Genome-wide Transcriptomic Analysis of the Response of Botrytis cinerea to Wuyiencin

AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

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Direct antagonistic activity of chitinase produced by Trichoderma sp. SANA20 as biological control agent for grey mould caused by Botrytis cinerea

Cogent Biology ◽

10.1080/23312025.2020.1747903 ◽

2020 ◽

Vol 6 (1) ◽

pp. 1747903 ◽

Cited By ~ 1

Author(s):

Yoshinao Aoki ◽

Sanami Haga ◽

Shunji Suzuki ◽

Eugenio Llorens

Keyword(s):

Biological Control ◽

Botrytis Cinerea ◽

Biological Control Agent ◽

Grey Mould ◽

Control Agent ◽

Antagonistic Activity ◽

Trichoderma Sp

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Hybrid Genome Assembly and Evidence-Based Annotation of the Egg Parasitoid and Biological Control Agent Trichogramma brassicae

G3 Genes|Genome|Genetics ◽

10.1534/g3.120.401344 ◽

2020 ◽

Vol 10 (10) ◽

pp. 3533-3540 ◽

Cited By ~ 1

Author(s):

Kim B. Ferguson ◽

Tore Kursch-Metz ◽

Eveline C. Verhulst ◽

Bart A. Pannebakker

Keyword(s):

Biological Control ◽

De Novo ◽

Biological Control Agent ◽

Egg Parasitoid ◽

Control Agent ◽

Evidence Based ◽

Trichogramma Brassicae ◽

A Genome ◽

Hybrid Genome ◽

Essential Resource

Trichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and sequenced in a hybrid de novo strategy, after which several assembling strategies were evaluated. The best assembly, derived from a DBG2OLC-based pipeline, yielded a genome of 235 Mbp made up of 1,572 contigs with an N50 of 556,663 bp. Following a rigorous ab initio-, homology-, and evidence-based annotation, 16,905 genes were annotated and functionally described. As an example of the utility of the genome, a simple ortholog cluster analysis was performed with sister species T. pretiosum, revealing over 6000 shared clusters and under 400 clusters unique to each species. The genome and transcriptome presented here provides an essential resource for comparative genomics of the commercially relevant genus Trichogramma, but also for research into molecular evolution, ecology, and breeding of T. brassicae.

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Hybrid genome assembly and evidence-based annotation of the egg parasitoid and biological control agent Trichogramma brassicae

10.1101/2020.04.06.027094 ◽

2020 ◽

Author(s):

K. B. Ferguson ◽

T. Kursch-Metz ◽

E. C. Verhulst ◽

B. A. Pannebakker

Keyword(s):

Biological Control ◽

De Novo ◽

Biological Control Agent ◽

Egg Parasitoid ◽

Control Agent ◽

Evidence Based ◽

Trichogramma Brassicae ◽

A Genome ◽

Hybrid Genome ◽

Essential Resource

ABSTRACTTrichogramma brassicae (Bezdenko) are egg parasitoids that are used throughout the world as biological control agents and in laboratories as model species. Despite this ubiquity, few genetic resources exist beyond COI, ITS2, and RAPD markers. Aided by a Wolbachia infection, a wild-caught strain from Germany was reared for low heterozygosity and sequenced in a hybrid de novo strategy, after which several assembling strategies were evaluated. The best assembly, derived from a DBG2OLC-based pipeline, yielded a genome of 235 Mbp made up of 1,572 contigs with an N50 of 556,663 bp. Following a rigorous ab initio-, homology-, and evidence-based annotation, 16,905 genes were annotated and functionally described. As an example of the utility of the genome, a simple ortholog cluster analysis was performed with sister species T. pretiosum, revealing over 6000 shared clusters and under 400 clusters unique to each species. The genome and transcriptome presented here provides an essential resource for comparative genomics of the commercially relevant genus Trichogramma, but also for research into molecular evolution, ecology, and breeding of T. brassicae.

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