable model for deciphering evolutionary scenarios of bacterial acquisition by insects, as its diversity includes intracellular host-dependent strains as well as gut-associated strains that have retained some ability to live independently of their hosts and circulate in plant phloem sap. These strains represent a potential reservoir for the emergence of new and more intimate symbioses. Here, we conducted a field study to examine the distribution and diversity of S. symbiotica found in aphid populations, as well as in different compartments of their surrounding environment. A total of 250 aphid colonies, 203 associated insects, and 161 plant samples associated with aphid colonies were screened for S. symbiotica. Twenty percent of aphids were infected with S. symbiotica, and the symbiont includes a wide diversity of strains with varied tissue tropism corresponding to different lifestyle. We also showed that the prevalence of S. symbiotica is influenced by seasonal temperatures. For the first time, we found that S. symbiotica was present in non aphid species and in host plants, and that the prevalence of the bacterium in these samples was higher when associated aphid colonies were infected. Furthermore, phylogenetic analyses suggest the existence of horizontal transfers between the different trophic levels examined. These results provide a completely new picture of the ubiquity of an insect symbiont in nature. They suggest that ecological interactions promote the dissemination of strains that are still free-living and poorly specialized, and for which plants are a proabable reservoir for the acquisition of new bacterial partners in insects.

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Buchnera has changed flatmate but the repeated replacement of co-obligate symbionts is not associated with the ecological expansions of their aphid hosts

10.1101/086223 ◽

2016 ◽

Author(s):

A.S. Meseguer ◽

A. Manzano-Marín ◽

A. Coeur d’Acier ◽

A-L. Clamens ◽

M. Godefroid ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Aphid Species ◽

Buchnera Aphidicola ◽

Evolutionary Transitions ◽

Symbiotic Associations ◽

Metabolic Functions ◽

History Of ◽

Serratia Symbiotica ◽

Bacterial Genes

AbstractSymbiotic associations with bacteria have facilitated important evolutionary transitions in insects and resulted in long-term obligate interactions. Recent evidence suggests that these associations are not always evolutionarily stable and that symbiont replacement and/or supplementation of an obligate symbiosis by an additional bacterium has occurred during the history of many insect groups. Yet, the factors favoring one symbiont over another in this evolutionary dynamic are not well understood; progress has been hindered by our incomplete understanding of the distribution of symbionts across phylogenetic and ecological contexts. While many aphids are engaged into an obligate symbiosis with a single Gammaproteobacterium, Buchnera aphidicola, in species of the Lachninae subfamily, this relationship has evolved into a “ménage à trois”, in which Buchnera is complemented by a cosymbiont, usually Serratia symbiotica. Using deep sequencing of 16S rRNA bacterial genes from 128 species of Cinara (the most diverse Lachninae genus), we reveal a highly dynamic dual symbiotic system in this aphid lineage. Most species host both Serratia and Buchnera but, in several clades, endosymbionts related to Sodalis, Erwinia or an unnamed member of the Enterobacteriaceae have replaced Serratia. Endosymbiont genome sequences from four aphid species+confirm that these coresident symbionts fulfill essential metabolic functions not ensured by Buchnera. We further demonstrate through comparative phylogenetic analyses that co-symbiont replacement is not associated with the adaptation of aphids to new ecological conditions. We propose that symbiont succession was driven by factors intrinsic to the phenomenon of endosymbiosis, such as rapid genome deterioration or competitive interactions between bacteria with similar metabolic capabilities.

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Towards a Natural Classification of Hyphodontia Sensu Lato and the Trait Evolution of Basidiocarps within Hymenochaetales (Basidiomycota)

Journal of Fungi ◽

10.3390/jof7060478 ◽

2021 ◽

Vol 7 (6) ◽

pp. 478

Author(s):

Xue-Wei Wang ◽

Tom W. May ◽

Shi-Liang Liu ◽

Li-Wei Zhou

Keyword(s):

