scholarly journals Towards sustainable bioplastic production in resource limited environments using the photoferroautotrophic and photoelectroautotrophic bacteriumRhodopseudomonas palustrisTIE-1

2017 ◽  
Author(s):  
Tahina Onina Ranaivoarisoa ◽  
Karthikeyan Rengasamy ◽  
Michael S. Guzman ◽  
Rajesh Singh ◽  
Arpita Bose
Keyword(s):  
Energy Source ◽  
Carbon Source ◽  
Organic Carbon ◽  
Ferrous Iron ◽  
Rhodopseudomonas Palustris ◽  
Electron Source ◽  
Light Energy ◽  
Attractive Alternative ◽  
Metabolic Versatility ◽  
Phb Production

ABSTRACTBioplastics are an attractive alternative to petroleum-derived plastics because of the harmful environmental effects of conventional plastics and the impending fossil fuel crisis. Polyhydroxybutyrate (PHB) is a well-known bioplastic that is produced by several microbes using organic carbon sources. Autotrophic (using carbon dioxide or CO2) PHB production is reported for only a few organisms. Sustainable PHB bioproduction using other autotrophic microbes needs to be explored.Rhodopseudomonas palustris, a metabolically versatile purple non-sulfur bacterium (PNSB) is known to produce PHBs under photoheterotrophic conditions.Rhodopseudomonas palustrisstrain TIE-1 demonstrates extended metabolic versatility by using electron sources such as ferrous iron and poised electrodes for photoautotrophy. Here we report the ability of TIE-1 to produce PHB under photoferroautotrophic (light - energy source, ferrous iron - electron source and CO2- carbon source) and photoelectroautotrophic (light - energy source, poised electrodes - electron source and CO2- carbon source) growth conditions. PHB accumulation was observed both under nitrogen (N2) fixing and non-N2fixing conditions. For comparison, we determined PHB production under chemoheterotrophic, photoheterotrophic and photoautotrophic conditions using hydrogen as the electron donor. Photoferroautotrophic and photoelectroautotrophic PHB production was on par with that observed from organic carbon substrates such as butyrate. PHB production increased during N2fixation under photoheterotrophic conditions but not during photoautotrophic growth. Electron microscopy confirmed that TIE-1 cells accumulate PHBs internally under the conditions that showed highest production. However, gene expression analysis suggests that PHB cycle genes are not differentially regulated despite observable changes in biopolymer production.

scholarly journals Alkalinity, and Not the Oxidation State of the Organic Substrate, Is the Key Factor in Domestic Wastewater Treatment by Mixed Cultures of Purple Phototrophic Bacteria

Resources ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 88
Author(s):  
Carol Nairn ◽  
Iván Rodríguez ◽  
Yolanda Segura ◽  
Raúl Molina ◽  
Natalia González-Benítez ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Carbon Source ◽  
Organic Carbon ◽  
Oxidation State ◽  
Rhodopseudomonas Palustris ◽  
Domestic Wastewater ◽  
Phototrophic Bacteria ◽  
Efficient Treatment ◽  
Domestic Wastewater Treatment ◽  
Organic Carbon Source

Domestic wastewater treatment by purple phototrophic bacteria (PPB) is based on the assimilative uptake of organics and nutrients into the bacterial biomass. Thereby, it strongly depends on the carbon/nutrients ratio of the wastewater. The physiological COD/N/P ratio for PPB growth in domestic wastewater makes the addition of an external organic carbon source necessary in order to allow for an efficient process. However, PPB need a source of alkalinity (as CO2) to grow on reduced organics that serves as an electron acceptor since biohydrogen production (an alternative electron sink) is inhibited by ammonium. A preliminary experiment showed that high nutrients-loading wastewater was limited by CO2 imbalance, leading to poor removal efficiencies. Subsequently, the effect of the oxidation state of the organics added as external organic carbon sources to PPB reactors treating low nutrients-loading domestic wastewater has been analyzed. Three organics were used as additives to PPB development in four consecutive batches: acetate (more oxidized), ethanol and butyrate (more reduced). The PPB population was settled and the general performance under the three situations, in terms of organics, N and P assimilation, and growth kinetics was not significantly different irrespective of the external organic carbon source. The reactors were dominated by PPB, though reduced organics allowed for dominance of Rhodopseudomonas palustris, whereas oxidized organics caused co-dominance of R. palustris and Rhodobacter capsulatus. Thereby, alkalinity (as bicarbonate), and not the oxidation state of the organics, is the key parameter for the efficient treatment of domestic wastewater by PPB.

