scholarly journals Methods for Voltage-Sensitive Dye Imaging of Rat Cortical Activity With High Signal-to-Noise Ratio

2007 ◽  
Vol 98 (1) ◽  
pp. 502-512 ◽  
Author(s):  
Michael T. Lippert ◽  
Kentaroh Takagaki ◽  
Weifeng Xu ◽  
Xiaoying Huang ◽  
Jian-Young Wu
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Field Potential ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
High Signal ◽  
Blue Dye ◽  
Voltage Sensitive Dye ◽  
Imaging Device

We describe methods to achieve high sensitivity in voltage-sensitive dye (VSD) imaging from rat barrel and visual cortices in vivo with the use of a blue dye RH1691 and a high dynamic range imaging device (photodiode array). With an improved staining protocol and an off-line procedure to remove pulsation artifact, the sensitivity of VSD recording is comparable with that of local field potential recording from the same location. With this sensitivity, one can record from ∼500 individual detectors, each covering an area of cortical tissue 160 μm in diameter (total imaging field ∼4 mm in diameter) and a temporal resolution of 1,600 frames/s, without multiple-trial averaging. We can record 80–100 trials of intermittent 10-s trials from each imaging field before the VSD signal reduces to one half of its initial amplitude because of bleaching and wash-out. Taken together, the methods described in this report provide a useful tool for visualizing evoked and spontaneous waves from rodent cortex.

scholarly journals Ab initiostructure determination of nanocrystals of organic pharmaceutical compounds by electron diffraction at room temperature using a Timepix quantum area direct electron detector

2016 ◽  
Vol 72 (2) ◽  
pp. 236-242 ◽  
Author(s):  
E. van Genderen ◽  
M. T. B. Clabbers ◽  
P. P. Das ◽  
A. Stewart ◽  
I. Nederlof ◽  
...  
Keyword(s):  
Electron Diffraction ◽  
Room Temperature ◽  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Three Dimensional ◽  
Direct Methods ◽  
Liquid Nitrogen Temperature ◽  
High Dynamic Range ◽  
Electron Diffraction Data ◽  
High Signal

Until recently, structure determination by transmission electron microscopy of beam-sensitive three-dimensional nanocrystals required electron diffraction tomography data collection at liquid-nitrogen temperature, in order to reduce radiation damage. Here it is shown that the novel Timepix detector combines a high dynamic range with a very high signal-to-noise ratio and single-electron sensitivity, enablingab initiophasing of beam-sensitive organic compounds. Low-dose electron diffraction data (∼0.013 e− Å−2 s−1) were collected at room temperature with the rotation method. It was ascertained that the data were of sufficient quality for structure solution using direct methods using software developed for X-ray crystallography (XDS,SHELX) and for electron crystallography (ADT3D/PETS,SIR2014).

A theoretical study of digital silicon photomultiplier utilization in diffuse optical imaging systems

2019 ◽  
Vol 64 (3) ◽  
pp. 357-363 ◽  
Author(s):  
Taha Haddadifam ◽  
Mohammad Azim Karami
Keyword(s):  
Optical Imaging ◽  
Continuous Wave ◽  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
Diffuse Optical Imaging ◽  
Silicon Photomultiplier ◽  
Thoracic Imaging ◽  
Low Level

Abstract Digital silicon photomultiplier (dSiPM) is introduced for diffuse optical imaging (DOI) applications instead of conventional photomultiplier tubes and avalanche photodiodes (APDs) as a state-of-the-art detector. According to the low-level light regime in DOI applications, high sensitivity and high dynamic range (DR) image sensors are needed for DOI systems. dSiPM is proposed as a developing detector which can detect low-level lights. Also, an accurate equation is obtained for calculating the DR of dSiPMs. Different dSiPMs and the corresponding benefits are studied for DOI applications. Furthermore, a 120 dB DR dSiPM is chosen for use in DOI systems. It is shown that dSiPMs can be utilized in DOI configurations such as time domain (TD), frequency domain (FD) and continuous wave (CW) systems. Ultimately, by utilizing dSiPM in DOI systems, the DOI method can be used for thoracic imaging due to the high DR and signal-to-noise ratio (SNR) of the detector.

scholarly journals High sensitivity fluorescence up-conversion spectroscopy of 3MLCT emission of metal-organic complexes

2019 ◽  
Vol 205 ◽  
pp. 09009 ◽  
Author(s):  
Li Liu ◽  
Damianos Agathangelou ◽  
Thomas Roland ◽  
Olivier Crégut ◽  
Thibaud Duchanois ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Emission Spectra ◽  
High Sensitivity ◽  
High Signal ◽  
Triplet States ◽  
Organic Complexes ◽  
Metal Organic ◽  
Set Up ◽  
Weak Luminescence

