Comparison between three concentration techniques for diagnosing intestinal parasites

ABSTRACTBackgroundIntestinal parasitoses still are a noticeable threat to public health. The direct diagnosis of such parasites requires the use of concentration techniques, whose sensitivities for protozoan cysts and helminth eggs are far from equal.AimTo compare the Willis, Ritchie and Bailenger concentration techniques in terms of parasite recovery, cost, time, and biosafety.MethodsThis prospective study analysed 236 stool specimens for intestinal parasites using the direct wet smear and the above-mentioned concentration techniques applied separately.ResultsBiphasic techniques identified significantly more positive specimens for intestinal parasites than the Willis technique, the latter leading to less concentrated and more altered parasitic elements on microscopy. No statistically significant difference emerged from comparing Ritchie’s and Bailenger’s methods. The Willis technique was the safest, yet the costliest and the most time-consuming of the studied methods.ConclusionsEven though the hazardous reagents employed may raise legitimate concerns over their health implications, biphasic techniques prove to be uncostly, quick to perform, and highly sensitive for detecting faecal parasites, therefore ensuring a safe diagnosis for routine stool examinations.

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Assessment of Intestinal Parasites of Commercial Layers in Selected Local Government Areas of Rivers State, Nigeria and Their Public Health Implications

Current Trends in Biomedical Engineering & Biosciences ◽

10.19080/ctbeb.2018.11.555822 ◽

2018 ◽

Vol 11 (5) ◽

Author(s):

Wokem GN

Keyword(s):

Public Health ◽

Local Government ◽

Intestinal Parasites ◽

Health Implications ◽

Rivers State

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Evaluation of Formalin — Acetone Sedimentation in the Concentration of Stool for Intestinal Parasites

Tropical Doctor ◽

10.1177/004947550303300315 ◽

2003 ◽

Vol 33 (3) ◽

pp. 163-164 ◽

Cited By ~ 5

Author(s):

S C Parija ◽

S Bhattacharya ◽

P Padhan ◽

M R Shivaprakash

Keyword(s):

Recovery Rate ◽

Intestinal Parasites ◽

Wet Mount ◽

Significant Difference ◽

Cotton Blue ◽

Stool Specimens

Formalin-acetone sedimentation was compared with the formalin-ether method for the concentration of stool for intestinal parasites. Of 80 stool specimens, 45 (56.25%) were positive for parasites by the formalin-acetone method. The figures for the two methods were formalin-ether 35 (43.75%) and for the direct lacto-phenol cotton blue wet mount method 17 (21.25%). There was no statistically significant difference in the parasite recovery rate between the two methods. Acetone is more stable, safer, and a cheaper fat solvent and promises to be a useful alternative to ether.

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Linguatula serrata(Porocephalida: Linguatulidae) Infection among Client-Owned Dogs in Jalingo, North Eastern Nigeria: Prevalence and Public Health Implications

Journal of Parasitology Research ◽

10.1155/2014/916120 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 8

Author(s):

Oseni Saheed Oluwasina ◽

Onyiche Emmanuel ThankGod ◽

Omonuwa Omojefe Augustine ◽

Fufa Ido Gimba

Keyword(s):

Public Health ◽

Human Health ◽

Central Africa ◽

Linguatula Serrata ◽

Health Implications ◽

Preventative Measures ◽

Significant Difference ◽

High Prevalence ◽

North Eastern

Pentastomiasis is a parasitic zoonosis endemic to western and central Africa. This study was undertaken to determine the prevalence and public health implications of Linguatulosis in client-owned dogs in Jalingo, North Eastern Nigeria. Seven hundred and seventy seven (777) dogs brought for treatment at the hospital were subjected to buccal (sublingual) examination for pentastomiasis. Parameters such as age, sex, and breeds were determined. Also, the months of the year were taken into consideration. An overall prevalence of 37.45% was recorded. Of the 477 dogs examined in 2010, 184 were positive representing prevalence of 38.57% and in 2011 107 were positive representing prevalence of 35.67%. The infection was higher in the male than in the female which does not differ significantly (P>0.05). There was no significant difference between sexes (P>0.05). However, significant difference (P<0.05) was observed between breeds and age of dogs examined. Season did not have much influence on the prevalence of Linguatulosis. The high prevalence of Linguatulosis in dogs and other animals found in this study highlights the need of improving preventative measures to reduce the rate of infection, which may pose a hazard to human health.

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Isolation of Intestinal Parasites of Public Health Importance from Cockroaches (Blattella germanica) in Jimma Town, Southwestern Ethiopia

Journal of Parasitology Research ◽

10.1155/2014/186240 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 7

Author(s):

Haji Hamu ◽

Serkadis Debalke ◽

Endalew Zemene ◽

Belay Birlie ◽

Zeleke Mekonnen ◽

...

