scholarly journals Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain

2018 ◽  
Author(s):  
J. Luzuriaga ◽  
O. Pastor-Alonso ◽  
J.M. Encinas ◽  
F. Unda ◽  
G. Ibarretxe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Dental Pulp ◽  
Vascular Endothelial Cell ◽  
Brain Cell ◽  
Dental Pulp Stem Cells ◽  
Vascular Endothelial ◽  
Cell Therapies ◽  
Human Dental Pulp ◽  
A Cell

SUMMARYDental Pulp Stem Cells (DPSCs) have a demonstrated capacity to acquire neuronal-like phenotypes, suggesting their use in brain cell therapies. In the present work, we wanted to address the phenotypic fate of adult DPSCs cultured in Neurocult media (Stem Cell Technologies), a cell culture medium without serum routinely used for the expansion of adult neural stem cells (NSCs). Our results showed for the first time, that non-genetically modified adult DPSCs cultured with Neurocult generated neurosphere-like dentospheres expressing the NSC markers Nestin and GFAP, but also the vascular endothelial cell marker CD31. One month post-intracranial graft into athymic nude mice, human CD31+ or Nestin+ DPSC-derived cells were found tightly associated with brain blood vessels increasing their laminin staining. These results suggest that DPSCs integrated and contributed to an increased generation of neovasculature within brain tissue and that Neurocult medium constituted a fast and efficient way to obtain endothelial cells from human DPSCs.

A Cell-permeable Fusion Protein for the Mineralization of Human Dental Pulp Stem Cells

2011 ◽  
Vol 91 (1) ◽  
pp. 90-96 ◽  
Author(s):  
J.S. Suh ◽  
K.S. Kim ◽  
J.Y. Lee ◽  
Y.J. Choi ◽  
C.P. Chung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Fusion Protein ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp ◽  
A Cell

scholarly journals Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain

2019 ◽  
Vol 10 ◽  
Author(s):  
Jon Luzuriaga ◽  
Oier Pastor-Alonso ◽  
Juan Manuel Encinas ◽  
Fernando Unda ◽  
Gaskon Ibarretxe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Mouse Brain ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Human Dental Pulp

scholarly journals Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering

2011 ◽  
Vol 23 (3) ◽  
pp. 325-332 ◽  
Author(s):  
V.T. Sakai ◽  
M.M. Cordeiro ◽  
Z. Dong ◽  
Z. Zhang ◽  
B.D. Zeitlin ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Endothelial Cells ◽  
Dental Pulp ◽  
Vascular Endothelial Cells ◽  
Dental Pulp Stem Cells ◽  
Vascular Endothelial ◽  
Pulp Tissue ◽  
Differentiation Potential ◽  
Dental Pulp Tissue

Multipotency is a defining characteristic of post-natal stem cells. The human dental pulp contains a small subpopulation of stem cells that exhibit multipotency, as demonstrated by their ability to differentiate into odontoblasts, neural cells, and vascular endothelial cells. These discoveries highlight the fundamental role of stem cells in the biology of the dental pulp and suggest that these cells are uniquely suited for dental pulp tissue-engineering purposes. The availability of experimental approaches specifically designed for studies of the differentiation potential of dental pulp stem cells has played an important role in these discoveries. The objective of this review is to describe the development and characterization of the Tooth Slice/Scaffold Model of Dental Pulp Tissue Engineering. In addition, we discuss the multipotency of dental pulp stem cells, focusing on the differentiation of these cells into functional odontoblasts and into vascular endothelial cells.

scholarly journals Effects of concentrated growth factor on proliferation, migration, and differentiation of human dental pulp stem cells in vitro

2018 ◽  
Vol 9 ◽  
pp. 204173141881750 ◽  
Author(s):  
Runze Jin ◽  
Guangtai Song ◽  
Jihua Chai ◽  
Xiaohui Gou ◽  
Guohua Yuan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Cell Differentiation ◽  
Growth Factor ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Vascular Endothelial ◽  
Human Dental Pulp ◽  
Dental Pulp Stem Cell ◽  
Endothelial Growth Factor