We demonstrate the implementation of a broadband fluorescence up-conversion set-up with high signal-to-noise ratio and dynamic range allowing for the detection of weak luminescence from triplet states in Fe(II) NHC complexes. Based on the experimentally determined radiative rates and the emission spectra, these states have dominant MLCT character.

scholarly journals Genetically Encoded Fluorescent Indicators for Imaging Brain Chemistry

Biosensors ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 116
Author(s):  
Xiaoke Bi ◽  
Connor Beck ◽  
Yiyang Gong
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
Spatial Scales ◽  
High Signal ◽  
Spatiotemporal Resolution ◽  
Brain Chemistry ◽  
Fluorescent Indicators ◽  
Recent Developments

Genetically encoded fluorescent indicators, combined with optical imaging, enable the detection of physiologically or behaviorally relevant neural activity with high spatiotemporal resolution. Recent developments in protein engineering and screening strategies have improved the dynamic range, kinetics, and spectral properties of genetically encoded fluorescence indicators of brain chemistry. Such indicators have detected neurotransmitter and calcium dynamics with high signal-to-noise ratio at multiple temporal and spatial scales in vitro and in vivo. This review summarizes the current trends in these genetically encoded fluorescent indicators of neurotransmitters and calcium, focusing on their key metrics and in vivo applications.

scholarly journals Ultrahigh-speed point scanning two-photon microscopy using high dynamic range silicon photomultipliers

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vincent D. Ching-Roa ◽  
Eben M. Olson ◽  
Sherrif F. Ibrahim ◽  
Richard Torres ◽  
Michael G. Giacomelli
Keyword(s):  
High Speed ◽  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
Photomultiplier Tubes ◽  
Silicon Photomultipliers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Detection Technology

AbstractConventional two-photon microscopes use photomultiplier tubes, which enable high sensitivity but can detect relatively few photons per second, forcing longer pixel integration times and limiting maximum imaging rates. We introduce novel detection electronics using silicon photomultipliers that greatly extend dynamic range, enabling more than an order of magnitude increased photon detection rate as compared to state-of-the-art photomultiplier tubes. We demonstrate that this capability can dramatically improve both imaging rates and signal-to-noise ratio (SNR) in two-photon microscopy using human surgical specimens. Finally, to enable wider use of more advanced detection technology, we have formed the OpenSiPM project, which aims to provide open source detector designs for high-speed two-photon and confocal microscopy.

scholarly journals Synchrotron X-ray diffraction experiments with a prototype hybrid pixel detector

2011 ◽  
Vol 45 (1) ◽  
pp. 38-47 ◽  
Author(s):  
C. Le Bourlot ◽  
P. Landois ◽  
S. Djaziri ◽  
P.-O. Renault ◽  
E. Le Bourhis ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Signal To Noise Ratio ◽  
High Dynamic Range ◽  
High Signal ◽  
Pixel Detector ◽  
X Ray Diffraction ◽  
X Ray ◽  
Area Detector ◽  
Duplex Steel ◽  
Scientific Results

A prototype X-ray pixel area detector (XPAD3.1) has been used for X-ray diffraction experiments with synchrotron radiation. The characteristics of this detector are very attractive in terms of fast readout time, high dynamic range and high signal-to-noise ratio. The prototype XPAD3.1 enabled various diffraction experiments to be performed at different energies, sample-to-detector distances and detector angles with respect to the direct beam, yet it was necessary to perform corrections on the diffraction images according to the type of experiment. This paper is focused on calibration and correction procedures to obtain high-quality scientific results specifically developed in the context of three different experiments, namely mechanical characterization of nanostructured multilayers, elastic–plastic deformation of duplex steel and growth of carbon nanotubes.

scholarly journals Bioluminescent Sensors for Ca++ Flux Imaging and the Introduction of a New Intensity-Based Ca++ Sensor

2021 ◽  
Vol 9 ◽  
Author(s):  
Jie Yang ◽  
Carl Hirschie Johnson
Keyword(s):  
Dynamic Range ◽  
Cultured Cells ◽  
Signal To Noise Ratio ◽  
Dynamic Change ◽  
Living Cells ◽  
High Signal ◽  
Pros And Cons ◽  
Rapid Kinetics

Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs) within living cells or intact organisms in vivo. An ideal GECI would exhibit high signal intensity, excellent signal-to-noise ratio (SNR), rapid kinetics, a large dynamic range within relevant physiological conditions, and red-shifted emission. Most available GECIs are based on fluorescence, but bioluminescent GECIs have potential advantages in terms of avoiding tissue autofluorescence, phototoxicity, photobleaching, and spectral overlap, as well as enhancing SNR. Here, we summarize current progress in the development of bioluminescent GECIs and introduce a new and previously unpublished biosensor. Because these biosensors require a substrate, we also describe the pros and cons of various substrates used with these sensors. The novel GECI that is introduced here is called CalBiT, and it is a Ca++ indicator based on the functional complementation of NanoBiT which shows a high dynamic change in response to Ca++ fluxes. Here, we use CalBiT for the detection of Ca++ fluctuations in cultured cells, including its ability for real-time imaging in living cells.

