Bacillus subtilis ALBA01 can mitigate onion pink root symptoms caused by Setophoma terrestris

AbstractSoil-borne pathogen Setophoma terrestris is the causal agent of pink root of onion, one of the most challenging diseases in onion production. Conventional approaches for managing the disease like solarization, soil fumigation and crop rotation have not been proven effective enough. In this work, we evaluated the biocontrol capacity of Bacillus subtilis ALBA01 (BsA01) against S. terrestris, in a highly susceptible onion cultivar, both under greenhouse and field conditions. Disease incidence and severity were evaluated together with growth, photosynthesis among other physiological variables and yield parameters. When compared with plants infected with the pathogen, those plants co-inoculated with BsA01 showed significantly less damage and levels of biocontrol above 50%. With regard to physiological parameters, plants challenged with S terrestris and inoculated with BsA01 performed as well as the control non-infected plants revealing a growth promotion effect of BsA01 on onion plants.

Download Full-text

Control of Magnaporthe oryzae and Rice Growth Promotion by Bacillus subtilis JN005

Journal of Plant Growth Regulation ◽

10.1007/s00344-021-10444-w ◽

2021 ◽

Author(s):

Huajun Zhu ◽

Hu Zhou ◽

Zuohua Ren ◽

Erming Liu

Keyword(s):

Bacillus Subtilis ◽

Magnaporthe Oryzae ◽

Rice Blast ◽

Growth Promotion ◽

Biological Control Agent ◽

Disease Incidence ◽

Control Treatment ◽

Bacterial Suspension ◽

Sterile Water ◽

Rice Leaves

AbstractIt is quite important to develop the microorganism resources with biocontrol capacity for rice blast. This study evaluated Bacillus subtilis JN005 for growth promotion and biocontrol efficacy against Magnaporthe oryzae. Results showed that rice seeds treated with 1 × 107 cfu/mL suspension of B. subtilis JN005 had 16% germination energy, 14% germination rate, 15% germination index, and 270% vigor index compared to those treated with sterile water (control). In pot experiments, the JN005 strain-treated rice plants exhibited notable increase in plant height, root length, stem circumference, and fresh weight, as well as higher concentration of chlorophyll a, chlorophyll b, and total chlorophyll in rice leaves. Rice leaves inoculated with the JN005 strain resulted in increased activities of defense-related enzymes, including peroxidase (POD), phenylalanine ammonialyase (PAL), superoxide dismutase (SOD), and catalase (CAT) compared to the water and the M. oryzae-inoculated treatments. In vitro inoculated rice leaves with 1 × 107 cfu/mL bacterial suspension compared to sterile water or control treatment exhibited lower disease incidence in the curative and preventive groups by 79% and 76%, respectively. Field experiment showed that after spraying with 1 × 107 cfu/mL bacterial suspension, efficacy rates on controlling rice blast on plants were (56.82 ± 1.12)% and (58.39 ± 3.05)% at seedling and maturity stages, respectively, and that rice production yield was (524.40 ± 17.88) g/m2. Therefore, B. subtilis JN005 could be a promising biological control agent for rice blast, thereby warranting further investigation of its efficacy.

Download Full-text

The Enhancement of Antiviral Activity of Chloroinconazide by Aglinate-based Nanogel and Its Plant Growth Promotion Effect

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.1c00941 ◽

2021 ◽

Author(s):

Xing Lv ◽

Mengting Yuan ◽

Yuehong Pei ◽

Changyun Liu ◽

Xiangchuan Wang ◽

...

Keyword(s):

Plant Growth ◽

Antiviral Activity ◽

Plant Growth Promotion ◽

Growth Promotion ◽

Promotion Effect

Download Full-text

Transcriptomic insights into growth promotion effect of Trichoderma afroharzianum TM2-4 microbial agent on tomato plants

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(20)63415-3 ◽

2021 ◽

Vol 20 (5) ◽

pp. 1266-1276

Author(s):

Juan ZHAO ◽

Ting LIU ◽

Wei-cheng LIU ◽

Dian-peng ZHANG ◽

Dan DONG ◽

...

Keyword(s):

Growth Promotion ◽

Tomato Plants ◽

Promotion Effect ◽

Microbial Agent

Download Full-text

Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Agriculture ◽

10.3390/agriculture11010015 ◽

2020 ◽

Vol 11 (1) ◽

pp. 15

Author(s):

Sy Dinh Nguyen ◽

Thi Huyen Trang Trinh ◽

Trung Dzung Tran ◽

Tinh Van Nguyen ◽

Hoang Van Chuyen ◽

...