Effect of different frequencies of the addition of Brachionus plicatilis on the performance of Litopenaeus vannamei in a nursery biofloc system with rice bran (anaerobic and aerobic) as an organic carbon source

Aquaculture ◽  
2021 ◽  
pp. 736669
Author(s):  
Rildo José Vasconcelos de Andrade Brazil ◽  
Elizabeth Pereira dos Santos ◽  
Gisely Karla de Almeida Costa ◽  
Clarissa Vilela Figueiredo Campos ◽  
Suzianny Maria Bezerra Cabral da Silva ◽  
...  
Keyword(s):  
Carbon Source ◽  
Organic Carbon ◽  
Rice Bran ◽  
Litopenaeus Vannamei ◽  
Brachionus Plicatilis ◽  
Organic Carbon Source

Model Study To Assess Softwood Hemicellulose Hydrolysates as the Carbon Source for PHB Production inParaburkholderia sacchariIPT 101

2017 ◽  
Vol 19 (1) ◽  
pp. 188-200 ◽  
Author(s):  
Karolin Dietrich ◽  
Marie-Josée Dumont ◽  
Timothy Schwinghamer ◽  
Valérie Orsat ◽  
Luis F. Del Rio
Keyword(s):  
Carbon Source ◽  
Model Study ◽  
Phb Production

Synergistic Experimental and Computational Approach Identifies Novel Strategies for PHB Overproduction

2021 ◽  
Author(s):  
Adil Alsiyabi ◽  
Brandi Brown ◽  
Cheryl Immethun ◽  
Mark Wilkins ◽  
Rajib Saha
Keyword(s):  
Engineering Design ◽  
Rhodopseudomonas Palustris ◽  
Maximum Yield ◽  
Computational Approach ◽  
Coniferyl Alcohol ◽  
Breakdown Product ◽  
Design Strategies ◽  
Metabolic Factors ◽  
Model Driven ◽  
Phb Production

Abstract Polyhydroxybutyrate (PHB) is a sustainable bioplastic produced by bacteria that is a potential replacement for conventional plastics. This study delivers an integrated experimental and computational modeling approach to decipher metabolic factors controlling PHB production and offers engineering design strategies to boost production. In the metabolically robust Rhodopseudomonas palustris CGA009, PHB production significantly increased when grown on the carbon- and electron-rich lignin breakdown product p-coumarate (C9H8O3) compared to acetate when the same amount of carbon was supplied. However, the maximum yield did not improve further when grown on coniferyl alcohol (C10H12O3). In order to obtain a systems-level understanding of factors driving PHB yield, a model-driven investigation was performed. The model yielded several engineering design strategies including utilizing reduced, high molecular weight substrates that bypass the thiolase reaction. Overall, these findings uncover key parameters controlling PHB production and design strategies that can potentially be expanded to any bacterium for optimizing PHB production.

scholarly journals Poly-β-Hydroxybutyrate (PHB) Production By Amylolytic Micrococcus sp. PG1 Isolated From Soil Polluted Arrowroot Starch Waste

2015 ◽  
Vol 19 (1) ◽  
pp. 56
Author(s):  
Sebastian Margino ◽  
Erni Martani ◽  
Andriessa Prameswara
Keyword(s):  
Carbon Source ◽  
Internal Energy ◽  
Organic Polymer ◽  
Ftir Analysis ◽  
Phb Production ◽  
Alternative Carbon Source ◽  
Arrowroot Starch ◽  
Physiological Characters

Poly-β-hydroxybutyrate (PHB) production from amylolytic Micrococcus sp. PG1. Poly-β-hydroxybutyrate(PHB) is an organic polymer, which synthesized by many bacteria and serves as internal energy. PHB ispotential as future bioplastic but its price is very expensive due to glucose usage in PHB industry. Thedevelopment of PHB production using starch as an alternative carbon source has been conducted to reducethe dependence of glucose in PHB production. In this study, amylolytic bacteria from arrowroot processingsite were screened quantitavely based on amylase specifi c activity and PHB producing ability. The result of thestudy showed that among of 24 amylolytic isolates, 12 isolates of them were able to accumulate PHB rangedfrom 0,68-11,65% (g PHB/g cdw). The highest PHB production from substrate arrowroot starch was PG1 andafter optimization resulted in increasing of PHB production up to 16,8% (g PHB/g cdw) 40 hours incubationtime. Based on morphological, biochemical and physiological characters, the PG1 isolate was identifi ed asMicrococcus sp. PG1. Result of the FTIR analysis of produced polymer by Micrococcus sp. PG1 was indicatedas poly-β- hydroxybutyrate (PHB)

scholarly journals Mixotrophic cultivation of Chlorella vulgaris using industrial dairy waste as organic carbon source

2012 ◽  
Vol 118 ◽  
pp. 61-66 ◽  
Author(s):  
Ana P. Abreu ◽  
Bruno Fernandes ◽  
António A. Vicente ◽  
José Teixeira ◽  
Giuliano Dragone
Keyword(s):  
Carbon Source ◽  
Organic Carbon ◽  
Chlorella Vulgaris ◽  
Mixotrophic Cultivation ◽  
Dairy Waste ◽  
Organic Carbon Source
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