scholarly journals Neuronal avalanches in input and associative layers of auditory cortex

2019 ◽  
Author(s):  
Zac Bowen ◽  
Daniel E. Winkowski ◽  
Saurav Seshadri ◽  
Dietmar Plenz ◽  
Patrick O. Kanold
Keyword(s):  
Auditory Cortex ◽  
Information Transfer ◽  
Dynamic Range ◽  
Speech Sound ◽  
Power Laws ◽  
Single Frequency ◽  
Population Activity ◽  
Sound Levels ◽  
Neuronal Avalanches

AbstractThe primary auditory cortex processes acoustic sequences for the perception of behaviorally meaningful sounds such as speech. Sound information arrives at its input layer 4 from where activity propagates to associative layer 2/3. It is currently not known whether there is a particular organization of neuronal population activity that is stable across layers and sound levels during sound processing. We used in vivo 2-photon imaging of pyramidal neurons in cortical layers L4 and L2/3 of mouse A1 to characterize the populations of neurons that were active spontaneously, i.e. in the absence of a sound stimulus, and those recruited by single-frequency tonal stimuli at different sound levels. Single-frequency sounds recruited neurons of widely ranging frequency selectivity in both layers. We defined neural ensembles as neurons being active within or during successive temporal windows at the temporal resolution of our imaging. For both layers, neuronal ensembles were highly variable in size during spontaneous activity as well as during sound presentation. Ensemble sizes distributed according to power laws, the hallmark of neuronal avalanches, and were similar across sound levels. Avalanches activated by sound were composed of neurons with diverse tuning preference, yet with selectivity independent of avalanche size. Thus, spontaneous and evoked activity in both L4 and L2/3 of A1 are composed of neuronal avalanches with similar power law relationships. Our results demonstrate network principles linked to maximal dynamic range, optimal information transfer and matching complexity between L4 and L2/3 to shape population activity in auditory cortex.

scholarly journals L-type calcium channels refine the neural population code of sound level

2016 ◽  
Vol 116 (6) ◽  
pp. 2550-2563 ◽  
Author(s):  
Calum Alex Grimsley ◽  
David Brian Green ◽  
Shobhana Sivaramakrishnan
Keyword(s):  
Calcium Channels ◽  
Calcium Current ◽  
Dynamic Range ◽  
Sound Level ◽  
Neural Population ◽  
Total Calcium ◽  
Local Networks ◽  
Sound Levels ◽  
Local Circuits

The coding of sound level by ensembles of neurons improves the accuracy with which listeners identify how loud a sound is. In the auditory system, the rate at which neurons fire in response to changes in sound level is shaped by local networks. Voltage-gated conductances alter local output by regulating neuronal firing, but their role in modulating responses to sound level is unclear. We tested the effects of L-type calcium channels (CaL: CaV1.1–1.4) on sound-level coding in the central nucleus of the inferior colliculus (ICC) in the auditory midbrain. We characterized the contribution of CaL to the total calcium current in brain slices and then examined its effects on rate-level functions (RLFs) in vivo using single-unit recordings in awake mice. CaL is a high-threshold current and comprises ∼50% of the total calcium current in ICC neurons. In vivo, CaL activates at sound levels that evoke high firing rates. In RLFs that increase monotonically with sound level, CaL boosts spike rates at high sound levels and increases the maximum firing rate achieved. In different populations of RLFs that change nonmonotonically with sound level, CaL either suppresses or enhances firing at sound levels that evoke maximum firing. CaL multiplies the gain of monotonic RLFs with dynamic range and divides the gain of nonmonotonic RLFs with the width of the RLF. These results suggest that a single broad class of calcium channels activates enhancing and suppressing local circuits to regulate the sensitivity of neuronal populations to sound level.

scholarly journals Irregular spiking of pyramidal neurons organizes as scale-invariant neuronal avalanches in the awake state

