scholarly journals Validation and characterization of a DNA methylation alcohol biomarker across the life course

2019 ◽  
Author(s):  
Paul Darius Yousefi ◽  
Rebecca Richmond ◽  
Ryan Langdon ◽  
Andrew Ness ◽  
Chunyu Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Parents And Children ◽  
Average Alcohol ◽  
Alcohol Biomarker ◽  
Audit Score ◽  
Potential Applications ◽  
The Life Course ◽  
Offspring Generation ◽  
First Time

AbstractRecently, an alcohol predictor was developed using DNA methylation at 144 CpG sites (DNAm-Alc) as a biomarker for improved clinical or epidemiologic assessment of alcohol-related ill health. We validate the performance and characterize the drivers of this DNAm-Alc for the first time in independent populations. In N=1,049 parents from the Avon Longitudinal Study of Parents and Children (ALSPAC) Accessible Resource for Integrated Epigenomic Studies (ARIES) at midlife, we found DNAm-Alc explained 7.6% of the variation in alcohol intake, roughly half of what had been reported previously, and interestingly explained a larger 9.8% of AUDIT score, a scale of alcohol use disorder. Explanatory capacity in participants from the offspring generation of ARIES measured during adolescence was much lower. However, DNAm-Alc explained 14.3% of the variation in replication using the Head and Neck 5000 (HN5000) clinical cohort that had higher average alcohol consumption. To investigate whether this relationship was being driven by genetic and/or earlier environment confounding we examined how earlier vs. concurrent DNAm-Alc measures predicted AUDIT scores. In both ARIES parental and offspring generations, we observed associations between AUDIT and concurrent, but not earlier DNAm-Alc, suggesting independence from genetic and stable environmental contributions. The stronger relationship between DNAm-Alcs and AUDIT in parents at midlife compared to adolescents despite similar levels of consumption suggests that DNAm-Alc likely reflects long-term patterns of alcohol abuse. Such biomarkers may have potential applications for biomonitoring and risk prediction, especially in cases where reporting bias is a concern.

scholarly journals Validation and characterisation of a DNA methylation alcohol biomarker across the life course

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Paul Darius Yousefi ◽  
Rebecca Richmond ◽  
Ryan Langdon ◽  
Andrew Ness ◽  
Chunyu Liu ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Alcohol Use ◽  
Parents And Children ◽  
Average Alcohol ◽  
Alcohol Biomarker ◽  
Audit Score ◽  
Potential Applications ◽  
The Life Course ◽  
Offspring Generation ◽  
First Time

Abstract Background Recently, an alcohol predictor was developed using DNA methylation at 144 CpG sites (DNAm-Alc) as a biomarker for improved clinical or epidemiologic assessment of alcohol-related ill health. We validate the performance and characterise the drivers of this DNAm-Alc for the first time in independent populations. Results In N = 1049 parents from the Avon Longitudinal Study of Parents and Children (ALSPAC) Accessible Resource for Integrated Epigenomic Studies (ARIES) at midlife, we found DNAm-Alc explained 7.6% of the variation in alcohol intake, roughly half of what had been reported previously, and interestingly explained a larger 9.8% of Alcohol Use Disorders Identification Test (AUDIT) score, a scale of alcohol use disorder. Explanatory capacity in participants from the offspring generation of ARIES measured during adolescence was much lower. However, DNAm-Alc explained 14.3% of the variation in replication using the Head and Neck 5000 (HN5000) clinical cohort that had higher average alcohol consumption. To investigate whether this relationship was being driven by genetic and/or earlier environment confounding, we examined how earlier versus concurrent DNAm-Alc measures predicted AUDIT scores. In both ARIES parental and offspring generations, we observed associations between AUDIT and concurrent, but not earlier DNAm-Alc, suggesting independence from genetic and stable environmental contributions. Conclusions The stronger relationship between DNAm-Alcs and AUDIT in parents at midlife compared to adolescents despite similar levels of consumption suggests that DNAm-Alc likely reflects long-term patterns of alcohol abuse. Such biomarkers may have potential applications for biomonitoring and risk prediction, especially in cases where reporting bias is a concern.

scholarly journals Association between Breastfeeding and DNA Methylation over the Life Course: Findings from the Avon Longitudinal Study of Parents and Children (ALSPAC)

