scholarly journals An evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature

2021 ◽  
Author(s):  
Annie Zhou ◽  
Shaun A Engelmann ◽  
Samuel A Mihelic ◽  
Alankrit Tomar ◽  
Ahmed M Hassan ◽  
...  
Keyword(s):  
Shot Noise ◽  
High Speed ◽  
Imaging Technique ◽  
Low Cost ◽  
Excitation Power ◽  
Acquisition Time ◽  
Signal To Noise ◽  
High Speed Imaging ◽  
Two Photon ◽  
Neurovascular Imaging

We demonstrate a simple, low-cost two-photon microscope design with both galvo-galvo and resonant-galvo scanning capabilities. We quantify and compare the signal-to-noise ratios and imaging speeds of the galvo-galvo and resonant-galvo scanning modes when used for murine neurovascular imaging. The two scanning modes perform as expected under shot-noise limited detection and are found to achieve comparable signal-to-noise ratios. Resonant-galvo scanning is capable of reaching desired signal-to-noise ratios using less acquisition time when higher excitation power can be used. Given equal excitation power and total pixel dwell time between the two methods, galvo-galvo scanning outperforms resonant-galvo scanning in image quality when detection deviates from being shot-noise limited.

scholarly journals High-speed imaging of transient metabolic dynamics using two-photon fluorescence lifetime imaging microscopy

Optica ◽  
2018 ◽  
Vol 5 (10) ◽  
pp. 1290 ◽  
Author(s):  
Andrew J. Bower ◽  
Joanne Li ◽  
Eric J. Chaney ◽  
Marina Marjanovic ◽  
Darold R. Spillman ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
High Speed ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
High Speed Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Large-scale drop test on perlite–metal syntactic foam

2018 ◽  
Vol 53 (4) ◽  
pp. 515-520 ◽  
Author(s):  
T Fiedler ◽  
M Taherishargh
Keyword(s):  
High Speed ◽  
Large Scale ◽  
Low Cost ◽  
Syntactic Foam ◽  
Drop Test ◽  
Passenger Vehicle ◽  
High Speed Imaging ◽  
Cellular Metal ◽  
Energy Absorbing ◽  
Impact Mass

Perlite–metal syntactic foam is a low-cost cellular metal intended for use in automotive impact protection. To test the viability of the material a 2.5 ton drop test was conducted. Impact mass and energy were selected to replicate the conditions of a frontal impact between a large passenger vehicle and a crash cushion. A hollow syntactic foam cylinder was manufactured to decelerate the drop weight in a controlled manner. Accelerometers and high-speed imaging were utilized to evaluate the performance of the energy absorbing element.

scholarly journals Measuring droplet fall speed with a high-speed camera: indoor accuracy and potential outdoor applications

2016 ◽  
Vol 9 (4) ◽  
pp. 1755-1766 ◽  
Author(s):  
Cheng-Ku Yu ◽  
Pei-Rong Hsieh ◽  
Sandra E. Yuter ◽  
Lin-Wen Cheng ◽  
Chia-Lun Tsai ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging Technique ◽  
High Speed Camera ◽  
High Speed Imaging ◽  
Ambient Flow ◽  
Mean Error ◽  
Challenging Issue ◽  
Size Relationship ◽  
Fall Speed ◽  
Moderate Cost

Abstract. Acquisition of accurate raindrop fall speed measurements outdoors in natural rain by means of moderate-cost and easy-to-use devices represents a long-standing and challenging issue in the meteorological community. Feasibility experiments were conducted to evaluate the indoor accuracy of fall speed measurements made with a high-speed camera and to evaluate its capability for outdoor applications. An indoor experiment operating in calm conditions showed that the high-speed imaging technique can provide fall speed measurements with a mean error of 4.1–9.7 % compared to Gunn and Kinzer's empirical fall-speed–size relationship for typical sizes of rain and drizzle drops. Results obtained using the same apparatus outside in summer afternoon showers indicated larger positive and negative velocity deviations compared to the indoor measurements. These observed deviations suggest that ambient flow and turbulence play a role in modifying drop fall speeds which can be quantified with future outdoor high-speed camera measurements. Because the fall speed measurements, as presented in this article, are analyzed on the basis of tracking individual, specific raindrops, sampling uncertainties commonly found in the widely adopted optical disdrometers can be significantly mitigated.

scholarly journals Low-Cost High-Speed Imaging System for Observing Vocal Fold Vibration in Voice Disorders

ORL ◽  
2012 ◽  
Vol 74 (4) ◽  
pp. 208-210 ◽  
Author(s):  
Kenichi Kaneko ◽  
Koichi Sakaguchi ◽  
Masato Inoue ◽  
Haruo Takahashi
Keyword(s):  
Vocal Fold ◽  
High Speed ◽  
Imaging System ◽  
Low Cost ◽  
Voice Disorders ◽  
High Speed Imaging ◽  
Vocal Fold Vibration

scholarly journals Measuring Droplet Fall Speed with a High-Speed Camera: Indoor Accuracy and Potential Outdoor Applications