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Timothy Bellay ◽  
Andreas Klaus ◽  
Saurav Seshadri ◽  
Dietmar Plenz
Keyword(s):  
Pyramidal Neurons ◽  
Physiological State ◽  
Scale Invariant ◽  
Population Activity ◽  
Spatial And Temporal Scales ◽  
Awake State ◽  
Resting Activity ◽  
Neuronal Avalanches

Spontaneous fluctuations in neuronal activity emerge at many spatial and temporal scales in cortex. Population measures found these fluctuations to organize as scale-invariant neuronal avalanches, suggesting cortical dynamics to be critical. Macroscopic dynamics, though, depend on physiological states and are ambiguous as to their cellular composition, spatiotemporal origin, and contributions from synaptic input or action potential (AP) output. Here, we study spontaneous firing in pyramidal neurons (PNs) from rat superficial cortical layers in vivo and in vitro using 2-photon imaging. As the animal transitions from the anesthetized to awake state, spontaneous single neuron firing increases in irregularity and assembles into scale-invariant avalanches at the group level. In vitro spike avalanches emerged naturally yet required balanced excitation and inhibition. This demonstrates that neuronal avalanches are linked to the global physiological state of wakefulness and that cortical resting activity organizes as avalanches from firing of local PN groups to global population activity.

scholarly journals POS1388 ULTRASOUND FOR PANNICULITIS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 977.1-977
Author(s):  
A. Potapova ◽  
O. Egorova ◽  
O. Alekseeva ◽  
A. Volkov ◽  
S. Radenska-Lopovok
Keyword(s):  
Dynamic Range ◽  
Subcutaneous Fat ◽  
Diagnostic Value ◽  
High Energy ◽  
Contralateral Side ◽  
Imaging Method ◽  
Non Invasive ◽  
M 12

Background:Ultrasound (US) is a non-invasive and safe imaging method that allows in vivo differentiation of the morphological structures of subcutaneous fat (SCF) tissue in in normal and pathology.Objectives:Reveal features of ultrasound changes in SCF in panniculitis (Pn).Methods:57 patients (f – 45, m - 12) aged 18 - 67 years with an initial diagnosis of erythema nodosum and a disease duration of 3.6 ± 1.4 years were examined. In addition to the general clinical examination, a computed tomography of the chest organs and a pathomorphological examination of a skin biopsy from the site of the node were performed. Ultrasound was performed on a MyLabTwice apparatus (ESAOTE, Italy) using a multi-frequency linear transducer (10-18 MHz) with the PD technique, the parameters of which were adapted for recording low-speed flows (PRF 300-600 Hz, low filter, dynamic range - 20-40 dB), the presence of vascularization was assessed not only in the affected area, but also on the contralateral side using high-energy Doppler.Results:33 patients were diagnosed with septal Pn (SPn), 24 - lobular Pn (LPn). In all cases, the diagnosis was verified by histological examination. Ultrasound made it possible to assess the thickness, echoicity and vascularization of the SCF. In 35 patients, significant thickening of the SCF was revealed (as compared to the contralateral side), of which in 14 cases with SPn, in 21 - with LPn. Significant diffuse thickening of the SCF with the contralateral side was observed in 18 patients, incl. in 12 (66%) patients with LPn. Limited thickening was more typical for SPn (73%). A significant increase in the echoicity of the SCF was noted in all forms of Pn. A “lobular” echo pattern with an anechogenic environment was observed in 25 patients, of which 18 (72%) had LPn. An increase in vascularization compared to the contralateral side was recorded in 30 cases (SPn-17, LPn-13).Conclusion:The obtained preliminary results indicate the important role of ultrasound in assessing the depth and prevalence of the inflammatory process at Pn. To clarify the diagnostic value of this method, further studies are needed on a larger sample of patients.Disclosure of Interests:None declared

Airborne resistivity and susceptibility mapping in magnetically polarizable areas