Keyword(s):

Public Health ◽

Intestinal Parasites ◽

Blattella Germanica ◽

Gut Contents ◽

Mechanical Transmission ◽

Public Health Importance ◽

Carriage Rate ◽

Southwestern Ethiopia ◽

Significant Difference ◽

Jimma Town

Cockroaches are claimed to be mechanical transmitters of disease causing microorganisms such as intestinal parasites, bacteria, fungi, and viruses. This study assessed the potential of the German cockroachBlattella germanicain the mechanical transmission of intestinal parasites of public health importance. A total of 2010 cockroaches were collected from 404 households in Jimma Town, southwestern Ethiopia. All the collected cockroaches were identified to species asB. germanica. The contents of their gut and external body parts were examined for the presence of intestinal parasites. Overall, 152 (75.6%) of the 210 batches were found to harbor at least one species of human intestinal parasite.Ascaris lumbricoides, Trichuris trichiura, Taenia spp, Strongyloides-like parasite,Entamoeba histolytica/dispar/moshkovski, Giardia duodenalisandBalantidium coliwere detected from gut contents. Moreover, parasites were also isolated from the external surface in 22 (10.95%) of the batches. There was significant difference in parasite carriage rate of the cockroaches among the study sites (P=0.013). In conclusion,B. germanicawas found to harbor intestinal parasites of public health importance. Hence, awareness on the potential role of cockroaches in the mechanical transmission of human intestinal parasites needs to be created. Moreover, further identification of the Strongyloides-like worm is required using molecular diagnostics.

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Understanding the Public Health Implications of Prisoner Reentry in California: Summary

PsycEXTRA Dataset ◽

10.1037/e527062010-001 ◽

2009 ◽

Cited By ~ 1

Author(s):

Lois M. Davis ◽

Nancy Nicosia ◽

Adrian Overton ◽

Lisa Miyashiro ◽

Kathryn Pitkin Derose ◽

...

Keyword(s):

Public Health ◽

Prisoner Reentry ◽

Health Implications ◽

The Public

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Subthreshold Depression in Older Adults: Epidemiology, Illness Course, and Public Health Implications

PsycEXTRA Dataset ◽

10.1037/e566572011-008 ◽

2011 ◽

Author(s):

T. W. Meeks ◽

I. V. Vahia ◽

H. Lavretsky ◽

G. Kulkarni ◽

D. V. Jeste

Keyword(s):

Public Health ◽

Older Adults ◽

Subthreshold Depression ◽

Health Implications

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A highly sensitive sandwich enzyme immunoassay of urinary growth hormone in children with short stature, Turner's syndrome, and simple obesity

Acta Endocrinologica ◽

10.1530/acta.0.1210513 ◽

1989 ◽

Vol 121 (4) ◽

pp. 513-519 ◽

Cited By ~ 6

Author(s):

Hiroshi Tomita ◽

Masamichi Ogawa ◽

Takashi Kamijo ◽

Osamu Mori ◽

Eiji Ishikawa ◽

...

Keyword(s):

Short Stature ◽

Enzyme Immunoassay ◽

Gh Deficiency ◽

Urine Samples ◽

Turner's Syndrome ◽

Turner’S Syndrome ◽

Highly Sensitive ◽

Significant Difference ◽

Simple Obesity ◽

Sandwich Enzyme Immunoassay

Abstract. GH values were determined by a highly sensitive sandwich enzyme immunoassay in the 1st morning and/or 24-h accumulated urine samples in 94 children (short stature 70, including 14 with complete GH deficiency, 9 with partial GH deficiency, and 47 with GH-normal short stature; Turner's syndrome, 10, and simple obesity, 14). GH values were also determined in the 2nd to 4th urine samples taken on the same day together with the 1st morning urine in 5 of them. GH values in the 1st morning urine correlated significantly with those of the 24-h urine and with serum peak and mean GH values during nocturnal sleep as a physiological GH secretion test. The 2nd to 4th urines had lower GH concentrations than the 1st morning urine. The GH value of the 1st morning urine in complete GH deficiency was significantly lower than those in GH-normal short stature, partial GH deficiency and Turner's syndrome. However, no significant difference was detected in urinary GH values between complete GH deficiency and simple obesity. We conclude that 1st morning urinary GH estimation may be useful for differentiation of complete GH deficiency from other causes of short stature, but may be difficult for the distinction between complete GH deficiency and obesity with normal GH secretory ability.

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What are Hidden Facts behind Intestinal Parasitic Infections in Ilam City?

Infectious Disorders - Drug Targets ◽

10.2174/1871526518666180508125418 ◽

2019 ◽

Vol 19 (3) ◽

pp. 284-287

Author(s):

S. Viesy ◽

J. Abdi ◽

Z. Rezaei

Keyword(s):

Public Health ◽

Intestinal Parasites ◽

Parasitic Infection ◽

Public Health Education ◽

Health Problems ◽

Parasitic Infections ◽

Blastocystis Hominis ◽

Cross Sectional ◽

Intestinal Parasitic Infections ◽

Number Of Patients

Background: Intestinal parasitic infections are the one of the most common health problems in developing countries. Objective: A number of patients die annually due to complications caused by these parasites.Therefore, the aim of this study was to investigate the rate and type of parasitic infections, determine the factors affecting them in Ilam city and also provide strategies to prevent them.In this descriptive cross-sectional study conducted in one of the Ilam labs in 2016, 417 stool specimens were randomly collected. All specimens were examined using direct and ethanol formaldehyde.Suspect specimens were examined using Trichrom staining. Demographic information was also recorded in a questionnaire, and finally the results were analyzed using statistical software SPSS 20.The data were then compared with Chi-square test. Results: Out of the 417 patients examined, 59 (14.1%) were infected with intestinal parasites. The type of parasitic infection in 9.4% was Blastocystis hominis, 3.6% Entamoeba coli, 0.5% Entamoeba histolytica, 0.5% Giardia and 0.2% Trichomonas hominis. Conclusion: Despite the improvement of public health, parasitic infections are still considered as one of the health problems in the city of Ilam. Therefore, proper planning, public health education, raising the level of health in the area and the provision of safe drinking water are some of the ways to reduce parasitic infections in the region.

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