Phylogenetic Analyses ◽

Taxonomic Status ◽

Ancestral State ◽

Trait Evolution ◽

Natural Classification ◽

Multiple Loci ◽

The Family ◽

Phylogenetic Perspective ◽

Taxonomic Framework ◽

First Time

Hyphodontia sensu lato, belonging to Hymenochaetales, accommodates corticioid wood-inhabiting basidiomycetous fungi with resupinate basidiocarps and diverse hymenophoral characters. Species diversity of Hyphodontia sensu lato has been extensively explored worldwide, but in previous studies the six accepted genera in Hyphodontia sensu lato, viz. Fasciodontia, Hastodontia, Hyphodontia, Kneiffiella, Lyomyces and Xylodon were not all strongly supported from a phylogenetic perspective. Moreover, the relationships among these six genera in Hyphodontia sensu lato and other lineages within Hymenochaetales are not clear. In this study, we performed comprehensive phylogenetic analyses on the basis of multiple loci. For the first time, the independence of each of the six genera receives strong phylogenetic support. The six genera are separated in four clades within Hymenochaetales: Fasciodontia, Lyomyces and Xylodon are accepted as members of a previously known family Schizoporaceae, Kneiffiella and Hyphodontia are, respectively, placed in two monotypic families, viz. a previous name Chaetoporellaceae and a newly introduced name Hyphodontiaceae, and Hastodontia is considered to be a genus with an uncertain taxonomic position at the family rank within Hymenochaetales. The three families emerged between 61.51 and 195.87 million years ago. Compared to other families in the Hymenochaetales, these ages are more or less similar to those of Coltriciaceae, Hymenochaetaceae and Oxyporaceae, but much older than those of the two families Neoantrodiellaceae and Nigrofomitaceae. In regard to species, two, one, three and 10 species are newly described from Hyphodontia, Kneiffiella, Lyomyces and Xylodon, respectively. The taxonomic status of additional 30 species names from these four genera is briefly discussed; an epitype is designated for X. australis. The resupinate habit and poroid hymenophoral configuration were evaluated as the ancestral state of basidiocarps within Hymenochaetales. The resupinate habit mainly remains, while the hymenophoral configuration mainly evolves to the grandinioid-odontioid state and also back to the poroid state at the family level. Generally, a taxonomic framework for Hymenochaetales with an emphasis on members belonging to Hyphodontia sensu lato is constructed, and trait evolution of basidiocarps within Hymenochaetales is revealed accordingly.

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Clathrosporium retortum, a novel aeroaquatic fungus in the Sordariomycetidae (Ascomycota) from Brazil

Phytotaxa ◽

10.11646/phytotaxa.239.1.2 ◽

2015 ◽

Vol 239 (1) ◽

pp. 17

Author(s):

Larissa Bernardino Moro ◽

Gregorio Delgado ◽

Iracema Helena SCHOENLEIN-CRUSIUS

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Type Specimen ◽

Molecular Data ◽

Sao Paulo ◽

São Paulo ◽

Present Fungus ◽

Broad Concept ◽

Morphological And Molecular Data ◽

First Time

Clathrosporium retortum sp. nov., collected on submerged mixed leaf litter samples at Ilha do Cardoso State Park, São Paulo state, Brazil, is described based on morphological and molecular data. The fungus is characterized by forming whitish, dense, subglobose to irregular propagules, hyaline to subhyaline when young, subhyaline to dark brown at maturity, that are formed by densely interwoven conidial filaments with each conidial cell repeatedly branching bilaterally or occasionally unilaterally. Phylogenetic analyses using partial LSU nrDNA sequence data suggest that C. retortum belongs in the Sordariomycetes (Ascomycota) where it forms a well-supported clade with Clohesia corticola in the Sordariomycetidae, but its ordinal or familial placement remains unresolved. Its phylogenetic placement confirms the polyphyletic nature of aeroaquatic fungi like Clathrosporium, as it was distantly related to one available sequence in GenBank named as C. intricatum, the type species, which is phylogenetically related to the Helotiales (Leotiomycetes). However, due to lack of authenticity of the identity of this sequence with the type specimen of C. intricatum, a broad concept of Clathrosporium is tentatively adopted here to accommodate the present fungus instead of introducing a new genus. Beverwykella clathrata, Helicoön septatissimum and Peyronelina glomerulata are recorded for the first time from Brazil. Cancellidium applanatum and Candelabrum brocchiatum are new records for the state of São Paulo.

