Cyclin-G-associated kinase GAK/Aux orchestrates glial autophagy via Atg9 phosphorylation in Parkinson's disease
Glia serve as double-edged swords to modulate neuropathology in Parkinson's disease (PD), but how they react opposingly to be beneficial or detrimental under pathological conditions, like promote or eliminate α-Synuclein inclusions, remain elusive. Here we present evidence that the PD risk factor Cyclin G-associated kinase (GAK)/dAuxilin (Aux) is a new component in glial autophagy. Lack of GAK/Aux promotes autophagic induction, disrupts lysosome acidification, and blocks glial clearance of substrates. Aux regulates Atg9 trafficking to autophagosomes and lysosomes for autophagosome biogenesis and autolysosome maturation, respectively, via Atg9 phosphorylation at newly identified residues T62 and T69. GAK/Aux-mediated glial autophagy is required for α-Syn degradation and contributes to a broad spectrum of parkinsonian symptoms in Drosophila and mice. Our findings indicate that glial autophagy is a critical component in PD progression and identify a new autophagy factor functioning in a tissue-specific manner; targeting glial autophagy is potentially a therapeutic solution for PD.