Nutrients ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 3309 ◽  
Author(s):  
Fernando Pires Hartwig ◽  
George Davey Smith ◽  
Andrew J. Simpkin ◽  
Cesar Gomes Victora ◽  
Caroline L. Relton ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Longitudinal Study ◽  
Negative Control ◽  
Childhood And Adolescence ◽  
Long Term Effects ◽  
Parents And Children ◽  
Short And Long Term ◽  
The Life Course ◽  
Avon Longitudinal Study

Background: Breastfeeding is associated with short and long-term health benefits. Long-term effects might be mediated by epigenetic mechanisms, yet the literature on this topic is scarce. We performed the first epigenome-wide association study of infant feeding, comparing breastfed vs non-breastfed children. We measured DNA methylation in children from peripheral blood collected in childhood (age 7 years, N = 640) and adolescence (age 15–17 years, N = 709) within the Accessible Resource for Integrated Epigenomic Studies (ARIES) project, part of the larger Avon Longitudinal Study of Parents and Children (ALSPAC) cohort. Cord blood methylation (N = 702) was used as a negative control for potential pre-natal residual confounding. Results: Two differentially-methylated sites presented directionally-consistent associations with breastfeeding at ages 7 and 15–17 years, but not at birth. Twelve differentially-methylated regions in relation to breastfeeding were identified, and for three of them there was evidence of directional concordance between ages 7 and 15–17 years, but not between birth and age 7 years. Conclusions: Our findings indicate that DNA methylation in childhood and adolescence may be predicted by breastfeeding, but further studies with sufficiently large samples for replication are required to identify robust associations.

scholarly journals Association between breastfeeding and DNA methylation over the life course: findings from the Avon Longitudinal Study of Parents and Children (ALSPAC)

2019 ◽  
Author(s):  
Fernando Pires Hartwig ◽  
George Davey Smith ◽  
Andrew J Simpkin ◽  
Cesar Gomes Victora ◽  
Caroline L. Relton ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Longitudinal Study ◽  
Negative Control ◽  
Childhood And Adolescence ◽  
Long Term Effects ◽  
Parents And Children ◽  
Short And Long Term ◽  
The Life Course ◽  
Avon Longitudinal Study

AbstractBackgroundBreastfeeding is associated with short and long-term health benefits. Long-term effects might be mediated by epigenetic mechanisms, yet a recent systematic review indicated that the literature on this topic is scarce. We performed the first epigenome-wide association study of infant feeding, comparing breastfed vs non-breastfed children. We measured DNA methylation in children from peripheral blood collected in childhood (age 7, N=640) and adolescence (age 15-17, N=709) within the Accessible Resource for Integrated Epigenomic Studies (ARIES) project, part of the larger Avon Longitudinal Study of Parents and Children (ALSPAC) cohort. Cord blood methylation (N=702) was used as a negative control for potential pre-natal residual confounding.ResultsTwo differentially-methylated sites presented directionally-consistent associations with breastfeeding at ages 7 and 15-17, but not at birth. Twelve differentially-methylated regions in relation to breastfeeding were identified, and for three of them there was evidence of directional concordance between ages 7 and 15-17, but not between birth and age 7.ConclusionsOur findings indicate that DNA methylation in childhood and adolescence may be predicted by breastfeeding, but further studies with sufficiently large samples for replication are required to identify robust associations.

scholarly journals Associations between indicators of socioeconomic position and DNA methylation: A systematic review

2021 ◽  
Author(s):  
Janine K. Cerutti ◽  
Alexandre A. Lussier ◽  
Yiwen Zhu ◽  
Jiaxuan Liu ◽  
Erin C. Dunn
Keyword(s):  
Systematic Review ◽  
Dna Methylation ◽  
Life Course ◽  
Socioeconomic Position ◽  
Prenatal Development ◽  
Future Research ◽  
Socioeconomic Environment ◽  
The Life Course ◽  
Study Designs