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Aphid species and morphs new to the fauna of Finland (Homoptera, Aphididae, Macrosiphini)

Entomologica Fennica ◽

10.33338/ef.83359 ◽

1990 ◽

Vol 1 (2) ◽

pp. 65-98

Author(s):

Osmo Heikinheimo

Keyword(s):

Diagnostic Value ◽

Aphid Species ◽

Junior Synonym ◽

New Subgenus ◽

First Time

Collecting data are represented on 26 species and subspecies new to Finland and some morphs are described for the first time. Comments on some species are given and new characters of diagnostic value are described for several species. Acyrthosiphon aurlandicum Heikinheimo, 1966 is a junior synonym of A. brachysiphon Hille Ris Lambers, 1952. A new subgenus, Metobion, with typus subgen. Acyrthosiphon (Metopolophium) graminearum Mordvilko, 1919, is erected in the genus Sitobion Mordvilko, 1914.

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Identification the Presence of Clade 2.3.2.1c-Avian Influenza H5N1, a Highly Pathogenic Virus in Iraq, 2018

Macedonian Veterinary Review ◽

10.2478/macvetrev-2019-0024 ◽

2019 ◽

Vol 42 (2) ◽

pp. 189-194

Author(s):

Furkan Alaraji ◽

Hussam Muhsen ◽

Abdullah O. Alhatami ◽

Yahia Ismail Khudhair

Keyword(s):

Avian Influenza ◽

Serological Test ◽

Phylogenetic Analyses ◽

Highly Pathogenic ◽

Glycoprotein Gene ◽

Pathogenic Virus ◽

Hpai H5n1 ◽

First Time ◽

Clade 2.3.2.1C ◽

Influenza H5n1

Abstract For the first time in Iraq, we identified in March, 2018 the presence of a highly virulent avian influenza virus (AIV), H5N1 (Clade 2.3.2.1c), causing highly pathogenic avian influenza (HPAI) in poultry farms, Iraq,. The identification of the virus was done using a rapid serological test, a real time-qPCR, and glycoprotein gene sequencing. Using sequencing and phylogenetic analyses, the clade 2.3.2.1c virus was recorded to be clustered, with high similarity to Asian and West African AIV, HPAI H5N1 from Ivory Coast identified in 2015. According to our knowledge, there was no previous detection of the clade 2.3.2.1c made in Iraq. Our results provide evidence that high risk of HPAI H5 outbreaks might be present in Iraq, and this needs to lead to high quality surveillance targeting of wild and domestic birds for early diagnosis of HPAI. The current work provides feasible and accurate approaches for understanding the evolution of HPAI H5 virus in different countries around the world.

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Delimitation, new species and teleomorph-anamorph relationships in Codinaea, Dendrophoma, Paragaeumannomyces and Striatosphaeria (Chaetosphaeriaceae)

MycoKeys ◽

10.3897/mycokeys.74.57824 ◽

2020 ◽

Vol 74 ◽

pp. 17-74

Author(s):

Martina Réblová ◽

Jana Nekvindová ◽

Jacques Fournier ◽

Andrew N. Miller

Keyword(s):

