scholarly journals Impact of Human Serum Proteins on Susceptibility of Acinetobacter baumannii to Cefiderocol: role of iron transport

2021 ◽  
Author(s):  
Casin Le ◽  
Camila Pimentel ◽  
Fernando Pasteran ◽  
Marisel Romina Tuttobene ◽  
Tomas Subils ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Human Serum ◽  
Acinetobacter Baumannii ◽  
Iron Uptake ◽  
Iron Transport ◽  
Serum Proteins ◽  
Cell Entry ◽  
Altered Expression ◽  
Human Serum Proteins

Cefiderocol is a siderophore antibiotic that co-opts iron transporters to facilitate cell entry. We show that genes related to iron uptake systems and resistance to β-lactams in Acinetobacter baumannii have altered expression levels in the presence of human serum, human serum albumin, or human pleural fluid. Cefiderocol MICs are also raised in the presence of the mentioned fluids. Clinical response in A. baumannii infections may be related to the interplay of these human factors.

scholarly journals Tuning the interactions of decavanadate with thaumatin, lysozyme, proteinase K and human serum proteins by its coordination to a pentaaquacobalt(ii) complex cation

2019 ◽  
Vol 43 (45) ◽  
pp. 17863-17871 ◽  
Author(s):  
Lukáš Krivosudský ◽  
Alexander Roller ◽  
Annette Rompel
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Serum Proteins ◽  
Complex Cation ◽  
Proteinase K ◽  
Human Serum Proteins

Inorganic functionalization of the decavanadate anion promotes a different type of interaction with model proteins thaumatin, lysozyme, proteinase K, human serum albumin and transferrin.

Dimeric binding of plant alkaloid ellipticine to human serum proteins

RSC Advances ◽  
2016 ◽  
Vol 6 (50) ◽  
pp. 44096-44105 ◽  
Author(s):  
Ferenc Zsila ◽  
Tamás Beke-Somfai
Keyword(s):  
Circular Dichroism ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Serum Proteins ◽  
Glycoprotein P ◽  
Acid Glycoprotein ◽  
Circular Dichroïsm ◽  
Human Serum Proteins

Induced exciton circular dichroism signals reveal the accommodation of a pair of ellipticine molecules to the subdomain IB of human serum albumin and the β-barrel of α1-acid glycoprotein.

TESTOSTERONE BINDING BY HUMAN SERUM PROTEINS

1971 ◽  
Vol 68 (1_Supplb) ◽  
pp. S105 ◽  
Author(s):  
O. A. Lea ◽  
K. F. Støa
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Human Serum Proteins

scholarly journals Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the Host Determines the Outcome?

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 833
Author(s):  
Camila Pimentel ◽  
Casin Le ◽  
Marisel R. Tuttobene ◽  
Tomas Subils ◽  
Krisztina M. Papp-Wallace ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Human Serum Albumin ◽  
Quorum Sensing ◽  
Serum Albumin ◽  
Human Serum ◽  
Acinetobacter Baumannii ◽  
Biofilm Formation ◽  
Model Systems ◽  
Expression Levels ◽  
Multiple Drugs

Acinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenem-resistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their response with respect to antimicrobial resistance, biofilm formation, and quorum sensing, all features responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS depending on the strain. Expression levels of pbp1 and pbp3 tended to be increased by the presence of HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A. baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.

scholarly journals THE CYSTINE CONTENT OF HUMAN SERUM PROTEINS

1933 ◽  
Vol 100 (3) ◽  
pp. 775-778 ◽  
Author(s):  
L.R. Tuchman ◽  
Miriam Reiner
Keyword(s):  
Human Serum ◽  
Serum Proteins ◽  
Cystine Content ◽  
Human Serum Proteins

scholarly journals Human Pleural Fluid and Human Serum Albumin Modulate the Behavior of a Hypervirulent and Multidrug-Resistant (MDR) Acinetobacter baumannii Representative Strain

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 471
Author(s):  
Camila Pimentel ◽  
Casin Le ◽  
Marisel R. Tuttobene ◽  
Tomas Subils ◽  
Jasmine Martinez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Acinetobacter Baumannii ◽  
Pleural Fluid ◽  
Gene Expression Profile ◽  
Expression Profile ◽  
The United States ◽  
World Health

Acinetobacter baumannii is a nosocomial pathogen capable of causing serious infections associated with high rates of morbidity and mortality. Due to its antimicrobial drug resistance profile, A. baumannii is categorized as an urgent priority pathogen by the Centers for Disease Control and Prevention in the United States and a priority group 1 critical microorganism by the World Health Organization. Understanding how A. baumannii adapts to different host environments may provide critical insights into strategically targeting this pathogen with novel antimicrobial and biological therapeutics. Exposure to human fluids was previously shown to alter the gene expression profile of a highly drug-susceptible A. baumannii strain A118 leading to persistence and survival of this pathogen. Herein, we explore the impact of human pleural fluid (HPF) and human serum albumin (HSA) on the gene expression profile of a highly multi-drug-resistant strain of A. baumannii AB5075. Differential expression was observed for ~30 genes, whose products are involved in quorum sensing, quorum quenching, iron acquisition, fatty acid metabolism, biofilm formation, secretion systems, and type IV pilus formation. Phenotypic and further transcriptomic analysis using quantitative RT-PCR confirmed RNA-seq data and demonstrated a distinctive role of HSA as the molecule involved in A. baumannii’s response.

scholarly journals Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants

Biofouling ◽  
2016 ◽  
Vol 33 (1) ◽  
pp. 98-111 ◽  
Author(s):  
Francisco Romero-Gavilán ◽  
N. C. Gomes ◽  
Joaquin Ródenas ◽  
Ana Sánchez ◽  
Mikel Azkargorta ◽  
...  
Keyword(s):  
Human Serum ◽  
Dental Implants ◽  
Serum Proteins ◽  
Proteome Analysis ◽  
Titanium Surfaces ◽  
Human Serum Proteins
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