Development of imaging plate for a TEM and its characteristics

1989 ◽  
Vol 47 ◽  
pp. 100-101
Author(s):  
N. Mori ◽  
T. Oikawa ◽  
Y. Harada ◽  
J. Miyahara ◽  
T. Matsuo
Keyword(s):  
Dynamic Range ◽  
Protective Layer ◽  
High Sensitivity ◽  
Imaging Plate ◽  
Phosphor Layer ◽  
Imaging Device ◽  
X Ray Imaging ◽  
New Type ◽  
Basic Characteristics ◽  
Reading System

The Imaging Plate (IP) is a new type imaging device, which was developed for diagnostic x ray imaging. We have reported that usage of the IP for a TEM has many merits; those are high sensitivity, wide dynamic range, and good linearity. However in the previous report the reading system was prototype drum-type-scanner, and IP was also experimentally made, which phosphor layer was 50μm thick with no protective layer. So special care was needed to handle them, and they were used only to make sure the basic characteristics. In this article we report the result of newly developed reading, printing system and high resolution IP for practical use. We mainly discuss the characteristics of the IP here. (Precise performance concerned with the reader and other system are reported in the other article.)Fig.1 shows the schematic cross section of the IP. The IP consists of three parts; protective layer, phosphor layer and support.

scholarly journals High-frequency rectifiers based on type-II Dirac fermions

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Libo Zhang ◽  
Zhiqingzi Chen ◽  
Kaixuan Zhang ◽  
Lin Wang ◽  
Huang Xu ◽  
...  
Keyword(s):  
High Frequency ◽  
Dynamic Range ◽  
High Sensitivity ◽  
High Dynamic Range ◽  
Anisotropy Ratio ◽  
Dirac Fermions ◽  
Topological States ◽  
Topological Semimetals ◽  
Polarized Surface ◽  
Symmetry Protected

AbstractThe advent of topological semimetals enables the exploitation of symmetry-protected topological phenomena and quantized transport. Here, we present homogeneous rectifiers, converting high-frequency electromagnetic energy into direct current, based on low-energy Dirac fermions of topological semimetal-NiTe2, with state-of-the-art efficiency already in the first implementation. Explicitly, these devices display room-temperature photosensitivity as high as 251 mA W−1 at 0.3 THz in an unbiased mode, with a photocurrent anisotropy ratio of 22, originating from the interplay between the spin-polarized surface and bulk states. Device performances in terms of broadband operation, high dynamic range, as well as their high sensitivity, validate the immense potential and unique advantages associated to the control of nonequilibrium gapless topological states via built-in electric field, electromagnetic polarization and symmetry breaking in topological semimetals. These findings pave the way for the exploitation of topological phase of matter for high-frequency operations in polarization-sensitive sensing, communications and imaging.

scholarly journals Mid-infrared photoacoustic gas monitoring driven by a gas-filled hollow-core fiber laser

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yazhou Wang ◽  
Yuyang Feng ◽  
Abubakar I. Adamu ◽  
Manoj K. Dasa ◽  
J. E. Antonio-Lopez ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Signal To Noise Ratio ◽  
High Sensitivity ◽  
Laser Source ◽  
High Signal ◽  
Mid Infrared ◽  
Raman Fiber Laser ◽  
Custom Made ◽  
Core Fiber ◽  
Detection Technologies

AbstractDevelopment of novel mid-infrared (MIR) lasers could ultimately boost emerging detection technologies towards innovative spectroscopic and imaging solutions. Photoacoustic (PA) modality has been heralded for years as one of the most powerful detection tools enabling high signal-to-noise ratio analysis. Here, we demonstrate a novel, compact and sensitive MIR-PA system for carbon dioxide (CO2) monitoring at its strongest absorption band by combining a gas-filled fiber laser and PA technology. Specifically, the PA signals were excited by a custom-made hydrogen (H2) based MIR Raman fiber laser source with a pulse energy of ⁓ 18 μJ, quantum efficiency of ⁓ 80% and peak power of ⁓ 3.9 kW. A CO2 detection limit of 605 ppbv was attained from the Allan deviation. This work constitutes an alternative method for advanced high-sensitivity gas detection.

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