New Species ◽

Type Species ◽

Morphological Traits ◽

Phylogenetic Analyses ◽

Axenic Culture ◽

Monophyletic Clade ◽

Morphological Comparison ◽

The Family ◽

Terrestrial Habitats ◽

First Time

The Chaetosphaeriaceae are a diverse group of pigmented, predominantly phialidic hyphomycetes comprised of several holomorphic genera including Chaetosphaeria, the most prominent genus of the family. Although the morphology of the teleomorphs of the majority of Chaetosphaeria is rather uniform, their associated anamorphs primarily exhibit the variability and evolutionary change observed in the genus. An exception from the morphological monotony among Chaetosphaeria species is a group characterised by scolecosporous, hyaline to light pink, multiseptate, asymmetrical ascospores and a unique three-layered ascomatal wall. Paragaeumannomyces sphaerocellularis, the type species of the genus, exhibits these morphological traits and is compared with similar Chaetosphaeria with craspedodidymum- and chloridium-like synanamorphs. Morphological comparison and phylogenetic analyses of the combined ITS-28S sequences of 35 isolates and vouchers with these characteristics revealed a strongly-supported, morphologically well-delimited clade in the Chaetosphaeriaceae containing 16 species. The generic name Paragaeumannomyces is applied to this monophyletic clade; eight new combinations and five new species, i.e. P. abietinussp. nov., P. eleganssp. nov., P. granulatussp. nov., P. sabinianussp. nov. and P. smokiensissp. nov., are proposed. A key to Paragaeumannomyces is provided. Using morphology, cultivation studies and phylogenetic analyses of ITS and 28S rDNA, two additional new species from freshwater and terrestrial habitats, Codinaea paniculatasp. nov. and Striatosphaeria castaneasp. nov., are described in the family. A codinaea-like anamorph of S. castanea forms conidia with setulae at each end in axenic culture; this feature expands the known morphology of Striatosphaeria. A chaetosphaeria-like teleomorph is experimentally linked to Dendrophoma cytisporoides, a sporodochial hyphomycete and type species of Dendrophoma, for the first time.

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Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences

Nematology ◽

10.1163/156854102765216731 ◽

2003 ◽

Vol 5 (1) ◽

pp. 99-111 ◽

Cited By ~ 112

Author(s):

Zahra Tanha Maafi ◽

Sergei Subbotin ◽

Maurice Moens

Keyword(s):

Phylogenetic Analyses ◽

Species Level ◽

Its Rdna ◽

Heterodera Avenae ◽

Published Data ◽

Rdna Sequences ◽

First Time ◽

Group Species ◽

High Support ◽

Different Parts

Abstract RFLP and sequences of ITS-rDNA of 45 populations of cyst-forming nematodes collected from different parts of Iran were analysed and identified as representatives of 21 species. Eight enzymes generated RFLP for all studied populations. Comparison of RFLP profiles and sequences of the ITS regions with published data confirmed the presence of Heterodera avenae, H. filipjevi, H. glycines, H. hordecalis, H. latipons, H. schachtii and H. trifolii in Iran. RFLP patterns and ITS sequences for H. elachista, H. turcomanica, H. mothi and C. cacti were obtained for the first time in this study. Heterodera humuli, H. goettingiana, H. fici, H. elachista, H. turcomanica and Cactodera cacti are recorded for the first time in Iran. These results correspond with morphological and morphometric identification of the populations. Several populations were not identified at the species level and are attributed to Heterodera sp.; some of these may correspond to new species. Twenty-one new sequences from Iranian cyst-forming nematodes and 36 known sequences were used for the phylogenetic analyses. The cyst-forming nematodes formed several clades corresponding to their morphological features. Heterodera mothi and H. elachista clustered with high support with other Cyperi group species and H. turcomanica formed a moderately to highly supported clade with the Humuli group.

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Morphological and molecular characterization of two species of Gelidium (Gelidiales, Rhodophyta) from Mexico: G. nayaritense sp. nov. and the new record of G. sanyaense

Phytotaxa ◽

10.11646/phytotaxa.527.3.1 ◽

2021 ◽

Vol 527 (3) ◽

pp. 161-176

Author(s):

NATALY QUIROZ-GONZÁLEZ ◽

MA. EDITH PONCE-MÁRQUEZ ◽

NORMA LÓPEZ-GÓMEZ ◽

DENÍ RODRÍGUEZ

Keyword(s):