AbstractSocioeconomic position (SEP) is a major determinant of health across the life course. Yet, little is known about the biological mechanisms explaining this relationship. One possible explanation is through an epigenetic process called DNA methylation (DNAm), wherein the socioeconomic environment causes no alteration in the DNA sequence but modifies gene activity, gene expression, and therefore long-term health. To understand the evidence supporting a potential SEP-DNAm link, we performed a systematic review of published empirical findings on the association between SEP (from prenatal development to adulthood) and DNAm measured across the life course, with an eye toward evaluating how the timing, duration, and type of SEP exposure influenced DNAm. Across the 37 studies we identified, there was some evidence for the effect of SEP timing and duration on DNAm, with early-life SEP and persistently low SEP being particularly strong indicators of DNAm. Different indicators of SEP also had some unique associations with DNAm profiles, suggesting that SEP is not a singular concept, but rather that different aspects of the socioeconomic environment can shift DNAm patterns through distinct pathways. These differences with respect to SEP timing, duration, and type were notable because they were detected even among heterogenous study designs. Overall, findings from this review underscore the importance of analyzing SEP timing, duration, and type, given the complex relationship between SEP and DNAm across the lifespan. To guide future research, we highlight current limitations in the literature and propose recommendations for overcoming some of these challenges.

scholarly journals Experiences of Social Withdrawal: Why Aging Matters Among Individuals With Hemophilia Facing Unexpected Longevity

2020 ◽  
Vol 4 (Supplement_1) ◽  
pp. 667-668
Author(s):  
Tam Perry ◽  
Sara Schwartz ◽  
Dana Francis ◽  
Charles Kaplan
Keyword(s):  
Social Withdrawal ◽  
Treatment Modalities ◽  
Telephone Interviews ◽  
Care Models ◽  
The Life Course ◽  
Donated Blood ◽  
And Coping ◽  
Long Term Survivors ◽  
First Time

Abstract For the first time, individuals with hemophilia are living beyond their 30s and 40s. This cohort, many of whom had been dependent on donated blood and contracted HIV and hepatitis, face unique challenges as they age with hemophilia and other conditions. Little is known about the experiences and health needs of these adults over the age of 40. Scientific advances have changed the life course of individuals who received earlier treatment modalities. Today, a person with hemophilia who has received preventative synthetic treatment can look forward to a long, healthy and active life. Semi-structured telephone interviews (n=32) were conducted with long-term survivors, family members, and professionals who supported these families. Findings include shame, fear and coping through social withdrawal in order to hide their hemophilia to avoid assumptions of HIV status. Analyzing unexpected longevity and why age matters for this historically isolated cohort, we explore appropriate care models.

DNA Methylation Profile of the SREBF2 Gene in Human Fetal Aortas

2021 ◽  
pp. 1-8
Author(s):  
Concetta Schiano ◽  
Maria D’Armiento ◽  
Monica Franzese ◽  
Rossana Castaldo ◽  
Gabriele Saccone ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cpg Island ◽  
Cardiovascular Prevention ◽  
Cholesterol Levels ◽  
Dna Methylation Profile ◽  
Maternal Hypercholesterolemia ◽  
Underlying Mechanisms ◽  
First Time ◽  
Methylation Profiling

Increasing evidence suggests that maternal cholesterol represents an important risk factor for atherosclerotic disease in offspring already during pregnancy, although the underlying mechanisms have not yet been elucidated. Eighteen human fetal aorta samples were collected from the spontaneously aborted fetuses of normal cholesterolemic and hypercholesterolemic mothers. Maternal total cholesterol levels were assessed during hospitalization. DNA methylation profiling of the whole <i>SREBF2</i> gene CpG island was performed (<i>p</i> value &#x3c;0.05). The Mann-Whitney U test was used for comparison between the 2 groups. For the first time, our study revealed that in fetal aortas obtained from hypercholesterolemic mothers, the <i>SREBF2</i> gene shows 4 significant differentially hypermethylated sites in the 5′UTR-CpG island. This finding indicates that more effective long-term primary cardiovascular prevention programs need to be designed for the offspring of mothers with hypercholesterolemia. Further studies should be conducted to clarify the epigenetic mechanisms underlying the association between early atherogenesis and maternal hypercholesterolemia during pregnancy.

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

An Evaluation of Provincial Macroeconomic Performance in Vietnam

2017 ◽  
pp. 34-47
Author(s):  
Hoi Le Quoc ◽  
Nam Pham Xuan ◽  
Tuan Nguyen Anh
Keyword(s):  
Development Strategy ◽  
Institutional Transformation ◽  
Economic Indicators ◽  
Macroeconomic Performance ◽  
Economic Development Strategy ◽  
Socio Economic Development ◽  
The Cost ◽  
Development Objectives ◽  
First Time

The study was targeted at developing a methodology for constructing a macroeconomic performance index at a provincial level for the first time in Vietnam based on 4 groups of measurements: (i) Economic indicators; (ii) oriented economic indicators; (iii) socio-economic indicators; and (iv) economic - social – institutional indicators. Applying the methodology to the 2011 - 2015 empirical data of all provinces in Vietnam, the research shows that the socio-economic development strategy implemented by those provinces did not provide balanced outcomes between growth and social objectives, sustainability and inclusiveness. Many provinces focused on economic growth at the cost of structural change, equality and institutional transformation. In contrast, many provinces were successful in improving equality but not growth. Those facts threaten the long-term development objectives of the provinces.