Geophysics ◽  
2000 ◽  
Vol 65 (2) ◽  
pp. 502-511 ◽  
Author(s):  
Haoping Huang ◽  
Douglas C. Fraser
Keyword(s):  
Half Space ◽  
Magnetic Permeability ◽  
Apparent Resistivity ◽  
Dynamic Range ◽  
Single Frequency ◽  
Measured Signal ◽  
Apparent Permeability ◽  
Quadrature Component ◽  
New Methods ◽  
Quadrature Response

The apparent resistivity technique using half‐space models has been employed in helicopter‐borne resistivity mapping for twenty years. These resistivity algorithms yield the apparent resistivity from the measured in‐phase and quadrature response arising from the flow of electrical conduction currents for a given frequency. However, these algorithms, which assume free‐space magnetic permeability, do not yield a reliable value for the apparent resistivity in highly magnetic areas. This is because magnetic polarization also occurs, which modifies the electromagnetic (EM) response, causing the computed resistivity to be erroneously high. Conversely, the susceptibility of a magnetic half‐space can be computed from the measured EM response, assuming an absence of conduction currents. However, the presence of conduction currents will cause the computed susceptibility to be erroneously low. New methods for computing the apparent resistivity and apparent magnetic permeability have been developed for the magnetic conductive half‐space. The in‐phase and quadrature responses at the lowest frequency are first used to estimate the apparent magnetic permeability. The lowest frequency should be used to calculate the permeability because this minimizes the contribution to the measured signal from conduction currents. Knowing the apparent magnetic permeability then allows the apparent resistivity to be computed for all frequencies. The resistivity can be computed using different methods. Because the EM response of magnetic permeability is much greater for the in‐phase component than for the quadrature component, it may be better in highly magnetic environments to derive the resistivity using the quadrature component at two frequencies (the quad‐quad algorithm) rather than using the in‐phase and quadrature response at a single frequency (the in‐phase‐quad algorithm). However, the in‐phase‐quad algorithm has the advantage of dynamic range, and it gives credible resistivity results when the apparent permeability has been obtained correctly.

Influence of Tracheal Obstruction on the Efficacy of Superimposed High-frequency Jet Ventilation and Single-frequency Jet Ventilation

2015 ◽  
Vol 123 (4) ◽  
pp. 799-809 ◽  
Author(s):  
Robert Sütterlin ◽  
Antonella LoMauro ◽  
Stefano Gandolfi ◽  
Rita Priori ◽  
Andrea Aliverti ◽  
...  
Keyword(s):  
Gas Exchange ◽  
Chest Wall ◽  
High Frequency ◽  
Blood Gases ◽  
Single Frequency ◽  
Jet Ventilation ◽  
Lung Volumes ◽  
High Frequency Jet Ventilation ◽  
Tracheal Obstruction

Abstract Background: Both superimposed high-frequency jet ventilation (SHFJV) and single-frequency (high-frequency) jet ventilation (HFJV) have been used with success for airway surgery, but SHFJV has been found to provide higher lung volumes and better gas exchange than HFJV in unobstructed airways. The authors systematically compared the ventilation efficacy of SHFJV and HFJV at different ventilation frequencies in a model of tracheal obstruction and describe the frequency and obstruction dependence of SHFJV efficacy. Methods: Ten anesthetized animals (weight 25 to 31.5 kg) were alternately ventilated with SHFJV and HFJV at a set of different fHF from 50 to 600 min−1. Obstruction was created by insertion of interchangeable stents with ID 2 to 8 mm into the trachea. Chest wall volume was measured using optoelectronic plethysmography, airway pressures were recorded, and blood gases were analyzed repeatedly. Results: SHFJV provided greater than 1.6 times higher end-expiratory chest wall volume than HFJV, and tidal volume (VT) was always greater than 200 ml with SHFJV. Increase of fHF from 50 to 600 min−1 during HFJV resulted in a more than 30-fold VT decrease from 112 ml (97 to 130 ml) to negligible values and resulted in severe hypoxia and hypercapnia. During SHFJV, stent ID reduction from 8 to 2 mm increased end-expiratory chest wall volume by up to 3 times from approximately 100 to 300 ml and decreased VT by up to 4.2 times from approximately 470 to 110 ml. Oxygenation and ventilation were acceptable for 4 mm ID or more, but hypercapnia occurred with the 2 mm stent. Conclusion: In this in vivo porcine model of variable severe tracheal stenosis, SHFJV effectively increased lung volumes and maintained gas exchange and may be advantageous in severe airway obstruction.