2016 ◽  
Author(s):  
C.-K. Yu ◽  
P.- R. Hsieh ◽  
S. E. Yuter ◽  
L.- W. Cheng ◽  
C.- L. Tsai ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging Technique ◽  
High Speed Camera ◽  
High Speed Imaging ◽  
Ambient Flow ◽  
Mean Error ◽  
Challenging Issue ◽  
Rain Drop ◽  
Rain Drops ◽  
Fall Speed

Abstract. The acquisition of accurate rain drop fall speed measurements outdoors in natural rain represents a long-standing and challenging issue in the meteorological community. Feasibility experiments were conducted to evaluate the indoor accuracy of fall speed measurements made with a high-speed camera and to evaluate its capability for outdoor applications. An indoor experiment operating in calm conditions showed that the high-speed imaging technique can provide fall speed measurements with a mean error of 4.1~9.7% compared to Gunn and Kinzer’s empirical fall speed-size relationship for typical sizes of rain and drizzle drops. Results obtained using the same apparatus outside in summer afternoon showers indicated larger, positive and negative velocity deviations compared to the indoor measurements. These observed deviations suggest that ambient flow and turbulence play a role in modifying drop fall speeds which can be quantified with future outdoor high-speed camera measurements. Because the fall speed measurements, as presented in this article, are analyzed on the basis of tracking individual, specific rain drops, sampling uncertainties commonly found in the widely adopted optical disdrometers can be significantly mitigated.

Comparison of Atomization Characteristics of Jet A-1 and Alternative Aviation Fuels Using High-Speed Imaging Technique

2019 ◽  
Author(s):  
Manish Kumar ◽  
Srinibas Karmakar
Keyword(s):  
Surface Tension ◽  
High Speed ◽  
Alternative Fuels ◽  
Imaging Technique ◽  
Droplet Formation ◽  
Fuel Properties ◽  
Slight Variation ◽  
High Speed Imaging ◽  
Butyl Butyrate ◽  
Atomization Characteristics

Abstract Environmental pollution from gas turbine engines is becoming a serious concern recently because of the steep growth in the aviation sector globally. Therefore, potential alternative fuels which can partially or fully replace fossil-based jet fuel are getting significant attention. However, the search for suitable candidate fuels which can fulfill the requirement in terms of fuel properties and combustion performance is continuing. The present study deals with an experimental investigation of atomization characteristics of Jet A-1, butanol, and butyl butyrate in quiescent atmospheric air. A high-speed imaging technique has been adopted to make a comparison of ligament breakup characteristics and droplet formation of these alternative biofuels with that of Jet A-1. Various fuel properties, including density, viscosity, and surface tension, are compared. An effort is made to understand how the variation in fuel properties influences the atomization mechanism of each fuel. The surface tension seems to be similar for these three fuels with a slight variation in density. However, there is a significant variation in viscosity. Viscosity appears to play a major role in the difference observed in ligament length and droplet formation. Due to the higher viscosity of butanol, the droplet formation seems to be delayed compared to Jet A-1, whereas the lower viscosity of butyl butyrate promotes faster droplet formation. The effect of blending of these biofuels with Jet A-1 on atomization characteristics will be compared with that of Jet A-1.

A Novel High-Speed Imaging Technique to Predict the Macroscopic Spray Characteristics of Solution Based Pressurised Metered Dose Inhalers

2014 ◽  
Vol 31 (11) ◽  
pp. 2963-2974 ◽  
Author(s):  
Nicolas A. Buchmann ◽  
Daniel J. Duke ◽  
Sayed A. Shakiba ◽  
Daniel M. Mitchell ◽  
Peter J. Stewart ◽  
...  
Keyword(s):  
High Speed ◽  
Imaging Technique ◽  
Metered Dose Inhalers ◽  
High Speed Imaging ◽  
Spray Characteristics ◽  
Metered Dose ◽  
Pressurised Metered Dose Inhalers

Nanomanipulator Based on a High-Speed Atomic Force Microscopy

2012 ◽  
Vol 516 ◽  
pp. 396-401
Author(s):  
Itsuhachi Ishisaki ◽  
Yuya Ohashi ◽  
Tatsuo Ushiki ◽  
Futoshi Iwata
Keyword(s):  
Atomic Force Microscopy ◽  
Real Time ◽  
High Speed ◽  
Imaging Technique ◽  
Frame Rate ◽  
High Speed Imaging ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Short Time

We developed a real-time nanomanipulation system based on high-speed atomic force microscopy (HS-AFM). During manipulation, the operation of the manipulation is momentarily interrupted for a very short time for high-speed imaging; thus, the topographical image of the fabricated surface is periodically updated during the manipulation. By using a high-speed imaging technique, the interrupting time could be much reduced during the manipulation; as a result, the operator almost does not notice the blink time of the interruption for imaging during the manipulation. As for the high-speed imaging technique, we employed a contact-mode HS-AFM to obtain topographic information through the instantaneous deflection of the cantilever during high-speed scanning. By using a share motion PZT scanner, the surface could be imaged with a frame rate of several fps. Furthermore, the high-speed AFM was coupled with a haptic device for human interfacing. By using the system, the operator can move the AFM probe into any position on the surface and feel the response from the surface during manipulation. As a demonstration of the system, nanofabrication under real-time monitoring was performed. This system would be very useful for real-time nanomanipulation and fabrication of sample surfaces.

Sign in / Sign up

Export Citation Format

Share Document