NEW VARIETIES OF CURRANTS AND GOOSEBERRY BREEDING PLANTS

1970 ◽  
pp. 41-46
Author(s):  
O. D. Golyaeva ◽  
O. V. Kurashev ◽  
S. D. Knyazev ◽  
А. Yu. Bakhotskaya
Keyword(s):  
Powdery Mildew ◽  
Genetic Basis ◽  
Black Currant ◽  
Leaf Spots ◽  
Agricultural Sciences ◽  
Berry Crops ◽  
New Varieties ◽  
First Time ◽  
Red Currant

The main goal of the scientific institution was and remains to improve the assortment of fruit and berry crops for the development of domestic horticulture. Black currant breeding at VNIISPK was started by A.F Tamarova and continued by the doctor of agricultural Sciences T.P.Ogoltsova and doctor of agricultural Sciences S.D. Knyazev. A long-term breeding program has been developed. The main goals of the program are to create black currant cultivars with continuous resistance to diseases, first of all powdery mildew, as wells resistance to pests, i.e. bud mite. As a result of the long-term work, over 40 black currant cultivars have been developed, 14 of them are zoned. Red currant breeding was led by the candidate of agricultural Sciences L.V. Bayanova; since 2001 the work has been continued by the candidate of agricultural Sciences O.D. Golyaeva. ‘Heinemanns Rote Spӓtlese’, the descendant of R. multiflorum Kit., was involved in the red currant breeding for the first time in Russia. On its genetic basis, a series of late maturing cultivars with long and dense racemes was created. At the Institute, in total 21cultivars of red currants have been developed, 13 of them are zoned. At present, red currant cultivars make up 25.5% of the zoned assortment in Russia. The first research on gooseberries was stated by V.P. Semakin and A.F Tamarova; since 1992 the systematic gooseberry breeding has been carried out by the candidate of agricultural Sciences O.V. Kurashev. On the basis of Grossularia robusta, we have created gooseberry forms that are resistant to powdery mildew and leaf spots. These forms are highly productive, weakly thorned, having bush habit suitable for mechanized harvest. The result of breeding activities was the transfer of 6 gooseberry cultivars to State agricultural testing: ‘Solnechny Zaychik’, ‘Nekrasovsky’, ‘Yupiter’, ‘Zemlianichny’, ‘Moryachok’ and ‘Discovery’.

A Review on Recent Trends in Nanomaterials and Nanocomposites for Environmental Applications

2020 ◽  
Vol 16 ◽  
Author(s):  
Kannappan Panchamoorthy Gopinath ◽  
Malolan Rajagopal ◽  
Abhishek Krishnan ◽  
Shweta Kolathur Sreerama
Keyword(s):  
Environmental Pollution ◽  
Environmental Remediation ◽  
Carbon Capture ◽  
Potential Applications ◽  
Important Challenge ◽  
Term Basis ◽  
Short And Long Term ◽  
Recent Trends ◽  
Water And Soil Pollution

Background: Depletion and contamination of environmental resources such as water, air and soil caused by human activities is an increasingly important challenge faced around the world. The consequences of environmental pollution are felt acutely by all living beings, both on a short and long-term basis, thereby making methods of remediation of environmental pollution an urgent requirement. Objectives: The objective of this review is to dissect the complications caused by environmental degradation, highlight advancements in the field of nanotechnology and to scrutinize its applications in environmental remediation. Furthermore, the review aims to concisely explain the merits and drawbacks of nanotechnology compared to existing methods. Conclusion: The current and potential applications of nanomaterials and nanocomposites in the prevention, control and reduction of air, water and soil pollution and the mechanisms involved have been elucidated, as have their various merits and demerits. The applications of nanotechnology in the fields of carbon capture and agriculture have also received attention in this review.

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