Concentrated growth factor, a novel autologous plasma extract, contained various growth factors which promoted tissue regeneration. In this study, we aimed to investigate the biological effects of concentrated growth factor on human dental pulp stem cells. The microstructure and biocompatibility of concentrated growth factor scaffolds were evaluated by scanning electron microscopy. Cell proliferation and migration, odontoblastic and endothelial cell differentiation potential were assessed after exposing dental pulp stem cells to different concentrations (5%, 10%, 20%, 50%, or 80%) of concentrated growth factor extracts. The results revealed that concentrated growth factor scaffolds possessed porous fibrin network with platelets and leukocytes, and showed great biocompatibility with dental pulp stem cells. Higher cell proliferation rates were detected in the concentrated growth factor–treated groups in a dose-dependent manner. Interestingly, in comparison to the controls, the low doses (<50%) of concentrated growth factor increased cell migration, alkaline phosphatase activity, and mineralized tissue deposition, while the cells treated in high doses (50% or 80%) showed no significant difference. After stimulating cell differentiation, the expression levels of dentin matrix protein-1, dentin sialophosphoprotein, vascular endothelial growth factor receptor-2 and cluster of differentiation 31 were significantly upregulated in concentrated growth factor–supplemented groups than those of the controls. Furthermore, the dental pulp stem cell–derived endothelial cells co-induced by 5% concentrated growth factor and vascular endothelial growth factor formed the most amount of mature tube-like structures on Matrigel among all groups, but the high-dosage concentrated growth factor exhibited no or inhibitory effect on cell differentiation. In general, our findings confirmed that concentrated growth factor promoted cell proliferation, migration, and the dental pulp stem cell–mediated dentinogenesis and angiogenesis process, by which it might act as a growth factor–loaded scaffold to facilitate dentin–pulp complex healing.

scholarly journals Inhibition of TGF-β Signaling in SHED Enhances Endothelial Differentiation

2017 ◽  
Vol 97 (2) ◽  
pp. 218-225 ◽  
Author(s):  
J.G. Xu ◽  
T. Gong ◽  
Y.Y. Wang ◽  
T. Zou ◽  
B.C. Heng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Vascular Endothelial Growth Factor ◽  
Endothelial Cells ◽  
Growth Factor ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Endothelial Differentiation ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Specific Ligand

Low efficiency of deriving endothelial cells (ECs) from adult stem cells hampers their utilization in tissue engineering studies. The purpose of this study was to investigate whether suppression of transforming growth factor beta (TGF-β) signaling could enhance the differentiation efficiency of dental pulp–derived stem cells into ECs. We initially used vascular endothelial growth factor A (VEGF-A) to stimulate 2 dental pulp–derived stem cells (dental pulp stem cells and stem cells from human exfoliated deciduous teeth [SHED]) and compared their differentiation capacity into ECs. We further evaluated whether the vascular endothelial growth factor receptor I (VEGF-RI)-specific ligand placental growth factor-1 (PlGF-1) could mediate endothelial differentiation. Finally, we investigated whether the TGF-β signaling inhibitor SB-431542 could enhance the inductive effect of VEGF-A on endothelial differentiation, as well as the underlying mechanisms involved. ECs differentiated from dental pulp–derived stem cells exhibited the typical phenotypes of primary ECs, with SHED possessing a higher endothelial differentiation potential than dental pulp stem cells. VEGFR1-specific ligand-PLGF exerted a negligible effect on SHED-ECs differentiation. Compared with VEGF-A alone, the combination of VEGF-A and SB-431542 significantly enhanced the endothelial differentiation of SHED. The presence of SB-431542 inhibited the phosphorylation of Suppressor of Mothers Against Decapentaplegic 2/3 (SMAD2/3), allowing for VEGF-A-dependent phosphorylation and upregulation of VEGFR2. Our results indicate that the combination of VEGF-A and SB-431542 could enhance the differentiation of dental pulp–derived stem cells into endothelial cells, and this process is mediated through enhancement of VEGF-A-VEGFR2 signaling and concomitant inhibition of TGF-β-SMAD2/3 signaling.

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