Medial Olivocochlear Reflex Effects on Amplitude Growth Functions of Long- and Short-Latency Components of Click-Evoked Otoacoustic Emissions in Humans

2021 ◽  
Author(s):  
Shawn Goodman ◽  
Sriram Boothalingam ◽  
Jeffery T Lichtenhan
Keyword(s):  
Otoacoustic Emissions ◽  
Dynamic Range ◽  
Short Latency ◽  
Medial Olivocochlear Reflex ◽  
Time Frequency ◽  
Growth Functions ◽  
Sound Levels ◽  
Amplitude Attenuation ◽  
Slope Change ◽  
Medial Olivocochlear

Functional outcomes of medial olivocochlear reflex (MOCR) activation, such as improved hearing in background noise and protection from noise damage, involve moderate to high sound levels. Previous noninvasive measurements of MOCR in humans focused primarily on otoacoustic emissions (OAEs) evoked at low sound levels. Interpreting MOCR effects on OAEs at higher levels is complicated by the possibility of the middle-ear muscle reflex and by components of OAEs arising from different locations along the length of the cochlear spiral. We overcame these issues by presenting click stimuli at a very slow rate and by time-frequency windowing the resulting click-evoked (CE)OAEs into short-latency (SL) and long-latency (LL) components. We characterized the effects of MOCR on CEOAE components using multiple measures to more comprehensively assess these effects throughout much of the dynamic range of hearing. These measures included CEOAE amplitude attenuation, equivalent input attenuation, phase, and slope of growth functions. Results show that MOCR effects are smaller on SL components than LL components, consistent with SL components being generated slightly basal of the characteristic frequency region. Amplitude attenuation measures showed the largest effects at the lowest stimulus levels, but slope change and equivalent input attenuation measures did not decrease at higher stimulus levels. These latter measures are less commonly reported and may provide insight into the variability in listening performance and noise susceptibility seen across individuals.

scholarly journals Methods for Voltage-Sensitive Dye Imaging of Rat Cortical Activity With High Signal-to-Noise Ratio

2007 ◽  
Vol 98 (1) ◽  
pp. 502-512 ◽  
Author(s):  
Michael T. Lippert ◽  
Kentaroh Takagaki ◽  
Weifeng Xu ◽  
Xiaoying Huang ◽  
Jian-Young Wu
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Field Potential ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
High Signal ◽  
Blue Dye ◽  
Voltage Sensitive Dye ◽  
Imaging Device

We describe methods to achieve high sensitivity in voltage-sensitive dye (VSD) imaging from rat barrel and visual cortices in vivo with the use of a blue dye RH1691 and a high dynamic range imaging device (photodiode array). With an improved staining protocol and an off-line procedure to remove pulsation artifact, the sensitivity of VSD recording is comparable with that of local field potential recording from the same location. With this sensitivity, one can record from ∼500 individual detectors, each covering an area of cortical tissue 160 μm in diameter (total imaging field ∼4 mm in diameter) and a temporal resolution of 1,600 frames/s, without multiple-trial averaging. We can record 80–100 trials of intermittent 10-s trials from each imaging field before the VSD signal reduces to one half of its initial amplitude because of bleaching and wash-out. Taken together, the methods described in this report provide a useful tool for visualizing evoked and spontaneous waves from rodent cortex.

Sign in / Sign up

Export Citation Format

Share Document