Gulf Of California ◽

Phylogenetic Analyses ◽

Distinct Species ◽

Tropical Region ◽

Mexican Pacific ◽

Molecular Approaches ◽

Diagnostic Characteristics ◽

Morphological Differences ◽

First Time

Gelidium pusillum is a species reported from the northern Gulf of California to the tropical region of the Mexican Pacific, but there is wide morphological variation among populations. The objective of this research was to evaluate of the Mexican species using morphological and molecular approaches, with the markers rbcL and COI-5P. This study examined 12 samples from four sites in the Mexican tropical Pacific. Phylogenetic analyses resolved these specimens within two clades separated from the topotype material of G. pusillum. The main morphological differences with other Mexican and phylogenetic closely related species were size, branching pattern, branching order, distribution of internal rhizoidal filaments, and shape of the tetrasporangial sori. The sequence divergences between these clades and the morphological differences support their representing distinct species so that Gelidium nayaritense sp. nov. is proposed for tropical specimens of the Mexican Pacific, whose diagnostic characteristics are the little or absent branching, its small size, as well as the presence of internal rhizoidal filaments concentrated in the subcortex, further G. sanyaense is register for first time from Mexico and Eastern Pacific.

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Aquatic organism behaviours at multiple trophic levels for water monitoring: the case of Gran Sasso - Sirente aquifer

10.5194/egusphere-egu21-15955 ◽

2021 ◽

Author(s):

Federica Di Giacinto ◽

Miriam Berti ◽

Luigi Carbone ◽

Riccardo Caprioli ◽

Valentina Colaiuda ◽

...

Keyword(s):

Irrigation Water ◽

Ecosystem Health ◽

Emerging Contaminants ◽

Aquatic Organism ◽

Early Warning Systems ◽

Trophic Levels ◽

Warning Systems ◽

First Time ◽

E Mail ◽

Early Warnings

Emerging contaminants could exert combined toxic effects, including synergetic and antagonistic ones, that cannot be identified by chemical analysis tools. The biological early warning systems (BEWS) perform a real-time and continuous (24 h) monitoring of physiological and/or behavioural parameters of organism alterations, potentially correlated to water pollution. They are based on the response of living sentinels (i.e. molluscs, algae, crustaceans, fish) to a contaminant or mixture of them. Early warnings can be sent by SMS, e-mail, etc. to operators, in order to activate response actions. Belonging to different trophic levels, the crustacean D. magna, the alga C. vulgaris and the mollusc P. casertanum have been used to control Gran Sasso-Sirente aquifer in three different locations. Drinkable water of Teramo province and irrigation water of L&#8217;Aquila have been continuously monitored by the commercial tools &#8220;bbe&#174; Daphnia Toximeter (DTOX)&#8221; and &#8220;bbe&#174; Algae Toximeter (ATOX)&#8221;, respectively. In Tirino river spring, a novel sensor &#8220;SmartShell&#8221; has registered for the first time the valve movements of the autochthonous bivalve. After the first testing period, DTOX and ATOX did not register any typology of alarms in the potable and irrigation water. The valve movements of P. casertanum have been examined through spectral analysis in order to evaluate the behavioural rhythms useful for further investigation on their alterations as early warnings. The objective has been to reinforce the aquifer protection by installing instruments internationally recognised as efficient tools and exploring new proposals for guaranteeing human and ecosystem health.

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How Does Nutrition Structure Ecosystems?

The Nature of Nutrition ◽

10.23943/princeton/9780691145655.003.0008 ◽

2012 ◽

Author(s):

Stephen J. Simpson ◽

David Raubenheimer

Keyword(s):

Evolutionary Ecology ◽

Ecosystem Dynamics ◽

Trophic Levels ◽

Nutritional Requirements ◽

Ecological Communities ◽

Geometric Framework ◽

Agent Based ◽

Fundamental Niche ◽

Biophysical Ecology ◽

Ecology Of Nutrition

This chapter assesses the consequences of individual nutrition for populations and the assemblages of species that comprise ecological communities. However, the ecological consequences of nutrition are not restricted to the effects of diet on individual organisms but include as well the direct and indirect interactions occurring among individuals within populations and between species. Understanding the complex network of interactions that produce food webs and structure ecosystem dynamics requires the understanding of the participants' differing nutritional requirements, priorities, and regulatory capacities. Geometric Framework analyses have shown that these features differ between species and across trophic levels. Nutritional space is one part of the fundamental niche of an organism, and there is a need to integrate nutrition with the biophysical ecology of organisms. Evolutionary processes also need to be taken into account, and agent-based models offer promise toward development of a new understanding of the evolutionary ecology of nutrition.

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