Keyword(s):

Bacillus Subtilis ◽

Endophytic Bacteria ◽

Growth Promotion ◽

Black Pepper ◽

Rhizosphere Bacteria ◽

Growth And Yield ◽

Piper Nigrum ◽

Bacillus Velezensis ◽

Root Knot Nematode ◽

Central Highland

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

Download Full-text

The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽

10.3390/agronomy11050888 ◽

2021 ◽

Vol 11 (5) ◽

pp. 888

Author(s):

Giorgia Novello ◽

Patrizia Cesaro ◽

Elisa Bona ◽

Nadia Massa ◽

Fabio Gosetti ◽

...

Keyword(s):

Plant Growth ◽

Growth Promotion ◽

Global Market ◽

Plant Growth Promoting Bacteria ◽

Bacterial Strains ◽

Mineral Concentration ◽

Positive Effects ◽

Plant Growth Promoting ◽

Onion Cultivar ◽

Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

Download Full-text

A note on the antibiotic properties of Bacillus subtilis against Colletotrichum trifolii

Phytoprotection ◽

10.7202/706018ar ◽

2005 ◽

Vol 73 (1) ◽

pp. 31-36 ◽

Cited By ~ 7

Author(s):

Y. Douville ◽

J.G. Boland

Keyword(s):

Bacillus Subtilis ◽

Disease Incidence ◽

Suppressive Effect ◽

Mechanisms Of Action ◽

Colletotrichum Trifolii ◽

Growth Room ◽

A Cell ◽

Germ Tubes

The influence and mechanisms of action of Bacillus subtilis on Colletotrichum trifolii, a causal agent of anthracnose of alfalfa (Medicago sativa), were studied in vivo and in vitro. In growth room conditions, a cell-free culture filtrate of B. subtilis significantly reduced disease incidence and severity on alfalfa seedlings from 56% to 16% and from 2.0 to 1.2, respectively. Treatment of seedlings with washed cell suspensions of B. subtilis had no influence on disease. Applications of crude filtrate on alfalfa leaflets inoculated with C. trifolii were associated with reduced germination of conidia, lysis of conidia, and reduced formation of appressoria. Under in vitro conditions, crude filtrate reduced germination of conidia, and induced lysis of conidia and the formation of inflated germ tubes on germinating conidia. An antibiotic of the iturin family, iturin D, was tentatively identified as the active compound responsible for the suppressive effect of B. subtilis on C. trifolii.

Download Full-text

Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop

Arab Journal Of Plant Protection ◽

10.22268/ajpp-039.1.079083 ◽

2021 ◽

Vol 39 (1) ◽

pp. 79-83

Author(s):

Yasir Iftikhar ◽

◽

Mustansar Mubeen ◽

Ashara Sajid ◽

Mohamed Ahmad Zeshan ◽

...

Keyword(s):

Disease Severity ◽

Disease Incidence ◽

Tomato Leaf ◽

Growth And Yield ◽

Tomato Crop ◽

Tomato Leaf Curl Virus ◽

Yield Parameters ◽

Tomato Leaf Curl ◽

Leaf Curl Virus ◽

Leaf Curl

Iftikhar, Y., M. Mubeen, A. Sajid, M.A. Zeshan, Q. Shakeel, A. Abbas, S. Bashir, M. Kamran and H. Anwaar. 2021. Effects of Tomato Leaf Curl Virus on Growth and Yield Parameters of Tomato Crop. Arab Journal of Plant Protection, 39(1): 79-83. Tomato is an important vegetable crop, belongs to the family Solanaceae and is the second most consumed vegetable following potatoes. The tomato crop is grown all over the world in both summer and winter seasons, and plant viruses are a major threat to tomato production. Among these viruses, tomato leaf curl virus (TLCV) causes considerable yield loss to tomato crop. This virus is transmitted by a whitefly (Bemisia tabaci) vector. In this study, the effect of TLCV infection, on the following tomato growth and yield parameters, was evaluated: plant leaf number and area, plant biomass, plant height, root length, and plant stem diameter and yield. Tomato plants were transplanted in wellprepared plots with 4 replications. The control group was covered with polyethene bag to avoid whitefly infestation. Plants were scored on the 15th and 30th day after inoculation and TLCV disease severity was recorded. Analysis of variance (ANOVA) showed the significant differences between the healthy and infected tomato plants. Moreover, growth and yield parameters were reduced with the increase in disease incidence, disease severity and whitefly infestation. Disease severity was increased with the increase in temperature during the growing season. It can be concluded from this study that TLCV significantly affects growth and yield of the tomato crop. Keywords: Tomato, Tomato leaf curl virus, TLCV, disease incidence, disease severity.

Download Full-text